I2D I2D LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. Oncogene (0950-9232) 2010 imex curation Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-liver Homo sapiens liver liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer Human hepatocellular carcinoma tissue was used for the coimmunoprecipitation. rlzhou@bjmu.edu.cn Accepted 2011-JAN-20 by ORCHARD 06-April-2011: Contacted by JYOTI. Oncogene (0950-9232) 2010 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. Oncogene (0950-9232) 2010 imex curation Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn Accepted 2011-JAN-20 by ORCHARD 06-April-2011: Contacted by JYOTI. Oncogene (0950-9232) 2010 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. Oncogene (0950-9232) 2010 imex curation Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn Accepted 2011-JAN-20 by ORCHARD 06-April-2011: Contacted by JYOTI. Oncogene (0950-9232) 2010 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. Oncogene (0950-9232) 2010 imex curation Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn Accepted 2011-JAN-20 by ORCHARD 06-April-2011: Contacted by JYOTI. Oncogene (0950-9232) 2010 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. LAPTM4B: a novel cancer-associated gene motivates multidrug resistance through efflux and activating PI3K/AKT signaling. Oncogene (0950-9232) 2010 imex curation Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn 06-April-2011: Contacted by JYOTI. orchard Only protein-protein interactions Curated by: I2D - Interologous Interaction Database human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining Li L., Wei XH., Pan YP., Li HC., Yang H., He QH., Pang Y., Shan Y., Xiong FX., Shao GZ., Zhou RL. Cancer - Interactions investigated in the context of cancer rlzhou@bjmu.edu.cn Accepted 2011-JAN-20 by ORCHARD 06-April-2011: Contacted by JYOTI. Oncogene (0950-9232) 2010 orchard Only protein-protein interactions imex curation Curated by: I2D - Interologous Interaction Database mdr1_human ATP-dependent translocase ABCB1 ABCB1 MDR1 PGY1 ATP-binding cassette sub-family B member 1 P-glycoprotein 1 Multidrug resistance protein 1 Phospholipid transporter ABCB1 protein protein human Homo sapiens Human MDLEGDRNGGAKKKNFFKLNNKSEKDKKEKKPTVSVFSMFRYSNWLDKLYMVVGTLAAIIHGAGLPLMMLVFGEMTDIFANAGNLEDLMSNITNRSDINDTGFFMNLEEDMTRYAYYYSGIGAGVLVAAYIQVSFWCLAAGRQIHKIRKQFFHAIMRQEIGWFDVHDVGELNTRLTDDVSKINEGIGDKIGMFFQSMATFFTGFIVGFTRGWKLTLVILAISPVLGLSAAVWAKILSSFTDKELLAYAKAGAVAEEVLAAIRTVIAFGGQKKELERYNKNLEEAKRIGIKKAITANISIGAAFLLIYASYALAFWYGTTLVLSGEYSIGQVLTVFFSVLIGAFSVGQASPSIEAFANARGAAYEIFKIIDNKPSIDSYSKSGHKPDNIKGNLEFRNVHFSYPSRKEVKILKGLNLKVQSGQTVALVGNSGCGKSTTVQLMQRLYDPTEGMVSVDGQDIRTINVRFLREIIGVVSQEPVLFATTIAENIRYGRENVTMDEIEKAVKEANAYDFIMKLPHKFDTLVGERGAQLSGGQKQRIAIARALVRNPKILLLDEATSALDTESEAVVQVALDKARKGRTTIVIAHRLSTVRNADVIAGFDDGVIVEKGNHDELMKEKGIYFKLVTMQTAGNEVELENAADESKSEIDALEMSSNDSRSSLIRKRSTRRSVRGSQAQDRKLSTKEALDESIPPVSFWRIMKLNLTEWPYFVVGVFCAIINGGLQPAFAIIFSKIIGVFTRIDDPETKRQNSNLFSLLFLALGIISFITFFLQGFTFGKAGEILTKRLRYMVFRSMLRQDVSWFDDPKNTTGALTTRLANDAAQVKGAIGSRLAVITQNIANLGTGIIISFIYGWQLTLLLLAIVPIIAIAGVVEMKMLSGQALKDKKELEGSGKIATEAIENFRTVVSLTQEQKFEHMYAQSLQVPYRNSLRKAHIFGITFSFTQAMMYFSYAGCFRFGAYLVAHKLMSFEDVLLVFSAVVFGAMAVGQVSSFAPDYAKAKISAAHIIMIIEKTPLIDSYSTEGLMPNTLEGNVTFGEVVFNYPTRPDIPVLQGLSLEVKKGQTLALVGSSGCGKSTVVQLLERFYDPLAGKVLLDGKEIKRLNVQWLRAHLGIVSQEPILFDCSIAENIAYGDNSRVVSQEEIVRAAKEANIHAFIESLPNKYSTKVGDKGTQLSGGQKQRIAIARALVRQPHILLLDEATSALDTESEKVVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVKEHGTHQQLLAQKGIYFSMVSVQAGTKRQ F0230744AB288C73 p85a_human Phosphatidylinositol 3-kinase regulatory subunit alpha PIK3R1 GRB1 Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha protein protein human Homo sapiens Human MSAEGYQYRALYDYKKEREEDIDLHLGDILTVNKGSLVALGFSDGQEARPEEIGWLNGYNETTGERGDFPGTYVEYIGRKKISPPTPKPRPPRPLPVAPGSSKTEADVEQQALTLPDLAEQFAPPDIAPPLLIKLVEAIEKKGLECSTLYRTQSSSNLAELRQLLDCDTPSVDLEMIDVHVLADAFKRYLLDLPNPVIPAAVYSEMISLAPEVQSSEEYIQLLKKLIRSPSIPHQYWLTLQYLLKHFFKLSQTSSKNLLNARVLSEIFSPMLFRFSAASSDNTENLIKVIEILISTEWNERQPAPALPPKPPKPTTVANNGMNNNMSLQDAEWYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHYRNESLAQYNPKLDVKLLYPVSKYQQDQVVKEDNIEAVGKKLHEYNTQFQEKSREYDRLYEEYTRTSQEIQMKRTAIEAFNETIKIFEEQCQTQERYSKEYIEKFKREGNEKEIQRIMHNYDKLKSRISEIIDSRRRLEEDLKKQAAEYREIDKRMNSIKPDLIQLRKTRDQYLMWLTQKGVRQKKLNEWLGNENTEDQYSLVEDDEDLPHHDEKTWNVGSSNRNKAENLLRGKRDGTFLVRESSKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHYQHTSLVQHNDSLNVTLAYPVYAQQRR DIP protein P27986 original sequence version: 181 DIP protein P27986 original sequence version: 184 DIP protein P27986-1 original sequence version: 135 DIP protein P27986 original sequence version: 135 DIP protein P27986-1 original sequence version: 145 DIP protein P27986-1 original sequence version: 147 DIP protein P27986 original sequence version: 191 B9DAD8416C33140F q86vi4-3 Lysosomal-associated transmembrane protein 4B LAPTM4B Lysosome-associated transmembrane protein 4-beta PSEC0001 protein protein human Homo sapiens Human MTSRTRVTWPSPPRPLPVPAAAAVAFGAKGTDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGLQARRSTLLKTCARARATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA 4E93093E066BE948 lap4b_human Lysosomal-associated transmembrane protein 4B LAPTM4B Lysosome-associated transmembrane protein 4-beta PSEC0001 protein protein human Homo sapiens Human MTSRTRVTWPSPPRPLPVPAAAAVAFGAKGTDPAEARSSRGIEEAGPRAHGRAGREPERRRSRQQRRGGLQARRSTLLKTCARARATAPGAMKMVAPWTRFYSNSCCLCCHVRTGTILLGVWYLIINAVVLLILLSALADPDQYNFSSSELGGDFEFMDDANMCIAIAISLLMILICAMATYGAYKQRAAWIIPFFCYQIFDFALNMLVAITVLIYPNSIQEYIRQLPPNFPYRDDVMSVNPTCLVLIILLFISIILTFKGYLISCVWNCYRYINGRNSSDVLVYVTSNDTTVLLPPYDDATVNGAAKEPPPPYVSA 4E93093E066BE948 laptm4b-35-p-gp 4 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2c laptm4b-35-p-gp-1 5 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2c laptm4b-35-p-gp-2 1 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Fig. 2d laptm4b-35-p-gp-3 1 6 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig. 2d laptm4b-35-pi3k-p85a 3 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q86VI4-3:p.[Pro12_Pro13delinsAlaAla;Pro15Ala] pro12ala-pro13ala-pro15ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain PP AA Thu Jul 07 12:16:56 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was pro12_13_15ala Fri Jul 22 11:12:22 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro12ala,pro13ala,pro15ala' Wed May 02 18:09:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_pro12-13ala_ala,pro15ala' Tue Jun 30 18:26:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro12_Pro13delinsAlaAla;Pro15Ala]' physical association physical association Fig. 3b laptm4b-35-p85a-2 2 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 8 unspecified role unspecified role bait bait Q86VI4-3:p.[Pro12_Pro13delinsAlaAla;Pro15Ala] pro12ala-pro13ala-pro15ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain PP AA Thu Jul 07 12:17:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was pro12_13_15ala Fri Jul 22 11:02:56 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro12ala,pro13ala,pro15ala' Wed May 02 16:52:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_pro12-13ala_ala,pro15ala' Tue Jun 30 19:18:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro12_Pro13delinsAlaAla;Pro15Ala]' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3c