I2D I2D hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. Oncogene (0950-9232) 2011 imex curation Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). confocal microscopy confocal microscopy immunostaining immunostaining Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. Oncogene (0950-9232) 2011 imex curation Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. Oncogene (0950-9232) 2011 imex curation Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. Oncogene (0950-9232) 2011 imex curation Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. hSSB1 binds and protects p21 from ubiquitin-mediated degradation and positively correlates with p21 in human hepatocellular carcinomas. Oncogene (0950-9232) 2011 imex curation Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xu S., Feng Z., Zhang M., Wu Y., Sang Y., Xu H., Lv X., Hu K., Cao J., Zhang R., Chen L., Liu M., Yun JP., Zeng YX., Kang T. Cancer - Interactions investigated in the context of cancer kangtb@mail.sysu.edu.cn Accepted 2011-MAY-23 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation cdn1a_human Cyclin-dependent kinase inhibitor 1 CDKN1A CAP20 CDKN1 CIP1 MDA6 PIC1 SDI1 WAF1 p21 CDK-interacting protein 1 Melanoma differentiation-associated protein 6 protein protein human Homo sapiens Human MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP DIP protein P38936 original sequence version: 120 DIP protein P38936 original sequence version: 165 DIP protein P38936 original sequence version: 160 98D1E7C519ADFCA9 sosb1_human SOSS complex subunit B1 NABP2 Nucleic acid-binding protein 2 OBFC2B Oligonucleotide/oligosaccharide-binding fold-containing protein 2B SSB1 Sensor of single-strand DNA complex subunit B1 Sensor of ssDNA subunit B1 Single-stranded DNA-binding protein 1 LP3587 protein protein human Homo sapiens Human MTTETFVKDIKPGLKNLNLIFIVLETGRVTKTKDGHEVRTCKVADKTGSINISVWDDVGNLIQPGDIIRLTKGYASVFKGCLTLYTGRGGDLQKIGEFCMVYSEVPNFSEPNPEYSTQQAPNKAVQNDSNPSASQPTTGPSAASPASENQNGNGLSAPPGPGGGPHPPHTPSHPPSTRITRSQPNHTPAGPPGPSSNPVSNGKETRRSSKR B72014397B7947E2 cdkn1a-obfc2b 5 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2a obfc2b-cdkn1a 5 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2a cdkn1a-obfc2b-1 1 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 2b obfc2b-cdkn1a-1 3 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 2c cdkn1a-obfc2b-2 4 7 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 2d cdkn1a-obfc2b-3 2 7 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3d,e,f obfc2b-cdkn1a-2 2 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3a,b,c