IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. Cancer cell 2016 imex curation Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2016-NOV-08 AT 09:35 GMT AT 09:35 GMT by ORCHARD Cancer cell 2016 imex curation PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. Cancer cell 2016 imex curation Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions human-hcc-1954 HCC-1954 Ductal Carcinoma cell line hcc-1954 HCC-1954 Human Breast (Ductal) Carcinoma cell line ATCC Number = CRL-2338 HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases. ER-, PR-, ERBB2+. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2016-NOV-08 AT 09:35 GMT AT 09:35 GMT by ORCHARD Cancer cell 2016 imex curation PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. Cancer cell 2016 imex curation Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. shPDK1 or control transduced cells, treated with/without BYL719 (a PI3K Inhibitor) and/or GSK2334470, a novel PDK1 inhibitor . pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2016-NOV-08 AT 09:35 GMT AT 09:35 GMT by ORCHARD Cancer cell 2016 imex curation PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. Cancer cell 2016 imex curation Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions human-jimt-1 JIMT-1-ductal breast carcinoma, HER2 amplified, trastuzumab insensitive. Human jimt-1 ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2016-NOV-08 AT 09:35 GMT AT 09:35 GMT by ORCHARD Cancer cell 2016 imex curation PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. Cancer cell 2016 imex curation Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2016-NOV-08 AT 09:35 GMT AT 09:35 GMT by ORCHARD Cancer cell 2016 imex curation PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. PDK1-SGK1 Signaling Sustains AKT-Independent mTORC1 Activation and Confers Resistance to PI3Kα Inhibition. Cancer cell 2016 imex curation Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions human-jimt-1 JIMT-1-ductal breast carcinoma, HER2 amplified, trastuzumab insensitive. Human jimt-1 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Castel P., Ellis H., Bago R., Toska E., Razavi P., Carmona FJ., Kannan S., Verma CS., Dickler M., Chandarlapaty S., Brogi E., Alessi DR., Baselga J., Scaltriti M. Cancer - Interactions investigated in the context of cancer I have not added the Kinase assays as an IP-ed protein (no purification) is used in all instances I can see. The full gels are not shown. Sucrose gradient 'fugation is using cell lysate - not added. pLPCX-HA-SGK1(delta60) was determined to be of Human origin:pmid:11278764 (2001) scaltrim@mskcc.org,baselgaj@mskcc.org Accepted 2016-NOV-08 AT 09:35 GMT AT 09:35 GMT by ORCHARD Cancer cell 2016 imex curation irs2_human_gene Insulin receptor substrate 2 IRS2 gene gene human Homo sapiens Human foxo3_human Forkhead box protein O3 FOXO3 FKHRL1 FOXO3A Forkhead in rhabdomyosarcoma-like 1 AF6q21 protein protein protein human Homo sapiens Human MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG E5B4E830665A9982 DIP protein O43524 original sequence version: 82 E5B4E830665A9982 mtor_human Serine/threonine-protein kinase mTOR MTOR FRAP FRAP2 FK506-binding protein 12-rapamycin complex-associated protein 1 Rapamycin target protein 1 FRAP1 Mammalian target of rapamycin FKBP12-rapamycin complex-associated protein Mechanistic target of rapamycin RAFT1 RAPT1 Rapamycin and FKBP12 target 1 protein protein human Homo sapiens Human MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENLCQCYIGWCPFW DIP protein P42345 original sequence version: 158 DIP protein P42345 original sequence version: 82 DIP protein P42345 original sequence version: 108 DIP protein P42345 original sequence version: 107 DIP protein P42345 original sequence version: 138 DIP protein P42345 original sequence version: 168 DIP protein P42345 original sequence version: 173 DIP protein P42345 original sequence version: 120 DIP protein P42345 original sequence version: 149 DIP protein P42345 original sequence version: 134 DIP protein P42345 original sequence version: 148 DIP protein P42345 original sequence version: 118 7D9AD6E784882AB4 7-methyl-gtp 7-methyl-GTP small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) tnf10_human_gene Tumor necrosis factor ligand superfamily member 10 TNFSF10 APO2L TRAIL gene gene human Homo sapiens Human 4ebp1_human Eukaryotic translation initiation factor 4E-binding protein 1 Phosphorylated heat- and acid-stable protein regulated by insulin 1 EIF4EBP1 protein protein human Homo sapiens Human MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI DIP protein Q13541 original sequence version: 96 DIP protein Q13541 original sequence version: 103 DIP protein Q13541 original sequence version: 120 DIP protein Q13541 original sequence version: 113 1682A6BA74132966 sgk1_human_gene Serine/threonine-protein kinase Sgk1 SGK1 SGK gene gene human Homo sapiens Human sgk1_human Serine/threonine-protein kinase Sgk1 SGK1 SGK Serum/glucocorticoid-regulated kinase 1 protein protein human Homo sapiens Human MTVKTEAAKGTLTYSRMRGMVAILIAFMKQRRMGLNDFIQKIANNSYACKHPEVQSILKISQPQEPELMNANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAEEVFYAVKVLQKKAILKKKEEKHIMSERNVLLKNVKHPFLVGLHFSFQTADKLYFVLDYINGGELFYHLQRERCFLEPRARFYAAEIASALGYLHSLNIVYRDLKPENILLDSQGHIVLTDFGLCKENIEHNSTTSTFCGTPEYLAPEVLHKQPYDRTVDWWCLGAVLYEMLYGLPPFYSRNTAEMYDNILNKPLQLKPNITNSARHLLEGLLQKDRTKRLGAKDDFMEIKSHVFFSLINWDDLINKKITPPFNPNVSGPNDLRHFDPEFTEEPVPNSIGKSPDSVLVTASVKEAAEAFLGFSYAPPTDSFL DIP protein O00141 original sequence version: 145 F3697C63AB1F499D tsc2_human Tuberin TSC2 Tuberous sclerosis 2 protein TSC4 protein protein human Homo sapiens Human MAKPTSKDSGLKEKFKILLGLGTPRPNPRSAEGKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPERPLEARHAVLALLKAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRHITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASSVDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSAIYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNEVVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVEELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERFFRSESRGAVRIKVLDVLSFVLLINRQFYEEELINSVVISQLSHIPEDKDHQVRKLATQLLVDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQTKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADSLHRLGLPNKDGVVRFSPYCVCDYMEPERGSEKKTSGPLSPPTGPPGPAPAGPAVRLGSVPYSLLFRVLLQCLKQESDWKVLKLVLGRLPESLRYKVLIFTSPCSVDQLCSALCSMLSGPKTLERLRGAPEGFSRTDLHLAVVPVLTALISYHNYLDKTKQREMVYCLEQGLIHRCASQCVVALSICSVEMPDIIIKALPVLVVKLTHISATASMAVPLLEFLSTLARLPHLYRNFAAEQYASVFAISLPYTNPSKFNQYIVCLAHHVIAMWFIRCRLPFRKDFVPFITKGLRSNVLLSFDDTPEKDSFRARSTSLNERPKSLRIARPPKQGLNNSPPVKEFKESSAAEAFRCRSISVSEHVVRSRIQTSLTSASLGSADENSVAQADDSLKNLHLELTETCLDMMARYVFSNFTAVPKRSPVGEFLLAGGRTKTWLVGNKLVTVTTSVGTGTRSLLGLDSGELQSGPESSSSPGVHVRQTKEAPAKLESQAGQQVSRGARDRVRSMSGGHGLRVGALDVPASQFLGSATSPGPRTAPAAKPEKASAGTRVPVQEKTNLAAYVPLLTQGWAEILVRRPTGNTSWLMSLENPLSPFSSDINNMPLQELSNALMAAERFKEHRDTALYKSLSVPAASTAKPPPLPRSNTVASFSSLYQSSCQGQLHRSVSWADSAVVMEEGSPGEVPVLVEPPGLEDVEAALGMDRRTDAYSRSSSVSSQEEKSLHAEELVGRGIPIERVVSSEGGRPSVDLSFQPSQPLSKSSSSPELQTLQDILGDPGDKADVGRLSPEVKARSQSGTLDGESAAWSASGEDSRGQPEGPLPSSSPRSPSGLRPRGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVIVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV 7B915C46970D7D31 if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV DIP protein P06730 original sequence version: 164 DIP protein P06730 original sequence version: 127 DIP protein P06730 original sequence version: 126 DIP protein P06730 original sequence version: 135 DIP protein P06730 original sequence version: 151 DIP protein P06730 original sequence version: 155 DIP protein P06730 original sequence version: 160 DIP protein P06730-1 original sequence version: 187 DIP protein P06730 original sequence version: 146 B869B8DE615E699D rpb1_human DNA-directed RNA polymerase II subunit RPB1 POLR2A DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit POLR2 RNA-directed RNA polymerase II subunit RPB1 protein protein human Homo sapiens Human MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPRQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKGHGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEPRYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIRIMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKIIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTGMFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPGYSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPTSPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPASPKYTPTSPSYSPSSPEYTPTSPKYSPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTSPKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN DIP protein P24928 original sequence version: 108 DIP protein P24928 original sequence version: 147 DIP protein P24928 original sequence version: 158 DIP protein P24928 original sequence version: 157 DIP protein P24928 original sequence version: 179 DIP protein P24928 original sequence version: 181 DIP protein P24928 original sequence version: 161 DIP protein P24928 original sequence version: 175 DIP protein P24928-1 original sequence version: 175 DIP protein P24928 original sequence version: 68 DIP protein P24928 original sequence version: 116 28D6FD25693A6472 mtor-sgk1-2 1 14 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 5A, Supp 6A tsc2-sgk1-1 1 14 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 5A-2, supp 6A tsc2-sgk1-2 5 14 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept c_terminus eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role fluorescence donor fluorescence donor c_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 5B sgk1-tsc2-1 6 14 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association 5C tsc2-sgk1-3 1 14 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 42 45 physical association physical association 5D 3ayr-1 3 16 unspecified role unspecified role prey prey human-hcc-1954 HCC-1954 Ductal Carcinoma cell line hcc-1954 HCC-1954 Human Breast (Ductal) Carcinoma cell line ATCC Number = CRL-2338 HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases. ER-, PR-, ERBB2+. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology 12 unspecified role unspecified role prey prey human-hcc-1954 HCC-1954 Ductal Carcinoma cell line hcc-1954 HCC-1954 Human Breast (Ductal) Carcinoma cell line ATCC Number = CRL-2338 HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases. ER-, PR-, ERBB2+. http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-2338&Template=cellBiology 10 unspecified role unspecified role bait bait association association Supp 2C/K, Supp 5L In PDK1 knockdown cells, inhibition of PI3KA by BYL719 induced an increased binding of 4EBP1;a reduction in binding of the eukaryotic initiation factors (eIF) eIF4G and eIF4A and no change in binding of eIF4E. A similar effect is seen with PI3KA and SGK1 inhibition (Supp fig 5L). eIF4G and eIF4A and have not been added as interactors as the exact identity of these proteins is unclear. GSK2334470 (PDK1 inhibitor) appeared to decrease levels of 4EBP1 only. foxo3-tnfsf10-1 4 8 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. 3E BYL719 (CHEMBL2396661) and GSK2334470 (CHEMBL1765740) in combination.(a PI3KA inhibitor and PDK1 inhibitor, respectively) CHEMBL2396661 and CHEMBL1765740 synergistically increase the binding of O43524 to ENSG00000121858 CHEMBL2396661 and CHEMBL1765740 synergistically increases expression at RNA level of ENSG00000121858 foxo3-irs2-2 4 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. 3E-2 BYL719 (CHEMBL2396661) and GSK2334470 (CHEMBL1765740) in combination.(a PI3KA inhibitor and PDK1 inhibitor, respectively) CHEMBL2396661 and CHEMBL1765740 synergistically increase the binding of O43524 to ENSG00000185950 CHEMBL2396661 and CHEMBL1765740 synergistically increases expression at RNA level of ENSG00000185950 foxo3-tnfsf10-3 2 8 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 2H BYL719 (CHEMBL2396661) and GSK2334470 (CHEMBL1765740) in combination.(a PI3KA inhibitor and PDK1 inhibitor, respectively) CHEMBL1765740 and CHEMBL2396661 synergistically increased the binding of O43524 to ENSG00000121858 CHEMBL2396661 and CHEMBL1765740 synergistically increases expression at RNA level of ENSG00000121858 foxo3-irs2-1 2 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 2H BYL719 (CHEMBL2396661) and GSK2334470 (CHEMBL1765740) in combination.(a PI3KA inhibitor and PDK1 inhibitor, respectively) CHEMBL1765740 and CHEMBL2396661 synergistically increased the binding of O43524 to ENSG00000185950 CHEMBL2396661 and CHEMBL1765740 synergistically increases expression at RNA level of ENSG00000185950 5rwers-1 4 13 unspecified role unspecified role prey prey promoter_region_with_three CpG islands sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined possible_phospho-serines in c_terminal_domain opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 17 unspecified role unspecified role bait bait association association Supp 4H polr2a-sgk1-1 2 13 unspecified role unspecified role prey prey promoter_region_with_three CpG islands sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined possible_phospho-serines in c_terminal_domain opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 17 unspecified role unspecified role bait bait association association Supp 4Hi