IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Therapeutic Targeting of the Cyclin D3:CDK4/6 Complex in T Cell Leukemia. Therapeutic Targeting of the Cyclin D3:CDK4/6 Complex in T Cell Leukemia. Cancer Cell (1535-6108) 2012 imex curation Sawai C.M., Freund J., Oh P., Ndiaye-Lobry D., Bretz J.C., Strikoudis A., Genesca L., Trimarchi T., Kelliher M.A., Clark M., Soulier J., Chen-Kiang S., Aifantis I. Cancer - Interactions investigated in the context of cancer Cell lysates were IPed with anti-CDK2 and washed with Kinase buffer before kinase activity was determined. Recombinant Rb was stated to be Human in origin by the supplier - QED Bioscience. iannis.aifantis@nyumc.org 15-Nov-2012: Contacted by pporras. Only protein-protein interactions mouse-thymocyte MOUSE THYMOCYTE thymocyte THYMOCYTE anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sawai C.M., Freund J., Oh P., Ndiaye-Lobry D., Bretz J.C., Strikoudis A., Genesca L., Trimarchi T., Kelliher M.A., Clark M., Soulier J., Chen-Kiang S., Aifantis I. Cancer - Interactions investigated in the context of cancer Cell lysates were IPed with anti-CDK2 and washed with Kinase buffer before kinase activity was determined. Recombinant Rb was stated to be Human in origin by the supplier - QED Bioscience. iannis.aifantis@nyumc.org Accepted 2012-OCT-30 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation Therapeutic Targeting of the Cyclin D3:CDK4/6 Complex in T Cell Leukemia. Therapeutic Targeting of the Cyclin D3:CDK4/6 Complex in T Cell Leukemia. Cancer Cell (1535-6108) 2012 imex curation Sawai C.M., Freund J., Oh P., Ndiaye-Lobry D., Bretz J.C., Strikoudis A., Genesca L., Trimarchi T., Kelliher M.A., Clark M., Soulier J., Chen-Kiang S., Aifantis I. Cancer - Interactions investigated in the context of cancer Cell lysates were IPed with anti-CDK2 and washed with Kinase buffer before kinase activity was determined. Recombinant Rb was stated to be Human in origin by the supplier - QED Bioscience. iannis.aifantis@nyumc.org 15-Nov-2012: Contacted by pporras. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Sawai C.M., Freund J., Oh P., Ndiaye-Lobry D., Bretz J.C., Strikoudis A., Genesca L., Trimarchi T., Kelliher M.A., Clark M., Soulier J., Chen-Kiang S., Aifantis I. Cancer - Interactions investigated in the context of cancer Cell lysates were IPed with anti-CDK2 and washed with Kinase buffer before kinase activity was determined. Recombinant Rb was stated to be Human in origin by the supplier - QED Bioscience. iannis.aifantis@nyumc.org Accepted 2012-OCT-30 by PPORRAS 15-Nov-2012: Contacted by pporras. Cancer Cell (1535-6108) 2012 Only protein-protein interactions imex curation cdk6_mouse Cyclin-dependent kinase 6 Serine/threonine-protein kinase PLSTIRE Cdkn6 Cdk6 Crk2 Cell division protein kinase 6 CR2 protein kinase protein protein mouse Mus musculus Mouse MEKDSLSRADQQYECVAEIGEGAYGKVFKARDLKNGGRFVALKRVRVQTSEEGMPLSTIREVAVLRHLETFEHPNVVRLFDVCTVSRTDRETKLTLVFEHVDQDLTTYLDKVPEPGVPTETIKDMMFQLLRGLDFLHSHRVVHRDLKPQNILVTSSGQIKLADFGLARIYSFQMALTSVVVTLWYRAPEVLLQSSYATPVDLWSVGCIFAEMFRRKPLFRGSSDVDQLGKILDIIGLPGEEDWPRDVALPRQAFHSKSAQPIEKFVTDIDELGKDLLLKCLTFNPAKRISAYGALNHPYFQDLERYKDNLNSHLPSNQSTSELNTA 127B7ECFFC54C3AC rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK DIP protein P06400 original sequence version: 102 DIP protein P06400 original sequence version: 195 DIP protein P06400 original sequence version: 204 DIP protein P06400 original sequence version: 126 DIP protein P06400 original sequence version: 151 DIP protein P06400 original sequence version: 153 DIP protein P06400 original sequence version: 181 DIP protein P06400 original sequence version: 184 DIP protein P06400 original sequence version: 213 DIP protein P06400 original sequence version: 208 DIP protein P06400 original sequence version: 174 DIP protein P06400 original sequence version: 212 DIP protein P06400 original sequence version: 179 DIP protein P06400 original sequence version: 146 C8E746111E19CC32 ccnd3_mouse G1/S-specific cyclin-D3 Ccnd3 Cyl-3 protein protein mouse Mus musculus Mouse MELLCCEGTRHAPRAGPDPRLLGDQRVLQSLLRLEERYVPRASYFQCVQKEIKPHMRKMLAYWMLEVCEEQRCEEDVFPLAMNYLDRYLSCVPTRKAQLQLLGTVCLLLASKLRETTPLTIEKLCIYTDQAVAPWQLREWEVLVLGKLKWDLAAVIAHDFLALILHRLSLPSDRQALVKKHAQTFLALCATDYTFAMYPPSMIATGSIGAAVLGLGACSMSADELTELLAGITGTEVDCLRACQEQIEAALRESLREAAQTAPSPVPKAPRGSSSQGPSQTSTPTDVTAIHL 5441639A9B1B1475 cdk2_mouse Cyclin-dependent kinase 2 Cdk2 Cell division protein kinase 2 Cdkn2 protein protein mouse Mus musculus Mouse MENFQKVEKIGEGTYGVVYKAKNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINAEGSIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMHLVCTQHHAKCCGEHRRNGRHSLCPLCSYLEVAASQGGGMTAVSAPHPVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P97377 original sequence version: 140 D806BC2F150AEDFC cdk2-rb1-7 2 4 enzyme target enzyme target substrate substrate neutral component neutral component ecoli Escherichia coli (strain K12) 6 enzyme enzyme neutral component neutral component mouse-thymocyte MOUSE THYMOCYTE thymocyte THYMOCYTE phosphorylation phosphorylation reaction phosphorylation 3F, 4G CDK2-containing complexes immunoprecipitated from Ccnd3−/− total thymocytes showed little kinase activity toward exogenous Rb - as compared to controls. Ccnd3−/−Cdkn1b−/− and Ccnd3−/−Rb1−/− thymocytes showed an intermediate level of activity. cdk6-ccnd3-1 1 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 6A