IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure of the dimerized hormone-binding domain of a guanylyl-cyclase-coupled receptor. Structure of the dimerized hormone-binding domain of a guanylyl-cyclase-coupled receptor. Nature (0028-0836) 2000 imex curation van den Akker F., Zhang X., Miyagi M., Huo X., Misono K.S., Yee V.C. Cardiac - Interactions involving cardiac related proteins yeev@ccf.org, misonok@ccf.org 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined van den Akker F., Zhang X., Miyagi M., Huo X., Misono K.S., Yee V.C. Cardiac - Interactions involving cardiac related proteins yeev@ccf.org, misonok@ccf.org Accepted 2015-JAN-21 by MGT Nature (0028-0836) 2000 Only protein-protein interactions imex curation n-acetyl-l-glutamate(2-) N-acetyl-L-glutamate(2-) (S)-2-(acetylamino)pentanedioate acetyl-L-glutamate N-Acetyl-L-glutamate NAG (2S)-2-acetamidopentanedioate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) RFMMMVDNIPUKGG-YFKPBYRVSA-L RFMMMVDNIPUKGG-YFKPBYRVSA-L anpra_rat Atrial natriuretic peptide receptor 1 Atrial natriuretic peptide receptor type A Guanylate cyclase A Npr1 protein protein rat Rattus norvegicus (Rat) Rat MPGSRRVRPRLRALLLLPPLLLLRGGHASDLTVAVVLPLTNTSYPWSWARVGPAVELALARVKARPDLLPGWTVRMVLGSSENAAGVCSDTAAPLAAVDLKWEHSPAVFLGPGCVYSAAPVGRFTAHWRVPLLTAGAPALGIGVKDEYALTTRTGPSHVKLGDFVTALHRRLGWEHQALVLYADRLGDDRPCFFIVEGLYMRVRERLNITVNHQEFVEGDPDHYPKLLRAVRRKGRVIYICSSPDAFRNLMLLALNAGLTGEDYVFFHLDVFGQSLKSAQGLVPQKPWERGDGQDRSARQAFQAAKIITYKEPDNPEYLEFLKQLKLLADKKFNFTVEDGLKNIIPASFHDGLLLYVQAVTETLAQGGTVTDGENITQRMWNRSFQGVTGYLKIDRNGDRDTDFSLWDMDPETGAFRVVLNYNGTSQELMAVSEHKLYWPLGYPPPDVPKCGFDNEDPACNQDHFSTLEVLALVGSLSLISFLIVSFFIYRKMQLEKELVSELWRVRWEDLQPSSLERHLRSAGSRLTLSGRGSNYGSLLTTEGQFQVFAKTAYYKGNLVAVKRVNRKRIELTRKVLFELKHMRDVQNEHLTRFVGACTDPPNICILTEYCPRGSLQDILENESITLDWMFRYSLTNDIVKGMLFLHNGAICSHGNLKSSNCVVDGRFVLKITDYGLESFRDPEPEQGHTLFAKKLWTAPELLRMASPPARGSQAGDVYSFGIILQEIALRSGVFYVEGLDLSPKEIIERVTRGEQPPFRPSMDLQSHLEELGQLMQRCWAEDPQERPPFQQIRLALRKFNKENSSNILDNLLSRMEQYANNLEELVEERTQAYLEEKRKAEALLYQILPHSVAEQLKRGETVQAEAFDSVTIYFSDIVGFTALSAESTPMQVVTLLNDLYTCFDAVIDNFDVYKVETIGDAYMVVSGLPVRNGQLHAREVARMALALLDAVRSFRIRHRPQEQLRLRIGIHTGPVCAGVVGLKMPRYCLFGDTVNTASRMESNGEALKIHLSSETKAVLEEFDGFELELRGDVEMKGKGKVRTYWLLGERGCSTRG mint Receptor for atrial natriuretic peptide (NPPA/ANF). Has guanylate cyclase activity on binding of NPPA/ANF 9EA9AE685AC05816 n_acetyl_l_glutamate_2__-npr1 1 3 unspecified role unspecified role neutral component neutral component ecd sufficient to bind sufficient binding region sufficient to bind range range range range crigr-cho-c1 GRP78 overexpressing, DHFR-deficient CHO cells cho-c1 GRP78-expressing, DHFR-deficient CHO cells (C-.1) 2 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1, Fig.1 Rcryst: 19.8 Rfree: 22.5 2.0A NAG (CHEBI:44337)