IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. Cell (0092-8674) 2014 imex curation Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Only protein-protein interactions in vitro In vitro peptide array peptide array antibody detection identification by antibody antibody detection Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Accepted 2015-MAR-03 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. Cell (0092-8674) 2014 imex curation Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Only protein-protein interactions human-calu_1 human calu_1 calu_1 Human lung carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer no gavin_macbeath@harvard.edu, marc@hms.harvard.edu Accepted 2015-MAR-03 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. Cell (0092-8674) 2014 imex curation Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Accepted 2015-MAR-03 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. Cell (0092-8674) 2014 imex curation Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Only protein-protein interactions human-nci_h661 Human lung large cell carcinoma cell line NCIH661 nci_h661 Human lung large cell carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer no gavin_macbeath@harvard.edu, marc@hms.harvard.edu Accepted 2015-MAR-03 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. Cell (0092-8674) 2014 imex curation Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Only protein-protein interactions human-focus hcc Human-Focus hepatocellular carcinoma cell line focus_hcc Human FOCUS hepatocellular carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer no gavin_macbeath@harvard.edu, marc@hms.harvard.edu Accepted 2015-MAR-03 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. A noncanonical Frizzled2 pathway regulates epithelial-mesenchymal transition and metastasis. Cell (0092-8674) 2014 imex curation Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer gavin_macbeath@harvard.edu, marc@hms.harvard.edu Only protein-protein interactions human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gujral T.S., Chan M., Peshkin L., Sorger P.K., Kirschner M.W., MacBeath G. Cancer - Interactions investigated in the context of cancer no gavin_macbeath@harvard.edu, marc@hms.harvard.edu Accepted 2015-MAR-03 by MGT Cell (0092-8674) 2014 Only protein-protein interactions imex curation stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D DIP protein P40763 original sequence version: 157 DIP protein P40763-1 original sequence version: 147 DIP protein P40763-1 original sequence version: 135 DIP protein P40763-1 original sequence version: 157 DIP protein P40763 original sequence version: 165 DIP protein P40763 original sequence version: 182 DIP protein P40763 original sequence version: 135 6C00632211C8012D fyn_mouse Tyrosine-protein kinase Fyn p59-Fyn Fyn Proto-oncogene c-Fyn protein protein mouse Mus musculus Mouse MGCVQCKDKEAAKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGENL 99558702596DAEE0 fzd2_human Frizzled-2 FZD2 FzE2 protein protein human Homo sapiens Human MRPRSALPRLLLPLLLLPAAGPAQFHGEKGISIPDHGFCQPISIPLCTDIAYNQTIMPNLLGHTNQEDAGLEVHQFYPLVKVQCSPELRFFLCSMYAPVCTVLEQAIPPCRSICERARQGCEALMNKFGFQWPERLRCEHFPRHGAEQICVGQNHSEDGAPALLTTAPPPGLQPGAGGTPGGPGGGGAPPRYATLEHPFHCPRVLKVPSYLSYKFLGERDCAAPCEPARPDGSMFFSQEETRFARLWILTWSVLCCASTFFTVTTYLVDMQRFRYPERPIIFLSGCYTMVSVAYIAGFVLQERVVCNERFSEDGYRTVVQGTKKEGCTILFMMLYFFSMASSIWWVILSLTWFLAAGMKWGHEAIEANSQYFHLAAWAVPAVKTITILAMGQIDGDLLSGVCFVGLNSLDPLRGFVLAPLFVYLFIGTSFLLAGFVSLFRIRTIMKHDGTKTEKLERLMVRIGVFSVLYTVPATIVIACYFYEQAFREHWERSWVSQHCKSLAIPCPAHYTPRMSPDFTVYMIKYLMTLIVGITSGFWIWSGKTLHSWRKFYTRLTNSRHGETTV AA387876A1DDE063 fzd2-stat3 5 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 2F stat3-fzd2 5 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig. 2F fzd2-stat3-1 6 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 3G fzd2-stat3-2 4 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 3G fzd2-stat3-3 2 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 3G fzd2-fyn 1 8 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait tyr-552 (required) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 30 28 direct interaction direct interaction Fig. 5C Q14332 increases activity of P39688 fyn-fzd2-3 3 7 unspecified role unspecified role prey prey human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line 9 unspecified role unspecified role prey prey human-bt_549 Homo sapiens BT_549 Breast ductal carcinoma cell line bt_549 BT 549 Human Breast ductal carcinoma cell line 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) association association Fig. 5D fyn-fzd2-4 3 7 unspecified role unspecified role prey prey human-calu_1 human calu_1 calu_1 Human lung carcinoma cells 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain true ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey human-calu_1 human calu_1 calu_1 Human lung carcinoma cells association association Fig. 5D fyn-fzd2-5 3 7 unspecified role unspecified role prey prey human-focus hcc Human-Focus hepatocellular carcinoma cell line focus_hcc Human FOCUS hepatocellular carcinoma cell line 9 unspecified role unspecified role prey prey human-focus hcc Human-Focus hepatocellular carcinoma cell line focus_hcc Human FOCUS hepatocellular carcinoma cell line 8 unspecified role unspecified role bait bait sh2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain true region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true ecoli Escherichia coli (strain K12) association association Fig. 5D