IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Cancer Cell (1535-6108) 2011 imex curation Scott K.L., Nogueira C., Heffernan T.P., van Doorn R., Dhakal S., Hanna J.A., Min C., Jaskelioff M., Xiao Y., Wu C.J., Cameron L.A., Perry S.R., Zeid R., Feinberg T., Kim M., Vande Woude G., Granter S.R., Bosenberg M., Chu G.C., Depinho R.A., Rimm D.L., Chin L. Cancer - Interactions investigated in the context of cancer lynda_chin@dfci.harvard.edu 23-Sept-2011: Contacted by JYOTI. jyoti Only protein-protein interactions human-wm115 Human WM115 Melanoma cell line wm115 WM115 Human melanoma cell line http://www.lgcstandards-atcc.org/LGCAdvancedCatalogueSearch/ProductDescription/tabid/1068/Default.aspx?ATCCNum=CRL-1675&Template=cellBiology anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Scott K.L., Nogueira C., Heffernan T.P., van Doorn R., Dhakal S., Hanna J.A., Min C., Jaskelioff M., Xiao Y., Wu C.J., Cameron L.A., Perry S.R., Zeid R., Feinberg T., Kim M., Vande Woude G., Granter S.R., Bosenberg M., Chu G.C., Depinho R.A., Rimm D.L., Chin L. Cancer - Interactions investigated in the context of cancer lynda_chin@dfci.harvard.edu Accepted 2011-AUG-03 by JYOTI 23-Sept-2011: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 jyoti Only protein-protein interactions imex curation Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Proinvasion metastasis drivers in early-stage melanoma are oncogenes. Cancer Cell (1535-6108) 2011 imex curation Scott K.L., Nogueira C., Heffernan T.P., van Doorn R., Dhakal S., Hanna J.A., Min C., Jaskelioff M., Xiao Y., Wu C.J., Cameron L.A., Perry S.R., Zeid R., Feinberg T., Kim M., Vande Woude G., Granter S.R., Bosenberg M., Chu G.C., Depinho R.A., Rimm D.L., Chin L. Cancer - Interactions investigated in the context of cancer lynda_chin@dfci.harvard.edu 23-Sept-2011: Contacted by JYOTI. jyoti Only protein-protein interactions human-hmel468 Human HMEL468 hmel468 HMEL468 (PMEL/hTERT/CDK4(R24C)/p53DD), a TERT-immortalized primary human melanocyte line engineered to express BRAFV600E (Garraway et al., 2005). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Scott K.L., Nogueira C., Heffernan T.P., van Doorn R., Dhakal S., Hanna J.A., Min C., Jaskelioff M., Xiao Y., Wu C.J., Cameron L.A., Perry S.R., Zeid R., Feinberg T., Kim M., Vande Woude G., Granter S.R., Bosenberg M., Chu G.C., Depinho R.A., Rimm D.L., Chin L. Cancer - Interactions investigated in the context of cancer lynda_chin@dfci.harvard.edu Accepted 2011-AUG-03 by JYOTI 23-Sept-2011: Contacted by JYOTI. Cancer Cell (1535-6108) 2011 jyoti Only protein-protein interactions imex curation paxi_human Paxillin PXN protein protein human Homo sapiens Human MDDLDALLADLESTTSHISKRPVFLSEETPYSYPTGNHTYQEIAVPPPVPPPPSSEALNGTILDPLDQWQPSSSRFIHQQPQSSSPVYGSSAKTSSVSNPQDSVGSPCSRVGEEEHVYSFPNKQKSAEPSPTVMSTSLGSNLSELDRLLLELNAVQHNPPGFPADEANSSPPLPGALSPLYGVPETNSPLGGKAGPLTKEKPKRNGGRGLEDVRPSVESLLDELESSVPSPVPAITVNQGEMSSPQRVTSTQQQTRISASSATRELDELMASLSDFKIQGLEQRADGERCWAAGWPRDGGRSSPGGQDEGGFMAQGKTGSSSPPGGPPKPGSQLDSMLGSLQSDLNKLGVATVAKGVCGACKKPIAGQVVTAMGKTWHPEHFVCTHCQEEIGSRNFFERDGQPYCEKDYHNLFSPRCYYCNGPILDKVVTALDRTWHPEHFFCAQCGAFFGPEGFHEKDGKAYCRKDYFDMFAPKCGGCARAILENYISALNTLWHPECFVCRECFTPFVNGSFFEHDGQPYCEVHYHERRGSLCSGCQKPITGRCITAMAKKFHPEHFVCAFCLKQLNKGTFKEQNDKPYCQNCFLKLFC DIP protein P49023 original sequence version: 115 DIP protein P49023 original sequence version: 126 DIP protein P49023 original sequence version: 169 DIP protein P49023 original sequence version: 140 DIP protein P49023 original sequence version: 175 ABF6C0BE5939623F fak1_human Focal adhesion kinase 1 PTK2 FAK FAK1 pp125FAK Protein-tyrosine kinase 2 Focal adhesion kinase-related nonkinase Protein phosphatase 1 regulatory subunit 71 p125FAK protein protein human Homo sapiens Human MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH D8A4C15138AB0243 pxn-ptk2-3 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association Fig 6C A faint band can be seen, the intensity of which is reduced In the presence of overexpressed ACP5. pxn-ptk2-4 2 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Fig 6C ptk2-pxn-1 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig 6C ptk2-pxn-2 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig 6C