bhf-ucl Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol. Biol. Cell (1059-1524) 2004 imex curation Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins gerd.schmitz@klinik.uniregensburg.de 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-macrophage Human macrophages macrophage Macrophage anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins They use human monocytes derived macrophages gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Accepted 2014-AUG-15 by PPORRAS Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol. Biol. Cell (1059-1524) 2004 imex curation Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins gerd.schmitz@klinik.uniregensburg.de 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-e-ldl loaded macrophages Human-E-LDL loaded macrophages human-e-ldl loaded macrophages Human-E-LDL loaded macrophages anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins Macrophages were incubated with enzymatically modified low-density lipoprotein (E-LDL) gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Accepted 2014-AUG-15 by PPORRAS Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol. Biol. Cell (1059-1524) 2004 imex curation Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins gerd.schmitz@klinik.uniregensburg.de 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-macrophage Human macrophages macrophage Macrophage fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Accepted 2014-AUG-15 by PPORRAS Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol. Biol. Cell (1059-1524) 2004 imex curation Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins gerd.schmitz@klinik.uniregensburg.de 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hl_60 Homo sapiens acute myeloid leukemia cell line hl_60 human acute myeloid leukemia cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins The HL-60 cells were treated with PMA (phorbol 12-myristate 13-acetate) to induce macrophage differentiation gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Accepted 2014-AUG-15 by PPORRAS Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Association of ABCA1 with syntaxin 13 and flotillin-1 and enhanced phagocytosis in tangier cells. Mol. Biol. Cell (1059-1524) 2004 imex curation Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins gerd.schmitz@klinik.uniregensburg.de 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-e-ldl loaded macrophages Human-E-LDL loaded macrophages human-e-ldl loaded macrophages Human-E-LDL loaded macrophages density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Bared S.M., Buechler C., Boettcher A., Dayoub R., Sigruener A., Grandl M., Rudolph C., Dada A., Schmitz G. Cardiac - Interactions involving cardiac related proteins Human E-LDL loaded macrophages were solubilized in 1% Lubrol WX or 1% Triton X100 and fractionated by sucrose gradient centrifugation. gerd.schmitz@klinik.uni- regensburg.de gerd.schmitz@klinik.uniregensburg.de Accepted 2014-AUG-15 by PPORRAS Mol. Biol. Cell (1059-1524) 2004 Only protein-protein interactions imex curation stx12_human Syntaxin-12 STX12 protein protein human Homo sapiens Human MSYGPLDMYRNPGPSGPQLRDFSSIIQTCSGNIQRISQATAQIKNLMSQLGTKQDSSKLQENLQQLQHSTNQLAKETNELLKELGSLPLPLSTSEQRQQRLQKERLMNDFSAALNNFQAVQRRVSEKEKESIARARAGSRLSAEERQREEQLVSFDSHEEWNQMQSQEDEVAITEQDLELIKERETAIRQLEADILDVNQIFKDLAMMIHDQGDLIDSIEANVESSEVHVERATEQLQRAAYYQKKSRKKMCILVLVLSVIILILGLIIWLVYKTK DIP protein Q86Y82 original sequence version: 119 2338061572ED7DA1 abca1_human Phospholipid-transporting ATPase ABCA1 ATP-binding cassette transporter 1 Cholesterol efflux regulatory protein ABCA1 ABC1 CERP ATP-binding cassette sub-family A member 1 protein protein human Homo sapiens Human MACWPQLRLLLWKNLTFRRRQTCQLLLEVAWPLFIFLILISVRLSYPPYEQHECHFPNKAMPSAGTLPWVQGIICNANNPCFRYPTPGEAPGVVGNFNKSIVARLFSDARRLLLYSQKDTSMKDMRKVLRTLQQIKKSSSNLKLQDFLVDNETFSGFLYHNLSLPKSTVDKMLRADVILHKVFLQGYQLHLTSLCNGSKSEEMIQLGDQEVSELCGLPREKLAAAERVLRSNMDILKPILRTLNSTSPFPSKELAEATKTLLHSLGTLAQELFSMRSWSDMRQEVMFLTNVNSSSSSTQIYQAVSRIVCGHPEGGGLKIKSLNWYEDNNYKALFGGNGTEEDAETFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPATRQVMAEVNKTFQELAVFHDLEGMWEELSPKIWTFMENSQEMDLVRMLLDSRDNDHFWEQQLDGLDWTAQDIVAFLAKHPEDVQSSNGSVYTWREAFNETNQAIRTISRFMECVNLNKLEPIATEVWLINKSMELLDERKFWAGIVFTGITPGSIELPHHVKYKIRMDIDNVERTNKIKDGYWDPGPRADPFEDMRYVWGGFAYLQDVVEQAIIRVLTGTEKKTGVYMQQMPYPCYVDDIFLRVMSRSMPLFMTLAWIYSVAVIIKGIVYEKEARLKETMRIMGLDNSILWFSWFISSLIPLLVSAGLLVVILKLGNLLPYSDPSVVFVFLSVFAVVTILQCFLISTLFSRANLAAACGGIIYFTLYLPYVLCVAWQDYVGFTLKIFASLLSPVAFGFGCEYFALFEEQGIGVQWDNLFESPVEEDGFNLTTSVSMMLFDTFLYGVMTWYIEAVFPGQYGIPRPWYFPCTKSYWFGEESDEKSHPGSNQKRISEICMEEEPTHLKLGVSIQNLVKVYRDGMKVAVDGLALNFYEGQITSFLGHNGAGKTTTMSILTGLFPPTSGTAYILGKDIRSEMSTIRQNLGVCPQHNVLFDMLTVEEHIWFYARLKGLSEKHVKAEMEQMALDVGLPSSKLKSKTSQLSGGMQRKLSVALAFVGGSKVVILDEPTAGVDPYSRRGIWELLLKYRQGRTIILSTHHMDEADVLGDRIAIISHGKLCCVGSSLFLKNQLGTGYYLTLVKKDVESSLSSCRNSSSTVSYLKKEDSVSQSSSDAGLGSDHESDTLTIDVSAISNLIRKHVSEARLVEDIGHELTYVLPYEAAKEGAFVELFHEIDDRLSDLGISSYGISETTLEEIFLKVAEESGVDAETSDGTLPARRNRRAFGDKQSCLRPFTEDDAADPNDSDIDPESRETDLLSGMDGKGSYQVKGWKLTQQQFVALLWKRLLIARRSRKGFFAQIVLPAVFVCIALVFSLIVPPFGKYPSLELQPWMYNEQYTFVSNDAPEDTGTLELLNALTKDPGFGTRCMEGNPIPDTPCQAGEEEWTTAPVPQTIMDLFQNGNWTMQNPSPACQCSSDKIKKMLPVCPPGAGGLPPPQRKQNTADILQDLTGRNISDYLVKTYVQIIAKSLKNKIWVNEFRYGGFSLGVSNTQALPPSQEVNDAIKQMKKHLKLAKDSSADRFLNSLGRFMTGLDTKNNVKVWFNNKGWHAISSFLNVINNAILRANLQKGENPSHYGITAFNHPLNLTKQQLSEVALMTTSVDVLVSICVIFAMSFVPASFVVFLIQERVSKAKHLQFISGVKPVIYWLSNFVWDMCNYVVPATLVIIIFICFQQKSYVSSTNLPVLALLLLLYGWSITPLMYPASFVFKIPSTAYVVLTSVNLFIGINGSVATFVLELFTDNKLNNINDILKSVFLIFPHFCLGRGLIDMVKNQAMADALERFGENRFVSPLSWDLVGRNLFAMAVEGVVFFLITVLIQYRFFIRPRPVNAKLSPLNDEDEDVRRERQRILDGGGQNDILEIKELTKIYRRKRKPAVDRICVGIPPGECFGLLGVNGAGKSSTFKMLTGDTTVTRGDAFLNKNSILSNIHEVHQNMGYCPQFDAITELLTGREHVEFFALLRGVPEKEVGKVGEWAIRKLGLVKYGEKYAGNYSGGNKRKLSTAMALIGGPPVVFLDEPTTGMDPKARRFLWNCALSVVKEGRSVVLTSHSMEECEALCTRMAIMVNGRFRCLGSVQHLKNRFGDGYTIVVRIAGSNPDLKPVQDFFGLAFPGSVLKEKHRNMLQYQLPSSLSSLARIFSILSQSKKRLHIEDYSVSQTTLDQVFVNFAKDQSDDDHLKDLSLHKNQTVVDVAVLTSFLQDEKVKESYV DIP protein O95477 original sequence version: 70 DIP protein O95477 original sequence version: 169 21A2CF8F3F518D6D flot1_human Flotillin-1 FLOT1 protein protein human Homo sapiens Human MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA C69E5421E565F53A abca1-bloc1s6-1 1 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig 2A bloc1s6-abca1 1 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig 2B bloc1s6-abca1-1 2 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fig 2B abca1-bloc1s6-2 2 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig 2A abca1-bloc1s6-6 3 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 3A,B,C abca1-bloc1s6-5 4 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 3D, E, F abca1-flot1-1 1 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig. 6B abca1-flot1-5 5 8 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig.6A The treatment with Lubrol WX showed colocalization of ABCA1, Syntaxin 13 and Flotillin-1 in the rafts fraction. The treatment with Triton X-100 showed colocalization of ABCA1, Syntaxin 13 and Flotillin-1 in the soluble membrane proteins fraction. abca1-stx12-1 1 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig 5B stx12-abca1 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig 5B