IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Three-dimensional structure of CaV3.1: comparison with the cardiac L-type voltage-gated calcium channel monomer architecture. Three-dimensional structure of CaV3.1: comparison with the cardiac L-type voltage-gated calcium channel monomer architecture. J. Biol. Chem. (0021-9258) 2009 imex curation Walsh C.P., Davies A., Butcher A.J., Dolphin A.C., Kitmitto A. Cardiac - Interactions involving cardiac related proteins ashraf.kitmitto@manchester.ac.uk 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Walsh C.P., Davies A., Butcher A.J., Dolphin A.C., Kitmitto A. Cardiac - Interactions involving cardiac related proteins Purification of the DHPR receptor is achieved with a well-established chromatographic method used in many other previous studies. For this reason, although some of the components of the purified receptor are only identified by MW, (as there are no specific antibodies against them) I accept this method of identification as valid. alpha 2 and delta domains shown in Fig 4A are known from previous studies to be the N-terminal and C-terminal respectively proteolytic products of the same polypeptide chain that are linked with a disulfide bond together to form the alpha2/delta domain within the DHPR channel. In bovine there is a Uniprot ID for the alpha2/delta domain, but not separate PRO IDs for each of alpha 2 and delta domains. Therefore, I added twice the alpha2/delta Uniprot ID having N-terminal and C-terminal features to indicate alpha 2 and delta domains respectively. ashraf.kitmitto@manchester.ac.uk. ashraf.kitmitto@manchester.ac.uk Accepted 2014-AUG-19 by ORCHARD J. Biol. Chem. (0021-9258) 2009 imex curation Three-dimensional structure of CaV3.1: comparison with the cardiac L-type voltage-gated calcium channel monomer architecture. Three-dimensional structure of CaV3.1: comparison with the cardiac L-type voltage-gated calcium channel monomer architecture. J. Biol. Chem. (0021-9258) 2009 imex curation Walsh C.P., Davies A., Butcher A.J., Dolphin A.C., Kitmitto A. Cardiac - Interactions involving cardiac related proteins ashraf.kitmitto@manchester.ac.uk 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions bovin-heart Bos taurus heast heart Heart [Cardiac; Cardiac tissue] affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom weight silver stain molecular weight estimation by silver staining weight silver stain Walsh C.P., Davies A., Butcher A.J., Dolphin A.C., Kitmitto A. Cardiac - Interactions involving cardiac related proteins I have added the comment about the purification method that was already added in PMID: 12946355. Purification of the DHPR receptor is achieved from bovine heart sarcolemmal membranes using a series of chromatographic columns. ashraf.kitmitto@manchester.ac.uk. ashraf.kitmitto@manchester.ac.uk Accepted 2014-AUG-19 by ORCHARD J. Biol. Chem. (0021-9258) 2009 imex curation cacb2_bovin Voltage-dependent L-type calcium channel subunit beta-2 CACNB2 Calcium channel voltage-dependent subunit beta 2 protein protein bovin Bos taurus (Bovine) Bovine MQCCGLVHRRRARVSYGSADSYTSRPSDSDVSLEEDREAVRREAERQAQAQLEKAKTKPVAFAVRTNVSYSAAHEDDVPVPGMAISFEAKDFLHVKEKFNNDWWIGRLVKEGCEIGFIPSPVKLENMRLQHEQRAKQGKFYSSKSGGNSSSSLGDIVPSSRKSTPPSSAIDIDATGLDAEDNDIPANHRSPKPSANSVTSPHSKEKRMPFFKKTEHTPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFEGRISITRVTADISLAKRSVLNNPSKHAIIERSNTRSSLAEVQSEIERIFELARTLQLVVLDADTINHPAQLSKTSLAPIIVYVKISSPKVLQRLIKSRGKSQAKHLNVQMVAADKLAQCPPELFDVILDENQLEDACEHLADYLEAYWKATHPPSSSLPNPLLSRTLATSTLPVSPTLASNSQGSQGDQRTDRGAPGRSASQAEEEHCPEPVKKAQHRSSTQHHNHRSGTSRGLSRQETLDSETQESRDSAYAEPKEEYSHEHADHYAPHRDHNHREEPHGGGEHRHREPRHRSRDPDREQDHNESNKQRSRHKSKDRYCDKDGEGLSRRRNEAADWNRDVYIRQ 217E10D1FA91B314 f1n7f9_bovin CACNA2D1 protein protein bovin Bos taurus (Bovine) Bovine MAAGCLLALTLTLFQSLLIGPSSEEPFPSAVTIKSWVDKMQEDLVTLAKTASGVNQLVDIYEKYQDLYTVEPNNARQLVEIAARDIEKLLSNRSKALVRLALEAEKVQAAHQWREDFASNEVVYYNAKDDLDPEKNDSEPGSQRIKPVFIDDANFGRQISYQHAAVHIPTDIYEGSTIVLNELNWTSALDEVFKKNREEDPSLLWQVFGSATGLARYYPASPWVDNSRTPNKIDLYDVRRRPWYIQGAASPKDMLILVDVSGSVSGLTLKLIRTSVSEMLETLSDDDFVNVASFNSNAQDVSCFQHLVQANVRNKKVLKDAVNNITAKGITDYKKGFSFAFEQLLNYNVSRANCNKIIMLFTDGGEERAQEIFTKYNKDKKVRVFTFSVGQHNYDRGPIQWMACENKGYYYEIPSIGAIRINTQEYLDVLGRPMVLAGDKAKQVQWTNVYLDALELGLVITGTLPVFNITGQNENKTNLKNQLILGVMGVDVSLEDIKRLTPRFTLCPNGYYFAIDPNGYVLLHPNLQPKPIGLGIPTINLRKRRPNVQNPKSQEPVTLDFLDAELENDIKVEIRNKMIDGESGEKTFRTLVKSQDERYIDKGNRTYTWTPVNGTDYSLALVLPTYSFYYIKAKIEETITQARYSETLKPDNFEESGYTFIAPRDYCNDLKISDNNTEFLLNFNEFIDRKTPNNPSCNTDLINRVLLDAGFTNELVQNYWSKQKNIKGVKARFVVTDGGITRVYPKEAGENWQENPETYEDSFYKRSLDNDNYVFTAPYFNKSGPGAYESGIMVSKAVEIYIQGKLLKPAVVGIKIDLNSWIENFTKTSIRDPCAGPVCDCKRNSDVMDCVILDDGGFLLMANHDDYTNQIGRFFGEIDPSLMRHLVNISVYAYNKSYDYQSVCEPGAAPKQGAGHRSAYVPSIADVLHIGWWATAAAWSILQQFLLSLTFPRLLEAVEMEDDDFTASLSKQSCITEQTQYFFDNDSKSFSGVLDCGNCSRIFHVEKLMNTNLIFIMVESKGTCPCDTRLLIQAEQTSDGPDPCDMVKQPRYRKGPDVCFDNNALEDYTDCGGVSGLNPSLWSIIGIQCVLLWLLSGSRHHQL F677480F592BAC36 f1n5t3_bovin Voltage-dependent L-type calcium channel subunit alpha CACNA1C protein protein bovin Bos taurus (Bovine) Bovine ERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNSERPPKCQKYLYFISLSVSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMVRPSLQTTLSASVTPLTSPLPPPTPGVSEPGPDACHPPVSAMRLACLVCELSNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL 5169855A14156AC9 cacna1c-cacna2d1-1 1 4 unspecified role unspecified role neutral component neutral component purified purified n-terminal binding region binding-associated region binding region feature prediction feature prediction undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined bovin-heart Bos taurus heast heart Heart [Cardiac; Cardiac tissue] 3 unspecified role unspecified role neutral component neutral component purified purified bovin-heart Bos taurus heast heart Heart [Cardiac; Cardiac tissue] 4 unspecified role unspecified role neutral component neutral component purified purified c-terminal binding region binding-associated region binding region feature prediction feature prediction undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined bovin-heart Bos taurus heast heart Heart [Cardiac; Cardiac tissue] 5 unspecified role unspecified role neutral component neutral component purified purified bovin-heart Bos taurus heast heart Heart [Cardiac; Cardiac tissue] physical association physical association Fig. 1 A, B, Fig. 5 cacna1c-cacna2d1-2 2 3 unspecified role unspecified role neutral component neutral component purified purified 4 unspecified role unspecified role neutral component neutral component purified purified n-terminal binding region binding-associated region binding region feature prediction feature prediction undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component purified purified c-terminal binding region binding-associated region binding region feature prediction feature prediction undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component purified purified physical association physical association Fig. 4 A, B