bhf-ucl Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol. (0021-9525) 2007 imex curation Ball S.G., Shuttleworth C.A., Kielty C.M. Cardiac - Interactions involving cardiac related proteins adrian.shuttleworth@manchester.ac.uk,cay.kielty@manchester.ac.uk 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink antibody detection identification by antibody antibody detection Ball S.G., Shuttleworth C.A., Kielty C.M. Cardiac - Interactions involving cardiac related proteins After the cross-linking treatment, the receptors were immunoprecipitated using the corresponding antibodies. The growth factor association was detected by immunoblot analysis using the corresponding antibodies. MSCs cells were also pretreated with either anti-PDGFR alpha or anti-PDGFR beta. After this treatment the binding of the receptors to the corresponding growth factors was significantly reduced. adrian.shuttleworth@manchester.ac.uk cay.kielty@manchester.ac.uk adrian.shuttleworth@manchester.ac.uk,cay.kielty@manchester.ac.uk Accepted 2014-JAN-28 by ORCHARD J. Cell Biol. (0021-9525) 2007 imex curation pgfrb_human Platelet-derived growth factor receptor beta CD140 antigen-like family member B PDGFRB Beta-type platelet-derived growth factor receptor PDGFR Platelet-derived growth factor receptor 1 Beta platelet-derived growth factor receptor PDGFR1 protein protein human Homo sapiens Human MRLPGAMPALALKGELLLLSLLLLLEPQISQGLVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQKKPRYEIRWKVIESVSSDGHEYIYVDPMQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL DIP protein P09619 original sequence version: 176 DIP protein P09619 original sequence version: 185 DIP protein P09619 original sequence version: 114 DIP protein P09619 original sequence version: 162 DIP protein P09619 original sequence version: 150 DIP protein P09619 original sequence version: 165 DIP protein P09619 original sequence version: 161 038C15E531D6E89D p15692-4 Vascular endothelial growth factor A VEGF VEGFA VEGF Vascular permeability factor protein protein human Homo sapiens Human MNFLLSWVHWSLALLLYLHHAKWSQAAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR DIP protein P15692-4 original sequence version: 150 CCE57097DD3779BD pdgfb_human Platelet-derived growth factor subunit B Platelet-derived growth factor B chain Platelet-derived growth factor beta polypeptide PDGF-2 Proto-oncogene c-Sis PDGFB PDGF2 SIS protein protein human Homo sapiens Human MNRCWALFLSLCCYLRLVSAEGDPIPEELYEMLSDHSIRSFDDLQRLLHGDPGEEDGAELDLNMTRSHSGGELESLARGRRSLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVTRSPGGSQEQRAKTPQTRVTIRTVRVRRPPKGKHRKFKHTHDKTALKETLGA DIP protein P01127-1 original sequence version: 170 DIP protein P01127 original sequence version: 145 DIP protein P01127 original sequence version: 162 9F9A3474CE203C0B pgfra_human Platelet-derived growth factor receptor alpha PDGFRA CD140 antigen-like family member A CD140a antigen Alpha-type platelet-derived growth factor receptor Alpha platelet-derived growth factor receptor Platelet-derived growth factor receptor 2 RHEPDGFRA PDGFR2 Platelet-derived growth factor alpha receptor protein protein human Homo sapiens Human MGTSHPAFLVLGCLLTGLSLILCQLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATREVKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIENLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSILDLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHSRDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVVIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFPRDGLVLGRVLGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGPHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFLSHHPEKPKKELDIFGLNPADESTRSYVILSFENNGDYMDMKQADTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEVKNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQGKIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIFSLGGTPYPGMMVDSTFYNKIKSGYRMAKPDHATSEVYEIMVKCWNSEPEKRPSFYHLSEIVENLLPGQYKKSYEKIHLDFLKSDHPAVARMRVDSDNAYIGVTYKNEEDKLKDWEGGLDEQRLSADSGYIIPLPDIDPVPEEEDLGKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIDMMDDIGIDSSDLVEDSFL 5E3FB9940ACD1BE8 pdgfb-pdgfra-6 1 4 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 5 unspecified role unspecified role neutral component neutral component human-msc Homo sapiens mesenchymal stem cells msc mesenchymal stem cells physical association physical association Fig. 8 pdgfb-pdgfrb-2 1 4 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 2 unspecified role unspecified role neutral component neutral component human-msc Homo sapiens mesenchymal stem cells msc mesenchymal stem cells 3 unspecified role unspecified role neutral component neutral component spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association Fig. 8