IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. Cancer Cell (1535-6108) 2010 imex curation Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu 13-Sept-2010: Contacted by JYOTI. 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-prostate_carcinoma Homo sapiens prostate carcinoma prostate_carcinoma Prostate carcinoma (cancer) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu Accepted 2010-JUL-16 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. Cancer Cell (1535-6108) 2010 imex curation Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu 13-Sept-2010: Contacted by JYOTI. 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu Accepted 2010-JUL-16 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. Cancer Cell (1535-6108) 2010 imex curation Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu 13-Sept-2010: Contacted by JYOTI. 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu Accepted 2010-JUL-16 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. Cancer Cell (1535-6108) 2010 imex curation Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu 13-Sept-2010: Contacted by JYOTI. 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-vcap Human VCaP prostate cancer cell line vcap VCaP Human prostate cancer cell line http://www.biocompare.com/ProductDetails/1838978/VCaP.html This line was established in 1997 from a vertebral bone metastasis from a patient with hormone refractory prostate cancer. It was passaged as xenografts in mice then cultured in vitro. It is androgen sensitive in vitro and in vivo. Contains the TMPRSS2�ERG gene fusion. ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu Accepted 2010-JUL-16 by ORCHARD. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. Cancer Cell (1535-6108) 2010 imex curation Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu 13-Sept-2010: Contacted by JYOTI. 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu Accepted 2010-JUL-16 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. Cancer Cell (1535-6108) 2010 imex curation Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu 13-Sept-2010: Contacted by JYOTI. 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-vcap Human VCaP prostate cancer cell line vcap VCaP Human prostate cancer cell line http://www.biocompare.com/ProductDetails/1838978/VCaP.html This line was established in 1997 from a vertebral bone metastasis from a patient with hormone refractory prostate cancer. It was passaged as xenografts in mice then cultured in vitro. It is androgen sensitive in vitro and in vivo. Contains the TMPRSS2�ERG gene fusion. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu Accepted 2010-JUL-16 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. An integrated network of androgen receptor, polycomb, and TMPRSS2-ERG gene fusions in prostate cancer progression. Cancer Cell (1535-6108) 2010 imex curation Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu 13-Sept-2010: Contacted by JYOTI. 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-vcap Human VCaP prostate cancer cell line vcap VCaP Human prostate cancer cell line http://www.biocompare.com/ProductDetails/1838978/VCaP.html This line was established in 1997 from a vertebral bone metastasis from a patient with hormone refractory prostate cancer. It was passaged as xenografts in mice then cultured in vitro. It is androgen sensitive in vitro and in vivo. Contains the TMPRSS2�ERG gene fusion. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yu J., Yu J., Mani RS., Cao Q., Brenner CJ., Cao X., Wang X., Wu L., Li J., Hu M., Gong Y., Cheng H., Laxman B., Vellaichamy A., Shankar S., Li Y., Dhanasekaran SM., Morey R., Barrette T., Lonigro RJ., Tomlins SA., Varambally S., Qin ZS., Chinnaiyan AM. Cancer - Interactions investigated in the context of cancer Fig S1C:Cannot add the interaction of H3K4me3 with Housekeeper gene, as antibody is not for any specific Histone. (Nor any interactions from Fig S7C for the same reason.) chIP-seq: direct sequencing of chIP/DNA samples using Illumina Genome Analyser. Source of AR taken from PMID: 15648093 arul@umich.edu Accepted 2010-JUL-16 by ORCHARD. 13-Sept-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions imex curation mmp9_human_gene Human MMP9 gene;Matrix metalloproteinase-9 gene gene human Homo sapiens Human dab2p_human_gene Human DAB2IP gene:Disabled homolog 2-interacting protein gene gene human Homo sapiens Human lat3_human_gene Human SLC43A1 gene gene gene human Homo sapiens Human cadh1_human_gene Human CDH1gene:Epithelial cadherin gene gene human Homo sapiens Human zbt16_human_gene Human ZBTB16 gene;Zinc finger and BTB domain-containing protein 16. gene gene human Homo sapiens Human mmp3_human_gene Human MMP3 gene-Stromelysin-1 gene gene human Homo sapiens Human ndrg1_human_gene Human NDRG1 gene gene gene human Homo sapiens Human urok_human_gene Human Urokinase-type plasminogen activator gene gene gene human Homo sapiens Human andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ DIP protein P10275 original sequence version: 220 DIP protein P10275-1 original sequence version: 213 DIP protein P10275 original sequence version: 216 DIP protein P10275 original sequence version: 143 DIP protein P10275 original sequence version: 174 DIP protein P10275 original sequence version: 169 DIP protein P10275 original sequence version: 188 DIP protein P10275-1 original sequence version: 188 DIP protein P10275 original sequence version: 196 DIP protein P10275 original sequence version: 217 DIP protein P10275-1 original sequence version: 217 DIP protein P10275-1 original sequence version: 207 A73432C55D39AE06 s45a3_human_gene Human SLC45A3 gene:Solute carrier family 45 member 3 gene gene human Homo sapiens Human tpa_human_gene Human Plat gene;Tissue-type plasminogen activator gene gene human Homo sapiens Human erg_human Transcriptional regulator ERG ERG Transforming protein ERG protein protein human Homo sapiens Human MASTIKEALSVVSEDQSLFECAYGTPHLAKTEMTASSSSDYGQTSKMSPRVPQQDWLSQPPARVTIKMECNPSQVNGSRNSPDECSVAKGGKMVGSPDTVGMNYGSYMEEKHMPPPNMTTNERRVIVPADPTLWSTDHVRQWLEWAVKEYGLPDVNILLFQNIDGKELCKMTKDDFQRLTPSYNADILLSHLHYLRETPLPHLTSDDVDKALQNSPRLMHARNTGGAAFIFPNTSVYPEATQRITTRPDLPYEPPRRSAWTGHGHPTPQSKAAQPSPSTVPKTEDQRPQLDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSSNSSCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPPESSLYKYPSDLPYMGSYHAHPQKMNFVAPHPPALPVTSSSFFAAPNPYWNSPTGGIYPNTRLPTSHMPSHLGTYY DIP protein P11308 original sequence version: 138 A5069C393E409483 fam174b_human_gene Human FAM174B gene, LOC400451. gene gene human Homo sapiens Human fkbp5_human_gene Human FKBP5 gene gene gene human Homo sapiens Human cnr1_human_gene Human CNR1gene:Cannabinoid receptor 1 gene gene human Homo sapiens Human c6orf81_human_gene Human C6orf81 gene gene gene human Homo sapiens Human dpca2_human_gene Human C15orf21 gene:Dresden prostate carcinoma protein 2 gene gene human Homo sapiens Human klk3_human_gene Human kallikrein-related peptidase 3 (KLK3) (PSA) gene gene human Homo sapiens Human syua_human_gene Human SNCA gene:Alpha-synuclein gene gene human Homo sapiens Human cux2_human_gene Human CUTL2 gene:Homeobox protein cut-like 2 gene gene human Homo sapiens Human andr_human_gene Human androgen Receptor gene gene human Homo sapiens Human adrb2_human_gene Human ADRB2 gene; Beta-2 adrenergic receptor gene gene human Homo sapiens Human klk2_human_gene Human Kallikrein-2 gene gene gene human Homo sapiens Human p11308-1 Transcriptional regulator ERG ERG Transforming protein ERG ERG-2 protein protein human Homo sapiens Human MIQTVPDPAAHIKEALSVVSEDQSLFECAYGTPHLAKTEMTASSSSDYGQTSKMSPRVPQQDWLSQPPARVTIKMECNPSQVNGSRNSPDECSVAKGGKMVGSPDTVGMNYGSYMEEKHMPPPNMTTNERRVIVPADPTLWSTDHVRQWLEWAVKEYGLPDVNILLFQNIDGKELCKMTKDDFQRLTPSYNADILLSHLHYLRETPLPHLTSDDVDKALQNSPRLMHARNTDLPYEPPRRSAWTGHGHPTPQSKAAQPSPSTVPKTEDQRPQLDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSSNSSCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPPESSLYKYPSDLPYMGSYHAHPQKMNFVAPHPPALPVTSSSFFAAPNPYWNSPTGGIYPNTRLPTSHMPSHLGTYY B29F14B0F5C2C697 ezh2_human _gene Human Histone-lysine N-methyltransferase EZH2 gene gene gene human Homo sapiens Human socs2_human_gene Human SOCS2 gene:Suppressor of cytokine signaling 2 gene gene human Homo sapiens Human tmps2_human_gene Human TMPS2/TMPRSS2 gene gene gene human Homo sapiens Human erg-urok 4 19 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey association association Fig 2A. ChIP-Seq analysis of VCaP cells detected ERG binding to regions around intron one and exon two of the Plau gene. erg-plat 4 18 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 2A. ChIP-Seq analysis of VCaP cells detected ERG binding to the upstream region of the Plat gene. erg-mmp3 4 19 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey association association Fig 2A. ChIP-Seq analysis of VCaP cells detected ERG binding to a downstream region of the MMP3 gene. erg-mmp9 4 8 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 2A. ChIP-Seq analysis of VCaP cells detected ERG binding to a region around exons 10-12 of the MMP9 gene. erg-ndrg1 4 14 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 3D. Representative genes co-occupied by AR and ERG. erg-c6orf81 4 23 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 3D. Representative genes co-occupied by AR and ERG. erg-cutl2 4 27 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 3D, S3B. Representative genes co-occupied by AR and ERG. ar-ndrg1 4 16 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey association association Fig 3D. Representative genes co-occupied by AR and ERG. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-c6orf81-1 4 16 unspecified role unspecified role bait bait 23 unspecified role unspecified role prey prey association association Fig 3D. Representative genes co-occupied by AR and ERG. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-cutl2-1 4 16 unspecified role unspecified role bait bait 27 unspecified role unspecified role prey prey association association Fig 3D, S3B. Representative genes co-occupied by AR and ERG. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-cutl2-1 6 27 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-c6orf81-1 6 19 unspecified role unspecified role bait bait 23 unspecified role unspecified role prey prey association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-ndrg1-1 6 19 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-c6orf81-2 6 23 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-cutl2-2 6 27 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-ndrg1-1 6 14 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-loc400451 6 20 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-loc400451 6 16 unspecified role unspecified role bait bait 20 unspecified role unspecified role prey prey association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-zbt16 6 19 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-zbt16 6 16 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association Fig 3E. ChIP-PCR confirms AR and ERG co-occupancy on selected genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-ar 7 16 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait physical association physical association Fig 3G Coimmunoprecipitation assays demonstrated an endogenous interaction between the AR and ERG proteins; this interaction between AR and ERG was DNA independent. erg-ar-1 2 31 unspecified role unspecified role bait bait halo-erg fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ets domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 16 unspecified role unspecified role prey prey n terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined dna_plus_ligand_bd dna and ligand binding domain sufficient to bind sufficient binding region sufficient to bind inferred by curator inferred by curator modelled by curator modeled by curator range range range range physical association physical association Fig 3H, S3F. AR directly bound to the ETS domain of the ERG protein. This interaction was further pinpointed to the C-terminal half (ETS-3) of the ETS domain. erg-ar-3 4 28 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 4A. AR and ERG co-bind to upstream regions of the AR gene. ar-ar 4 16 unspecified role unspecified role bait bait 28 unspecified role unspecified role prey prey association association Fig 4A. AR and ERG co-bind to upstream regions of the AR gene. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-ar-4 6 28 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 4B. ChIP-PCR confirms ERG binding to an upstream region of the AR gene. In the comment I have used the phrase "upstream region" though the paper states "promoter", as no evidence is provided for the exact genomic location(s) of the promoter(s) and in Fig 4A the paper refers to "cobinding to the regulatory regions" implying there are no other regulatory regions of the gene, which is most likely incorrect.-thus use of the term "promoter" in fig 4B is a little suspect also. erg-klk3 4 19 unspecified role unspecified role bait bait 25 unspecified role unspecified role prey prey association association Fig 5A. AR and ERG cobinding to regulatory regions of representative AR target genes. P11308 decreases expression at RNA level of ENSG00000142515 ar-klk3-3 4 25 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig 5A. AR and ERG cobinding to regulatory regions of representative AR target genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-klk2 4 30 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig 5A. AR and ERG cobinding to regulatory regions of representative AR target genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-klk2 4 19 unspecified role unspecified role bait bait 30 unspecified role unspecified role prey prey association association Fig 5A. AR and ERG cobinding to regulatory regions of representative AR target genes. P11308 decreases expression at RNA level of ENSG00000167751 erg-slc43a1 4 10 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 5A. AR and ERG cobinding to regulatory regions of representative AR target genes. P11308 decreases expression at RNA level of ENSG00000149150 ar-slc43a1 4 10 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig 5A. AR and ERG cobinding to regulatory regions of representative AR target genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-fkbp5 4 21 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig 5A. AR and ERG cobinding to regulatory regions of representative AR target genes. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-fkbp5 4 21 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 5A. AR and ERG cobinding to regulatory regions of representative AR target genes. erg-ezh2 4 32 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 7A. ERG binds to the EZH2 promoter region. Is there any significance to the fact that the ezh2 gene entry is spread over two lines rather than one? erg-ezh2-1 6 32 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 7A. Confirmation that ERG binds to the EZH2 promoter region. ar-tmps2-2 4 34 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. Fig S1C:I cannot find any colour code for the peaks: I assume Pink peaks are LNCaP cell binding, Green is VCaP cells and brown is both. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-tmps2-3 3 34 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-tmps2-4 5 16 unspecified role unspecified role bait bait 34 unspecified role unspecified role prey prey association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. ChIP-PCR was used to confirm AR binding in the androgen-treated vs. control-treated LNCaP cells. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-slc45a3 4 17 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. Fig S1C:I cannot find any colour code for the peaks: I assume Pink peaks are LNCaP cell binding, Green is VCaP cells and brown is both. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-slc45a3-1 3 17 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-slc45a3-2 5 16 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. ChIP-PCR was used to confirm AR binding in the androgen-treated vs. control-treated LNCaP cells. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-c15orf21 4 24 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. Fig S1C:I cannot find any colour code for the peaks: I assume Pink peaks are LNCaP cell binding, Green is VCaP cells and brown is both. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-c15orf21-1 3 24 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-c15orf21-2 5 16 unspecified role unspecified role bait bait 24 unspecified role unspecified role prey prey association association Fig S1C. AR binding to regulatory elements of the androgen-sensitive 5' partners of gene fusions in prostate cancer. ChIP-PCR was used to confirm AR binding in the androgen-treated vs. control-treated LNCaP cells. Fig S1C:I cannot find any colour code for the peaks: I assume Pink peaks are LNCaP cell binding, Green is VCaP cells and brown is both. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-zbt16-1 4 12 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S3B. Representative genes co-occupied by AR and ERG. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-zbt16-1 4 12 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S3B. Representative genes co-occupied by AR and ERG. VCaP cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. ar-klk3-4 5 25 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S5C. ChIP-PCR analysis of LNCaP cells following ERG titration revealed gradually increased ERG binding, but decreased AR binding, on these gene-specific genomic loci. LNCaP cells were infected with increasing amounts of adenovirus expressing ERG. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. erg-klk3-1 5 25 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S5C. ChIP-PCR analysis of LNCaP cells following ERG titration revealed gradually increased ERG binding, but decreased AR binding, on these gene-specific genomic loci. LNCaP cells were infected with increasing amounts of adenovirus expressing ERG. ar-klk2-1 5 16 unspecified role unspecified role bait bait 30 unspecified role unspecified role prey prey association association Fig S5C. ChIP-PCR analysis of LNCaP cells following ERG titration revealed gradually increased ERG binding, but decreased AR binding, on these gene-specific genomic loci. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. LNCaP cells were infected with increasing amounts of adenovirus expressing ERG. erg-klk2-1 5 30 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S5C. ChIP-PCR analysis of LNCaP cells following ERG titration revealed gradually increased ERG binding, but decreased AR binding, on these gene-specific genomic loci. LNCaP cells were infected with increasing amounts of adenovirus expressing ERG. ar-tmps2-5 5 34 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait association association Fig S5C. ChIP-PCR analysis of LNCaP cells following ERG titration revealed gradually increased ERG binding, but decreased AR binding, on these gene-specific genomic loci. Cells were treated with R1881, a synthetic androgen., to increase nuclear binding and activate AR transcription factor activity. LNCaP cells were infected with increasing amounts of adenovirus expressing ERG. erg-tmps2 5 19 unspecified role unspecified role bait bait 34 unspecified role unspecified role prey prey association association Fig S5C. ChIP-PCR analysis of LNCaP cells following ERG titration revealed gradually increased ERG binding, but decreased AR binding, on these gene-specific genomic loci. LNCaP cells were infected with increasing amounts of adenovirus expressing ERG. erg-adrb2 4 29 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6B. ChIP-Seq analysis demonstrated ERG binding to a number of previously reported EZH2 target genes. erg-socs2 4 33 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6B. ChIP-Seq analysis demonstrated ERG binding to a number of previously reported EZH2 target genes. erg-cdh1 4 11 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6B. ChIP-Seq analysis demonstrated ERG binding to a number of previously reported EZH2 target genes. erg-dab2ip 4 9 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6B. ChIP-Seq analysis demonstrated ERG binding to a number of previously reported EZH2 target genes. erg-snca 4 26 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6B. ChIP-Seq analysis demonstrated ERG binding to a number of previously reported EZH2 target genes. erg-adrb2-1 5 29 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6C. ERG recruitment to EZH2 target genes in LNCaP cells with ERG overexpression. 'ERG overexpression' probably refers to LNCaP cell infection with adenovirus expressing ERG, though this is not stated in the figure legend. erg-cdh1-1 5 11 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6C. ERG recruitment to EZH2 target genes in LNCaP cells with ERG overexpression. 'ERG overexpression' probably refers to LNCaP cell infection with adenovirus expressing ERG, though this is not stated in the figure legend. erg-dab2ip-1 5 9 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6C. ERG recruitment to EZH2 target genes in LNCaP cells with ERG overexpression. I think that "ERG overexpression" (in comment above, taken from the paper/figure legend) is referring to adenoviral infection with ERG (as for e.g. Fig S5 C+D), though this is not stated for this figure, and the LNCaP cell line does not overexpress ERG as far as I can find out. (main paper states this cell line is ERG-negative) . 'ERG overexpression' probably refers to LNCaP cell infection with adenovirus expressing ERG, though this is not stated in the figure legend. erg-cnr1 5 19 unspecified role unspecified role bait bait 22 unspecified role unspecified role prey prey association association Fig S6C. ERG recruitment to EZH2 target genes in LNCaP cells with ERG overexpression. I think that "ERG overexpression" is referring to mention of adenoviral infection with ERG, though this is not stated for this figure, and the LNCaP cell line does not overexpress ERG as far as I can find out. (main paper states this cell line is ERG-negative) . 'ERG overexpression' probably refers to LNCaP cell infection with adenovirus expressing ERG, though this is not stated in the figure legend. erg-socs2-1 5 33 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig S6C. ERG recruitment to EZH2 target genes in LNCaP cells with ERG overexpression. I think that "ERG overexpression" is referring to mention of adenoviral infection with ERG, though this is not stated for this figure, and the LNCaP cell line does not overexpress ERG as far as I can find out. (main paper states this cell line is ERG-negative) . 'ERG overexpression' probably refers to LNCaP cell infection with adenovirus expressing ERG, though this is not stated in the figure legend. erg-ar-5 1 16 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait physical association physical association Fig S3E. Coimmunoprecipitation assays demonstrated an endogenous interaction between the AR and ERG proteins; this interaction between AR and ERG was DNA independent.