bhf-ucl TIM-4 is the ligand for TIM-1, and the TIM-1-TIM-4 interaction regulates T cell proliferation. TIM-4 is the ligand for TIM-1, and the TIM-1-TIM-4 interaction regulates T cell proliferation. Nature immunology 2005 imex curation Meyers JH., Chakravarti S., Schlesinger D., Illes Z., Waldner H., Umetsu SE., Kenny J., Zheng XX., Umetsu DT., DeKruyff RH., Strom TB., Kuchroo VK. Cardiac - Interactions involving cardiac related proteins The authors have confirmed that TIM-4 and TIM-1 constructs are of Murine origin. vkuchroo@rics.bwh.harvard.edu 17-May-2018: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Meyers JH., Chakravarti S., Schlesinger D., Illes Z., Waldner H., Umetsu SE., Kenny J., Zheng XX., Umetsu DT., DeKruyff RH., Strom TB., Kuchroo VK. Cardiac - Interactions involving cardiac related proteins Fig 3a. Author states:"All immunoglobulin fusion proteins were made by Chimerigen, with fusing of the extracellular regions of the proteins of interest to a human IgG1 Fc tail". So I mapped to corresponding extracellular domains as per UniProt. Is this OK? Also, do I need to mention this in public field? e.g. comment or data-processing?. "Data not shown" as per author statement with regards to binding Havcr1 (Tim-1) IgV domain was not mapped as per instructions in manual, author states: "There was staining of TIM-4 transfectants with TIM-1–Ig fusion protein whether full-length TIM-1–Ig, containing the TIM-1 IgV and mucin domains, or a TIM-1–Ig containing only the IgV domain was used (Fig. 3a and data not shown)" Also, since I have created a binding domain (containg the extracellular domain only) in the x-Ig soluble fusion protein, should I create an extracellular domain only for the transfected protein and create a link between the two. Note that the transfected protein is full length, however, only the extracellular region 'should' be making the interaction. Please advise. Thanks. The authors have confirmed that TIM-4 and TIM-1 constructs are of Murine origin. vkuchroo@rics.bwh.harvard.edu Accepted 2018-MAY-01 AT 10:54 BST AT 10:54 BST by MGT Nature immunology 2005 Only protein-protein interactions imex curation imex curation q5qns5-2 Hepatitis A virus cellular receptor 1 homolog Kidney injury molecule 1 T cell immunoglobulin and mucin domain-containing protein 1 T cell membrane protein 1 T-cell immunoglobulin mucin receptor 1 Havcr1 Kim1 Tim1 Timd1 protein protein mouse Mus musculus Mouse MNQIQVFISGLILLLPGAVDSYVEVKGVVGHPVTLPCTYSTYRGITTTCWGRGQCPSSACQNTLIWTNGHRVTYQKSSRYNLKGHISEGDVSLTIENSVESDSGLYCCRVEIPGWFNDQKVTFSLQVKPEIPTRPPTRPTTTRPTATGRPTTISTRSTHVPTSIRVSTSTPPTSTHTWTHKPDWNGTVTSSGDTWSNHTEAIPPGKPQKNPTKGFYVGICIAALLLLLLVSTVAITRYILMKRKSASLSVVAFRVSKIEALQNAAVVHSRAEDNIYIVEDRP A05455445ED0A5C9 timd4_mouse T-cell immunoglobulin and mucin domain-containing protein 4 Timd4 Tim4 Spleen, mucin-containing, knockout of lymphotoxin protein T-cell immunoglobulin mucin receptor 4 T-cell membrane protein 4 protein protein mouse Mus musculus Mouse MSKGLLLLWLVTELWWLYLTPAASEDTIIGFLGQPVTLPCHYLSWSQSRNSMCWGKGSCPNSKCNAELLRTDGTRIISRKSTKYTLLGKVQFGEVSLTISNTNRGDSGVYCCRIEVPGWFNDVKKNVRLELRRATTTKKPTTTTRPTTTPYVTTTTPELLPTTVMTTSVLPTTTPPQTLATTAFSTAVTTCPSTTPGSFSQETTKGSAFTTESETLPASNHSQRSMMTISTDIAVLRPTGSNPGILPSTSQLTTQKTTLTTSESLQKTTKSHQINSRQTILIIACCVGFVLMVLLFLAFLLRGKVTGANCLQRHKRPDNTEDSDSVLNDMSHGRDDEDGIFTL D2D5539A7CDB8290 havr1_mouse Hepatitis A virus cellular receptor 1 homolog Kidney injury molecule 1 T cell immunoglobulin and mucin domain-containing protein 1 T cell membrane protein 1 T-cell immunoglobulin mucin receptor 1 Havcr1 Kim1 Tim1 Timd1 protein protein mouse Mus musculus Mouse MNQIQVFISGLILLLPGAVDSYVEVKGVVGHPVTLPCTYSTYRGITTTCWGRGQCPSSACQNTLIWTNGHRVTYQKSSRYNLKGHISEGDVSLTIENSVESDSGLYCCRVEIPGWFNDQKVTFSLQVKPEIPTRPPTRPTTTRPTATGRPTTISTRSTHVPTSIRVSTSTPPTSTHTWTHKPEPTTFCPHETTAEVTGIPSHTPTDWNGTVTSSGDTWSNHTEAIPPGKPQKNPTKGFYVGICIAALLLLLLVSTVAITRYILMKRKSASLSVVAFRVSKIEALQNAAVVHSRAEDNIYIVEDRP F995C93A9125E4FE timd4-havcr1-1 1 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ha tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminal_pdgfr_tm_domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 2 unspecified role unspecified role prey prey purified purified region Timd4 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Figure 3a second row far right The authors state that:-"There was staining of TIM-4 transfectants with TIM-1–Ig fusion protein whether full-length TIM-1–Ig, containing the TIM-1 IgV and mucin domains, or a TIM-1–Ig containing only the IgV domain was used". timd4-havcr1-2 1 3 unspecified role unspecified role prey prey purified purified c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd Havcr1 binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Figure 3a fourth row far left timd4-havcr1-3 1 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed ha tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminal_pdgfr_tm_domain tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 2 unspecified role unspecified role prey prey purified purified region Timd4 binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Figure 3b havcr1-timd4-4 1 3 unspecified role unspecified role prey prey purified purified c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd Havcr1 binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 4 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association Figure 3b havcr1-timd4-1 1 3 unspecified role unspecified role prey prey purified purified c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd Havcr1 binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 4 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes physical association physical association Figure 4A TIM-4–Ig stained T cells activated with anti-CD3 and anti-CD28, but did not stain unactivated T cells. havcr1-timd4-5 1 3 unspecified role unspecified role prey prey purified purified c_terminus human IgG1 Fc tail fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecd Havcr1 binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 4 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-spleen Mus musculus spleen spleen spleen physical association physical association Figure 4B splenocytes were stimulated with concanavalin A.