bhf-ucl Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu 30-Sept-2013: Contacted by IntAct-Help. unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Only protein-protein interactions rat-rat-1 Rattus norvegicus embryonic fibroblast cells rat-1 Rat-1 embryonic fibroblasts anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu Accepted 2013-SEP-26 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu 30-Sept-2013: Contacted by IntAct-Help. unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu Accepted 2013-SEP-26 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu 30-Sept-2013: Contacted by IntAct-Help. unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu Accepted 2013-SEP-26 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Phosphorylation and inactivation of glycogen synthase kinase 3 by protein kinase A. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu 30-Sept-2013: Contacted by IntAct-Help. unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Fang X., Yu S.X., Lu Y., Bast R.C., Woodgett J.R., Mills G.B. Cardiac - Interactions involving cardiac related proteins gmills@notes.mdacc.tmc.edu Accepted 2013-SEP-26 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. unable to determine species of cAMP-dependent protein kinase A in PKA assay kit (Upstate Biotechnology), therefore Fig 4B not annotated Anti-GSK-3α (sheep anti-rat GSK-3α; Upstate Biotechnology) Anti-GSK-3β (mouse monoclonal anti-rat GSK-3β; Transduction Laboratories, Lexington, KY) Rabbit polyclonal antibodies against PKAc or the PKA regulatory subunit RII (PKA RII) (Santa Cruz Biotechnology) were used for PKAc or PKA RII immunoprecipitation and Western blotting. Antibodies to PKA RII do not identify specific isoforms therefore PPIs using this antibody not annotated. PKB was immunoprecipitated with a sheep polyclonal anti-rat PKB (Upstate Biotechnology). Dr. B. Su (M. D. Anderson Cancer Center) provided anti-HA monoclonal antibody Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2000 imex curation gsk3a_rat Glycogen synthase kinase-3 alpha Gsk3a Factor A Serine/threonine-protein kinase GSK3A protein protein rat Rattus norvegicus (Rat) Rat MSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGGPGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPSGSGGGGSGGPGAGTSFPPPGVKLGRDSGKVTTVVATLGQGPERSQEVAYTDIKVIGNGSFGVVYQARLAETRELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDELYLNLVLEYVPETVYRVARHFTKAKLIIPIIYVKVYMYQLFRSLAYIHSQGVCHRDIKPQNLLVDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFKSRTPPEAIALCSSLLEYTPSSRLSPLEACAHSFFDELRSLGTQLPNNRPLPPLFNFSPGELSIQPSLNAILIPPHLRSPSGPATLTSSSQALTETQTGQDWQAPDATPTLTNSS A98DC1C5C7F7B716 kapca_mouse cAMP-dependent protein kinase catalytic subunit alpha Prkaca Pkaca protein protein mouse Mus musculus Mouse MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF DIP protein P05132 original sequence version: 185 DIP protein P05132 original sequence version: 186 DIP protein P05132-1 original sequence version: 145 DIP protein P05132-1 original sequence version: 193 DIP protein P05132-1 original sequence version: 199 DIP protein P05132 original sequence version: 163 DIP protein P05132 original sequence version: 192 DIP protein P05132-1 original sequence version: 197 DIP protein P05132-1 original sequence version: 149 02F85D66EB21A1FA gsk3b_mouse Glycogen synthase kinase-3 beta Gsk3b Serine/threonine-protein kinase GSK3B protein protein mouse Mus musculus Mouse MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASPPANATAASDTNAGDRGQTNNAASASASNST DIP protein Q9WV60 original sequence version: 159 200C3FD1B38B4883 gsk3b_human Glycogen synthase kinase-3 beta GSK3B Serine/threonine-protein kinase GSK3B protein protein human Homo sapiens Human MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST DIP protein P49841 original sequence version: 109 DIP protein P49841 original sequence version: 136 DIP protein P49841 original sequence version: 143 DIP protein P49841 original sequence version: 141 DIP protein P49841 original sequence version: 196 DIP protein P49841 original sequence version: 159 DIP protein P49841 original sequence version: 151 4ACC24D00CDBB9C3 kapca_human cAMP-dependent protein kinase catalytic subunit alpha PRKACA PKACA protein protein human Homo sapiens Human MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF DIP protein P17612 original sequence version: 171 DIP protein P17612 original sequence version: 175 DIP protein P17612 original sequence version: 178 DIP protein P17612-1 original sequence version: 177 DIP protein P17612 original sequence version: 186 DIP protein P17612 original sequence version: 102 DIP protein P17612-1 original sequence version: 102 BF6D3ECD2614E5AB kapca_rat cAMP-dependent protein kinase catalytic subunit alpha Prkaca protein protein rat Rattus norvegicus (Rat) Rat MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWEDPSQNTAQLDHFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF DIP protein P27791 original sequence version: 143 4763ACD074AB2384 gsk3a-prkaca 4 9 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate 5 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate physical association physical association Fig 3A gsk3a-prkaca-2 1 10 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate 5 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate physical association physical association Fig 3A gsk3b-prkaca 4 9 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate 8 monoclonal western monoclonal antibody western blot monoclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3A gsk3b-prkaca-2 3 6 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate 8 monoclonal western monoclonal antibody western blot monoclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3A gsk3b-prkaca-3 2 9 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate 8 monoclonal western monoclonal antibody western blot monoclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3B prkaca-gsk3b 4 9 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate 8 monoclonal western monoclonal antibody western blot monoclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3B gsk3b-prkaca-4 4 9 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate 8 monoclonal western monoclonal antibody western blot monoclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate physical association physical association Fig 3C forskolin gsk3b-prkaca-6 3 7 monoclonal western monoclonal antibody western blot monoclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait cell lysate cell lysate 6 polyclonal western polyclonal antibody western blot polyclonal western western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate physical association physical association Fig 3C forskolin