IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus. Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 imex curation Liu Z., Zhang J., Sharma M.R., Li P., Chen S.R., Wagenknecht T. Cardiac - Interactions involving cardiac related proteins liuz@wadsworth.org 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Liu Z., Zhang J., Sharma M.R., Li P., Chen S.R., Wagenknecht T. Cardiac - Interactions involving cardiac related proteins liuz@wadsworth.org Accepted 2014-AUG-26 by MGT Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 Only protein-protein interactions imex curation Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus. Three-dimensional reconstruction of the recombinant type 3 ryanodine receptor and localization of its amino terminus. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 imex curation Liu Z., Zhang J., Sharma M.R., Li P., Chen S.R., Wagenknecht T. Cardiac - Interactions involving cardiac related proteins liuz@wadsworth.org 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro saturation binding saturation binding tag visualisation tag visualisation Liu Z., Zhang J., Sharma M.R., Li P., Chen S.R., Wagenknecht T. Cardiac - Interactions involving cardiac related proteins liuz@wadsworth.org Accepted 2014-AUG-26 by MGT Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2001 Only protein-protein interactions imex curation ryr3_rabit Ryanodine receptor 3 Brain ryanodine receptor-calcium release channel Brain-type ryanodine receptor Type 3 ryanodine receptor protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MAEGGEGGEDEIQFLRTEDEVVLQCIATVHKEQRKFCLAAEGLGNRLCFLEPTSEAKFIPPDLCVCNFVLEQSLSVRALQEMLANTGENGGEGAAQGGGHRTLLYGHAILLRHSFSGMYLTCLTTSRSQTDKLAFDVGLREHATGEACWWTIHPASKQRSEGEKVRIGDDLILVSVSSERYLHLSISNGNIQVDASFMQTLWNVHPTCSGSSIEEGYLLGGHVVRLFHGHDECLTIPSTDQNDSQHRRIFYEAGGAGTRARSLWRVEPLRISWSGSNIRWGQAFRLRHLTTGHYLALTEDQGLLLQDRGKADTKSTAFSFRPSKETKEKLDSSHKRDIEGMGVPEIKYGDSVCFVQHIASGLWVTYKAQDAKTSRLGPLKRKVILHQEGHMDDGLTLQRCQREESQAARIIRNTTALFSQFVSGNNRTAAPVTLPIEEVLQTLHDLIAYFQPPEEEMQHEDKQNKLRSLKNRQNLFKEEGMLALVLNCIDRLNIYNSVAHFAGIAREESGMAWKEVLSLLYKLLAALIRGNRNTCAQFSNNLDWLISKLDRLESSSGILEVLHCILIESPEALNLIAEGHIKSIISLLDKHGRNHKVLDVLCSLCLCNGVAVRANQNLICDNLLPRRNLLLQTRLINDVTSIRPNIFLGVAEGSAQYKKWYFELIIDQVDPFLTAEPTHLRVGWASSSGYAPYPGGGEGWGGNGVGDDLYSYGFDGLHLWSGRIPRAVASINQHLLKSDDVVSCCLDLGVPSISFRINGQPVQGMFENFNTDGLFFPVMSFSAGVKVRFLMGGRHGEFKFLPPSGYAPCYEALLPKEKMRLEPVKEYKRDAEGVRDLLGTTQFLSQASFIPCPIDTSQVVLPPHLEKIRDRLAENIHELWGMNKIELGWTFGKMRDDNKRQHPCLVEFSKLPETEKNYNLQMSTETLKTLLALGCHIAHVNPAAEEDLKKVKLPKNYMMSNGYKPAPLDLSDVKLLPPQEILVDKLAENAHNVWAKDRIKQGWTYGIQQDLKNKRNPRLVPYALLDERTKKSNRDSLREAVRTFVGYGYNIEPSDQELADPAVEKVSIDKIRFFRVERSYAVRSGKWYFEFEVVTGGDMRVGWARPGCRPDIELGPMTKPLCLKAAGASVGTKVVGILGVPWQPGDVVGCMINLDDASMIFTLNGELLITNKGSELAFADYEIENGFVPICSLGLSQIGRMNLGTDASTFKFYTMCGLQEGFEPFAVNMNRDVAMWFSKRLPTFVNVPKDHPHIEVVRIDGTMDSPPCLKVTHKTFGTQNSNANMIYCRLSMPVECHSSFSHSPCLDSEAFQKRKQMQEILSHTTTQCFYSIRIFAGQDPSCVWVGWVTPDYHLYSEKFDLNKNCTVTVTLGDERGRVHESVKRSNCYMVWGGDVVASSQRSSRSNVDLEIGCLVDLAMGMLSFSANGKELGTCYQVEPNTKVFPAVFLQPTSTSLFQFELGKLKNAMPLSAAIFKSEEKNPVPQCPPRLDVQTIQPVLWSRMPNSFLKVETERVSERHGWVVQCLEPLQMMALHIPEENRCVDILELCEQEDLMQFHYHTLRLYSAVCALGNSRVAYALCSHVDLSQLFHAIDNKYLPGLLRSGFYDLLISIHLANAKERKLMMKNEYIIPITSTTRNIRLYPDESKKHGLPGVGPRTCLKPGFKFSTPCFVVTNEERQKQSPEIPLEILKMKALSMLTEAVQCSGAHIRDPVGGSVEFQFVPVLKLVGTLLVMGVFCDDDVRQILLLIDPSVFGEHSADTEEGAEKEEVSQVEEKAVEAGEKTSKEARKEAPVRGLLQTRLPESVKLQMCELLSYLCDCELQHRVEAIVAFGDIYVSKLQANQKFRYNELMQALNMSAALTARKTREFRSPPQEQINMLLNFQLGENCPCPEEIREELYDFHEDLLVHCGVPLEEEEEEEEDTSWTGKLRTLVYKIKGPPKPEKEQPTEEEERCPTTLKELISQTMIRWAQEDQIQDAELVRMMFNLLRRQYDSIGELLQALRKTYTISHASVSDTINLLAALGQIRSLLSVRMGREEELLMINGLGDIMNNKVFYQHPNLMRVLGMHETVMEVMVNVLGTEKSQIAFPKMVASCCRFLCYFCRISRQNQKAMFEHLSYLLENSSVGLASPSMRGSTPLDVAASSVMDNNELALGLEEPDLEKVVTYLAGCGLQSCPMLLAKGYPDVGWNPIEGERYLSFLRFAVFVNSESVEENASVVVKLLIRRPECFGPALRGEGGNGLLAAMQGAIKISESPALDLPSQGYKREVPEDGEEEEEIVHMGNAIMSFYSALIDLLGRCAPEMHLIQTGKGEAIRIRSILRSLVPTEDLVGIISIPLKLPSLNKDGSVSEPDMAANFCPDHKAPMVLFLDRVYGIKDQTFLLHLLEVGFLPDLRASASLDTVALSTTESALALNRYICSAVLPLLTRCAPLFAGTEHYTSLIDSTLQTIYRLSKGRSLTKAQRDTIEECLLAICNHLRPSMLQQLLRRLVFDVPQLNDYCKMPLKLLTNHFEQCWKYYCLPSGWGSYGLAVEEELHLTEKLFWGIFDSLSHKKYDPDLFRMSLPCLSAIAGALPPDYLDTRITATLEKQVSVDADGNFDPKPINTINFSLPEKLEYIVTKYAEHSHDKWACEKSQSGWKYGISLDENVKTHPLIRPFKTLTEKEKEIYRWPARESLKTMLAVGWTVERTKEGEALVQLRENEKLRSVSQTSQGNSYNPAPLDLSNVVLSRELQGMVEVVAENYHNIWAKKKKLELESKGGGSHPLLVPYDTLTAKEKFRDREKAQDLFKFLQVNGVIVSRGMKDMELDAFSMEKRFAYKFLKKILKYVDSAQEFIAHLEAIVSSGKTEKSPHDQEIKFFAKVLLPLVDQYFTNHRLYFLSSPLKPLSSSGYASHKEKEMVASLFCKLAALVRHRISLFGSDSTTMVSCLHILAQTLDTRTVMKSGSELVKAGLRAFFESAAEDLEKTSENLKLGKFTHSRTQIKGVSQNINYTTVALLPILTSIFEHVAQHQFGVDLLLGDVQISCYRILCSLYSLGTGKNIYVERQRPALGECLASLAAAIPVAFLEPTLNRYNALSVFNTKTPRERSILGMPDTVEEMCPDIPQLEGLMKEINDLAESGARYTEMPHVIEVILPMLCNYLSYWWERGPENLSPSTGPCCSKVTSEHLSLILGNILKIINNNLGIDEASWMKRIAVYAQPIISKARPDLLRSHFIPTLEKLKKKAVKTVQEEEQLKADGKGDTQEAELLILDEFAILCRDLYAFYPMLIRYVDNNRSNWLKSPDGDSDQLFRMVAEVFILWCKSHNFKREEQNFVIQNEINNLAFLTGDSKSKMSKAMQVKSGGQDQERKKTKRRGDLYSIQTSLIVAALKKMLPIGLNMCTPGDQELISLAKSRYSYRDTDEEVKEHLRNNLHLQEKSDDPAVKWQLNLYKDVLKSEEPSNPEKTVERVQRISAAVFHLEQVEQPLRSKKAVWHKLLSKQRKRAVVACFRMAPLYNLPRHRSINLFLHGYQRFWIETEEYSFEEKLVQDLAKSPKVEEEEEEEMEKQPDPLHQIILHFSRNALTERSKLEDDPLYTSYSSMMAKSCQSGEDEEEEDKEKTFEEKEMEKQKTLYQQARLHERGAAEMVLQMISASKGEMSPMVVETLKLGIAILNGGNAGVQQKMLDYLKVKKDAGFFQSLSGLMQSCSVLDLNAFERQNKAEGLGMVTEEGTLIVRERGEKVLQNDEFTRDLFRFLQLLCEGHNSDFQNFLRTQMGNTTTVNVIISTVDYLLRLQESISDFYWYYSGKDIIDESGQHNFSKALAVTKQIFNSLTEYIQGPCIGNQQSLAHSRLWDAVVGFLHVFANMQMKLSQDSSQIELLKELLDLLQDMVVMLLSLLEGNVVNGTIGKQMVDTLVESSTNVEMILKFFDMFLKLKDLTSSDTFKEYDPDGKGIISKKEFQKAMEGQKQYTQSEIDFLLSCAEADENDMFNYIDFVDRFHEPAKDIGFNVAVLLTNLSEHMPNDSRLKCLLDPAESVLNYFEPYLGRIEIMGGAKKIERVYFEISESSRTQWEKPQVKESKRQFIFDVVNEGGEQEKMELFVNFCEDTIFEMQLASQISESDSADRPEEEEGDEESSYVLEINGEEEEDKSFESASAFAMACASLKRNITNLLRKATLKNLRKQYRNVKKMTAKELVKVFFSFFWMLFVGLFQLFFTIVGGIFQILWSTVFGGGLVEGAKNIRVTKILGDMPDPTQFGIHDDAMEAERAEVAEAGITTELVHFVKGERGDTELMSDLFGLHPKKEGGVKHGPEVGLGDLSEIIGKDEPPTLESTVRKKRKAQAAETKAEHEAEGKVESEKADLEDGEKEDKAKEEERAEYLWAEVTKKKKRRRGQKVEKPEAFMANFFKGLEIYQTKLLHYLARNFYNLRFLALFVAFAINFILLFYKVTEEPLEEETEDVANLWNSLNDEEEEEAMVFFVLQESTGYMAPTLRALAVVHTIISLVCVVGYYCLKVPLVVFKREKEIARKLEFDGLYITEQPSEDDIKGQWDRLVINTPSFPHNYWDKFVKRKVINKYGDLYGAERIAELLGLDKNALDFSPVEETTAEAASLVSWLSSIDMKYHIWKLGVVFTDNSFLYLAWYTTMSVLGHYNNFFFAAHLLDIAMGFKTLRTILSSVTHNGKQLVLTVGLLAVVVYLYTVVAFNFFRKFYNKSEDDDEPDMKCDDMMTCYLFHMYVGVRAGGGIGDEIEDPAGDPYEMYRIVFDITFFFFVIVILLAIIQGLIIDAFGELRDQQEQVREDMETKCFICGIGNDYFDTTPHGFETHTLQEHNLANYLFFLMYLINKDETEHTGQESYVWKMYQERCWDFFPAGDCFRKQYEDQLG D1DC6B85CD6B93E6 ryanodine Ryanodine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ryr3-ryr3 1 3 unspecified role unspecified role neutral component neutral component human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Fig. 3 ryr3-1 1 3 unspecified role unspecified role neutral component neutral component n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range glu4318_glu-gln-lys-leu-ile-ser-glu-glu-asp-leu_ser4319 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Fig. 3 ryr3-ryanodine 2 4 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ryasp Ryania speciosa 3 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range glu4318_glu-gln-lys-leu-ile-ser-glu-glu-asp-leu_ser4319 myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Fig. 2 0.8 mM Ca 2+ 2.5 mM caffeine 5 mM Mg2+ Bmax=447 pmol/mg ryr3-ryanodine-1 2 4 unspecified role unspecified role prey prey region 3h radiolabel 3h radiolabel 3H tritium H3 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ryasp Ryania speciosa 3 unspecified role unspecified role bait bait human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction Fig. 2 0.8 mM Ca 2+ 2.5 mM caffeine 5 mM Mg 2+ Bmax=413 pmol/mg 1.9 nM