IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Store-independent activation of Orai1 by SPCA2 in mammary tumors. Store-independent activation of Orai1 by SPCA2 in mammary tumors. Cell (0092-8674) 2010 imex curation Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down anti tag western anti tag western blot anti tag western Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Store-independent activation of Orai1 by SPCA2 in mammary tumors. Store-independent activation of Orai1 by SPCA2 in mammary tumors. Cell (0092-8674) 2010 imex curation Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer Source of ORAI1 described in PMID: 18995841 rrao@jhmi.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Store-independent activation of Orai1 by SPCA2 in mammary tumors. Store-independent activation of Orai1 by SPCA2 in mammary tumors. Cell (0092-8674) 2010 imex curation Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer Cells were biotin labeled and co-immunoprecipitation was performed using anti-SPCA2 antibody. Biotin-labeled proteins were enriched by neutravidin beads. It is unclear whether anti-bait or anti-tab Abs were used in the final western blot. Source of ORAI1 described in PMID: 18995841 rrao@jhmi.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Store-independent activation of Orai1 by SPCA2 in mammary tumors. Store-independent activation of Orai1 by SPCA2 in mammary tumors. Cell (0092-8674) 2010 imex curation Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer Source of ORAI1 described in PMID: 18995841 rrao@jhmi.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Store-independent activation of Orai1 by SPCA2 in mammary tumors. Store-independent activation of Orai1 by SPCA2 in mammary tumors. Cell (0092-8674) 2010 imex curation Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Store-independent activation of Orai1 by SPCA2 in mammary tumors. Store-independent activation of Orai1 by SPCA2 in mammary tumors. Cell (0092-8674) 2010 imex curation Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy anti tag immunost anti tag immunostaining anti tag immunost Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer Fluorescence-tagged proteins visualised by tag-fluorescence Source of STIM1 described in PMID: 16906149 rrao@jhmi.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation Store-independent activation of Orai1 by SPCA2 in mammary tumors. Store-independent activation of Orai1 by SPCA2 in mammary tumors. Cell (0092-8674) 2010 imex curation Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Feng M., Grice DM., Faddy HM., Nguyen N., Leitch S., Wang Y., Muend S., Kenny PA., Sukumar S., Roberts-Thomson SJ., Monteith GR., Rao R. Cancer - Interactions investigated in the context of cancer rrao@jhmi.edu Accepted 2010-Not a month!-2 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation stim1_human Stromal interaction molecule 1 STIM1 GOK protein protein human Homo sapiens Human MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQMSRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELSPSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK DIP protein Q13586 original sequence version: 141 DIP protein Q13586 original sequence version: 99 DIP protein Q13586 original sequence version: 108 DIP protein Q13586 original sequence version: 121 DIP protein Q13586 original sequence version: 125 DIP protein Q13586 original sequence version: 100 FFB5E7CB3D68A7A6 atp2c1_c2_c1 ATP2C1/ATP2C2 C1 chimera protein protein human Homo sapiens Human MKVARFQKIPNGENETMIPVLTSKKASELPVSEVASILQADLQNGLNKCEVSHRRAFHGWNEFDISEDEPLWKKYISQFKNPLIMLLLASAVISVLMHQFDDAVSITVAILIVVTVAFVQEYRSEKSLEELSKLVPPECHCVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLFEAVDLSIDESSLTGETTPCSKVTAPQPAATNGDLASRSNIAFMGTLVRCGKAKGVVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGWLLGKDILEMFTISVSLAVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTLTKNEMTVTHIFTSDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTLMGKPTEGALIALAMKMGLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCTTYQSKGQTLTLTQQQRDVYQQEKARMGSAGLRVLALASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIASGVSIKMITGDSQETAVAIASRLGLYSKTSQSVSGEEIDAMDVQQLSQIVPKVAVFYRASPRHKMKIIKSLQKNGSVVAMTGDGVNDAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKNFVRFQLSTSIAALTLISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSILTKNLILKILVSSIIIVCGTLFVFWRELRDNV An amino acid sequence which is not represented in UniProt 2945B140CBC731FB atp2c1_c2_n2 ATP2C1/ATP2C2 N2 chimera protein protein human Homo sapiens Human MVEGRVSEFLKKLGFSGGGRQYQALEKDEEEALIDEQSELKAIEKEKKVTALPPKEACKCQKEDLARAFCVDLHTGLSEFSVTQRRLAHGWNEFVADSQFKNPLIMLLLASAVISVLMHQFDDAVSITVAILIVVTVAFVQEYRSEKSLEELSKLVPPECHCVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLFEAVDLSIDESSLTGETTPCSKVTAPQPAATNGDLASRSNIAFMGTLVRCGKAKGVVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGWLLGKDILEMFTISVSLAVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTLTKNEMTVTHIFTSDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTLMGKPTEGALIALAMKMGLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCTTYQSKGQTLTLTQQQRDVYQQEKARMGSAGLRVLALASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIASGVSIKMITGDSQETAVAIASRLGLYSKTSQSVSGEEIDAMDVQQLSQIVPKVAVFYRASPRHKMKIIKSLQKNGSVVAMTGDGVNDAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKNFVRFQLSTSIAALTLISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSILTKNLILKILVSSIIIVCGTLFVFWRELRDNVITPRDTTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCSNRMFCYAVLGSIMGQLLVIYFPPLQKVFQTESLSILDLLFLLGLTSSVCIVAEIIKKVERSREKIQKHVSSTSSSFLEV An amino acid sequence which is not represented in UniProt ECCAC3073B73485B crcm1_human Calcium release-activated calcium channel protein 1 ORAI1 CRACM1 TMEM142A Protein orai-1 Transmembrane protein 142A protein protein human Homo sapiens Human MHPEPAPPPSRSSPELPPSGGSTTSGSRRSRRRSGDGEPPGAPPPPPSAVTYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDADHDYPPGLLIAFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKKQPGQPRPTSKPPASGAAANVSTSGITPGQAAAIASTTIMVPFGLIFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHPLTPGSHYA DIP protein Q96D31 original sequence version: 95 DIP protein Q96D31 original sequence version: 38 DIP protein Q96D31 original sequence version: 58 DIP protein Q96D31 original sequence version: 55 DIP protein Q96D31 original sequence version: 57 DIP protein Q96D31 original sequence version: 53 DIP protein Q96D31 original sequence version: 63 DIP protein Q96D31 original sequence version: 76 DIP protein Q96D31 original sequence version: 79 DIP protein Q96D31 original sequence version: 56 F85F7C06F36628D4 at2c2_human Calcium-transporting ATPase type 2C member 2 ATP2C2 KIAA0703 SPCA2 Ca(2+)/Mn(2+)-ATPase 2C2 Secretory pathway Ca(2+)-transporting ATPase type 2 protein protein human Homo sapiens Human MVEGRVSEFLKKLGFSGGGRQYQALEKDEEEALIDEQSELKAIEKEKKVTALPPKEACKCQKEDLARAFCVDLHTGLSEFSVTQRRLAHGWNEFVADNSEPVWKKYLDQFKNPLILLLLGSALVSVLTKEYEDAVSIATAVLVVVTVAFIQEYRSEKSLEELTKLVPPECNCLREGKLQHLLARELVPGDVVSLSIGDRIPADIRLTEVTDLLVDESSFTGEAEPCSKTDSPLTGGGDLTTLSNIVFMGTLVQYGRGQGVVIGTGESSQFGEVFKMMQAEETPKTPLQKSMDRLGKQLTLFSFGIIGLIMLIGWSQGKQLLSMFTIGVSLAVAAIPEGLPIVVMVTLVLGVLRMAKKRVIVKKLPIVETLGCCSVLCSDKTGTLTANEMTVTQLVTSDGLRAEVSGVGYDGQGTVCLLPSKEVIKEFSNVSVGKLVEAGCVANNAVIRKNAVMGQPTEGALMALAMKMDLSDIKNSYIRKKEIPFSSEQKWMAVKCSLKTEDQEDIYFMKGALEEVIRYCTMYNNGGIPLPLTPQQRSFCLQEEKRMGSLGLRVLALASGPELGRLTFLGLVGIIDPPRVGVKEAVQVLSESGVSVKMITGDALETALAIGRNIGLCNGKLQAMSGEEVDSVEKGELADRVGKVSVFFRTSPKHKLKIIKALQESGAIVAMTGDGVNDAVALKSADIGIAMGQTGTDVSKEAANMILVDDDFSAIMNAVEEGKGIFYNIKNFVRFQLSTSISALSLITLSTVFNLPSPLNAMQILWINIIMDGPPAQSLGVEPVDKDAFRQPPRSVRDTILSRALILKILMSAAIIISGTLFIFWKEMPEDRASTPRTTTMTFTCFVFFDLFNALTCRSQTKLIFEIGFLRNHMFLYSVLGSILGQLAVIYIPPLQRVFQTENLGALDLLFLTGLASSVFILSELLKLCEKYCCSPKRVQMHPEDV E270F10EAAD185E3 at2c1_human Calcium-transporting ATPase type 2C member 1 ATP2C1 KIAA1347 PMR1L ATP-dependent Ca(2+) pump PMR1 Ca(2+)/Mn(2+)-ATPase 2C1 Secretory pathway Ca(2+)-transporting ATPase type 1 HUSSY-28 protein protein human Homo sapiens Human MKVARFQKIPNGENETMIPVLTSKKASELPVSEVASILQADLQNGLNKCEVSHRRAFHGWNEFDISEDEPLWKKYISQFKNPLIMLLLASAVISVLMHQFDDAVSITVAILIVVTVAFVQEYRSEKSLEELSKLVPPECHCVREGKLEHTLARDLVPGDTVCLSVGDRVPADLRLFEAVDLSIDESSLTGETTPCSKVTAPQPAATNGDLASRSNIAFMGTLVRCGKAKGVVIGTGENSEFGEVFKMMQAEEAPKTPLQKSMDLLGKQLSFYSFGIIGIIMLVGWLLGKDILEMFTISVSLAVAAIPEGLPIVVTVTLALGVMRMVKKRAIVKKLPIVETLGCCNVICSDKTGTLTKNEMTVTHIFTSDGLHAEVTGVGYNQFGEVIVDGDVVHGFYNPAVSRIVEAGCVCNDAVIRNNTLMGKPTEGALIALAMKMGLDGLQQDYIRKAEYPFSSEQKWMAVKCVHRTQQDRPEICFMKGAYEQVIKYCTTYQSKGQTLTLTQQQRDVYQQEKARMGSAGLRVLALASGPELGQLTFLGLVGIIDPPRTGVKEAVTTLIASGVSIKMITGDSQETAVAIASRLGLYSKTSQSVSGEEIDAMDVQQLSQIVPKVAVFYRASPRHKMKIIKSLQKNGSVVAMTGDGVNDAVALKAADIGVAMGQTGTDVCKEAADMILVDDDFQTIMSAIEEGKGIYNNIKNFVRFQLSTSIAALTLISLATLMNFPNPLNAMQILWINIIMDGPPAQSLGVEPVDKDVIRKPPRNWKDSILTKNLILKILVSSIIIVCGTLFVFWRELRDNVITPRDTTMTFTCFVFFDMFNALSSRSQTKSVFEIGLCSNRMFCYAVLGSIMGQLLVIYFPPLQKVFQTESLSILDLLFLLGLTSSVCIVAEIIKKVERSREKIQKHVSSTSSSFLEV 4C1495D58FDA7EA1 atp2c2-orai1-1 5 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association Coimmunoprecipitation of endogenous SPCA2 and Orai1 in MCF-7 cells. Fig. 4C atp2c2-orai1-2 3 12 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association We also used cell-surface biotinylation to confirm that a portion of SPCA2-complexed Orai1 was found at the plasma membrane (Figures S5B and S5C). Supp. Fig. 5B stim1-atp2c1-1 6 8 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization confocal micrographs showing that GFP-SPCA1 and Myc-STIM1 partially colocalized in HEK293 cells atp2c2-orai1-3 2 11 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Coimmunoprecipitation of HA-SPCA with Orai1-Myc following expression in HEK293. Fig. 4D atp2c2-orai1-4 6 12 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Confocal micrographs of indirect immunofluorescence from Myc-SPCA2 and HA-Orai1 expressed in HEK293 cells showing colocalization. Supp. Fig. 5D atp2c2-orai1-5 4 12 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Coimmunoprecipitation of Orai1 with SPCA2 in untreated or TG-treated HEK293 cells. Supp. Fig. 5D Interaction between SPCA2 and Orai1 was not affected by ER Ca2+ store depletion by thapsigargin. atp2c1_c2_n2-orai1 7 11 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5B atp2c1_c2_c1-orai1 7 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5B atp2c2-orai1-6 1 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O75185:p.Ser78Xaa mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S X Fri Jul 22 11:15:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser78unk' Wed May 02 18:43:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser78xaa' Tue Jun 30 15:42:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser78Xaa' O75185:p.Val71Xaa mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V X Fri Jul 22 10:53:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val71unk' Wed May 02 16:46:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val71xaa' Tue Jun 30 20:03:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val71Xaa' O75185:p.Thr75Xaa mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T X Fri Jul 22 11:04:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'thr75unk' Wed May 02 20:32:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr75xaa' Tue Jun 30 16:11:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr75Xaa' O75185:p.Val95Xaa mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V X Fri Jul 22 10:53:31 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val95unk' Wed May 02 17:22:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val95xaa' Tue Jun 30 16:24:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val95Xaa' 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association GST-SPCA1/2 N-terminal fragments were coexpressed with HA-Orai1 in HEK293 cells, and interaction with Orai1 was examined by GST pull-down. Fig. 5D, 5E, 5F, 5G Identity of residues to which mutations were altered is not given in text. atp2c2-orai1-7 1 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q96D31:p.Leu273Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L S Wed May 02 16:06:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu273ser' Tue Jun 30 19:48:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu273Ser' n2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Supp. Fig. 6E, 6G atp2c2-orai1-8 1 12 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n1 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Supp. Fig. 6F atp2c2-orai1-9 6 12 unspecified role unspecified role neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Confocal micrographs of indirect immunofluorescence from Myc-SPCA2 and HA-Orai1 expressed in HEK293 cells showing colocalization. Fig. 7B atp2c2-orai1-10 1 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 7C