IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mhcc97h human-MHCC97H -highly metastatic subclone of MHCC97 Human mhcc97h MHCC97H -highly metastatic subclone of MHCC97 confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-plc_prf_5 Homo sapiens PLC/PRF/5 malignant liver hepatoma cell line plc-prf-5 PLC/PRF/5 Human malignant liver hepatoma cell line This cell line is often referred to as "PLC5" confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer It has been assumed that the cell line "PLC" refers to PLC/PRF5 cell line, as no other similarly named cell line can be found. The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-plc_prf_5 Homo sapiens PLC/PRF/5 malignant liver hepatoma cell line plc-prf-5 PLC/PRF/5 Human malignant liver hepatoma cell line This cell line is often referred to as "PLC5" anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The authors have stated that the "PLC" cell line was used. The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hep_3b Homo sapiens hepatocellular carcinoma (HCC) cells (p53-null). hep_3b human hepatocellular carcinoma (HCC) cells (p53-null). anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-plc_prf_5 Homo sapiens PLC/PRF/5 malignant liver hepatoma cell line plc-prf-5 PLC/PRF/5 Human malignant liver hepatoma cell line This cell line is often referred to as "PLC5" anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer It has been assumed that the cell line "PLC" refers to PLC/PRF5 cell line, as no other similarly named cell line can be found. The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-plc_prf_5 Homo sapiens PLC/PRF/5 malignant liver hepatoma cell line plc-prf-5 PLC/PRF/5 Human malignant liver hepatoma cell line This cell line is often referred to as "PLC5" anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hep_3b Homo sapiens hepatocellular carcinoma (HCC) cells (p53-null). hep_3b human hepatocellular carcinoma (HCC) cells (p53-null). anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mhcc97h human-MHCC97H -highly metastatic subclone of MHCC97 Human mhcc97h MHCC97H -highly metastatic subclone of MHCC97 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. GOLM1 Modulates EGFR/RTK Cell-Surface Recycling to Drive Hepatocellular Carcinoma Metastasis. Cancer cell 2016 imex curation Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-plc_prf_5 Homo sapiens PLC/PRF/5 malignant liver hepatoma cell line plc-prf-5 PLC/PRF/5 Human malignant liver hepatoma cell line This cell line is often referred to as "PLC5" confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Ye QH., Zhu WW., Zhang JB., Qin Y., Lu M., Lin GL., Guo L., Zhang B., Lin ZH., Roessler S., Forgues M., Jia HL., Lu L., Zhang XF., Lian BF., Xie L., Dong QZ., Tang ZY., Wang XW., Qin LX. Cancer - Interactions investigated in the context of cancer It has been assumed that the cell line "PLC" refers to PLC/PRF5 cell line, as no other similarly named cell line can be found. The Rab 11 antibody would detect both the Rab11A and Rab11B forms - they are almost identical in sequence for the immunogen range used. Also this is described as an "In vitro" experiment - I would have thought this was an anti-tag IP in PLC cells? GOLM1-Flag is of Human origin, as shown by sequence length in figures 5D and 7A. Results from figure 6I have not been added due to the lack of specificity of the Rab11 antibody used. xw3u@nih.gov,qinlx@fudan.edu.cn Accepted 2017-OCT-18 AT 13:04 BST AT 13:04 BST by PPORRAS Cancer cell 2016 Only protein-protein interactions imex curation imex curation cav3_human Caveolin-3 CAV3 M-caveolin protein protein human Homo sapiens Human MMAEEHTDLEAQIVKDIHCKEIDLVNRDPKNINEDIVKVDFEDVIAEPVGTYSFDGVWKVSYTTFTVSKYWCYRLLSTLLGVPLALLWGFLFACISFCHIWAVVPCIKSYLIEIQCISHIYSLCIRTFCNPLFAALGQVCSSIKVVLRKEV C695E14F5B8F4753 cbl_human E3 ubiquitin-protein ligase CBL Signal transduction protein CBL Proto-oncogene c-Cbl Casitas B-lineage lymphoma proto-oncogene RING finger protein 55 CBL CBL2 RNF55 RING-type E3 ubiquitin transferase CBL protein protein human Homo sapiens Human MAGNVKKSSGAGGGSGSGGSGSGGLIGLMKDAFQPHHHHHHHLSPHPPGTVDKKMVEKCWKLMDKVVRLCQNPKLALKNSPPYILDLLPDTYQHLRTILSRYEGKMETLGENEYFRVFMENLMKKTKQTISLFKEGKERMYEENSQPRRNLTKLSLIFSHMLAELKGIFPSGLFQGDTFRITKADAAEFWRKAFGEKTIVPWKSFRQALHEVHPISSGLEAMALKSTIDLTCNDYISVFEFDIFTRLFQPWSSLLRNWNSLAVTHPGYMAFLTYDEVKARLQKFIHKPGSYIFRLSCTRLGQWAIGYVTADGNILQTIPHNKPLFQALIDGFREGFYLFPDGRNQNPDLTGLCEPTPQDHIKVTQEQYELYCEMGSTFQLCKICAENDKDVKIEPCGHLMCTSCLTSWQESEGQGCPFCRCEIKGTEPIVVDPFDPRGSGSLLRQGAEGAPSPNYDDDDDERADDTLFMMKELAGAKVERPPSPFSMAPQASLPPVPPRLDLLPQRVCVPSSASALGTASKAASGSLHKDKPLPVPPTLRDLPPPPPPDRPYSVGAESRPQRRPLPCTPGDCPSRDKLPPVPSSRLGDSWLPRPIPKVPVSAPSSSDPWTGRELTNRHSLPFSLPSQMEPRPDVPRLGSTFSLDTSMSMNSSPLVGPECDHPKIKPSSSANAIYSLAARPLPVPKLPPGEQCEGEEDTEYMTPSSRPLRPLDTSQSSRACDCDQQIDSCTYEAMYNIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSERKAGSCQQGSGPAASAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT DIP protein P22681 original sequence version: 173 DIP protein P22681 original sequence version: 128 DIP protein P22681 original sequence version: 145 DIP protein P22681 original sequence version: 150 DIP protein P22681 original sequence version: 191 DIP protein P22681 original sequence version: 91 DIP protein P22681 original sequence version: 139 1AA6BF67377322CA gosr2_human Golgi SNAP receptor complex member 2 GOSR2 GS27 27 kDa Golgi SNARE protein Membrin protein protein human Homo sapiens Human MDPLFQQTHKQVHEIQSCMGRLETADKQSVHIVENEIQASIDQIFSRLERLEILSSKEPPNKRQNARLRVDQLKYDVQHLQTALRNFQHRRHAREQQERQREELLSRTFTTNDSDTTIPMDESLQFNSSLQKVHNGMDDLILDGHNILDGLRTQRLTLKGTQKKILDIANMLGLSNTVMRLIEKRAFQDKYFMIGGMLLTCVVMFLVVQYLT 4D5585CF858A610F rabx5_human Rab5 GDP/GTP exchange factor Rabex-5 Rabaptin-5-associated exchange factor for Rab5 RAP1 RABGEF1 RABEX5 protein protein human Homo sapiens Human MSLKSERRGIHVDQSDLLCKKGCGYYGNPAWQGFCSKCWREEYHKARQKQIQEDWELAERLQREEEEAFASSQSSQGAQSLTFSKFEEKKTNEKTRKVTTVKKFFSASSRVGSKKEIQEAKAPSPSINRQTSIETDRVSKEFIEFLKTFHKTGQEIYKQTKLFLEGMHYKRDLSIEEQSECAQDFYHNVAERMQTRGKVPPERVEKIMDQIEKYIMTRLYKYVFCPETTDDEKKDLAIQKRIRALRWVTPQMLCVPVNEDIPEVSDMVVKAITDIIEMDSKRVPRDKLACITKCSKHIFNAIKITKNEPASADDFLPTLIYIVLKGNPPRLQSNIQYITRFCNPSRLMTGEDGYYFTNLCCAVAFIEKLDAQSLNLSQEDFDRYMSGQTSPRKQEAESWSPDACLGVKQMYKNLDLLSQLNERQERIMNEAKKLEKDLIDWTDGIAREVQDIVEKYPLEIKPPNQPLAAIDSENVENDKLPPPLQPQVYAG 49ACABA276E65E91 snx6_human Sorting nexin-6 TRAF4-associated factor 2 SNX6 protein protein human Homo sapiens Human MMEGLDDGPDFLSEEDRGLKAINVDLQSDAALQVDISDALSERDKVKFTVHTKSSLPNFKQNEFSVVRQHEEFIWLHDSFVENEDYAGYIIPPAPPRPDFDASREKLQKLGEGEGSMTKEEFTKMKQELEAEYLAIFKKTVAMHEVFLCRVAAHPILRRDLNFHVFLEYNQDLSVRGKNKKEKLEDFFKNMVKSADGVIVSGVKDVDDFFEHERTFLLEYHNRVKDASAKSDRMTRSHKSAADDYNRIGSSLYALGTQDSTDICKFFLKVSELFDKTRKIEARVSADEDLKLSDLLKYYLRESQAAKDLLYRRSRSLVDYENANKALDKARAKNKDVLQAETSQQLCCQKFEKISESAKQELIDFKTRRVAAFRKNLVELAELELKHAKGNLQLLQNCLAVLNGDT DIP protein Q9UNH7 original sequence version: 120 E3659DB19C59E1BB wdfy1_human WD repeat and FYVE domain-containing protein 1 WDFY1 KIAA1435 WDF1 ZFYVE17 Phosphoinositide-binding protein 1 WD40- and FYVE domain-containing protein 1 Zinc finger FYVE domain-containing protein 17 FENS1 FYVE domain-containing protein localized to endosomes 1 protein protein human Homo sapiens Human MAAEIHSRPQSSRPVLLSKIEGHQDAVTAALLIPKEDGVITASEDRTIRVWLKRDSGQYWPSIYHTMASPCSAMAYHHDSRRIFVGQDNGAVMEFHVSEDFNKMNFIKTYPAHQNRVSAIIFSLATEWVISTGHDKCVSWMCTRSGNMLGRHFFTSWASCLQYDFDTQYAFVGDYSGQITLLKLEQNTCSVITTLKGHEGSVACLWWDPIQRLLFSGASDNSIIMWDIGGRKGRTLLLQGHHDKVQSLCYLQLTRQLVSCSSDGGIAVWNMDVSREEAPQWLESDSCQKCEQPFFWNIKQMWDTKTLGLRQHHCRKCGQAVCGKCSSKRSSYPVMGFEFQVRVCDSCYDSIKDEDRTSLATFHEGKHNISHMSMDIARGLMVTCGTDRIVKIWDMTPVVGCSLATGFSPH 25D981799D918BE3 ehd4_human EH domain-containing protein 4 Hepatocellular carcinoma-associated protein 10/11 EHD4 HCA10 HCA11 FKSG7 PAST4 PAST homolog 4 protein protein human Homo sapiens Human MFSWMGRQAGGRERAGGADAVQTVTGGLRSLYLRKVLPLEEAYRFHEFHSPALEDADFENKPMILLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMYGETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFMCSQLPNQVLKSISVIDSPGILSGEKQRISRGYDFCQVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAFRGQDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEAEAQDLFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIISYLKKEMPSVFGKENKKRELISRLPEIYIQLQREYQISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAVDNMLSNKISPLMNLISQEETSTPTQLVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAKHLIKIKLDGYELPSSLPPHLVPPSHRKSLPKAD 72DDE551829B7BF5 egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA DIP protein P00533 original sequence version: 213 DIP protein P00533 original sequence version: 214 DIP protein P00533 original sequence version: 119 DIP protein P00533 original sequence version: 207 DIP protein P00533 original sequence version: 132 DIP protein P00533-1 original sequence version: 197 DIP protein P00533 original sequence version: 163 DIP protein P00533-1 original sequence version: 159 DIP protein P00533 original sequence version: 184 DIP protein P00533-1 original sequence version: 174 DIP protein P00533-1 original sequence version: 191 DIP protein P00533 original sequence version: 218 DIP protein P00533 original sequence version: 221 DIP protein P00533-1 original sequence version: 221 DIP protein P00533 original sequence version: 226 DIP protein P00533 original sequence version: 166 DIP protein P00533-1 original sequence version: 166 DIP protein P00533 original sequence version: 194 DIP protein P00533-1 original sequence version: 194 DIP protein P00533-1 original sequence version: 224 DIP protein P00533 original sequence version: 189 DIP protein P00533-1 original sequence version: 207 DIP protein P00533 original sequence version: 177 DIP protein P00533 original sequence version: 168 D8A2A50B4EFB6ED2 tpc3l_human Trafficking protein particle complex subunit 3-like protein TRAPPC3L BET3L BET3-like protein protein protein human Homo sapiens Human MSRPAHRRPEYHKINKDLFVLTYGALVAQLCKDYEKDEDVNQYLDKMGYGIGTRLVEDFLARSCVGRCHSYSEIIDIIAQVAFKMYLGITPSVTCNNSSKNEFSLILEKNPLVEFVEELPAGRSSLCYCNLLCGIIRGALEMVHLAADVTFLQDRLKGDSVTEIGITFLKKRDEKKYRGKK 3C2604CDF74B9CAE notc2_human Neurogenic locus notch homolog protein 2 NOTCH2 protein protein human Homo sapiens Human MPALRPALLWALLALWLCCAAPAHALQCRDGYEPCVNEGMCVTYHNGTGYCKCPEGFLGEYCQHRDPCEKNRCQNGGTCVAQAMLGKATCRCASGFTGEDCQYSTSHPCFVSRPCLNGGTCHMLSRDTYECTCQVGFTGKECQWTDACLSHPCANGSTCTTVANQFSCKCLTGFTGQKCETDVNECDIPGHCQHGGTCLNLPGSYQCQCPQGFTGQYCDSLYVPCAPSPCVNGGTCRQTGDFTFECNCLPGFEGSTCERNIDDCPNHRCQNGGVCVDGVNTYNCRCPPQWTGQFCTEDVDECLLQPNACQNGGTCANRNGGYGCVCVNGWSGDDCSENIDDCAFASCTPGSTCIDRVASFSCMCPEGKAGLLCHLDDACISNPCHKGALCDTNPLNGQYICTCPQGYKGADCTEDVDECAMANSNPCEHAGKCVNTDGAFHCECLKGYAGPRCEMDINECHSDPCQNDATCLDKIGGFTCLCMPGFKGVHCELEINECQSNPCVNNGQCVDKVNRFQCLCPPGFTGPVCQIDIDDCSSTPCLNGAKCIDHPNGYECQCATGFTGVLCEENIDNCDPDPCHHGQCQDGIDSYTCICNPGYMGAICSDQIDECYSSPCLNDGRCIDLVNGYQCNCQPGTSGVNCEINFDDCASNPCIHGICMDGINRYSCVCSPGFTGQRCNIDIDECASNPCRKGATCINGVNGFRCICPEGPHHPSCYSQVNECLSNPCIHGNCTGGLSGYKCLCDAGWVGINCEVDKNECLSNPCQNGGTCDNLVNGYRCTCKKGFKGYNCQVNIDECASNPCLNQGTCFDDISGYTCHCVLPYTGKNCQTVLAPCSPNPCENAAVCKESPNFESYTCLCAPGWQGQRCTIDIDECISKPCMNHGLCHNTQGSYMCECPPGFSGMDCEEDIDDCLANPCQNGGSCMDGVNTFSCLCLPGFTGDKCQTDMNECLSEPCKNGGTCSDYVNSYTCKCQAGFDGVHCENNINECTESSCFNGGTCVDGINSFSCLCPVGFTGSFCLHEINECSSHPCLNEGTCVDGLGTYRCSCPLGYTGKNCQTLVNLCSRSPCKNKGTCVQKKAESQCLCPSGWAGAYCDVPNVSCDIAASRRGVLVEHLCQHSGVCINAGNTHYCQCPLGYTGSYCEEQLDECASNPCQHGATCSDFIGGYRCECVPGYQGVNCEYEVDECQNQPCQNGGTCIDLVNHFKCSCPPGTRGLLCEENIDDCARGPHCLNGGQCMDRIGGYSCRCLPGFAGERCEGDINECLSNPCSSEGSLDCIQLTNDYLCVCRSAFTGRHCETFVDVCPQMPCLNGGTCAVASNMPDGFICRCPPGFSGARCQSSCGQVKCRKGEQCVHTASGPRCFCPSPRDCESGCASSPCQHGGSCHPQRQPPYYSCQCAPPFSGSRCELYTAPPSTPPATCLSQYCADKARDGVCDEACNSHACQWDGGDCSLTMENPWANCSSPLPCWDYINNQCDELCNTVECLFDNFECQGNSKTCKYDKYCADHFKDNHCDQGCNSEECGWDGLDCAADQPENLAEGTLVIVVLMPPEQLLQDARSFLRALGTLLHTNLRIKRDSQGELMVYPYYGEKSAAMKKQRMTRRSLPGEQEQEVAGSKVFLEIDNRQCVQDSDHCFKNTDAAAALLASHAIQGTLSYPLVSVVSESLTPERTQLLYLLAVAVVIILFIILLGVIMAKRKRKHGSLWLPEGFTLRRDASNHKRREPVGQDAVGLKNLSVQVSEANLIGTGTSEHWVDDEGPQPKKVKAEDEALLSEEDDPIDRRPWTQQHLEAADIRRTPSLALTPPQAEQEVDVLDVNVRGPDGCTPLMLASLRGGSSDLSDEDEDAEDSSANIITDLVYQGASLQAQTDRTGEMALHLAARYSRADAAKRLLDAGADANAQDNMGRCPLHAAVAADAQGVFQILIRNRVTDLDARMNDGTTPLILAARLAVEGMVAELINCQADVNAVDDHGKSALHWAAAVNNVEATLLLLKNGANRDMQDNKEETPLFLAAREGSYEAAKILLDHFANRDITDHMDRLPRDVARDRMHHDIVRLLDEYNVTPSPPGTVLTSALSPVICGPNRSFLSLKHTPMGKKSRRPSAKSTMPTSLPNLAKEAKDAKGSRRKKSLSEKVQLSESSVTLSPVDSLESPHTYVSDTTSSPMITSPGILQASPNPMLATAAPPAPVHAQHALSFSNLHEMQPLAHGASTVLPSVSQLLSHHHIVSPGSGSAGSLSRLHPVPVPADWMNRMEVNETQYNEMFGMVLAPAEGTHPGIAPQSRPPEGKHITTPREPLPPIVTFQLIPKGSIAQPAGAPQPQSTCPPAVAGPLPTMYQIPEMARLPSVAFPTAMMPQQDGQVAQTILPAYHPFPASVGKYPTPPSQHSYASSNAAERTPSHSGHLQGEHPYLTPSPESPDQWSSSSPHSASDWSDVTTSPTPGGAGGGQRGPGTHMSEPPHNNMQVYA 605B1B963C812BE1 arfg2_human ADP-ribosylation factor GTPase-activating protein 2 ARFGAP2 GTPase-activating protein ZNF289 ZNF289 Zinc finger protein 289 Nbla10535 protein protein human Homo sapiens Human MAAEPNKTEIQTLFKRLRAVPTNKACFDCGAKNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDSNWNWFQLRCMQVGGNANATAFFRQHGCTANDANTKYNSRAAQMYREKIRQLGSAALARHGTDLWIDNMSSAVPNHSPEKKDSDFFTEHTQPPAWDAPATEPSGTQQPAPSTESSGLAQPEHGPNTDLLGTSPKASLELKSSIIGKKKPAAAKKGLGAKKGLGAQKVSSQSFSEIERQAQVAEKLREQQAADAKKQAEESMVASMRLAYQELQIDRKKEEKKLQNLEGKKREQAERLGMGLVSRSSVSHSVLSEMQVIEQETPVSAKSSRSQLDLFDDVGTFASGPPKYKDNPFSLGESFGSRWDTDAAWGMDRVEEKEPEVTISSIRPISERATNRREVESRSSGLESSEARQKFAGAKAISSDMFFGREVDAEYEARSRLQQLSGSSAISSSDLFGDMDGAHGAGSVSLGNVLPTADIAQFKQGVKSVAGKMAVLANGVMNSLQDRYGSY 16655EBA94D29E81 vps29_human Vacuolar protein sorting-associated protein 29 Vesicle protein sorting 29 PEP11 homolog VPS29 DC7 DC15 MDS007 protein protein human Homo sapiens Human MLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKESYDYLKTLAGDVHIVRGDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQFDVDILISGHTHKFEAFEHENKFYINPGSATGAYNALETNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYKKP DIP protein Q9UBQ0 original sequence version: 51 DIP protein Q9UBQ0-1 original sequence version: 117 DIP protein Q9UBQ0 original sequence version: 117 DIP protein Q9UBQ0 original sequence version: 129 6E0CDE6B720C9BF8 ap2a1_human AP-2 complex subunit alpha-1 AP2A1 ADTAA CLAPA1 Adaptor protein complex AP-2 subunit alpha-1 Alpha1-adaptin Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit Alpha-adaptin A Clathrin assembly protein complex 2 alpha-A large chain 100 kDa coated vesicle protein A Adaptor-related protein complex 2 subunit alpha-1 protein protein human Homo sapiens Human MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAGSALDDGRRDPSSNDINGGMEPTPSTVSTPSPSADLLGLRAAPPPAAPPASAGAGNLLVDVFDGPAAQPSLGPTPEEAFLSELEPPAPESPMALLADPAPAADPGPEDIGPPIPEADELLNKFVCKNNGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQNFSPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGAPQALTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPQQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF DIP protein O95782 original sequence version: 149 D9FB569E7EDDF6ED iqga2_human Ras GTPase-activating-like protein IQGAP2 IQGAP2 protein protein human Homo sapiens Human MPHEELPSLQRPRYGSIVDDERLSAEEMDERRRQNIAYEYLCHLEEAKRWMEVCLVEELPPTTELEEGLRNGVYLAKLAKFFAPKMVSEKKIYDVEQTRYKKSGLHFRHTDNTVQWLRAMESIGLPKIFYPETTDVYDRKNIPRMIYCIHALSLYLFKLGIAPQIQDLLGKVDFTEEEISNMRKELEKYGIQMPSFSKIGGILANELSVDEAALHAAVIAINEAVEKGIAEQTVVTLRNPNAVLTLVDDNLAPEYQKELWDAKKKKEENARLKNSCISEEERDAYEELLTQAEIQGNINKVNRQAAVDHINAVIPEGDPENTLLALKKPEAQLPAVYPFAAAMYQNELFNLQKQNTMNYLAHEELLIAVEMLSAVALLNQALESNDLVSVQNQLRSPAIGLNNLDKAYVERYANTLLSVKLEVLSQGQDNLSWNEIQNCIDMVNAQIQEENDRVVAVGYINEAIDEGNPLRTLETLLLPTANISDVDPAHAQHYQDVLYHAKSQKLGDSESVSKVLWLDEIQQAVDDANVDKDRAKQWVTLVVDVNQCLEGKKSSDILSVLKSSTSNANDIIPECADKYYDALVKAKELKSERVSSDGSWLKLNLHKKYDYYYNTDSKESSWVTPESCLYKESWLTGKEIEDIIEEVTVGYIRENIWSASEELLLRFQATSSGPILREEFEARKSFLHEQEENVVKIQAFWKGYKQRKEYMHRRQTFIDNTDSIVKIQSWFRMATARKSYLSRLQYFRDHNNEIVKIQSLLRANKARDDYKTLVGSENPPLTVIRKFVYLLDQSDLDFQEELEVARLREEVVTKIRANQQLEKDLNLMDIKIGLLVKNRITLEDVISHSKKLNKKKGGEMEILNNTDNQGIKSLSKERRKTLETYQQLFYLLQTNPLYLAKLIFQMPQNKSTKFMDTVIFTLYNYASNQREEYLLLKLFKTALEEEIKSKVDQVQDIVTGNPTVIKMVVSFNRGARGQNTLRQLLAPVVKEIIDDKSLIINTNPVEVYKAWVNQLETQTGEASKLPYDVTTEQALTYPEVKNKLEASIENLRRVTDKVLNSIISSLDLLPYGLRYIAKVLKNSIHEKFPDATEDELLKIVGNLLYYRYMNPAIVAPDGFDIIDMTAGGQINSDQRRNLGSVAKVLQHAASNKLFEGENEHLSSMNNYLSETYQEFRKYFKEACNVPEPEEKFNMDKYTDLVTVSKPVIYISIEEIISTHSLLLEHQDAIAPEKNDLLSELLGSLGEVPTVESFLGEGAVDPNDPNKANTLSQLSKTEISLVLTSKYDIEDGEAIDSRSLMIKTKKLIIDVIRNQPGNTLTEILETPATAQQEVDHATDMVSRAMIDSRTPEEMKHSQSMIEDAQLPLEQKKRKIQRNLRTLEQTGHVSSENKYQDILNEIAKDIRNQRIYRKLRKAELAKLQQTLNALNKKAAFYEEQINYYDTYIKTCLDNLKRKNTRRSIKLDGKGEPKGAKRAKPVKYTAAKLHEKGVLLDIDDLQTNQFKNVTFDIIATEDVGIFDVRSKFLGVEMEKVQLNIQDLLQMQYEGVAVMKMFDKVKVNVNLLIYLLNKKFYGK BCC8CED6645CB09B rab19_human Ras-related protein Rab-19 RAB19 RAB19B protein protein human Homo sapiens Human MHFSSSARAADENFDYLFKIILIGDSNVGKTCVVQHFKSGVYTETQQNTIGVDFTVRSLDIDGKKVKMQVWDTAGQERFRTITQSYYRSAHAAIIAYDLTRRSTFESIPHWIHEIEKYGAANVVIMLIGNKCDLWEKRHVLFEDACTLAEKYGLLAVLETSAKESKNIEEVFVLMAKELIARNSLHLYGESALNGLPLDSSPVLMAQGPSEKTHCTC 787695528A6EE366 cld3_human Claudin-3 CLDN3 C7orf1 CPETR2 Clostridium perfringens enterotoxin receptor 2 Rat ventral prostate.1 protein homolog protein protein human Homo sapiens Human MSMGLEITGTALAVLGWLGTIVCCALPMWRVSAFIGSNIITSQNIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALIVVAILLAAFGLLVALVGAQCTNCVQDDTAKAKITIVAGVLFLLAALLTLVPVSWSANTIIRDFYNPVVPEAQKREMGAGLYVGWAAAALQLLGGALLCCSCPPREKKYTATKVVYSAPRSTGPGASLGTGYDRKDYV DIP protein O15551 original sequence version: 119 1C826EFFF1563C56 rab26_human Ras-related protein Rab-26 RAB26 protein protein human Homo sapiens Human MSRKKTPKSKGASTPAASTLPTANGARPARSGTALSGPDAPPNGPLQPGRPSLGGGVDFYDVAFKVMLVGDSGVGKTCLLVRFKDGAFLAGTFISTVGIDFRNKVLDVDGVKVKLQMWDTAGQERFRSVTHAYYRDAHALLLLYDVTNKASFDNIQAWLTEIHEYAQHDVALMLLGNKVDSAHERVVKREDGEKLAKEYGLPFMETSAKTGLNVDLAFTAIAKELKQRSMKAPSEPRFRLHDYVKREGRGASCCRP 575232F4B9E7FA7E fgd4_human FYVE, RhoGEF and PH domain-containing protein 4 FGD4 FRABP ZFYVE6 Actin filament-binding protein frabin FGD1-related F-actin-binding protein Zinc finger FYVE domain-containing protein 6 protein protein human Homo sapiens Human MEEIKPASASCVSKEKPSKVSDLISRFEGGSSLSNYSDLKKESAVNLNAPRTPGRHGLTTTPQQKLLSQHLPQRQGNDTDKTQGAQTCVANGVMAAQNQMECEEEKAATLSSDTSIQASEPLLDTHIVNGERDETATAPASPTTDSCDGNASDSSYRTPGIGPVLPLEERGAETETKVQERENGESPLELEQLDQHHEMKETNEQKLHKIANELLLTERAYVNRLDLLDQVFYCKLLEEANRGSFPAEMVNKIFSNISSINAFHSKFLLPELEKRMQEWETTPRIGDILQKLAPFLKMYGEYVKGFDNAMELVKNMTERIPQFKSVVEEIQKQKICGSLTLQHHMLEPVQRIPRYEMLLKDYLRKLPPDSLDWNDAKKSLEIISTAASHSNSAIRKMENLKKLLEIYEMLGEEEDIVNPSNELIKEGQILKLAARNTSAQERYLFLFNNMLLYCVPKFSLVGSKFTVRTRVGIDGMKIVETQNEEYPHTFQVSGKERTLELQASSAQDKEEWIKALQETIDAFHQRHETFRNAIAKDNDIHSEVSTAELGKRAPRWIRDNEVTMCMKCKEPFNALTRRRHHCRACGYVVCWKCSDYKAQLEYDGGKLSKVCKDCYQIISGFTDSEEKKRKGILEIESAEVSGNSVVCSFLQYMEKSKPWQKAWCVIPKQDPLVLYMYGAPQDVRAQATIPLLGYVVDEMPRSADLPHSFKLTQSKSVHSFAADSEELKQKWLKVILLAVTGETPGGPNEHPATLDDHPEPKKKSEC B919A7C1D164B05D tpc6b_human Trafficking protein particle complex subunit 6B TRAPPC6B protein protein human Homo sapiens Human MADEALFLLLHNEMVSGVYKSAEQGEVENGRCITKLENMGFRVGQGLIERFTKDTARFKDELDIMKFICKDFWTTVFKKQIDNLRTNHQGIYVLQDNKFRLLTQMSAGKQYLEHASKYLAFTCGLIRGGLSNLGIKSIVTAEVSSMPACKFQVMIQKL DIP protein Q86SZ2 original sequence version: 108 5A8B0474548C304B rb11a_human Ras-related protein Rab-11A YL8 RAB11A RAB11 protein protein human Homo sapiens Human MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI DIP protein P62491 original sequence version: 38 DIP protein P62491-1 original sequence version: 132 DIP protein P62491 original sequence version: 122 DIP protein P62491 original sequence version: 89 DIP protein P62491-1 original sequence version: 137 DIP protein P62491 original sequence version: 136 DIP protein P62491 original sequence version: 137 DIP protein P62491-1 original sequence version: 140 76FC1E113A29B269 golm1_human Golgi membrane protein 1 Golgi phosphoprotein 2 Golgi membrane protein GP73 GOLM1 PSEC0242 UNQ686/PRO1326 C9orf155 GOLPH2 protein protein human Homo sapiens Human MMGLGNGRRSMKSPPLVLAALVACIIVLGFNYWIASSRSVDLQTRIMELEGRVRRAAAERGAVELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL 24C1DC31E47B5B77 erbb4_human Receptor tyrosine-protein kinase erbB-4 ERBB4 HER4 p180erbB4 Proto-oncogene-like protein c-ErbB-4 Tyrosine kinase-type cell surface receptor HER4 protein protein human Homo sapiens Human MKPATGLWVWVSLLVAAGTVQPSDSQSVCAGTENKLSSLSDLEQQYRALRKYYENCEVVMGNLEITSIEHNRDLSFLRSVREVTGYVLVALNQFRYLPLENLRIIRGTKLYEDRYALAIFLNYRKDGNFGLQELGLKNLTEILNGGVYVDQNKFLCYADTIHWQDIVRNPWPSNLTLVSTNGSSGCGRCHKSCTGRCWGPTENHCQTLTRTVCAEQCDGRCYGPYVSDCCHRECAGGCSGPKDTDCFACMNFNDSGACVTQCPQTFVYNPTTFQLEHNFNAKYTYGAFCVKKCPHNFVVDSSSCVRACPSSKMEVEENGIKMCKPCTDICPKACDGIGTGSLMSAQTVDSSNIDKFINCTKINGNLIFLVTGIHGDPYNAIEAIDPEKLNVFRTVREITGFLNIQSWPPNMTDFSVFSNLVTIGGRVLYSGLSLLILKQQGITSLQFQSLKEISAGNIYITDNSNLCYYHTINWTTLFSTINQRIVIRDNRKAENCTAEGMVCNHLCSSDGCWGPGPDQCLSCRRFSRGRICIESCNLYDGEFREFENGSICVECDPQCEKMEDGLLTCHGPGPDNCTKCSHFKDGPNCVEKCPDGLQGANSFIFKYADPDRECHPCHPNCTQGCNGPTSHDCIYYPWTGHSTLPQHARTPLIAAGVIGGLFILVIVGLTFAVYVRRKSIKKKRALRRFLETELVEPLTPSGTAPNQAQLRILKETELKRVKVLGSGAFGTVYKGIWVPEGETVKIPVAIKILNETTGPKANVEFMDEALIMASMDHPHLVRLLGVCLSPTIQLVTQLMPHGCLLEYVHEHKDNIGSQLLLNWCVQIAKGMMYLEERRLVHRDLAARNVLVKSPNHVKITDFGLARLLEGDEKEYNADGGKMPIKWMALECIHYRKFTHQSDVWSYGVTIWELMTFGGKPYDGIPTREIPDLLEKGERLPQPPICTIDVYMVMVKCWMIDADSRPKFKELAAEFSRMARDPQRYLVIQGDDRMKLPSPNDSKFFQNLLDEEDLEDMMDAEEYLVPQAFNIPPPIYTSRARIDSNRSEIGHSPPPAYTPMSGNQFVYRDGGFAAEQGVSVPYRAPTSTIPEAPVAQGATAEIFDDSCCNGTLRKPVAPHVQEDSSTQRYSADPTVFAPERSPRGELDEEGYMTPMRDKPKQEYLNPVEENPFVSRRKNGDLQALDNPEYHNASNGPPKAEDEYVNEPLYLNTFANTLGKAEYLKNNILSMPEKAKKAFDNPDYWNHSLPPRSTLQHPDYLQEYSTKYFYKQNGRIRPIVAENPEYLSEFSLKPGTVLPPPPYRHRNTVV DIP protein Q15303 original sequence version: 163 DIP protein Q15303-1 original sequence version: 92 DIP protein Q15303-1 original sequence version: 127 DIP protein Q15303-1 original sequence version: 96 DIP protein Q15303 original sequence version: 96 5E4AE80985D88761 rtp2_human Receptor-transporting protein 2 3CxxC-type zinc finger protein 2 Z3CXXC2 RTP2 protein protein human Homo sapiens Human MCTSLTTCEWKKVFYEKMEVAKPADSWELIIDPNLKPSELAPGWKQYLEQHASGRFHCSWCWHTWQSAHVVILFHMFLDRAQRAGSVRMRVFKQLCYECGTARLDESSMLEENIEGLVDNLITSLREQCYEEDGGQYRIHVASRPDSGPHRAEFCEACQEGIVHWKPSEKLLEEEVTTYTSEASKPRAQAGSGYNFLSLRWCLFWASLCLLVVYLQFSFLSPAFF 62F8ADA80DC90A73 rab3d_human Ras-related protein Rab-3D RAB3D GOV RAB16 protein protein human Homo sapiens Human MASAGDTQAGPRDAADQNFDYMFKLLLIGNSSVGKTSFLFRYADDSFTPAFVSTVGIDFKVKTVYRHDKRIKLQIWDTAGQERYRTITTAYYRGAMGFLLMYDIANQESFAAVQDWATQIKTYSWDNAQVILVGNKCDLEDERVVPAEDGRRLADDLGFEFFEASAKENINVKQVFERLVDVICEKMNESLEPSSSSGSNGKGPAVGDAPAPQPSSCSC 714754826C405EA7 gdia_human Rab GDP dissociation inhibitor alpha GDI1 OPHN2 RABGDIA XAP4 Guanosine diphosphate dissociation inhibitor 1 Oligophrenin-2 Protein XAP-4 GDIL protein protein human Homo sapiens Human MDEEYDVIVLGTGLTECILSGIMSVNGKKVLHMDRNPYYGGESSSITPLEELYKRFQLLEGPPESMGRGRDWNVDLIPKFLMANGQLVKMLLYTEVTRYLDFKVVEGSFVYKGGKIYKVPSTETEALASNLMGMFEKRRFRKFLVFVANFDENDPKTFEGVDPQTTSMRDVYRKFDLGQDVIDFTGHALALYRTDDYLDQPCLETVNRIKLYSESLARYGKSPYLYPLYGLGELPQGFARLSAIYGGTYMLNKPVDDIIMENGKVVGVKSEGEVARCKQLICDPSYIPDRVRKAGQVIRIICILSHPIKNTNDANSCQIIIPQNQVNRKSDIYVCMISYAHNVAAQGKYIAIASTTVETTDPEKEVEPALELLEPIDQKFVAISDLYEPIDDGCESQVFCSCSYDATTHFETTCNDIKDIYKRMAGTAFDFENMKRKQNDVFGEAEQ BC283A445E50A652 ap3s1_human AP-3 complex subunit sigma-1 AP3S1 Sigma-3A-adaptin Sigma-adaptin 3a CLAPS3 AP-3 complex subunit sigma-3A Adaptor-related protein complex 3 subunit sigma-1 Clathrin-associated/assembly/adaptor protein, small 3 protein protein human Homo sapiens Human MIKAILIFNNHGKPRLSKFYQPYSEDTQQQIIRETFHLVSKRDENVCNFLEGGLLIGGSDNKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHVDKVHNILAEMVMGGMVLETNMNEIVTQIDAQNKLEKSEAGLAGAPARAVSAVKNMNLPEIPRNINIGDISIKVPNLPSFK 1EF17F375881288D 235rw34-1 11 20 unspecified role unspecified role bait bait 33 unspecified role unspecified role prey prey physical association physical association 3E egfr-golm1-1 2 20 unspecified role unspecified role neutral component neutral component 33 unspecified role unspecified role neutral component neutral component colocalization colocalization 4B There is co-localization of GOLM1 with EGFR in endosome-like structures. egfr-golm1-2 4 20 unspecified role unspecified role neutral component neutral component 33 unspecified role unspecified role neutral component neutral component tmd required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 4 B/E, 7C, Supp 4E There is co-localization of GOLM1 with EGFR in endosome-like structures. EGF:- Maximum signal intensity was reached 15–30 min after EGF stimulation. It has been assumed that the same cell line was used in Supp. fig 4E as Figure 4E. egfr-golm1-3 12 20 unspecified role unspecified role neutral component neutral component c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 33 unspecified role unspecified role neutral component neutral component c_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 4C, Movie S1 EGF -the interaction between GOLM1 and EGFR reached maximum strength in 15–30 min after EGF stimulation, then decreased slowly over time. egfr-golm1-4 7 20 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait tmd binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8NBJ4:p.Ser10_Lys12delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain SMK AAA Tue Jan 24 14:02:21 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser_met_lys10-12_ala_ala_ala' Wed May 02 16:26:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser_met_lys10-12ala_ala_ala' Tue Jun 30 19:12:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser10_Lys12delinsAlaAlaAla' Q8NBJ4:p.Gly7_Arg9delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain GRR AAA Wed May 02 16:24:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg7-9ala_ala_ala' Tue Jun 30 19:25:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly7_Arg9delinsAlaAlaAla' Q8NBJ4:p.Gly5_Asn6delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain GN AA Wed May 02 17:09:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_asn5-6ala_ala' Tue Jun 30 19:16:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly5_Asn6delinsAlaAla' Q8NBJ4:p.Gly7Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Wed May 02 17:17:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly7ala' Tue Jun 30 20:24:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly7Ala' Q8NBJ4:p.Arg8Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:42:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg8ala' Tue Jun 30 18:57:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg8Ala' Q8NBJ4:p.Arg9Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:30:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg9ala' Tue Jun 30 20:01:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg9Ala' Q8NBJ4:p.Ser10Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Wed May 02 20:11:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser10ala' Tue Jun 30 20:05:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser10Ala' Q8NBJ4:p.Met11Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Wed May 02 14:57:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met11ala' Tue Jun 30 18:55:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met11Ala' Q8NBJ4:p.Lys12Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 20:30:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys12ala' Tue Jun 30 16:58:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys12Ala' Q8NBJ4:p.[Gly7_Arg9delinsAlaAlaAla;Met11_Lys12delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain GRR AAA certain certain sequence position certain certain certain sequence position certain MK AA Tue Jan 24 13:52:22 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg7-9ala_ala_ala, met_lys11-12ala_ala' Wed May 02 16:06:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg7-9ala_ala_ala,met_lys11-12ala_ala' Tue Jun 30 19:25:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly7_Arg9delinsAlaAlaAla;Met11_Lys12delinsAlaAla]' Q8NBJ4:p.Gly7_Lys12delinsAlaAlaAlaAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain GRRSMK AAAAAA Wed May 02 15:59:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg_ser_met_lys7-12ala_ala_ala_ala_ala_ala' Tue Jun 30 17:03:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly7_Lys12delinsAlaAlaAlaAlaAlaAla' physical association physical association 4D, 5E/F, 7D EGF:- Maximum signal intensity was reached 15–30 min after EGF stimulation. egfr-golm1-5 11 32 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait physical association physical association 6G Knocking-down GOLM1 expression reduces the strength of this interaction. 4356454-1 7 37 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Supp Fig 5B golm1-egfr-1 10 20 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait tmd binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8NBJ4:p.Ser10_Lys12delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain SMK AAA Tue Jan 24 13:48:02 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser_met_lys10-12_ala_ala_ala' Wed May 02 17:17:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser_met_lys10-12ala_ala_ala' Tue Jun 30 16:24:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser10_Lys12delinsAlaAlaAla' Q8NBJ4:p.Gly7_Arg9delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain GRR AAA Wed May 02 14:40:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg7-9ala_ala_ala' Tue Jun 30 16:22:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly7_Arg9delinsAlaAlaAla' Q8NBJ4:p.Gly5_Asn6delinsAlaAla mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain GN AA Wed May 02 17:08:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_asn5-6ala_ala' Tue Jun 30 18:42:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly5_Asn6delinsAlaAla' Q8NBJ4:p.Gly7Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G A Wed May 02 15:57:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly7ala' Tue Jun 30 18:35:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly7Ala' Q8NBJ4:p.Arg8Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:10:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg8ala' Tue Jun 30 16:03:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg8Ala' Q8NBJ4:p.Arg9Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:56:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg9ala' Tue Jun 30 17:21:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg9Ala' Q8NBJ4:p.Ser10Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:27:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser10ala' Tue Jun 30 20:13:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser10Ala' Q8NBJ4:p.Met11Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Wed May 02 16:32:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met11ala' Tue Jun 30 18:53:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met11Ala' Q8NBJ4:p.Lys12Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:14:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys12ala' Tue Jun 30 19:27:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys12Ala' Q8NBJ4:p.[Gly7_Arg9delinsAlaAlaAla;Met11_Lys12delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain GRR AAA certain certain sequence position certain certain certain sequence position certain MK AA Tue Jan 24 13:59:40 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg7-9ala_ala_ala, met_lys11-12ala_ala' Wed May 02 20:18:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg7-9ala_ala_ala,met_lys11-12ala_ala' Tue Jun 30 20:17:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly7_Arg9delinsAlaAlaAla;Met11_Lys12delinsAlaAla]' Q8NBJ4:p.Gly7_Lys12delinsAlaAlaAlaAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain GRRSMK AAAAAA Wed May 02 19:50:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg_ser_met_lys7-12ala_ala_ala_ala_ala_ala' Tue Jun 30 17:53:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly7_Lys12delinsAlaAlaAlaAlaAlaAla' physical association physical association Supp 7 B/D 45ta4wtw-1 3 20 unspecified role unspecified role prey prey purified purified region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 33 unspecified role unspecified role bait bait purified purified cytoplasmic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q8NBJ4:p.[Gly7_Arg9delinsAlaAlaAla;Met11_Lys12delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain GRR AAA certain certain sequence position certain certain certain sequence position certain MK AA Tue Jan 24 13:49:59 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg7-9ala_ala_ala, met_lys11-12ala_ala' Wed May 02 16:10:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_arg_arg7-9ala_ala_ala,met_lys11-12ala_ala' Tue Jun 30 18:30:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gly7_Arg9delinsAlaAlaAla;Met11_Lys12delinsAlaAla]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp 7E 23356qa6swe-1 5 36 unspecified role unspecified role prey prey 34 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 35 unspecified role unspecified role prey prey 38 unspecified role unspecified role prey prey 31 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 30 unspecified role unspecified role prey prey 37 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association 3A, Supp Table 5 3242-1 9 20 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3C egfr-golm1-6 6 20 unspecified role unspecified role bait bait 33 unspecified role unspecified role prey prey c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3C egfr-golm1-7 8 33 unspecified role unspecified role prey prey c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 20 unspecified role unspecified role bait bait physical association physical association 3D golm1-egfr-2 7 20 unspecified role unspecified role prey prey 33 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3D golm1-rab11a-1 1 32 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 33 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 6H I have assumed that Rab11A was used