IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural basis for modulation of Kv4 K+ channels by auxiliary KChIP subunits. Structural basis for modulation of Kv4 K+ channels by auxiliary KChIP subunits. Nat. Neurosci. (1097-6256) 2007 imex curation Wang H., Yan Y., Liu Q., Huang Y., Shen Y., Chen L., Chen Y., Yang Q., Hao Q., Wang K., Chai J. Cardiac - Interactions involving cardiac related proteins chaijijie@nibs.ac.cn 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang H., Yan Y., Liu Q., Huang Y., Shen Y., Chen L., Chen Y., Yang Q., Hao Q., Wang K., Chai J. Cardiac - Interactions involving cardiac related proteins chaijijie@nibs.ac.cn Accepted 2014-NOV-26 by ORCHARD Nat. Neurosci. (1097-6256) 2007 imex curation Structural basis for modulation of Kv4 K+ channels by auxiliary KChIP subunits. Structural basis for modulation of Kv4 K+ channels by auxiliary KChIP subunits. Nat. Neurosci. (1097-6256) 2007 imex curation Wang H., Yan Y., Liu Q., Huang Y., Shen Y., Chen L., Chen Y., Yang Q., Hao Q., Wang K., Chai J. Cardiac - Interactions involving cardiac related proteins chaijijie@nibs.ac.cn 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Wang H., Yan Y., Liu Q., Huang Y., Shen Y., Chen L., Chen Y., Yang Q., Hao Q., Wang K., Chai J. Cardiac - Interactions involving cardiac related proteins chaijijie@nibs.ac.cn Accepted 2014-NOV-26 by ORCHARD Nat. Neurosci. (1097-6256) 2007 imex curation kcnd3_human Potassium voltage-gated channel subfamily D member 3 KCND3 Voltage-gated potassium channel subunit Kv4.3 protein protein human Homo sapiens Human MAAGVAAWLPFARAAAIGWMPVANCPMPLAPADKNKRQDELIVLNVSGRRFQTWRTTLERYPDTLLGSTEKEFFFNEDTKEYFFDRDPEVFRCVLNFYRTGKLHYPRYECISAYDDELAFYGILPEIIGDCCYEEYKDRKRENAERLMDDNDSENNQESMPSLSFRQTMWRAFENPHTSTLALVFYYVTGFFIAVSVITNVVETVPCGTVPGSKELPCGERYSVAFFCLDTACVMIFTVEYLLRLFAAPSRYRFIRSVMSIIDVVAIMPYYIGLVMTNNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGSSASKFTSIPASFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRRAQKKARLARIRVAKTGSSNAYLHSKRNGLLNEALELTGTPEEEHMGKTTSLIESQHHHLLHCLEKTTGLSYLVDDPLLSVRTSTIKNHEFIDEQMFEQNCMESSMQNYPSTRSPSLSSHPGLTTTCCSRRSKKTTHLPNSNLPATRLRSMQELSTIHIQGSEQPSLTTSRSSLNLKADDGLRPNCKTSQITTAIISIPTPPALTPEGESRPPPASPGPNTNIPSIASNVVKVSAL ADD1402A97204764 kcip1_human Kv channel-interacting protein 1 KCNIP1 KCHIP1 VABP A-type potassium channel modulatory protein 1 Potassium channel-interacting protein 1 Vesicle APC-binding protein protein protein human Homo sapiens Human MGAVMGTFSSLQTKQRRPSKDIAWWYYQYQRDKIEDELEMTMVCHRPEGLEQLEAQTNFTKRELQVLYRGFKNECPSGVVNEDTFKQIYAQFFPHGDASTYAHYLFNAFDTTQTGSVKFEDFVTALSILLRGTVHEKLRWTFNLYDINKDGYINKEEMMDIVKAIYDMMGKYTYPVLKEDTPRQHVDVFFQKMDKNKDGIVTLDEFLESCQEDDNIMRSLQLFQNVM DIP protein Q9NZI2 original sequence version: 48 D39DD5F8EA13B0FD kcnd3-kcnip1 1 3 unspecified role unspecified role neutral component neutral component n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 7 physical association physical association Fig. 1 Q9NZI2 increases activity of Q9UK17 3.2 Angstrom R = 0.26 Rwork = 0.26 Rfree = 0.31 kcnd3-kcnip1-1 2 3 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9NZI2:p.Val134Glu mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain V E Wed May 02 18:19:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val134glu' Tue Jun 30 18:04:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val134Glu' n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 15 direct interaction direct interaction Fig. 3b kcnip1-kcnd3 2 4 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9NZI2:p.Val134Glu mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain V E Wed May 02 20:24:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val134glu' Tue Jun 30 15:37:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val134Glu' ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role prey prey n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9UK17:p.[Trp8Glu;Pro10Glu;Ala15Glu] mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain W E certain certain sequence position certain certain certain sequence position certain P E certain certain sequence position certain certain certain sequence position certain A E Wed May 02 18:13:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp8glu,pro10glu,ala15glu' Tue Jun 30 16:35:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp8Glu;Pro10Glu;Ala15Glu]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3b