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  <entry>
    <source releaseDate="2022-02-04">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
      <bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="14681455" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
      </bibref>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0469" refType="identity" refTypeAc="MI:0356"/>
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        <secondaryRef db="pubmed" dbAc="MI:0446" id="19850723" refType="method reference" refTypeAc="MI:0357"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Interaction of calcium/calmodulin-dependent protein kinase IIdeltaC with sorcin indirectly modulates ryanodine receptor function in cardiac myocytes.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="17707398" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-10768415" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-24152" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Interaction of calcium/calmodulin-dependent protein kinase IIdeltaC with sorcin indirectly modulates ryanodine receptor function in cardiac myocytes.</attribute>
            <attribute name="journal" nameAc="MI:0885">Journal of molecular and cellular cardiology</attribute>
            <attribute name="publication year" nameAc="MI:0886">2007</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Anthony DF., Beattie J., Paul A., Currie S.</attribute>
            <attribute name="dataset" nameAc="MI:0875">Cardiac - Interactions involving cardiac related proteins</attribute>
            <attribute name="comment" nameAc="MI:0612">Interactions involving  the CaMKIIdeltaC construct have not been included as the origin of this construct cannot be traced and the corresponding author did not reply to a request for information.</attribute>
            <attribute name="remark-internal">email sent 24/08/15; not expecting a reply so will have to delete all expts except for the accepted one.&#xd;
&#xd;
The donor of the CaMKIIdeltaC construct  is "Prof R. Colbran"- who is mentioned on several of the "Rat" papers but who is not mentioned in context with any particular species of CaMKIIdeltaC; much of his work is Human associated,  but not  using  CaMKIIdeltaC.&#xd;
The isoform identification was not easy - UniProt lists several isoforms but does not use the terms "A, B, C" etc... and NCBI  Gene page does not even mention isoforms.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">susan.currie@strath.ac.uk</attribute>
            <attribute name="author-announcement">21-10-2015: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="17707398" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-24152" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>electrophoresis</shortLabel>
            <fullName>electrophoretic mobility-based method</fullName>
            <alias type="synonym" typeAc="MI:1041">electrophoresis</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0982" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5527527" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-973" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Anthony DF., Beattie J., Paul A., Currie S.</attribute>
          <attribute name="dataset" nameAc="MI:0875">Cardiac - Interactions involving cardiac related proteins</attribute>
          <attribute name="comment" nameAc="MI:0612">Interactions involving  the CaMKIIdeltaC construct have not been included as the origin of this construct cannot be traced and the corresponding author did not reply to a request for information.</attribute>
          <attribute name="remark-internal">email sent 24/08/15; not expecting a reply so will have to delete all expts except for the accepted one.&#xd;
&#xd;
The donor of the CaMKIIdeltaC construct  is "Prof R. Colbran"- who is mentioned on several of the "Rat" papers but who is not mentioned in context with any particular species of CaMKIIdeltaC; much of his work is Human associated,  but not  using  CaMKIIdeltaC.&#xd;
The isoform identification was not easy - UniProt lists several isoforms but does not use the terms "A, B, C" etc... and NCBI  Gene page does not even mention isoforms.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">susan.currie@strath.ac.uk</attribute>
          <attribute name="accepted">Accepted 2015-SEP-22 AT 11:46 BST AT 11:46 BST by ORCHARD</attribute>
          <attribute name="journal" nameAc="MI:0885">Journal of molecular and cellular cardiology</attribute>
          <attribute name="publication year" nameAc="MI:0886">2007</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959">imex curation</attribute>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
          <attribute name="correction comment"/>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>sorcn_crigr</shortLabel>
          <fullName>Sorcin</fullName>
          <alias type="gene name synonym" typeAc="MI:0302">22 kDa protein</alias>
          <alias type="gene name synonym" typeAc="MI:0302">CP-22</alias>
          <alias type="gene name synonym" typeAc="MI:0302">V19</alias>
          <alias type="gene name" typeAc="MI:0301">SRI</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P05044" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-10816377" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1GJY"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011992"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR018247"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR002048"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005509" version="SP_121"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016020" version="SP_121"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0033017" version="SP_121"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
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            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="10029">
          <names>
            <shortLabel>crigr</shortLabel>
            <fullName>Cricetulus griseus (Chinese hamster)</fullName>
            <alias type="synonym" typeAc="MI:1041">Cricetulus barabensis griseus</alias>
            <alias type="synonym" typeAc="MI:1041">Chinese hamster</alias>
          </names>
        </organism>
        <sequence>MAYPGHPGAGGGYYPGGYGGAPGGPSFPGQTQDPLYGYFASVAGQDGQIDADELQRCLTQSGIAGGYKPFNLETCRLMVSMLDRDMSGTMGFNEFKELWAVLNGWRQHFISFDSDRSGTVDPQELQKALTTMGFRLNPQTVNSIAKRYSTSGKITFDDYIACCVKLRALTDSFRRRDSAQQGMVNFSYDDFIQCVMTV</sequence>
        <attributeList>
          <attribute name="crc64">DA2AF8A9707561C5</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-24152-5">
        <names>
          <shortLabel>sri-10</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-10816472" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-24152-5" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>prey</shortLabel>
                  <fullName>prey</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0498" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-58" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="2"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
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            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">3C</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>