I2D I2D A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. Oncogene (0950-9232) 2011 imex curation Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-hep_3b Homo sapiens hepatocellular carcinoma (HCC) cells (p53-null). hep_3b human hepatocellular carcinoma (HCC) cells (p53-null). anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk Accepted 2012-JAN-24 by MGT Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. Oncogene (0950-9232) 2011 imex curation Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-hkcl-4 Human hepatocellular carcinoma hkcl-4 Human hepatocellular carcinoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk Accepted 2012-JAN-24 by MGT 14-Mar-2012: Contacted by JYOTI. Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. A novel interplay between oncogenic PFTK1 protein kinase and tumor suppressor TAGLN2 in the control of liver cancer cell motility. Oncogene (0950-9232) 2011 imex curation Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-l02 Immortal human liver cell line l02 Immortal human liver cell line Cell Bank No. GNHu6, China anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Leung W.K., Ching A.K., Chan A.W., Poon T.C., Mian H., Wong A.S., To K.F., Wong N. Cancer - Interactions investigated in the context of cancer natwong@cuhk.edu.hk Accepted 2012-JAN-24 by MGT 14-Mar-2012: Contacted by JYOTI. Oncogene (0950-9232) 2011 Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database tagl2_human Transgelin-2 TAGLN2 KIAA0120 SM22-alpha homolog CDABP0035 Epididymis tissue protein Li 7e protein protein human Homo sapiens Human MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL 07DFC1652CE2383D actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF DIP protein P60709 original sequence version: null DIP protein P60709 original sequence version: 63 DIP protein P60709 original sequence version: 128 DIP protein P60709 original sequence version: 129 DIP protein P60709 original sequence version: 58 DIP protein P60709 original sequence version: 66 DIP protein P60709 original sequence version: 88 DIP protein P60709 original sequence version: 89 DIP protein P60709 original sequence version: 86 DIP protein P60709 original sequence version: 138 DIP protein P60709 original sequence version: 112 6AFD05CA94E360E2 tagln2-actb-1 2 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey physical association physical association 4cii Knockdown of PFTK1 results in increased association of TAGLN2 with ACTB. tagln2-actb-2 3 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4ciii PFTK1 expression results in decreased association of TAGLN2 with ACTB. tagln2-actb-4 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait P37802:p.Ser163Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:16:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser163asp' Tue Jun 30 19:24:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser163Asp' P37802:p.Ser83Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S D Wed May 02 16:59:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser83asp' Tue Jun 30 20:25:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser83Asp' P37802:p.Ser83Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:43:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser83ala' Tue Jun 30 17:37:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser83Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P37802:p.Ser163Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:24:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser163ala' Tue Jun 30 19:41:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser163Ala' physical association physical association 4ci, 7ai, 7bi Knockdown of PFTK1 with sh-RNA results in increased association of TAGLN2 with ACTB. Phospho-defective mutants S83A and S163A mutants abrogated the inhibitory effect of PFTK1. Phospho-mimetic mutants S83D and S163D mutants decreased Actin binding in PFTK-suppressed Hep3B cells.