I2D I2D Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Oncogene (0950-9232) 2011 imex curation Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down autoradiography autoradiography Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it Accepted 2011-MAY-11 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Oncogene (0950-9232) 2011 imex curation Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it Accepted 2011-MAY-11 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Oncogene (0950-9232) 2011 imex curation Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it Accepted 2011-MAY-11 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Oncogene (0950-9232) 2011 imex curation Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it Accepted 2011-MAY-11 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Numb activates the E3 ligase Itch to control Gli1 function through a novel degradation signal. Oncogene (0950-9232) 2011 imex curation Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it 26-Jun-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Di Marcotullio L., Greco A., Mazza D., Canettieri G., Pietrosanti L., Infante P., Coni S., Moretti M., De Smaele E., Ferretti E., Screpanti I., Gulino A. Apoptosis - Interactions involving proteins with a function related to apoptosis Author confirms that Itch is of human origin and Numb is of mouse origin. The Itch protein described matches isoform 2 of Q96J02. The murine Numb described matches Murine Numb-Like Protein O08919. alberto.gulino@uniroma1.it Accepted 2011-MAY-11 by ORCHARD 26-Jun-2011: Contacted by JYOTI. Oncogene (0950-9232) 2011 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database itch_mouse E3 ubiquitin-protein ligase Itchy Itch HECT-type E3 ubiquitin transferase Itchy homolog protein protein mouse Mus musculus Mouse MSDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPTSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNTSTSEGATSGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE DIP protein Q8C863 original sequence version: 44 905FDBE00A1EA7EA itch_human E3 ubiquitin-protein ligase Itchy homolog HECT-type E3 ubiquitin transferase Itchy homolog Atrophin-1-interacting protein 4 NFE2-associated polypeptide 1 ITCH protein protein human Homo sapiens Human MSDSGSQLGSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHFRVWSHQTLKSDVLLGTAALDIYETLKSNNMKLEEVVVTLQLGGDKEPTETIGDLSICLDGLQLESEVVTNGETTCSENGVSLCLPRLECNSAISAHCNLCLPGLSDSPISASRVAGFTGASQNDDGSRSKDETRVSTNGSDDPEDAGAGENRRVSGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTSEGATSGLIIPLTISGGSGPRPLNPVTQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE DIP protein Q96J02 original sequence version: 130 DIP protein Q96J02-1 original sequence version: 125 6777A2043C7B67BC gli1_human Zinc finger protein GLI1 GLI1 GLI Glioma-associated oncogene Oncogene GLI protein protein human Homo sapiens Human MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA E29B11D1A6CD3D91 q9qzs3-2 Protein numb homolog p66 66 kDa Numb protein protein mouse Mus musculus Mouse MNKLRQSFRRKKDVYVPEASRPHQWQTDEEGVRTGKCSFPVKYLGHVEVDESRGMHICEDAVKRLKAERKFFKGFFGKTGKKAVKAVLWVSADGLRVVDEKTKDLIVDQTIEKVSFCAPDRNFDRAFSYICRDGTTRRWICHCFMAVKDTGERLSHAVGCAFAACLERKQKREKECGVTATFDASRTTFTREGSFRVTTATEQAEREEIMKQLQDAKKAETDKTVVGPSVAPGNTAPSPSSPTSPTPDGTASSEMNNPHAIPRRHAPIEQLARQGSFRGFPALSQKMSPFKRQLSLRINELPSTMQRKTDFPIKNTVPEVEGEAESISSLCSQITSAFSTPSEDPFSSAPMTKPVTLVAPQSPVLQGTEWGQSSGAASPGLFQAGHRRTPSEADRWLEEVSKSVRAQQPQVSAAPLQPVLQPPPPAAIAPPAPPFQGHAFLTSQPVPVGVVPPLQPAFVPTQSYPVANGMPYPASNVPVVGITPSQMVANVFGTAGHPQTTHPHQSPSLAKQQTFPQYETSSATTSPFFKPPAQHLNGSAAFNGVDNGGLASGNRHAEVPPGTCPVDPFEAQWAALESKSKQRTNPSPTNPFSSDLQKTFEIEL 709C36BE4DEF6AE0 gli1_mouse Zinc finger protein GLI1 Gli1 Gli Glioma-associated oncogene homolog protein protein mouse Mus musculus Mouse MFNPMTPPQVNSYSEPCCLRPLHSQGVPSMGTEGLSGLPFCHQANFMSGSQGYGAARETSSCTEGSLFPPPPPPRSSVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPPQMSHQKGTSPPYGVQPCVPHDSTRGSMMLHPQSRGPRATCQLKSELDMMVGKCPEDPLEGDMSSPNSTGTQDHLLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQPLSTVEPKREREGGSGREESRLTVPESAMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLSSLDEGPCVSATGLSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSMSSAYTVSRRSSLASPFPPGTPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRMGGLSVPPWRSRTEYPGYNPNAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHHPTSVYSPQPPSITENVAMDTRGLQEEPEVGTSVMGNGLNPYMDFSSTDTLGYGGPEGTAAEPYEARGPGSLPLGPGPPTNYGPGHCAQQVSYPDPTPENWGEFPSHAGVYPSNKAPGAAYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGSPGPQPLFSHHPQLPQPQYPQSGPYPQPPHGYLSTEPRLGLNFNPSSSHSTGQLKAQLVCNYVQSQQELLWEGRNRGGLPNQELPYQSPKFLGGSQVSQSPAKTPAAAAAAYGSGFAPASANHKSGSYPAPSPCHETFTVGVNRPSHRPAAPPRLLPPLSPCYGPLKVGDTNPSCGHPEVGRLGAGPALYPPPEGQVCNALDSLDLDNTQLDFVAILDEAQGLSPPLSHEQGDSSKNTPSPSGPPNMAVGNMSVLLGSLPGETQFLNSSA 8A83B254DCBB9BDC itch-numb 2 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association 1b gli1-itch-3 5 6 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 2a itch-gli1 2 8 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain physical association physical association 2c itch-gli1-1 1 8 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction 2b, d gli1-itch-2 3 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P08151:p.[Tyr859Phe;Tyr872Phe;Ser1060Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain Y F certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:59:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr859phe,tyr872phe,ser1060ala' Tue Jun 30 19:58:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Tyr859Phe;Tyr872Phe;Ser1060Ala]' physical association physical association 2e, f itch-gli1-2 3 7 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2 numb-gli1 1 8 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 5a, S4b numb-gli1-1 3 8 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 5c,d,e numb-itch 3 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1c,5f numb-gli1-2 3 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5f numb-gli1-3 4 8 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role bait bait in vitro translated in vitro translated protein in vitro translated region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction S4c