IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-molm-14 Human MOLM-14 AML cell line with FLT3 ITD molm-14 MOLM-14 AML cell line with FLT3 ITD anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-eol-1 Human eosinophilic leukemia cell line eol-1 EOL-1 Human eosinophilic leukaemia, lymphoblastoid cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-mv4-11 Human MV4-11, Acute Monocytic Leukemia cell line mv4-11 MV4-11 a human MLL leukemia cell line https://www.lgcstandards-atcc.org/Products/All/CRL-9591.aspx Human acute monocytic leukemia cell line. Described to carry a 4;11 translocation; MV4-11 carries an FLT3 internal tandem duplication. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293e Human HEK293E or 293 c18 cell line 293e HEK293E or 293 c18 http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-10852&Template=cellBiology This line is a derivative of 293 (see ATCCCRL-1573) the cells express EBNA1. (Epstein Barr nuclear antigen) anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. Figure 3G - the authors have not supplied any info regarding the exact sequences/amino acid numbers for the Delta-C terminal SH2 domain, nor the Delta-NLS construct. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293e Human HEK293E or 293 c18 cell line 293e HEK293E or 293 c18 http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=CRL-10852&Template=cellBiology This line is a derivative of 293 (see ATCCCRL-1573) the cells express EBNA1. (Epstein Barr nuclear antigen) anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. Pablo and I thought the best way to show the insertion mutation (FLT3-ITD) was as a "region" - possibly it could be a Fusion Protein? The exact sequences for FLT3-ITD constructs are not given in this publication. Thus as a representative example of the ITD (Internal Tandem Repeat) Mutation, the FLT3 W/N51 mutation has been used, as taken from: pmid: 23103841 and 14981546. (James Griffin, donator or these constructs, was contributing author to 14981546.) kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay sequence tag sequence tag identification sequence tag Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer The ratio of the exponential values corresponding to the panel of tyrosine phosphorylated with FLT3 + SYK and those corresponding to the same panel of tyrosine phosphorylated with FLT3 only, was ultimately log2-transformed and projected into a heatmap. Results from the heatmap do not distinguish between FLT3 phosphorylation or possible FLT3 auto-phosphorylation. Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-nb4 Human NB4 APL cell line nb4 NB4 human acute promyelocytic leukemia cells Human acute promyelocytic leukemia established from the bone marrow of a 23- year-old woman with acute promyelocytic leukemia (APL = AML FAB M3) in second relapse in 1989; patented cell line; cells carry the t(15;17) PML-RARA fusion gene confirmed as human with IEF of AST, MDH anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-molm-13 Human MOLM-13 AML cell line with ITD of FLT3 molm-13 MOLM-13 AML cell line with ITD of FLT3 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-kbm-3 KBM-3 acute myelomonocytic leukemia cell line with FLT3 ITD kbm-3 KBM-3 acute myelomonocytic leukemia cell line with FLT3 ITD anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-kg1 Human Caucasian bone marrow myelogenous leukaemia kg1 Human Caucasian bone marrow myelogenous leukaemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-nomo-1 human nomo-1 nomo-1 NOMO-1 Human AML cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-aml Human AML cells aml Acute myeloid leukemia cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-thp-1 Homo sapiens acute monocytic leukemia cells thp-1 Human acute monocytic leukemia cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. SYK Is a Critical Regulator of FLT3 in Acute Myeloid Leukemia. Cancer Cell (1535-6108) 2014 imex curation Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-skm-1 Human SKM-1 AML cell line skm-1 Human SKM-1 AML cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Puissant A., Fenouille N., Alexe G., Pikman Y., Bassil C.F., Mehta S., Du J., Kazi J.U., Luciano F., Rönnstrand L., Kung A.L., Aster J.C., Galinsky I., Stone R.M., Deangelo D.J., Hemann M.T., Stegmaier K. Cancer - Interactions investigated in the context of cancer Results from Kinase assays carried out using IPs from cell extracts have not been included as the kinase purity is not assured. kimberly_stegmaier@dfci.harvard.edu Accepted 2015-FEB-02 by PPORRAS Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation poly_glu-tyr POLY_GLU_TYR peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EEEY Poly GLU-TYR 4:1 M/Wt 20,000Da to 50,000Da ksyk_human Tyrosine-protein kinase SYK SYK Spleen tyrosine kinase p72-Syk protein protein human Homo sapiens Human MASSGMADSANHLPFFFGNITREEAEDYLVQGGMSDGLYLLRQSRNYLGGFALSVAHGRKAHHYTIERELNGTYAIAGGRTHASPADLCHYHSQESDGLVCLLKKPFNRPQGVQPKTGPFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGKISREESEQIVLIGSKTNGKFLIRARDNNGSYALCLLHEGKVLHYRIDKDKTGKLSIPEGKKFDTLWQLVEHYSYKADGLLRVLTVPCQKIGTQGNVNFGGRPQLPGSHPATWSAGGIISRIKSYSFPKPGHRKSSPAQGNRQESTVSFNPYEPELAPWAADKGPQREALPMDTEVYESPYADPEEIRPKEVYLDRKLLTLEDKELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHVKDKNIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRADENYYKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPAGCPREMYDLMNLCWTYDVENRPGFAAVELRLRNYYYDVVN DIP protein P43405 original sequence version: 146 EAA6BDE65881FC68 flt3_human Receptor-type tyrosine-protein kinase FLT3 FLK2 FL cytokine receptor Fetal liver kinase-2 FLT3 STK1 Fms-like tyrosine kinase 3 Stem cell tyrosine kinase 1 CD135 protein protein human Homo sapiens Human MPALARDGGQLPLLVVFSAMIFGTITNQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGCALRPQSSGTVYEAAAVEVDVSASITLQVLVDAPGNISCLWVFKHSSLNCQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNTLLYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESPAVVKKEEKVLHELFGTDIRCCARNELGRECTRLFTIDLNQTPQTTLPQLFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEMSTYSTNRTMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSEDYEIDQYEEFCFSVRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISKFCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRKPQVLAEASASQASCFSDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNMSEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDNISFYATIGVCLLFIVVLTLLICHKYKKQFRYESQLQMVQVTGSSDNEYFYVDFREYEYDLKWEFPRENLEFGKVLGSGAFGKVMNATAYGISKTGVSIQVAVKMLKEKADSSEREALMSELKMMTQLGSHENIVNLLGACTLSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYENQKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDIMSDSNYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPVDANFYKLIQNGFKMDQPFYATEEIYIIMQSCWAFDSRKRPSFPNLTSFLGCQLADAEEAMYQNVDGRVSECPHTYQNRRPFSREMDLGLLSPQAQVEDS DIP protein P36888 original sequence version: 118 flt3-syk 14 19 enzyme target enzyme target substrate substrate neutral component neutral component cytoplasmic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 18 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 2A P43405 phosphores flt3-1 14 19 putative self putative self putative self putative self n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytoplasmic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true 2Ai P36888 phosphores syk-1 14 18 putative self putative self putative self putative self n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true 2Aii P43405 phosphores flt3-syk-1 14 17 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 19 enzyme enzyme neutral component neutral component cytoplasmic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 2Aiv P36888 phosphores poly-syk 14 17 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 18 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 2Av P43405 phosphores flt3-syk-2 7 19 enzyme target enzyme target substrate substrate neutral component neutral component cytoplasmic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 18 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 2B P43405 tyr-726 O-phospho-L-tyrosine P43405 tyr-768 O-phospho-L-tyrosine P43405 tyr-842 O-phospho-L-tyrosine P43405 tyr-899 O-phospho-L-tyrosine P43405 tyr-955 O-phospho-L-tyrosine flt3-syk-3 1 18 enzyme enzyme neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 19 enzyme target enzyme target substrate substrate neutral component neutral component n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range cytoplasmic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation 2C P43405 tyr-591 O-phospho-L-tyrosine P43405 tyr-768 O-phospho-L-tyrosine P43405 tyr-842 O-phospho-L-tyrosine P43405 tyr-955 O-phospho-L-tyrosine flt3-1 1 19 putative self putative self putative self putative self cytoplasmic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true 2Ci P36888 tyr-591 O-phospho-L-tyrosine P43405 tyr-768 O-phospho-L-tyrosine P43405 tyr-842 O-phospho-L-tyrosine P43405 tyr-955 O-phospho-L-tyrosine syk-1 1 18 putative self putative self putative self putative self n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection phosphorylation phosphorylation reaction phosphorylation true 2C SYK was phosphorylated on either Tyrosine 525 or 526, or both. P43405 phosphores flt3-syk-5 5 18 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3F The artificially created SYK-TEL fusion protein (containing Full length SYK) also bound to FLT3, but the TEL-SYK fusion protein (missing SYK amino acids 1-265), did not. I dont think that strictly speaking you can call aas 1-265 a 'required to bind' region of anything, as the 2 artificial constructs are different - the TEL region is fused N term in one construct - and C term in the other. (I had thought about creating the protein SYK-TEL, but it is an artificial protein.) syk-flt3 5 18 unspecified role unspecified role bait bait P43405:p.[Arg195Gly;Arg197Leu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R G certain certain sequence position certain certain certain sequence position certain R L Wed May 02 18:26:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg195gly,arg197leu' Tue Jun 30 17:58:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg195Gly;Arg197Leu]' c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range second_sh2_domain required to bind necessary binding region required to bind range range range range 19 unspecified role unspecified role prey prey physical association physical association 3G A SYK mutant with a deletion of the nuclear localization sequence (SYK Delta NLS- sequence not stated) had an enhanced association with FLT3. syk-flt3-3 6 19 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:43:45 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'internal_tandem_repeat' The tandem repeat (W/N 51 mutation) is of sequence REYEYDL, inserted in frame between leu601 and lys602. see pmid: 23103841 and 14981546 18 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3H syk-flt3-4 6 18 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3Hi syk-flt3-5 6 18 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:44:10 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'internal_tandem_repeat' The repeat is of sequence (W/N 51 mutation) REYEYDL, inserted between leu601 and lys602. see pmid: 23103841 and 14981546 region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3Hii flt3-syk-6 12 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role bait bait physical association physical association 3I flt3-syk-7 11 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role bait bait physical association physical association 3I flt3-syk-8 15 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role bait bait physical association physical association 3I flt3-syk-9 3 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role bait bait physical association physical association 3I flt3-syk-10 9 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role bait bait undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 15:01:46 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was internal_tandem_duplication physical association physical association 3I flt3-syk-11 2 19 unspecified role unspecified role bait bait undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 15:01:56 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was internal_tandem_duplication 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association 3I flt3-syk-12 10 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role bait bait undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 15:01:55 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was internal_tandem_duplication physical association physical association 3I flt3-syk-13 4 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role bait bait undefined mutation mutation mutation certain certain sequence position certain certain certain sequence position certain Wed Jul 06 15:01:55 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was internal_tandem_duplication physical association physical association 3I flt3-syk-14 16 19 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain physical association physical association 3I weak interaction flt3-syk-15 8 18 unspecified role unspecified role prey prey possible_phospho_tyr525,try526 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role bait bait physical association physical association 3I weak interaction flt3-syk-16 13 18 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component colocalization colocalization 3J syk-flt3-6 6 18 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 3C The SYK Y130E mutant and the artificially created SYK-TEL fusion protein (containing Full length SYK) all bound to FLT3 and to FLT3-ITD. flt3-flt3 5 18 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait P36888:p.[Tyr597Ala;Tyr599Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 19:48:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr597ala,tyr599ala' Tue Jun 30 19:21:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr597Ala;Tyr599Ala]' P36888:p.[Tyr589Ala;Tyr591Ala;Tyr597Ala;Tyr599Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain Y A Fri Jul 22 10:54:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr597ala,tyr599ala,tyr589ala,tyr591ala' Wed May 02 17:13:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr589ala,tyr591ala,tyr597ala,tyr599ala' Tue Jun 30 16:18:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr589Ala;Tyr591Ala;Tyr597Ala;Tyr599Ala]' region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 3E