IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-kg1 Human Caucasian bone marrow myelogenous leukaemia kg1 Human Caucasian bone marrow myelogenous leukaemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hl_60 Homo sapiens acute myeloid leukemia cell line hl_60 human acute myeloid leukemia cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Range of exon 5 in WT1 construct was not provided in the article hence this information has not been captured for Supp. fig 2 interaction yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hl_60 Homo sapiens acute myeloid leukemia cell line hl_60 human acute myeloid leukemia cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. WT1 recruits TET2 to regulate its target gene expression and suppress leukemia cell proliferation. Molecular cell 2015 imex curation Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer Rare diseases - Interactions investigated in the context of Rare genetic disease yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-bone-marrow Mouse bone marrow bone_marrow bone marrow anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Xiao M., Chen X., Chen L., Xu Y., Lv L., Wang P., Yang H., Ma S., Lin H., Jiao B., Ren R., Ye D., Guan KL., Xiong Y. Cancer - Interactions investigated in the context of cancer yedan@fudan.edu.cn,kuguan@ucsd.edu,yxiong@email.unc.edu Accepted 2019-DEC-05 AT 12:30 GMT AT 12:30 GMT by PPORRAS Molecular cell 2015 Only protein-protein interactions imex curation imex curation tet2_mouse Methylcytosine dioxygenase TET2 Tet2 Kiaa1546 Protein Ayu17-449 protein protein mouse Mus musculus Mouse MEQDRTTHAEGTRLSPFLIAPPSPISHTEPLAVKLQNGSPLAERPHPEVNGDTKWQSSQSCYGISHMKGSQSSHESPHEDRGYSRCLQNGGIKRTVSEPSLSGLHPNKILKLDQKAKGESNIFEESQERNHGKSSRQPNVSGLSDNGEPVTSTTQESSGADAFPTRNYNGVEIQVLNEQEGEKGRSVTLLKNKIVLMPNGATVSAHSEENTRGELLEKTQCYPDCVSIAVQSTASHVNTPSSQAAIELSHEIPQPSLTSAQINFSQTSSLQLPPEPAAMVTKACDADNASKPAIVPGTCPFQKAEHQQKSALDIGPSRAENKTIQGSMELFAEEYYPSSDRNLQASHGSSEQYSKQKETNGAYFRQSSKFPKDSISPTTVTPPSQSLLAPRLVLQPPLEGKGALNDVALEEHHDYPNRSNRTLLREGKIDHQPKTSSSQSLNPSVHTPNPPLMLPEQHQNDCGSPSPEKSRKMSEYLMYYLPNHGHSGGLQEHSQYLMGHREQEIPKDANGKQTQGSVQAAPGWIELKAPNLHEALHQTKRKDISLHSVLHSQTGPVNQMSSKQSTGNVNMPGGFQRLPYLQKTAQPEQKAQMYQVQVNQGPSPGMGDQHLQFQKALYQECIPRTDPSSEAHPQAPSVPQYHFQQRVNPSSDKHLSQQATETQRLSGFLQHTPQTQASQTPASQNSNFPQICQQQQQQQLQRKNKEQMPQTFSHLQGSNDKQREGSCFGQIKVEESFCVGNQYSKSSNFQTHNNTQGGLEQVQNINKNFPYSKILTPNSSNLQILPSNDTHPACEREQALHPVGSKTSNLQNMQYFPNNVTPNQDVHRCFQEQAQKPQQASSLQGLKDRSQGESPAPPAEAAQQRYLVHNEAKALPVPEQGGSQTQTPPQKDTQKHAALRWLLLQKQEQQQTQQSQPGHNQMLRPIKTEPVSKPSSYRYPLSPPQENMSSRIKQEISSPSRDNGQPKSIIETMEQHLKQFQLKSLCDYKALTLKSQKHVKVPTDIQAAESENHARAAEPQATKSTDCSVLDDVSESDTPGEQSQNGKCEGCNPDKDEAPYYTHLGAGPDVAAIRTLMEERYGEKGKAIRIEKVIYTGKEGKSSQGCPIAKWVYRRSSEEEKLLCLVRVRPNHTCETAVMVIAIMLWDGIPKLLASELYSELTDILGKCGICTNRRCSQNETRNCCCQGENPETCGASFSFGCSWSMYYNGCKFARSKKPRKFRLHGAEPKEEERLGSHLQNLATVIAPIYKKLAPDAYNNQVEFEHQAPDCCLGLKEGRPFSGVTACLDFSAHSHRDQQNMPNGSTVVVTLNREDNREVGAKPEDEQFHVLPMYIIAPEDEFGSTEGQEKKIRMGSIEVLQSFRRRRVIRIGELPKSCKKKAEPKKAKTKKAARKRSSLENCSSRTEKGKSSSHTKLMENASHMKQMTAQPQLSGPVIRQPPTLQRHLQQGQRPQQPQPPQPQPQTTPQPQPQPQHIMPGNSQSVGSHCSGSTSVYTRQPTPHSPYPSSAHTSDIYGDTNHVNFYPTSSHASGSYLNPSNYMNPYLGLLNQNNQYAPFPYNGSVPVDNGSPFLGSYSPQAQSRDLHRYPNQDHLTNQNLPPIHTLHQQTFGDSPSKYLSYGNQNMQRDAFTTNSTLKPNVHHLATFSPYPTPKMDSHFMGAASRSPYSHPHTDYKTSEHHLPSHTIYSYTAAASGSSSSHAFHNKENDNIANGLSRVLPGFNHDRTASAQELLYSLTGSSQEKQPEVSGQDAAAVQEIEYWSDSEHNFQDPCIGGVAIAPTHGSILIECAKCEVHATTKVNDPDRNHPTRISLVLYRHKNLFLPKHCLALWEAKMAEKARKEEECGKNGSDHVSQKNHGKQEKREPTGPQEPSYLRFIQSLAENTGSVTTDSTVTTSPYAFTQVTGPYNTFV F49DEE206CD05670 wt1_human Wilms tumor protein WT33 WT1 protein protein human Homo sapiens Human MGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL 11C7FA3D485096B2 tet2_human Methylcytosine dioxygenase TET2 TET2 KIAA1546 Nbla00191 protein protein human Homo sapiens Human MEQDRTNHVEGNRLSPFLIPSPPICQTEPLATKLQNGSPLPERAHPEVNGDTKWHSFKSYYGIPCMKGSQNSRVSPDFTQESRGYSKCLQNGGIKRTVSEPSLSGLLQIKKLKQDQKANGERRNFGVSQERNPGESSQPNVSDLSDKKESVSSVAQENAVKDFTSFSTHNCSGPENPELQILNEQEGKSANYHDKNIVLLKNKAVLMPNGATVSASSVEHTHGELLEKTLSQYYPDCVSIAVQKTTSHINAINSQATNELSCEITHPSHTSGQINSAQTSNSELPPKPAAVVSEACDADDADNASKLAAMLNTCSFQKPEQLQQQKSVFEICPSPAENNIQGTTKLASGEEFCSGSSSNLQAPGGSSERYLKQNEMNGAYFKQSSVFTKDSFSATTTPPPPSQLLLSPPPPLPQVPQLPSEGKSTLNGGVLEEHHHYPNQSNTTLLREVKIEGKPEAPPSQSPNPSTHVCSPSPMLSERPQNNCVNRNDIQTAGTMTVPLCSEKTRPMSEHLKHNPPIFGSSGELQDNCQQLMRNKEQEILKGRDKEQTRDLVPPTQHYLKPGWIELKAPRFHQAESHLKRNEASLPSILQYQPNLSNQMTSKQYTGNSNMPGGLPRQAYTQKTTQLEHKSQMYQVEMNQGQSQGTVDQHLQFQKPSHQVHFSKTDHLPKAHVQSLCGTRFHFQQRADSQTEKLMSPVLKQHLNQQASETEPFSNSHLLQHKPHKQAAQTQPSQSSHLPQNQQQQQKLQIKNKEEILQTFPHPQSNNDQQREGSFFGQTKVEECFHGENQYSKSSEFETHNVQMGLEEVQNINRRNSPYSQTMKSSACKIQVSCSNNTHLVSENKEQTTHPELFAGNKTQNLHHMQYFPNNVIPKQDLLHRCFQEQEQKSQQASVLQGYKNRNQDMSGQQAAQLAQQRYLIHNHANVFPVPDQGGSHTQTPPQKDTQKHAALRWHLLQKQEQQQTQQPQTESCHSQMHRPIKVEPGCKPHACMHTAPPENKTWKKVTKQENPPASCDNVQQKSIIETMEQHLKQFHAKSLFDHKALTLKSQKQVKVEMSGPVTVLTRQTTAAELDSHTPALEQQTTSSEKTPTKRTAASVLNNFIESPSKLLDTPIKNLLDTPVKTQYDFPSCRCVEQIIEKDEGPFYTHLGAGPNVAAIREIMEERFGQKGKAIRIERVIYTGKEGKSSQGCPIAKWVVRRSSSEEKLLCLVRERAGHTCEAAVIVILILVWEGIPLSLADKLYSELTETLRKYGTLTNRRCALNEERTCACQGLDPETCGASFSFGCSWSMYYNGCKFARSKIPRKFKLLGDDPKEEEKLESHLQNLSTLMAPTYKKLAPDAYNNQIEYEHRAPECRLGLKEGRPFSGVTACLDFCAHAHRDLHNMQNGSTLVCTLTREDNREFGGKPEDEQLHVLPLYKVSDVDEFGSVEAQEEKKRSGAIQVLSSFRRKVRMLAEPVKTCRQRKLEAKKAAAEKLSSLENSSNKNEKEKSAPSRTKQTENASQAKQLAELLRLSGPVMQQSQQPQPLQKQPPQPQQQQRPQQQQPHHPQTESVNSYSASGSTNPYMRRPNPVSPYPNSSHTSDIYGSTSPMNFYSTSSQAAGSYLNSSNPMNPYPGLLNQNTQYPSYQCNGNLSVDNCSPYLGSYSPQSQPMDLYRYPSQDPLSKLSLPPIHTLYQPRFGNSQSFTSKYLGYGNQNMQGDGFSSCTIRPNVHHVGKLPPYPTHEMDGHFMGATSRLPPNLSNPNMDYKNGEHHSPSHIIHNYSAAPGMFNSSLHALHLQNKENDMLSHTANGLSKMLPALNHDRTACVQGGLHKLSDANGQEKQPLALVQGVASGAEDNDEVWSDSEQSFLDPDIGGVAVAPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNEPKHGLALWEAKMAEKAREKEEECEKYGPDYVPQKSHGKKVKREPAEPHETSEPTYLRFIKSLAERTMSVTTDSTVTTSPYAFTRVTGPYNRYI 6D740305EE2A8D46 wt1_mouse Wilms tumor protein homolog Wt1 Wt-1 protein protein mouse Mus musculus Mouse MGSDVRDLNALLPAVSSLGGGGGGCGLPVSGARQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTLHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPTIRNQGYSTVTFDGAPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGMAAGSSSSVKWTEGQSNHGIGYESENHTAPILCGAQYRIHTHGVFRGIQDVRRVSGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWHSCQKKFARSDELVRHHNMHQRNMTKLHVAL Potential role in transcriptional regulation. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3' mint 962381E9C8D7A380 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 tet2-wt1-1 8 12 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 2A wt1-tet2-1 2 12 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 11 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 2A tet2-wt1-2 5 13 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 2B wt1-tet2-2 9 13 unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 10 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Figure 2B tet2-wt1-3 6 11 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 12 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2c tp53-wt1-1 6 11 unspecified role unspecified role prey prey living cell living cell 14 anti tag western anti tag western blot anti tag western western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2c tet2-wt1-4 1 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 2d tet2-wt1-5 1 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 2e tet2-wt1-6 3 11 weight by staining molecular weight estimation by staining weight by staining western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 12 weight by staining molecular weight estimation by staining weight by staining western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Figure 2f, 2g tet2-wt1-7 4 11 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q6N021:p.Asp1242Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D V Tue Jun 30 19:30:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1242Val' catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q6N021:p.Arg1302Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 18:21:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1302Gly' Q6N021:p.Leu1340Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L R Tue Jun 30 17:37:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1340Arg' Q6N021:p.Phe1368Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F L Tue Jun 30 18:06:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe1368Leu' Q6N021:p.Gly1256Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 17:24:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1256Asp' Q6N021:p.Asp1314Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D G Tue Jun 30 19:50:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1314Gly' Q6N021:p.Thr1884Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 17:05:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1884Ala' Q6N021:p.Gly1861Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G R Tue Jun 30 15:37:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1861Arg' Q6N021:p.Ala1505Thr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A T Tue Jun 30 16:42:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1505Thr' Q6N021:p.Pro1617His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P H Tue Jun 30 17:13:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro1617His' physical association physical association Figure 5a tet2-wt1-8 7 11 unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level exon 5 binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined KTS region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure S2c, S2b, S2d tet2-wt1-9 1 12 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q6N021:p.Asp1242Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D V Tue Jun 30 20:24:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1242Val' catalytic domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q6N021:p.Arg1302Gly mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R G Tue Jun 30 18:45:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1302Gly' Q6N021:p.Leu1340Arg mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain L R Tue Jun 30 19:38:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu1340Arg' Q6N021:p.Phe1368Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F L Tue Jun 30 18:28:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe1368Leu' Q6N021:p.Gly1256Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G D Tue Jun 30 20:13:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1256Asp' Q6N021:p.Asp1314Gly mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D G Tue Jun 30 15:39:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1314Gly' Q6N021:p.Thr1884Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 16:23:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1884Ala' Q6N021:p.Gly1861Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G R Tue Jun 30 17:04:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1861Arg' Q6N021:p.Ala1505Thr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A T Tue Jun 30 15:49:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala1505Thr' Q6N021:p.Pro1617His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain P H Tue Jun 30 16:10:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro1617His' 11 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure S5a