Molecular Connections Molecular Connections Pvt. Ltd. 2/2, Kariappa Road, Basavanagudi, Bangalore -560 004 www.molecularconnections.com Modulation of extracellular matrix/adhesion molecule expression by BRG1 is associated with increased melanoma invasiveness. Modulation of extracellular matrix/adhesion molecule expression by BRG1 is associated with increased melanoma invasiveness. Mol. Cancer 2010 imex curation Saladi SV., Keenen B., Marathe HG., Qi H., Chin KV., de la Serna IL. Cancer - Interactions investigated in the context of cancer You did not add the cross linking method as an Exp. Mod. No flag tag added for BRG1. Only one of the 2 interactions is entered as an "association". No comment has been entered as to the region of nucleic acid bound - I have done this for you --we cannot add this info as a feature currently. Formaldehyde cross-linking of Protein-DNA ivana.delaserna@utoledo.edu 21-Dec-2010: Contacted by JYOTI. mgt Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd human-sk_mel_5 Homo sapiens sk_mel_5 human sk-mel-5 coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Saladi SV., Keenen B., Marathe HG., Qi H., Chin KV., de la Serna IL. Cancer - Interactions investigated in the context of cancer You did not add the cross linking method as an Exp. Mod. No flag tag added for BRG1. Only one of the 2 interactions is entered as an "association". No comment has been entered as to the region of nucleic acid bound - I have done this for you --we cannot add this info as a feature currently. Formaldehyde cross-linking of Protein-DNA Species detail of the Flag tagged BRG1 obtained from author ivana.delaserna@utoledo.edu Accepted 2010-DEC-24 by MGT. 21-Dec-2010: Contacted by JYOTI. Mol. Cancer 2010 mgt Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd Modulation of extracellular matrix/adhesion molecule expression by BRG1 is associated with increased melanoma invasiveness. Modulation of extracellular matrix/adhesion molecule expression by BRG1 is associated with increased melanoma invasiveness. Mol. Cancer 2010 imex curation Saladi SV., Keenen B., Marathe HG., Qi H., Chin KV., de la Serna IL. Cancer - Interactions investigated in the context of cancer You did not add the cross linking method as an Exp. Mod. No flag tag added for BRG1. Only one of the 2 interactions is entered as an "association". No comment has been entered as to the region of nucleic acid bound - I have done this for you --we cannot add this info as a feature currently. Formaldehyde cross-linking of Protein-DNA ivana.delaserna@utoledo.edu 21-Dec-2010: Contacted by JYOTI. mgt Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd human-sk_mel_5 Homo sapiens sk_mel_5 human sk-mel-5 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Saladi SV., Keenen B., Marathe HG., Qi H., Chin KV., de la Serna IL. Cancer - Interactions investigated in the context of cancer You did not add the cross linking method as an Exp. Mod. No flag tag added for BRG1. Only one of the 2 interactions is entered as an "association". No comment has been entered as to the region of nucleic acid bound - I have done this for you --we cannot add this info as a feature currently. Formaldehyde cross-linking of Protein-DNA ivana.delaserna@utoledo.edu Accepted 2010-DEC-24 by MGT. 21-Dec-2010: Contacted by JYOTI. Mol. Cancer 2010 mgt Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd smca4_human Transcription activator BRG1 SMARCA4 BRG1 SNF2B SNF2L4 ATP-dependent helicase SMARCA4 SNF2-beta BAF190A Mitotic growth and transcription activator Protein BRG-1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 Protein brahma homolog 1 BRG1-associated factor 190A protein protein human Homo sapiens Human MSTPDPPLGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHSMMGPSPGPPSAGHPIPTQGPGGYPQDNMHQMHKPMESMHEKGMSDDPRYNQMKGMGMRSGGHAGMGPPPSPMDQHSQGYPSPLGGSEHASSPVPASGPSSGPQMSSGPGGAPLDGADPQALGQQNRGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQQMPTLPPPSVSATGPGPGPGPGPGPGPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGPPKPWPEGPMANAAAPTSTPQKLIPPQPTGRPSPAPPAVPPAASPVMPPQTQSPGQPAQPAPMVPLHQKQSRITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKATIELKALRLLNFQRQLRQEVVVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVTGKIQKLTKAVATYHANTEREQKKENERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTELVRQHKAAQVAKEKKKKKKKKKAENAEGQTPAIGPDGEPLDETSQMSDLPVKVIHVESGKILTGTDAPKAGQLEAWLEMNPGYEVAPRSDSEESGSEEEEEEEEEEQPQAAQPPTLPVEEKKKIPDPDSDDVSEVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDESRHCSTGSGSASFAHTAPPPAGVNPDLEEPPLKEEDEVPDDETVNQMIARHEEEFDLFMRMDLDRRREEARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKMFGRGSRHRKEVDYSDSLTEKQWLKAIEEGTLEEIEEEVRQKKSSRKRKRDSDAGSSTPTTSTRSRDKDDESKKQKKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSSGRQLSEVFIQLPSRKELPEYYELIRKPVDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFTSVRQKIEKEDDSEGEESEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPSRGSRAKPVVSDDDSEEEQEEDRSGSGSEED DIP protein P51532 original sequence version: 125 DIP protein P51532 original sequence version: 115 DIP protein P51532 original sequence version: 154 DIP protein P51532 original sequence version: 136 DIP protein P51532-1 original sequence version: 136 ABDA5EDBF10D7D28 mmp2_human_gene 72 kDa type IV collagenase gene gene gene human Homo sapiens Human sp1_human Transcription factor Sp1 SP1 TSFP1 protein protein human Homo sapiens Human MSDQDHSMDEMTAVVKIEKGVGGNNGGNGNGGGAFSQARSSSTGSSSSTGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGSSTNGSNGSESSKNRTVSGGQYVVAAAPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIITNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAVTISSSGSQESGSQPVTSGTTISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTTSGSSGTNSQGQTPQRVSGLQGSDALNIQQNQTSGGSLQAGQQKEGEQNQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVPNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTRREACTCPYCKDSEGRGSGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCTWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGSGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVADLQSINISGNGF DIP protein P08047 original sequence version: 185 DIP protein P08047 original sequence version: 187 DIP protein P08047 original sequence version: 181 DIP protein P08047 original sequence version: 162 DIP protein P08047 original sequence version: 161 43893DBF6518B9EA brg1-mmp2-1 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association fig. 7d BRG1 was significantly enriched on the MMP2 promoter compared to the control CD25 region. SP1 binding to the MMP2 promoter was significantly increased in SK+MEL5+BRG1 as compared to control. sp1-mmp2-1 2 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait association association fig. 7e Brg1 BRG1 was significantly enriched on the MMP2 promoter compared to the control CD25 region. SP1 binding to the MMP2 promoter was significantly increased in SK+MEL5+BRG1 as compared to control cells. brg1-sp1-1 1 3 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey physical association physical association fig. 7c