IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. Cancer Cell (1535-6108) 2013 imex curation Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer MEK is of rabbit origin according to one reference - Breenan -2011, but no evidence this was used here.(so this means omitting 9/10 of the paper...) Fig 2D is described as an EMSA- but I am not impressed- looks very non-standard protocol, with not much of any noticeable shift, and no other details are given, so not entered. Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer MEK is of rabbit origin according to one reference - Breenan -2011, but no evidence this was used here.(so this means omitting 9/10 of the paper...) Fig 2D is described as an EMSA- but I am not impressed- looks very non-standard protocol, with not much of any noticeable shift, and no other details are given, so not entered. Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com Accepted 2013-AUG-06 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. Cancer Cell (1535-6108) 2013 imex curation Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer MEK is of rabbit origin according to one reference - Breenan -2011, but no evidence this was used here.(so this means omitting 9/10 of the paper...) Fig 2D is described as an EMSA- but I am not impressed- looks very non-standard protocol, with not much of any noticeable shift, and no other details are given, so not entered. Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-hey8 Homo sapiens moderately differentiated papillary ovarian cystoadenocarcinoma cell line. hey8 HEY 8 Human moderately differentiated papillary ovarian cystoadenocarcinoma cell line. Established from a peritoneal deposit of a moderately differentiated papillary ovarian cystoadenocarcinoma and has been reported to be moderately resistant to chemotherapy anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer MEK is of rabbit origin according to one reference - Breenan -2011, but no evidence this was used here.(so this means omitting 9/10 of the paper...) Fig 2D is described as an EMSA- but I am not impressed- looks very non-standard protocol, with not much of any noticeable shift, and no other details are given, so not entered. Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com Accepted 2013-AUG-06 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. RAF Inhibitors Activate the MAPK Pathway by Relieving Inhibitory Autophosphorylation. Cancer Cell (1535-6108) 2013 imex curation Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer MEK is of rabbit origin according to one reference - Breenan -2011, but no evidence this was used here.(so this means omitting 9/10 of the paper...) Fig 2D is described as an EMSA- but I am not impressed- looks very non-standard protocol, with not much of any noticeable shift, and no other details are given, so not entered. Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-sw620 Human caucasian colon adenocarcinoma SW620 cells sw620 Human caucasian colon adenocarcinoma SW620 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Holderfield M., Merritt H., Chan J., Wallroth M., Tandeske L., Zhai H., Tellew J., Hardy S., Hekmat-Nejad M., Stuart D.D., McCormick F., Nagel T.E. Cancer - Interactions investigated in the context of cancer MEK is of rabbit origin according to one reference - Breenan -2011, but no evidence this was used here.(so this means omitting 9/10 of the paper...) Fig 2D is described as an EMSA- but I am not impressed- looks very non-standard protocol, with not much of any noticeable shift, and no other details are given, so not entered. Interactions involving Kinase activity have not been included as the identity and origin of the substrate "MEK" is not known. The authors did not reply to a request for further info. darrin.stuart@novartis.com Accepted 2013-AUG-06 by PPORRAS 09-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 braf_human Serine/threonine-protein kinase B-raf BRAF BRAF1 RAFB1 v-Raf murine sarcoma viral oncogene homolog B1 p94 Proto-oncogene B-Raf protein protein human Homo sapiens Human MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH DIP protein P15056 original sequence version: 182 DIP protein P15056 original sequence version: 133 DIP protein P15056 original sequence version: 147 DIP protein P15056 original sequence version: 173 DIP protein P15056 original sequence version: 178 DIP protein P15056 original sequence version: 188 DIP protein P15056 original sequence version: 193 DIP protein P15056 original sequence version: 195 DIP protein P15056 original sequence version: 146 DIP protein P15056 original sequence version: 153 0798C2AAB487E813 raf1-braf-11 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey P15056:p.Gly464Glu mutation mutation certain certain sequence position certain certain certain sequence position certain G E Wed May 02 17:26:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly464glu' Tue Jun 30 17:56:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly464Glu' physical association physical association 5C Compound A -a RAF inhibitor. raf1-braf-12 1 5 unspecified role unspecified role prey prey P15056:p.Gly464Val mutation mutation certain certain sequence position certain certain certain sequence position certain G V Wed May 02 16:54:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly464val' Tue Jun 30 18:47:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly464Val' 4 unspecified role unspecified role bait bait physical association physical association 5C Compound A -a RAF inhibitor. raf1-braf-13 3 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 6A Compound A -a RAF inhibitor. braf-raf1-25 3 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 6Ai Compound A -a RAF inhibitor.