bhf-ucl PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. J. Biol. Chem. (0021-9258) 2004 imex curation Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) sIg7 tag in PKD2 is n-terminal according to PMID 9192675 (from ref. 6) The PKD2 fragment sequence used for the yeast two hybrid screen provided in this paper is different from the sequence found in UniProt: Paper: Ser 851-Val 968 UniProt: His 851, Ser 852-Val 968 leonidas-tsiokas@ouhsc.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The PKD2 fragment sequence used for the yeast two hybrid screen provided in this paper is different from the sequence found in UniProt: Paper: Ser 851-Val 968 UniProt: His 851, Ser 852-Val 968 The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) leonidas-tsiokas@ouhsc.edu Accepted 2014-NOV-04 by MGT mouse embryonic day 9.5 library J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. J. Biol. Chem. (0021-9258) 2004 imex curation Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) sIg7 tag in PKD2 is n-terminal according to PMID 9192675 (from ref. 6) The PKD2 fragment sequence used for the yeast two hybrid screen provided in this paper is different from the sequence found in UniProt: Paper: Ser 851-Val 968 UniProt: His 851, Ser 852-Val 968 leonidas-tsiokas@ouhsc.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) leonidas-tsiokas@ouhsc.edu Accepted 2014-NOV-04 by MGT J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. J. Biol. Chem. (0021-9258) 2004 imex curation Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) sIg7 tag in PKD2 is n-terminal according to PMID 9192675 (from ref. 6) The PKD2 fragment sequence used for the yeast two hybrid screen provided in this paper is different from the sequence found in UniProt: Paper: Ser 851-Val 968 UniProt: His 851, Ser 852-Val 968 leonidas-tsiokas@ouhsc.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down anti tag western anti tag western blot anti tag western Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) leonidas-tsiokas@ouhsc.edu Accepted 2014-NOV-04 by MGT J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. J. Biol. Chem. (0021-9258) 2004 imex curation Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) sIg7 tag in PKD2 is n-terminal according to PMID 9192675 (from ref. 6) The PKD2 fragment sequence used for the yeast two hybrid screen provided in this paper is different from the sequence found in UniProt: Paper: Ser 851-Val 968 UniProt: His 851, Ser 852-Val 968 leonidas-tsiokas@ouhsc.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The PKD2 fragment sequence used for the yeast two hybrid screen provided in this paper is different from the sequence found in UniProt: Paper: Ser 851-Val 968 UniProt: His 851, Ser 852-Val 968 The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) leonidas-tsiokas@ouhsc.edu Accepted 2014-NOV-04 by MGT J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. PKD2 interacts and co-localizes with mDia1 to mitotic spindles of dividing cells: role of mDia1 IN PKD2 localization to mitotic spindles. J. Biol. Chem. (0021-9258) 2004 imex curation Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) sIg7 tag in PKD2 is n-terminal according to PMID 9192675 (from ref. 6) The PKD2 fragment sequence used for the yeast two hybrid screen provided in this paper is different from the sequence found in UniProt: Paper: Ser 851-Val 968 UniProt: His 851, Ser 852-Val 968 leonidas-tsiokas@ouhsc.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions pig-llcpk Sus scrofa kidney cell line LLCPK llcpk Porcine kidney cell line confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Rundle D.R., Gorbsky G., Tsiokas L. Cardiac - Interactions involving cardiac related proteins The authors have stated that mDia1 is of Murine origin, and that the aa. range of delN3mDia1 used in Fig. 2C was (543-1255aa.) leonidas-tsiokas@ouhsc.edu Accepted 2014-NOV-04 by MGT J. Biol. Chem. (0021-9258) 2004 Only protein-protein interactions imex curation diap1_human Protein diaphanous homolog 1 DIAPH1 DIAP1 Diaphanous-related formin-1 protein protein human Homo sapiens Human MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS 9C8273DE4748564F mdia1_pig_protein mammalian diaphanous 1 pig protein protein protein pig Sus scrofa (Pig) Pig protein similar to the entry for O08808 q13563-1 Polycystin-2 PKD2 Autosomal dominant polycystic kidney disease type II protein Polycystic kidney disease 2 protein Polycystwin R48321 TRPP2 Transient receptor potential cation channel subfamily P member 2 protein protein human Homo sapiens Human MVNSSRVQPQQPGDAKRPPAPRAPDPGRLMAGCAAVGASLAAPGGLCEQRGLEIEMQRIRQAAARDPPAGAAASPSPPLSSCSRQAWSRDNPGFEAEEEEEEVEGEEGGMVVEMDVEWRPGSRRSAASSAVSSVGARSRGLGGYHGAGHPSGRRRRREDQGPPCPSPVGGGDPLHRHLPLEGQPPRVAWAERLVRGLRGLWGTRLMEESSTNREKYLKSVLRELVTYLLFLIVLCILTYGMMSSNVYYYTRMMSQLFLDTPVSKTEKTNFKTLSSMEDFWKFTEGSLLDGLYWKMQPSNQTEADNRSFIFYENLLLGVPRIRQLRVRNGSCSIPQDLRDEIKECYDVYSVSSEDRAPFGPRNGTAWIYTSEKDLNGSSHWGIIATYSGAGYYLDLSRTREETAAQVASLKKNVWLDRGTRATFIDFSVYNANINLFCVVRLLVEFPATGGVIPSWQFQPLKLIRYVTTFDFFLAACEIIFCFFIFYYVVEEILEIRIHKLHYFRSFWNCLDVVIVVLSVVAIGINIYRTSNVEVLLQFLEDQNTFPNFEHLAYWQIQFNNIAAVTVFFVWIKLFKFINFNRTMSQLSTTMSRCAKDLFGFAIMFFIIFLAYAQLAYLVFGTQVDDFSTFQECIFTQFRIILGDINFAEIEEANRVLGPIYFTTFVFFMFFILLNMFLAIINDTYSEVKSDLAQQKAEMELSDLIRKGYHKALVKLKLKKNTVDDISESLRQGGGKLNFDELRQDLKGKGHTDAEIEAIFTKYDQDGDQELTEHEHQQMRDDLEKEREDLDLDHSSLPRPMSSRSFPRSLDDSEEDDDEDSGHSSRRRGSISSGVSYEEFQVLVRRVDRMEHSIGSIVSKIDAVIVKLEIMERAKLKRREVLGRLLDGVAEDERLGRDSEIHREQMERLVREELERWESDDAASQISHGLGTPVGLNGQPRPRSSRPSSSQSTEGMEGAGGNGSSNVHV DIP protein Q13563-1 original sequence version: 146 DIP protein Q13563 original sequence version: 144 DIP protein Q13563-1 original sequence version: 128 DIP protein Q13563-1 original sequence version: 133 F8D2E760EBEA8B47 f2vya1_pig PKD2 protein protein pig Sus scrofa (Pig) Pig MVNSSRVQPQQPGDAQRPAAPRTAGPGRLMAGGGAAGAGLAAPGGLREQRGLEIEMERIRQAAARDPPAGASASPSPPLSSCSRQAWSRDNPGFEAEEEEEEDEVEGEEGGMVVEMDVEWRPGSRRSAASSAVSSAGARGRGLGSFHGAGHPSGRRRRREDQGPPSPSPAGGGDPLHRHLPLDGQPPRVAWAERLVRGLRGLWGTRLMEESSTDRERYLKSVLRELATYLLFLIVLCVLTYGMMSSSVYYYTRIMSQLFLDTPVSRTEKTNFKTLSSVEDFWKFAEGALLDGLYWKTQPSNGTEAHNRSFIYYENLLLGVPRIRQLRVRNGSCSIPQDLRDEIKECYDVYSVSSEDRAPFGPRNGTAWIYTSEKDLNGSSHWGMLTTYSGAGYYLDLSRTREETAAQLAHLRKHAWLDRGTRATFIDFSVYNANINLFCVIRLLVEFPATGGVIPSWQFQPVKLIQYVTTFDFFLAACEIIFCFFILYYVVEEILEIRIHKLHYFRSFWNCLDVVIIVLSVAAIGINIHRTSNVEMLLQFLEDQNTYPNFENLAYWQIQFNNIAAVIVFFVWIKLFKFISFNRTMSQLSTTMSRCAKDLFGFAIMFFIIFLAYAQLAYLVFGTQVDDFSTFQECIFTQFRIILGDINFAEIEEANRVLGPIYFTTFVFFTFFILLNMFLAIINDTYSEVKSDLAQQKAEMELSDLIRKGYHKALVKLKLKKNTVDDISESLRQGGGKLNFDELRQDLKGKGHTDAEIEAIFTKYDQDGDQELTEREHQQMRDDLEKEREDLDLEHSSLPRPMSSRSFPRSLDDSEEDDDDDSGHSSRRRGSISSGVSYEEFQVLVRRVDRMEHSIGSIVSKIDAVIVKLEIMERNKLKRREVLGRLLDGVAEDERLGRDSEIHREQMERLVREELERWESDDAASQISHGLGTPVGLNGQPRPRSSRPSSSQSTEGMEGGGGNGSADIHV 8F122F2AD03BB6AC diap1_mouse Protein diaphanous homolog 1 Diaphanous-related formin-1 p140mDIA Diaph1 Diap1 protein protein mouse Mus musculus Mouse MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS DIP protein O08808 original sequence version: 62 DIP protein O08808 original sequence version: 141 09404164873CA7C1 pkd2_human Polycystin-2 PKD2 Polycystic kidney disease 2 protein Autosomal dominant polycystic kidney disease type II protein Polycystwin R48321 TRPP2 Transient receptor potential cation channel subfamily P member 2 protein protein human Homo sapiens Human MVNSSRVQPQQPGDAKRPPAPRAPDPGRLMAGCAAVGASLAAPGGLCEQRGLEIEMQRIRQAAARDPPAGAAASPSPPLSSCSRQAWSRDNPGFEAEEEEEEVEGEEGGMVVEMDVEWRPGSRRSAASSAVSSVGARSRGLGGYHGAGHPSGRRRRREDQGPPCPSPVGGGDPLHRHLPLEGQPPRVAWAERLVRGLRGLWGTRLMEESSTNREKYLKSVLRELVTYLLFLIVLCILTYGMMSSNVYYYTRMMSQLFLDTPVSKTEKTNFKTLSSMEDFWKFTEGSLLDGLYWKMQPSNQTEADNRSFIFYENLLLGVPRIRQLRVRNGSCSIPQDLRDEIKECYDVYSVSSEDRAPFGPRNGTAWIYTSEKDLNGSSHWGIIATYSGAGYYLDLSRTREETAAQVASLKKNVWLDRGTRATFIDFSVYNANINLFCVVRLLVEFPATGGVIPSWQFQPLKLIRYVTTFDFFLAACEIIFCFFIFYYVVEEILEIRIHKLHYFRSFWNCLDVVIVVLSVVAIGINIYRTSNVEVLLQFLEDQNTFPNFEHLAYWQIQFNNIAAVTVFFVWIKLFKFINFNRTMSQLSTTMSRCAKDLFGFAIMFFIIFLAYAQLAYLVFGTQVDDFSTFQECIFTQFRIILGDINFAEIEEANRVLGPIYFTTFVFFMFFILLNMFLAIINDTYSEVKSDLAQQKAEMELSDLIRKGYHKALVKLKLKKNTVDDISESLRQGGGKLNFDELRQDLKGKGHTDAEIEAIFTKYDQDGDQELTEHEHQQMRDDLEKEREDLDLDHSSLPRPMSSRSFPRSLDDSEEDDDEDSGHSSRRRGSISSGVSYEEFQVLVRRVDRMEHSIGSIVSKIDAVIVKLEIMERAKLKRREVLGRLLDGVAEDERLGRDSEIHREQMERLVREELERWESDDAASQISHGLGTPVGLNGQPRPRSSRPSSSQSTEGMEGAGGNGSSNVHV Functions as a calcium permeable cation channel. PKD1 and PKD2 may function through a common signaling pathway that is necessary for normal tubulogenesis Defects in PKD2 are the cause of polycystic kidney disease autosomal dominant type 2 (ADPKD2) [MIM:173900]. ADPKD2 represents approximately 15% of the cases of ADPKD, a common genetic disease affecting about 1:400 to 1:1000 individuals. ADPKD is characterized by progressive formation and enlargement of cysts in both kidneys, typically leading to end-stage renal disease in adult life. Cysts also occurs in the liver and other organs. ADPKD2 is clinically milder than ADPKD1 but it has a deleterious impact on overall life expectancy mint F8D2E760EBEA8B47 pkd2-diaph1 1 10 unspecified role unspecified role prey prey rhoa binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 14 16 physical association physical association pkd2-diaph1-1 4 10 unspecified role unspecified role prey prey rhoa binding domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 19 21 physical association physical association diaph1-pkd2-2 2 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Fg. 2A mdia1-pkd2-1 5 9 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3 pkd2-diaph1-2 3 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain igg tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 10 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 30 34 physical association physical association Fig.2C