IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Cancer cell 2015 imex curation Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer ronai@sbmri.org Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer V5 tagged plasmids were found on the DNASU Plasmid Repository website, The Biodesign Institute/Arizona State University. ronai@sbmri.org Accepted 2015-JUN-22 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation done; I have added BNPS-Skatole - and left both roles as neutral/unspecified.; I have added BNPS-Skatole - and left both roles as neutral/unspecified. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Cancer cell 2015 imex curation Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer ronai@sbmri.org Only protein-protein interactions imex curation human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer V5 tagged plasmids were found on the DNASU Plasmid Repository website, The Biodesign Institute/Arizona State University. ronai@sbmri.org Accepted 2015-JUN-22 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Cancer cell 2015 imex curation Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer ronai@sbmri.org Only protein-protein interactions imex curation in vitro In vitro cleavage cleavage assay cleavage weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer Purified, radiolabelled SLC1A5-V5 underwent proteolytic cleavage by BNPS-Skatole. ronai@sbmri.org Accepted 2015-JUN-22 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation done; I have added BNPS-Skatole - and left both roles as neutral/unspecified.; I have added BNPS-Skatole - and left both roles as neutral/unspecified. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Cancer cell 2015 imex curation Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer ronai@sbmri.org Only protein-protein interactions imex curation human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer ronai@sbmri.org Accepted 2015-JUN-22 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation done; I have added BNPS-Skatole - and left both roles as neutral/unspecified.; I have added BNPS-Skatole - and left both roles as neutral/unspecified. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies. Cancer cell 2015 imex curation Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer ronai@sbmri.org Only protein-protein interactions imex curation in vitro In vitro cleavage cleavage assay cleavage anti tag western anti tag western blot anti tag western Jeon YJ., Khelifa S., Ratnikov B., Scott DA., Feng Y., Parisi F., Ruller C., Lau E., Kim H., Brill LM., Jiang T., Rimm DL., Cardiff RD., Mills GB., Smith JW., Osterman AL., Kluger Y., Ronai ZA. Cancer - Interactions investigated in the context of cancer Purified, radiolabelled SLC1A5-V5 underwent proteolytic cleavage by BNPS-Skatole. ronai@sbmri.org Accepted 2015-JUN-22 by ORCHARD Cancer cell 2015 Only protein-protein interactions imex curation imex curation bnps-skatole BNPS-Skatole small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) s38a2_human Sodium-coupled neutral amino acid transporter 2 ATA2 KIAA1382 SAT2 SNAT2 Amino acid transporter A2 System A amino acid transporter 2 System N amino acid transporter 2 System A transporter 1 Solute carrier family 38 member 2 Protein 40-9-1 SLC38A2 protein protein human Homo sapiens Human MKKAEMGRFSISPDEDSSSYSSNSDFNYSYPTKQAALKSHYADVDPENQNFLLESNLGKKKYETEFHPGTTSFGMSVFNLSNAIVGSGILGLSYAMANTGIALFIILLTFVSIFSLYSVHLLLKTANEGGSLLYEQLGYKAFGLVGKLAASGSITMQNIGAMSSYLFIVKYELPLVIQALTNIEDKTGLWYLNGNYLVLLVSLVVILPLSLFRNLGYLGYTSGLSLLCMVFFLIVVICKKFQVPCPVEAALIINETINTTLTQPTALVPALSHNVTENDSCRPHYFIFNSQTVYAVPILIFSFVCHPAVLPIYEELKDRSRRRMMNVSKISFFAMFLMYLLAALFGYLTFYEHVESELLHTYSSILGTDILLLIVRLAVLMAVTLTVPVVIFPIRSSVTHLLCASKDFSWWRHSLITVSILAFTNLLVIFVPTIRDIFGFIGASAASMLIFILPSAFYIKLVKKEPMKSVQKIGALFFLLSGVLVMTGSMALIVLDWVHNAPGGGH 125C70875793D031 aaat_human Neutral amino acid transporter B(0) ASCT2 M7V1 RDR RDRC SLC1A5 Sodium-dependent neutral amino acid transporter type 2 RD114/simian type D retrovirus receptor Baboon M7 virus receptor Solute carrier family 1 member 5 protein protein human Homo sapiens Human MVADPPRDSKGLAAAEPTANGGLALASIEDQGAAAGGYCGSRDQVRRCLRANLLVLLTVVAVVAGVALGLGVSGAGGALALGPERLSAFVFPGELLLRLLRMIILPLVVCSLIGGAASLDPGALGRLGAWALLFFLVTTLLASALGVGLALALQPGAASAAINASVGAAGSAENAPSKEVLDSFLDLARNIFPSNLVSAAFRSYSTTYEERNITGTRVKVPVGQEVEGMNILGLVVFAIVFGVALRKLGPEGELLIRFFNSFNEATMVLVSWIMWYAPVGIMFLVAGKIVEMEDVGLLFARLGKYILCCLLGHAIHGLLVLPLIYFLFTRKNPYRFLWGIVTPLATAFGTSSSSATLPLMMKCVEENNGVAKHISRFILPIGATVNMDGAALFQCVAAVFIAQLSQQSLDFVKIITILVTATASSVGAAGIPAGGVLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPVPTEEGNPLLKHYRGPAGDATVASEKESVM AD61C789CCFFE934 rnf5_human E3 ubiquitin-protein ligase RNF5 RNF5 G16 RING finger protein 5 Protein G16 NG2 RMA1 Ram1 homolog RING-type E3 ubiquitin transferase RNF5 protein protein human Homo sapiens Human MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFNAHEPFRRGTGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI DIP protein Q99942 original sequence version: 56 DIP protein Q99942 original sequence version: 91 E5AFA4DE6DE85942 rnf5-slc1a5-1 4 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range range range certain certain sequence position certain association association no figure rnf5-slc1a5-2 1 8 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range range range certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1A Binding was much weaker to the Ring-domain mutant than wild-type RNF5. rnf5-slc38a2-1 1 7 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range range range certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, Supp 1B rnf5-slc1a5-3 2 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 2D/E BFA -brefeldin A (fungal antibiotic that inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus inducing ER stress) Paclitaxel; a taxane, a microtubule stabilizer, halting mitosis. rnf5-slc38a2-2 2 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 2E Paclitaxel; a taxane, a microtubule stabilizer, halting mitosis. slc1a5-rnf5-1 2 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 2D-2 BFA -brefeldin A (fungal antibiotic that inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus inducing ER stress) slc1a5-1 3 8 unspecified role unspecified role neutral component neutral component region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 4G Paclitaxel; a taxane, a microtubule stabilizer, halting mitosis. BNPS-Skatole is a peptide cleavage reagent specific for the carboxyl side of tryptophan residues. slc1a5-2 5 8 unspecified role unspecified role neutral component neutral component region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 4G Paclitaxel; a taxane, a microtubule stabilizer, halting mitosis.