bhf-ucl PML-IV functions as a negative regulator of telomerase by interacting with TERT. PML-IV functions as a negative regulator of telomerase by interacting with TERT. J. Cell. Sci. (0021-9533) 2009 imex curation Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging anti-tag immunocytochemistry anti-tag immunocytochemistry Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu Accepted 2013-SEP-23 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2009 imex curation PML-IV functions as a negative regulator of telomerase by interacting with TERT. PML-IV functions as a negative regulator of telomerase by interacting with TERT. J. Cell. Sci. (0021-9533) 2009 imex curation Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Figure 3 C was not added because all the mutants were able to bind i.e. no change in binding. Figure 3 D was not added because mutants that are resistant to SUMOylation were also able to bind i.e. no change in binding. Figure 3 B was not added as is redundant with Figure 3 E (latter already added). Where 3 B shows that deletion mutants do not bind and 3 E shows the equivalent deleted length to be required to bind. Is this correct? Origin of TERT inferred from sequence length jso678@skku.edu Accepted 2013-SEP-23 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2009 imex curation PML-IV functions as a negative regulator of telomerase by interacting with TERT. PML-IV functions as a negative regulator of telomerase by interacting with TERT. J. Cell. Sci. (0021-9533) 2009 imex curation Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu Accepted 2013-SEP-23 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2009 imex curation PML-IV functions as a negative regulator of telomerase by interacting with TERT. PML-IV functions as a negative regulator of telomerase by interacting with TERT. J. Cell. Sci. (0021-9533) 2009 imex curation Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu Accepted 2013-SEP-23 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2009 imex curation PML-IV functions as a negative regulator of telomerase by interacting with TERT. PML-IV functions as a negative regulator of telomerase by interacting with TERT. J. Cell. Sci. (0021-9533) 2009 imex curation Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu Accepted 2013-SEP-23 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2009 imex curation PML-IV functions as a negative regulator of telomerase by interacting with TERT. PML-IV functions as a negative regulator of telomerase by interacting with TERT. J. Cell. Sci. (0021-9533) 2009 imex curation Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction Origin of TERT inferred from sequence length jso678@skku.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging anti-tag immunocytochemistry anti-tag immunocytochemistry Oh W., Ghim J., Lee E.W., Yang M.R., Kim E.T., Ahn J.H., Song J. Cardiac - Interactions involving cardiac related proteins This experiment relates to a negative interaction I did not merge EBI-8764945 and EBI-8763377 as Fig 2C positive data comprised EBI-8763377 data only. Instead I moved all other experiments (including EBI-8764945) to a separate experiment EBI-8766903. Is this ok? Origin of TERT inferred from sequence length jso678@skku.edu Accepted 2013-SEP-23 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Cell. Sci. (0021-9533) 2009 imex curation pml_human Protein PML PML MYL RNF71 TRIM19 Tripartite motif-containing protein 19 RING finger protein 71 Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS DIP protein P29590 original sequence version: 188 DIP protein P29590 original sequence version: 195 DIP protein P29590-1 original sequence version: 187 D50968A977E34287 p29590-5 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-IV PML-X TRIM19zeta Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNESGFSWGYPHPFLI DIP protein P29590-5 original sequence version: 188 DIP protein P29590-5 original sequence version: 195 85FBAEC9F162C8E0 tert_human Telomerase reverse transcriptase TERT EST2 TCS1 TRT Telomerase catalytic subunit HEST2 Telomerase-associated protein 2 protein protein human Homo sapiens Human MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPAAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGARRRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGNHAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSPWQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVNMDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFLLVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKLPGTTLTALEAAANPALPSDFKTILD DIP protein O14746 original sequence version: 129 DIP protein O14746 original sequence version: 95 DIP protein O14746 original sequence version: 125 DIP protein O14746 original sequence version: 121 DIP protein O14746-1 original sequence version: 110 94E35469C4CA33A0 pml-tert-1 5 9 anti-tag immunocytochemistry anti-tag immunocytochemistry immunocytochemistry immunocytochemistry immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Which one should be used? anti-tag immunocytochemistry or antibody detection? They seem both correct! 7 unspecified role unspecified role neutral component neutral component fixed cell fixed cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Figure 1C IFN-alpha pml5-tert-6 3 8 unspecified role unspecified role bait bait cell lysate cell lysate flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2 B pml-tert-12 2 8 unspecified role unspecified role bait bait cell lysate cell lysate binding region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3 E pml-tert-13 1 8 anti-tag immunocytochemistry anti-tag immunocytochemistry immunocytochemistry immunocytochemistry anti-tag immunocytochemistry anti-tag immunocytochemistry unspecified role unspecified role neutral component neutral component fixed cell fixed cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 anti-tag immunocytochemistry anti-tag immunocytochemistry immunocytochemistry immunocytochemistry anti-tag immunocytochemistry anti-tag immunocytochemistry unspecified role unspecified role neutral component neutral component fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Figures S4 C pml-tert-1 4 7 unspecified role unspecified role prey prey cell lysate cell lysate 9 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 1 D IFN-alpha pml-tert-14 6 8 unspecified role unspecified role neutral component neutral component fixed cell fixed cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Figure 2C pml-tert-15 1 8 anti-tag immunocytochemistry anti-tag immunocytochemistry immunocytochemistry immunocytochemistry anti-tag immunocytochemistry anti-tag immunocytochemistry unspecified role unspecified role neutral component neutral component fixed cell fixed cell flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 anti-tag immunocytochemistry anti-tag immunocytochemistry immunocytochemistry immunocytochemistry anti-tag immunocytochemistry anti-tag immunocytochemistry unspecified role unspecified role neutral component neutral component fixed cell fixed cell binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization Figures 5D tert-pml 2 9 unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey cell lysate cell lysate myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 51 55 52 56 physical association physical association Figures 5C tert-pml-1 2 9 unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey cell lysate cell lysate flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figures 5B