bhf-ucl Role of the kinesin-2 family protein, KIF3, during mitosis. Role of the kinesin-2 family protein, KIF3, during mitosis. J. Biol. Chem. (0021-9258) 2006 imex curation Haraguchi K., Hayashi T., Jimbo T., Yamamoto T., Akiyama T. Cardiac - Interactions involving cardiac related proteins The length of the KIP3A protein showed in Fig. 3 is 702 aa but the current one in UniProt is 699 aa. After aligning both sequences (the current one and the one found in History in 2006) we found that the 702 aa long protein has an insertion of three extra amino acids between positions 408 and 409 of the current sequence (699 aa long protein) GFP-KIF3A, GFP-KIF3B and GFP-KAP3 recombinant proteins in Fig. 3 are human. Confirmed by author. akiyama@iam.u-tokyo.ac.jp 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Haraguchi K., Hayashi T., Jimbo T., Yamamoto T., Akiyama T. Cardiac - Interactions involving cardiac related proteins Lysates were prepared from HeLa cells arrested in mitosis by nocodazole treatment. The length of the KIP3A protein showed in Fig. 3 is 702 aa but the current one in UniProt is 699 aa. After aligning both sequences (the current one and the one found in History in 2006) we found that the 702 aa long protein has an insertion of three extra amino acids between positions 408 and 409 of the current sequence (699 aa long protein) akiyama@iam.u-tokyo.ac.jp Accepted 2014-JUL-08 by ORCHARD J. Biol. Chem. (0021-9258) 2006 imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Role of the kinesin-2 family protein, KIF3, during mitosis. Role of the kinesin-2 family protein, KIF3, during mitosis. J. Biol. Chem. (0021-9258) 2006 imex curation Haraguchi K., Hayashi T., Jimbo T., Yamamoto T., Akiyama T. Cardiac - Interactions involving cardiac related proteins The length of the KIP3A protein showed in Fig. 3 is 702 aa but the current one in UniProt is 699 aa. After aligning both sequences (the current one and the one found in History in 2006) we found that the 702 aa long protein has an insertion of three extra amino acids between positions 408 and 409 of the current sequence (699 aa long protein) GFP-KIF3A, GFP-KIF3B and GFP-KAP3 recombinant proteins in Fig. 3 are human. Confirmed by author. akiyama@iam.u-tokyo.ac.jp 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Haraguchi K., Hayashi T., Jimbo T., Yamamoto T., Akiyama T. Cardiac - Interactions involving cardiac related proteins The length of the KIP3A protein showed in Fig. 3 is 702 aa but the current one in UniProt is 699 aa. After aligning both sequences (the current one and the one found in History in 2006) we found that the 702 aa long protein has an insertion of three extra amino acids between positions 408 and 409 of the current sequence (699 aa long protein) akiyama@iam.u-tokyo.ac.jp Accepted 2014-JUL-08 by ORCHARD J. Biol. Chem. (0021-9258) 2006 imex curation Role of the kinesin-2 family protein, KIF3, during mitosis. Role of the kinesin-2 family protein, KIF3, during mitosis. J. Biol. Chem. (0021-9258) 2006 imex curation Haraguchi K., Hayashi T., Jimbo T., Yamamoto T., Akiyama T. Cardiac - Interactions involving cardiac related proteins The length of the KIP3A protein showed in Fig. 3 is 702 aa but the current one in UniProt is 699 aa. After aligning both sequences (the current one and the one found in History in 2006) we found that the 702 aa long protein has an insertion of three extra amino acids between positions 408 and 409 of the current sequence (699 aa long protein) GFP-KIF3A, GFP-KIF3B and GFP-KAP3 recombinant proteins in Fig. 3 are human. Confirmed by author. akiyama@iam.u-tokyo.ac.jp 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Haraguchi K., Hayashi T., Jimbo T., Yamamoto T., Akiyama T. Cardiac - Interactions involving cardiac related proteins The length of the KIP3A protein showed in Fig. 3 is 702 aa but the current one in UniProt is 699 aa. After aligning both sequences (the current one and the one found in History in 2006) we found that the 702 aa long protein has an insertion of three extra amino acids between positions 408 and 409 of the current sequence (699 aa long protein) akiyama@iam.u-tokyo.ac.jp Accepted 2014-JUL-08 by ORCHARD J. Biol. Chem. (0021-9258) 2006 imex curation kif3b_human Kinesin-like protein KIF3B KIF3B KIAA0359 HH0048 Microtubule plus end-directed kinesin motor 3B protein protein human Homo sapiens Human MSKLKSSESVRVVVRCRPMNGKEKAASYDKVVDVDVKLGQVSVKNPKGTAHEMPKTFTFDAVYDWNAKQFELYDETFRPLVDSVLQGFNGTIFAYGQTGTGKTYTMEGIRGDPEKRGVIPNSFDHIFTHISRSQNQQYLVRASYLEIYQEEIRDLLSKDQTKRLELKERPDTGVYVKDLSSFVTKSVKEIEHVMNVGNQNRSVGATNMNEHSSRSHAIFVITIECSEVGLDGENHIRVGKLNLVDLAGSERQAKTGAQGERLKEATKINLSLSALGNVISALVDGKSTHIPYRDSKLTRLLQDSLGGNAKTVMVANVGPASYNVEETLTTLRYANRAKNIKNKPRVNEDPKDALLREFQEEIARLKAQLEKRSIGRRKRREKRREGGGSGGGGEEEEEEGEEGEEEGDDKDDYWREQQEKLEIEKRAIVEDHSLVAEEKMRLLKEKEKKMEDLRREKDAAEMLGAKIKAMESKLLVGGKNIVDHTNEQQKILEQKRQEIAEQKRREREIQQQMESRDEETLELKETYSSLQQEVDIKTKKLKKLFSKLQAVKAEIHDLQEEHIKERQELEQTQNELTRELKLKHLIIENFIPLEEKSKIMNRAFFDEEEDHWKLHPITRLENQQMMKRPVSAVGYKRPLSQHARMSMMIRPEARYRAENIVLLELDMPSRTTRDYEGPAIAPKVQAALDAALQDEDEIQVDASSFESTANKKSKARPKSGRKSGSSSSSSGTPASQLYPQSRGLVPK 97FA4573AFA87023 kif3a_human Kinesin-like protein KIF3A KIF3A KIF3 Microtubule plus end-directed kinesin motor 3A protein protein human Homo sapiens Human MPINKSEKPESCDNVKVVVRCRPLNEREKSMCYKQAVSVDEMRGTITVHKTDSSNEPPKTFTFDTVFGPESKQLDVYNLTARPIIDSVLEGYNGTIFAYGQTGTGKTFTMEGVRAIPELRGIIPNSFAHIFGHIAKAEGDTRFLVRVSYLEIYNEEVRDLLGKDQTQRLEVKERPDVGVYIKDLSAYVVNNADDMDRIMTLGHKNRSVGATNMNEHSSRSHAIFTITIECSEKGIDGNMHVRMGKLHLVDLAGSERQAKTGATGQRLKEATKINLSLSTLGNVISALVDGKSTHVPYRNSKLTRLLQDSLGGNSKTMMCANIGPADYNYDETISTLRYANRAKNIKNKARINEDPKDALLRQFQKEIEELKKKLEEGEEISGSDISGSEEDDDEEGEVGEDGEKRKKRRGKKKVSPDKMIEMQAKIDEERKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVPDKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKTGRRKRSAKPETVIDSLLQ DIP protein Q9Y496 original sequence version: 79 A0BF5BB1F699DF12 kifa3_human Kinesin-associated protein 3 Smg GDS-associated protein KIFAP3 SMAP KIF3AP protein protein human Homo sapiens Human MQGEDARYLKRKVKGGNIDVHPSEKALIVHYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSLARKVVEECKLIHPSKLNEVEQLLYYLQNRRDSLSGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIELLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSQFHGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDPENQTLRKDYEKTFKKYQGLVVKQEQLLRVALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLVKMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNDNYKQIAMCVLYHISMDDRFKSMFAYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKFKDPLLMKMIRNISQHDGPTKNLFIDYVGDLAAQISNDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPYLKDKLKPGAAEDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIKETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQMDESEQYLYGDDRIEPYIHEGDILERPDLFYNSDGLIASEGAISPDFFNDYHLQNGDVVGQHSFPGSLGMDGFGQPVGILGRPATAYGFRPDEPYYYGYGS 1887A75D811DF56A kifap3-kif3a_kif3b-1 1 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig. 2 kif3a-kifap3-1 4 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4 KAP3 and KIF3A colocalized in: the spindle of microtubules during prometaphase the centrosome during metaphase to telophase kif3a-kifap3-4 3 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association Fig. 3A kif3b-kifap3 3 5 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association Fig. 3A kifap3-kif3a-2 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association Fig. 3B kif3a-kif3b_human KIF3A:KIF3B KIF3 complex variant AB heterodimeric kinesin-2 motor complex KIF3 cytoplasmic motor protein complex KIF3 6 unspecified role unspecified role kif3b binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role kif3a binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 32 34 direct interaction direct interaction human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Cytoplasmic, kinesin-2 motor complex involved in tethering the chromosomes to the spindle pole and in chromosome movement. Microtubule-based anterograde translocator for membranous organelles. Exhibits plus end-directed microtubule sliding activity (in vitro). It is unclear if this dimer exists in vivo or whether it is always in complex with KAP3 (Q92845). Heterodimer created with intact-jami-dbscripts