IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. Cancer Res. (0008-5472) 2010 imex curation Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes Cancer - Interactions investigated in the context of cancer cpark@skku.edu, wkkang@skku.edu 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Only protein-protein interactions human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma confocal microscopy confocal microscopy immunostaining immunostaining Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes cpark@skku.edu, wkkang@skku.edu Accepted 2012-FEB-29 by ORCHARD 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. Cancer Res. (0008-5472) 2010 imex curation Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes Cancer - Interactions investigated in the context of cancer cpark@skku.edu, wkkang@skku.edu 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes cpark@skku.edu, wkkang@skku.edu Accepted 2012-FEB-29 by ORCHARD HeLa cDNA library Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. Cancer Res. (0008-5472) 2010 imex curation Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes Cancer - Interactions investigated in the context of cancer cpark@skku.edu, wkkang@skku.edu 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes BUG FIX: bait= gst-Rad EXP= E.coli. Prey = 35S- GCIP -in vitro. cpark@skku.edu, wkkang@skku.edu Accepted 2012-FEB-29 by ORCHARD 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. Cancer Res. (0008-5472) 2010 imex curation Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes Cancer - Interactions investigated in the context of cancer cpark@skku.edu, wkkang@skku.edu 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunostaining immunostaining Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes cpark@skku.edu, wkkang@skku.edu Accepted 2012-FEB-29 by ORCHARD 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. A novel senescence-evasion mechanism involving Grap2 and Cyclin D interacting protein inactivation by Ras associated with diabetes in cancer cells under doxorubicin treatment. Cancer Res. (0008-5472) 2010 imex curation Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes Cancer - Interactions investigated in the context of cancer cpark@skku.edu, wkkang@skku.edu 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lee I., Yeom S.Y., Lee S.J., Kang W.K., Park C. Diabetes - Interactions investigated in the context of Diabetes cpark@skku.edu, wkkang@skku.edu Accepted 2012-FEB-29 by ORCHARD 19-Jun-2012: Contacted by JYOTI. HeLa cDNA library Cancer Res. (0008-5472) 2010 Only protein-protein interactions imex curation ccdb1_human Cyclin-D1-binding protein 1 Grap2 and cyclin-D-interacting protein Human homolog of Maid CCNDBP1 DIP1 GCIP HHM protein protein human Homo sapiens Human MASATAPAAAVPTLASPLEQLRHLAEELRLLLPRVRVGEAQETTEEFNREMFWRRLNEAAVTVSREATTLTIVFSQLPLPSPQETQKFCEQVHAAIKAFIAVYYLLPKDQGITLRKLVRGATLDIVDGMAQLMEVLSVTPTQSPENNDLISYNSVWVACQQMPQIPRDNKAAALLMLTKNVDFVKDAHEEMEQAVEECDPYSGLLNDTEENNSDNHNHEDDVLGFPSNQDLYWSEDDQELIIPCLALVRASKACLKKIRMLVAENGKKDQVAQLDDIVDISDEISPSVDDLALSIYPPMCHLTVRINSAKLVSVLKKALEITKASHVTPQPEDSWIPLLINAIDHCMNRIKELTQSELEL 40C7C3686632F6A7 rad_human GTP-binding protein RAD RRAD RAD RAD1 Ras associated with diabetes protein protein human Homo sapiens Human MTLNGGGSGAGGSRGGGQERERRRGSTPWGPAPPLHRRSMPVDERDLQAALTPGALTAAAAGTGTQGPRLDWPEDSEDSLSSGGSDSDESVYKVLLLGAPGVGKSALARIFGGVEDGPEAEAAGHTYDRSIVVDGEEASLMVYDIWEQDGGRWLPGHCMAMGDAYVIVYSVTDKGSFEKASELRVQLRRARQTDDVPIILVGNKSDLVRSREVSVDEGRACAVVFDCKFIETSAALHHNVQALFEGVVRQIRLRRDSKEANARRQAGTRRRESLGKKAKRFLGRIVARNSRKMAFRAKSKSCHDLSVL EF098960A371D2C4 rrad-ccndbp1 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 3A rrad-ccndbp1-1 5 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association 3B rrad-ccndbp1-2 3 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 3C rrad-ccndbp1-3 4 7 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3D rrad-ccndbp1-4 1 7 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization S4