IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus. Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus. Cancer Cell (1535-6108) 2014 imex curation Li W., Cooper J., Zhou L., Yang C., Erdjument-Bromage H., Zagzag D., Snuderl M., Ladanyi M., Hanemann C.O., Zhou P., Karajannis M.A., Giancotti F.G. Cancer - Interactions investigated in the context of cancer The authors have stated that the origins of these and related constructs are as follows:- Flag-HA-Merlin is from mouse, Flag-MST1/2 are from human, Myc-Lats1/2 are from human, His-myc-ubiquitin is from Yeast. Tag location has been derived from Li et al -2010 - N terminal when the tag is stated before the protein, and C terminal when after. F-Giancotti@ski.mskcc.org,Liw@mskcc.org 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down tag visualisation tag visualisation Li W., Cooper J., Zhou L., Yang C., Erdjument-Bromage H., Zagzag D., Snuderl M., Ladanyi M., Hanemann C.O., Zhou P., Karajannis M.A., Giancotti F.G. Cancer - Interactions investigated in the context of cancer The authors have stated that the origins of these and related constructs are as follows:- Flag-HA-Merlin is from mouse, Flag-MST1/2 are from human, Myc-Lats1/2 are from human, His-myc-ubiquitin is from Yeast. Tag location has been derived from Li et al -2010 - N terminal when the tag is stated before the protein, and C terminal when after. liw@mskcc.org,f-giancotti@ski.mskcc.org F-Giancotti@ski.mskcc.org,Liw@mskcc.org Accepted 2014-AUG-26 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus. Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus. Cancer Cell (1535-6108) 2014 imex curation Li W., Cooper J., Zhou L., Yang C., Erdjument-Bromage H., Zagzag D., Snuderl M., Ladanyi M., Hanemann C.O., Zhou P., Karajannis M.A., Giancotti F.G. Cancer - Interactions investigated in the context of cancer The authors have stated that the origins of these and related constructs are as follows:- Flag-HA-Merlin is from mouse, Flag-MST1/2 are from human, Myc-Lats1/2 are from human, His-myc-ubiquitin is from Yeast. Tag location has been derived from Li et al -2010 - N terminal when the tag is stated before the protein, and C terminal when after. F-Giancotti@ski.mskcc.org,Liw@mskcc.org 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li W., Cooper J., Zhou L., Yang C., Erdjument-Bromage H., Zagzag D., Snuderl M., Ladanyi M., Hanemann C.O., Zhou P., Karajannis M.A., Giancotti F.G. Cancer - Interactions investigated in the context of cancer Figs 5E and Supp 5H appear to have used exactly the same constructs - but the images are fractionally different, so not a duplicated image - not sure if I have missed something here... The authors have stated that the origins of these and related constructs are as follows:- Flag-HA-Merlin is from mouse, Flag-MST1/2 are from human, Myc-Lats1/2 are from human, His-myc-ubiquitin is from Yeast. Tag location has been derived from Li et al -2010 - N terminal when the tag is stated before the protein, and C terminal when after. liw@mskcc.org,f-giancotti@ski.mskcc.org F-Giancotti@ski.mskcc.org,Liw@mskcc.org Accepted 2014-AUG-26 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus. Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus. Cancer Cell (1535-6108) 2014 imex curation Li W., Cooper J., Zhou L., Yang C., Erdjument-Bromage H., Zagzag D., Snuderl M., Ladanyi M., Hanemann C.O., Zhou P., Karajannis M.A., Giancotti F.G. Cancer - Interactions investigated in the context of cancer The authors have stated that the origins of these and related constructs are as follows:- Flag-HA-Merlin is from mouse, Flag-MST1/2 are from human, Myc-Lats1/2 are from human, His-myc-ubiquitin is from Yeast. Tag location has been derived from Li et al -2010 - N terminal when the tag is stated before the protein, and C terminal when after. F-Giancotti@ski.mskcc.org,Liw@mskcc.org 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro ubiquitinase assay ubiquitinase assay ubiquitinase assay western blot western blot Immuno blot Immuno blot Li W., Cooper J., Zhou L., Yang C., Erdjument-Bromage H., Zagzag D., Snuderl M., Ladanyi M., Hanemann C.O., Zhou P., Karajannis M.A., Giancotti F.G. Cancer - Interactions investigated in the context of cancer The authors have stated that the origins of these and related constructs are as follows:- Flag-HA-Merlin is from mouse, Flag-MST1/2 are from human, Myc-Lats1/2 are from human, His-myc-ubiquitin is from Yeast. Tag location has been derived from Li et al -2010 - N terminal when the tag is stated before the protein, and C terminal when after. liw@mskcc.org,f-giancotti@ski.mskcc.org F-Giancotti@ski.mskcc.org,Liw@mskcc.org Accepted 2014-AUG-26 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus. Merlin/NF2 Loss-Driven Tumorigenesis Linked to CRL4(DCAF1)-Mediated Inhibition of the Hippo Pathway Kinases Lats1 and 2 in the Nucleus. Cancer Cell (1535-6108) 2014 imex curation Li W., Cooper J., Zhou L., Yang C., Erdjument-Bromage H., Zagzag D., Snuderl M., Ladanyi M., Hanemann C.O., Zhou P., Karajannis M.A., Giancotti F.G. Cancer - Interactions investigated in the context of cancer The authors have stated that the origins of these and related constructs are as follows:- Flag-HA-Merlin is from mouse, Flag-MST1/2 are from human, Myc-Lats1/2 are from human, His-myc-ubiquitin is from Yeast. Tag location has been derived from Li et al -2010 - N terminal when the tag is stated before the protein, and C terminal when after. F-Giancotti@ski.mskcc.org,Liw@mskcc.org 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-meso-33 Human Meso-33, Mesothelioma cell line, Merlin deficient meso-33 Human MESO-33 mesothelioma cell line, Merlin-deficient fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Li W., Cooper J., Zhou L., Yang C., Erdjument-Bromage H., Zagzag D., Snuderl M., Ladanyi M., Hanemann C.O., Zhou P., Karajannis M.A., Giancotti F.G. Cancer - Interactions investigated in the context of cancer The authors have stated that the origins of these and related constructs are as follows:- Flag-HA-Merlin is from mouse, Flag-MST1/2 are from human, Myc-Lats1/2 are from human, His-myc-ubiquitin is from Yeast. Tag location has been derived from Li et al -2010 - N terminal when the tag is stated before the protein, and C terminal when after. liw@mskcc.org,f-giancotti@ski.mskcc.org F-Giancotti@ski.mskcc.org,Liw@mskcc.org Accepted 2014-AUG-26 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation merl_mouse Merlin Moesin-ezrin-radixin-like protein Neurofibromin-2 Schwannomin Nf2 Nf-2 protein protein mouse Mus musculus Mouse MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPPPLPPDIPSFDIIADSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDVLHSESSDRGGPSSKHNTIKKLTLQSAKSRVAFFEEL 8D06F557E3435851 dcaf1_human DDB1- and CUL4-associated factor 1 DCAF1 HIV-1 Vpr-binding protein KIAA0800 RIP Serine/threonine-protein kinase VPRBP VPRBP Vpr-interacting protein protein protein human Homo sapiens Human MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE DIP protein Q9Y4B6 original sequence version: 77 DIP protein Q9Y4B6-1 original sequence version: 99 7E71AB2CAC4962C5 lats2_human Serine/threonine-protein kinase LATS2 LATS2 KPM Kinase phosphorylated during mitosis protein Large tumor suppressor homolog 2 Serine/threonine-protein kinase kpm Warts-like kinase protein protein human Homo sapiens Human MRPKTFPATTYSGNSRQRLQEIREGLKQPSKSSVQGLPAGPNSDTSLDAKVLGSKDATRQQQQMRATPKFGPYQKALREIRYSLLPFANESGTSAAAEVNRQMLQELVNAGCDQEMAGRALKQTGSRSIEAALEYISKMGYLDPRNEQIVRVIKQTSPGKGLMPTPVTRRPSFEGTGDSFASYHQLSGTPYEGPSFGADGPTALEEMPRPYVDYLFPGVGPHGPGHQHQHPPKGYGASVEAAGAHFPLQGAHYGRPHLLVPGEPLGYGVQRSPSFQSKTPPETGGYASLPTKGQGGPPGAGLAFPPPAAGLYVPHPHHKQAGPAAHQLHVLGSRSQVFASDSPPQSLLTPSRNSLNVDLYELGSTSVQQWPAATLARRDSLQKPGLEAPPRAHVAFRPDCPVPSRTNSFNSHQPRPGPPGKAEPSLPAPNTVTAVTAAHILHPVKSVRVLRPEPQTAVGPSHPAWVPAPAPAPAPAPAPAAEGLDAKEEHALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSEQYDLDSLCAGMEQSLRAGPNEPEGGDKSRKSAKGDKGGKDKKQIQTSPVPVRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQEMAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAFGEVCLACKVDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDLWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFSSDIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPNNKHPEHAFYEFTFRRFFDDNGYPFRCPKPSGAEASQAESSDLESSDLVDQTEGCQPVYV DIP protein Q9NRM7 original sequence version: 137 DIP protein Q9NRM7 original sequence version: 134 2866F6B75637AD36 ubiq_yeast Ubiquitin UBI1 RPL40A YIL148W UBI2 RPL40B YKR094C UBI3 RPS31 YLR167W L9470.14 UBI4 SCD2 YLL039C protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG obsolete protein 9AC936269D38403E lats1_human Serine/threonine-protein kinase LATS1 Large tumor suppressor homolog 1 WARTS protein kinase LATS1 WARTS protein protein human Homo sapiens Human MKRSEKPEGYRQMRPKTFPASNYTVSSRQMLQEIRESLRNLSKPSDAAKAEHNMSKMSTEDPRQVRNPPKFGTHHKALQEIRNSLLPFANETNSSRSTSEVNPQMLQDLQAAGFDEDMVIQALQKTNNRSIEAAIEFISKMSYQDPRREQMAAAAARPINASMKPGNVQQSVNRKQSWKGSKESLVPQRHGPPLGESVAYHSESPNSQTDVGRPLSGSGISAFVQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGNMEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSSSKFNFPSGRPGMQNGTGQTDFMIHQNVVPAGTVNRQPPPPYPLTAANGQSPSALQTGGSAAPSSYTNGSIPQSMMVPNRNSHNMELYNISVPGLQTNWPQSSSAPAQSSPSSGHEIPTWQPNIPVRSNSFNNPLGNRASHSANSQPSATTVTAITPAPIQQPVKSMRVLKPELQTALAPTHPSWIPQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYPKHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKNKKDEERRESRIQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVGLSQDAQDQMRKMLCQKESNYIRLKRAKMDKSMFVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSNEWGDPSSCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEMLVGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPEASDLIIKLCRGPEDRLGKNGADEIKAHPFFKTIDFSSDLRQQSASYIPKITHPTDTSNFDPVDPDKLWSDDNEEENVNDTLNGWYKNGKHPEHAFYEFTFRRFFDDNGYPYNYPKPIEYEYINSQGSEQQSDEDDQNTGSEIKNRDLVYV DIP protein O95835 original sequence version: 133 DIP protein O95835 original sequence version: 147 DIP protein O95835 original sequence version: 144 DIP protein O95835 original sequence version: 142 DIP protein O95835-1 original sequence version: 142 11CFBCD8FD87DCD8 amot_human Angiomotin AMOT KIAA1071 protein protein human Homo sapiens Human MRNSEEQPSGGTTVLQRLLQEQLRYGNPSENRSLLAIHQQATGNGPPFPSGSGNPGPQSDVLSPQDHHQQLVAHAARQEPQGQEIQSENLIMEKQLSPRMQNNEELPTYEEAKVQSQYFRGQQHASVGAAFYVTGVTNQKMRTEGRPSVQRLNPGKMHQDEGLRDLKQGHVRSLSERLMQMSLATSGVKAHPPVTSAPLSPPQPNDLYKNPTSSSEFYKAQGPLPNQHSLKGMEHRGPPPEYPFKGMPPQSVVCKPQEPGHFYSEHRLNQPGRTEGQLMRYQHPPEYGAARPAQDISLPLSARNSQPHSPTSSLTSGGSLPLLQSPPSTRLSPARHPLVPNQGDHSAHLPRPQQHFLPNQAHQGDHYRLSQPGLSQQQQQQQQQHHHHHHHQQQQQQQPQQQPGEAYSAMPRAQPSSASYQPVPADPFAIVSRAQQMVEILSDENRNLRQELEGCYEKVARLQKVETEIQRVSEAYENLVKSSSKREALEKAMRNKLEGEIRRMHDFNRDLRERLETANKQLAEKEYEGSEDTRKTISQLFAKNKESQREKEKLEAELATARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEKREQLEHRLRTRLERELESLRIQQRQGNCQPTNVSEYNAAALMELLREKEERILALEADMTKWEQKYLEENVMRHFALDAAATVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKDAMIKVLQQRSRKEPSKTEQLSCMRPAKSLMSISNAGSGLLSHSSTLTGSPIMEEKRDDKSWKGSLGILLGGDYRAEYVPSTPSPVPPSTPLLSAHSKTGSRDCSTQTERGTESNKTAAVAPISVPAPVAAAATAAAITATAATITTTMVAAAPVAVAAAAAPAAAAAPSPATAAATAAAVSPAAAGQIPAAASVASAAAVAPSAAAAAAVQVAPAAPAPVPAPALVPVPAPAAAQASAPAQTQAPTSAPAVAPTPAPTPTPAVAQAEVPASPATGPGPHRLSIPSLTCNPDKTDGPVFHSNTLERKTPIQILGQEPDAEMVEYLI DIP protein Q4VCS5 original sequence version: 101 D7E7021E9535A628 vprbp-lats1 2 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2A, 3E transfected HA-Merlin vprbp-lats1-1 2 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2Ai vprbp-lats1-3 2 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range association association 5E, Supp 5H All nuclear-defective and patient-derived mutants of Merlin failed to associate with DCAF1, but none of them exhibited a reduced ability to interact with Lats1. nf2-lats1-4 2 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P46662:p.Val24_Val27delinsAlaAlaAlaAla mutation mutation certain certain sequence position certain certain certain sequence position certain VRIV AAAA Thu Jul 07 12:17:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val24ala,arg25ala,ile26ala,val27ala Fri Jul 22 11:15:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val24ala,arg25ala,ile26ala,val27ala' Wed May 02 14:30:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_arg_ile_val24-27ala_ala_ala_ala' Tue Jun 30 20:25:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val24_Val27delinsAlaAlaAlaAla' P46662:p.Leu46Arg mutation mutation certain certain sequence position certain certain certain sequence position certain L R Wed May 02 15:20:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu46arg' Tue Jun 30 17:10:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu46Arg' P46662:p.Leu64Pro mutation mutation certain certain sequence position certain certain certain sequence position certain L P Wed May 02 20:30:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu64pro' Tue Jun 30 17:53:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu64Pro' P46662:p.Leu141Pro mutation mutation certain certain sequence position certain certain certain sequence position certain L P Wed May 02 19:32:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu141pro' Tue Jun 30 17:15:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu141Pro' P46662:p.Ala211Asp mutation mutation certain certain sequence position certain certain certain sequence position certain A D Wed May 02 20:23:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala211asp' Tue Jun 30 18:16:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala211Asp' P46662:p.Glu270Gly mutation mutation certain certain sequence position certain certain certain sequence position certain E G Wed May 02 18:19:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu270gly' Tue Jun 30 17:25:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu270Gly' physical association physical association 5Eii,Supp 5H All nuclear-defective and patient-derived mutants of Merlin failed to associate with DCAF1, but none of them exhibited a reduced ability to interact with Lats1. lats1-vprbp 2 6 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region_including_kinase_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp 2B lats1-ubi1-1 3 6 enzyme enzyme unspecified role unspecified role n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role ancillary ancillary region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 enzyme target enzyme target substrate substrate unspecified role unspecified role n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. ubiquitination ubiquitination reaction ubiquitination 3B The recombinant proteins, E1, E2 (UBCH5c), GST-Cul4A, His-Rbx1 and GST-DDB1 were also present, -origins unknown. lats1-vprbp-1 1 9 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range kinase_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 58 61 physical association physical association Supp 2C lats1-ubi1-2 3 6 enzyme enzyme unspecified role unspecified role n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role ancillary ancillary region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 enzyme target enzyme target substrate substrate unspecified role unspecified role n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. ubiquitination ubiquitination reaction ubiquitination Supp 3F The recombinant proteins, E1, E2 (UBCH5c), GST-Cul4A, His-Rbx1 and GST-DDB1 were also present, -origins unknown. nf2-amot-1 2 10 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role prey prey association association Supp fig 4F Nuclear-defective mutants of Merlin failed to associate with DCAF1, but they did not exhibit a reduced ability to interact with Angiomotin. nf2-amot-1 2 5 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P46662:p.Val24_Val27delinsAlaAlaAlaAla mutation mutation certain certain sequence position certain certain certain sequence position certain VRIV AAAA Thu Jul 07 12:17:45 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val24ala,arg25ala,ile26ala,val27ala Fri Jul 22 10:53:18 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val24ala,arg25ala,ile26ala,val27ala' Wed May 02 18:20:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_arg_ile_val24-27ala_ala_ala_ala' Tue Jun 30 16:07:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val24_Val27delinsAlaAlaAlaAla' 10 unspecified role unspecified role prey prey physical association physical association Supp fig 4Fi Nuclear-defective mutants of Merlin failed to associate with DCAF1, but they did not exhibit a reduced ability to interact with Angiomotin. lats1-nf2-2 4 9 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supp 5E nf2-lats1-5 2 9 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range coiled_coil_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supp 5F All nuclear-defective and patient-derived mutants of Merlin failed to associate with DCAF1, but none of them exhibited a reduced ability to interact with Lats1. lats1-nf2-3 2 9 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range kinase_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp 5G All nuclear-defective and patient-derived mutants of Merlin failed to associate with DCAF1, but none of them exhibited a reduced ability to interact with Lats1.