IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. pull down pull down western blot western blot Immuno blot Immuno blot Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. confocal microscopy confocal microscopy immunostaining immunostaining Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis BJ/ET/RasV12ER cells were used:-BJ fibroblasts expressing ecotropic receptor, hTERT and 4-OH-tamoxifen (4-OHT)-inducible oncogenic H-RasV12. p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis BJ/ET/RasV12ER cells were used:-BJ fibroblasts expressing ecotropic receptor, hTERT and 4-OH-tamoxifen (4-OHT)-inducible oncogenic H-RasV12. p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis For BRD7 ChIP, formaldehyde crosslinking was preceded by protein–protein crosslinking with 2 mM disuccinimidyl glutarate. BJ/ET/RasV12ER cells were used:-BJ fibroblasts expressing ecotropic receptor, hTERT and 4-OH-tamoxifen (4-OHT)-inducible oncogenic H-RasV12. p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions human-bj Homo sapiens BJ normal foreskin fibroblasts bj Human normal foreskin fibroblasts The ATCC rference number is CRL-2522. The ATCC reference number is CRL-2522. ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis I have NOT used the word "promoter" in the comments (though most likely binding is at the promoter region) as the text does not state this and I cannot read any of the text on the image. BJ/ET/RasV12ER cells were used:-BJ fibroblasts expressing ecotropic receptor, hTERT and 4-OH-tamoxifen (4-OHT)-inducible oncogenic H-RasV12. p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation BRD7 is a candidate tumour suppressor gene required for p53 function. BRD7 is a candidate tumour suppressor gene required for p53 function. Nat. Cell Biol. (1465-7392) 2010 imex curation Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Drost J., Mantovani F., Tocco F., Elkon R., Comel A., Holstege H., Kerkhoven R., Jonkers J., Voorhoeve P.M., Agami R., Del Sal G. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 has been entered as Human based on the stated length of the molecule. mathijs.voorhoeve@duke-nus.edu.sg, r.agami@nki.nl, delsal@lncib.it Accepted 2012-JAN-25 by JYOTI 14-Mar-2012: Contacted by JYOTI. human fetal brain cDNA library Nat. Cell Biol. (1465-7392) 2010 Only protein-protein interactions imex curation ep300_human Histone acetyltransferase p300 Histone butyryltransferase p300 Histone crotonyltransferase p300 Protein propionyltransferase p300 EP300 P300 E1A-associated protein p300 Protein 2-hydroxyisobutyryltransferase p300 Protein lactyltransferas p300 protein protein human Homo sapiens Human MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPDELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVMASQAQQSSPGLGLINSMVKSPMTQAGLTSPNMGMGTSGPNQGPTQSTGMMNSPVNQPAMGMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRQNMQYPNPGMGSAGNLLTEPLQQGSPQMGGQTGLRGPQPLKMGMMNNPNPYGSPYTQNPGQQIGASGLGLQIQTKTVLSNNLSPFAMDKKAVPGGGMPNMGQQPAPQVQQPGLVTPVAQGMGSGAHTADPEKRKLIQQQLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQIISHWKNCTRHDCPVCLPLKNAGDKRNQQPILTGAPVGLGNPSSLGVGQQSAPNLSTVSQIDPSSIERAYAALGLPYQVNQMPTQPQVQAKNQQNQQPGQSPQGMRPMSNMSASPMGVNGGVGVQTPSLLSDSMLHSAINSQNPMMSENASVPSLGPMPTAAQPSTTGIRKQWHEDITQDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAEKIYKIQKELEEKRRTRLQKQNMLPNAAGMVPVSMNPGPNMGQPQPGMTSNGPLPDPSMIRGSVPNQMMPRITPQSGLNQFGQMSMAQPPIVPRQTPPLQHHGQLAQPGALNPPMGYGPRMQQPSNQGQFLPQTQFPSQGMNVTNIPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGSQGSHIHCPQLPQPALHQNSPSPVPSRTPTPHHTPPSIGAQQPPATTIPAPVPTPPAMPPGPQSQALHPPPRQTPTPPTTQLPQQVQPSLPAAPSADQPQQQPRSQQSTAASVPTPTAPLLPPQPATPLSQPAVSIEGQVSNPPSTSSTEVNSQAIAEKQPSQEVKMEAKMEVDQPEPADTQPEDISESKVEDCKMESTETEERSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKIFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAGFVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDLMDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQDRFVYTCNECKHHVETRWHCTVCEDYDLCITCYNTKNHDHKMEKLGLGLDDESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLPSCQKMKRVVQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQQQLQHRLQQAQMLRRRMASMQRTGVVGQQQGLPSPTPATPTTPTGQQPTTPQTPQPTSQPQPTPPNSMPPYLPRTQAAGPVSQGKAAGQVTPPTPPQTAQPPLPGPPPAAVEMAMQIQRAAETQRQMAHVQIFQRPIQHQMPPMTPMAPMGMNPPPMTRGPSGHLEPGMGPTGMQQQPPWSQGGLPQPQQLQSGMPRPAMMSVAQHGQPLNMAPQPGLGQVGISPLKPGTVSQQALQNLLRTLRSPSSPLQQQQVLSILHANPQLLAAFIKQRAAKYANSNPQPIPGQPGMPQGQPGLQPPTMPGQQGVHSNPAMQNMNPMQAGVQRAGLPQQQPQQQLQPPMGGMSPQAQQMNMNHNTMPSQFRDILRRQQMMQQQQQQGAGPGIGPGMANHNQFQQPQGVGYPPQQQQRMQHHMQQMQQGNMGQIGQLPQALGAEAGASLQAYQQRLLQQQMGSPVQPNPMSPQQHMLPNQAQSPHLQGQQIPNSLSNQVRSPQPVPSPRPQSQPPHSSPSPRMQPQPSPHHVSPQTSSPHPGLVAAQANPMEQGHFASPDQNSMLSQLASNPGMANLHGASATDLGLSTDNSDLNSNLSQSTLDIH DIP protein Q09472 original sequence version: 193 DIP protein Q09472 original sequence version: 186 DIP protein Q09472 original sequence version: 107 DIP protein Q09472 original sequence version: 112 DIP protein Q09472 original sequence version: 181 DIP protein Q09472 original sequence version: 183 DIP protein Q09472 original sequence version: 136 DIP protein Q09472 original sequence version: 146 DIP protein Q09472 original sequence version: 165 DIP protein Q09472 original sequence version: 192 DIP protein Q09472 original sequence version: 200 DIP protein Q09472 original sequence version: 206 DIP protein Q09472 original sequence version: 194 DIP protein Q09472 original sequence version: 171 DIP protein Q09472 original sequence version: 142 DIP protein Q09472 original sequence version: 180 8E869E1F174A6FEB brd7_human Bromodomain-containing protein 7 75 kDa bromodomain protein Protein CELTIX-1 BRD7 BP75 CELTIX1 protein protein human Homo sapiens Human MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGEKQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEALNQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIYNKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSGDAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTTLGLLHPVDPIVGEPGYCPVRLGMTTGRLQSGVNTLQGFKEDKRNKVTPVLYLNYGPYSSYAPHYDSTFANISKDDSDLIYSTYGEDSDLPSDFSIHEFLATCQDYPYVMADSLLDVLTKGGHSRTLQEMEMSLPEDEGHTRTLDTAKEMEITEVEPPGRLDSSTQDRLIALKAVTNFGVPVEVFDSEEAEIFQKKLDETTRLLRELQEAQNERLSTRPPPNMICLLGPSYREMHLAEQVTNNLKELAQQVTPGDIVSTYGVRKAMGISIPSPVMENNFVDLTEDTEEPKKTDVAECGPGGS DIP protein Q9NPI1 original sequence version: 69 DIP protein Q9NPI1-1 original sequence version: 82 29B7947644C215E7 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 fas_human_gene Human FAS gene gene gene human Homo sapiens Human bbc3_human_gene Human BBC3 gene gene gene human Homo sapiens Human cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human mdm2_human_gene Human MDM2 gene gene gene human Homo sapiens Human bax_human_gene Human BAX gene gene gene human Homo sapiens Human tp53-brd7 1 13 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey physical association physical association S5 tp53-brd7-2 9 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait c_terminal_region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association S5 brd7-tp53 4 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association Fig 3A 4-OHT (4-hydroxy tamoxifan) induces expression of RasV12, which increased the interaction between the two proteins. brd7-tp53-1 5 12 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey c_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association Fig 3B. 4-OHT (4-hydroxy tamoxifan) brd7-tp53-2 8 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3D BRD7 and BRD7-Delta-BD (a mutant lacking the bromodomain) bound equally well to wild-type p53 and p53-9KR (a mutant in which nine lysines in the carboxy terminus - representing potential acetylation sites, were mutated to arginines). brd7-cdkn1a-1 6 16 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait association association Fig 5A BRD7 bound at the upstream (−2.3 kilobases (kb)) p53-binding site in the P21 promoter. Addition of Etoposide resulted in an increase in chromatin-bound acetylated p53 on all target promoters examined. brd7-mdm2 6 12 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey association association Fig 5A BRD7 bound at thep53-binding region of the HDM2 promoter. Addition of Etoposide resulted in an increase in chromatin-bound acetylated p53 on all target promoters examined. brd7-bbc3 6 15 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait association association Fig 5A, 6F. Less binding of BRD7 was detected on the promoters of the p53 target genes that do not require BRD7 for their transcription, including PUMA, FAS and BAX, although it was higher than that to the promoter of a non-transcribed gene. Addition of Etoposide resulted in an increase in chromatin-bound acetylated p53 on all target promoters examined. brd7-fas 6 12 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey association association Fig 5A, 6F. Less binding of BRD7 was detected on the promoters of the p53 target genes that do not require BRD7 for their transcription, including PUMA, FAS and BAX, although it was higher than that to the promoter of a non-transcribed gene. Addition of Etoposide resulted in an increase in chromatin-bound acetylated p53 on all target promoters examined. brd7-bax 6 12 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey association association Fig 5A Less binding of BRD7 was detected on the promoters of the p53 target genes that do not require BRD7 for their transcription, including PUMA, FAS and BAX, although it was higher than that to the promoter of a non-transcribed gene. brd7-cdkn1a 7 12 unspecified role unspecified role bait bait 16 unspecified role unspecified role unspecified role unspecified role association association Fig 5C BRD7 bound at the upstream (−2.3 kilobases (kb)) p53-binding site in the P21 promoter. brd7-mdm2-1 7 17 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait association association Fig 5C BRD7 showed much broader chromatin binding than p53 at the p21 locus, but a similar profile at the HDM2 locus. tp53-mdm2-3 7 13 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey association association Fig 5C BRD7 showed much broader chromatin binding than p53 at the p21 locus, but a similar profile at the HDM2 locus. tp53-cdkn1a-4 7 13 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey association association Fig 5C BRD7 bound at the upstream (−2.3 kilobases (kb)) p53-binding site in the P21 promoter. ep300-cdkn1a 6 11 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey association association Fig 6A p300 bound to the p21 promoter. brd7-ep300 4 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association Fig 6B tp53-brd7-3 10 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association Fig 6C BRD7 and BRD7-Delta-BD (a mutant lacking the bromodomain) bound equally well to wild-type p53 and p53-9KR (a mutant in which nine lysines in the carboxy terminus - representing potential acetylation sites, were mutated to arginines). brd7-tp53-4 3 12 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization S3C brd7-tp53-5 8 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S11A brd7-ep300-1 2 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association S11B brd7-tp53-6 5 13 unspecified role unspecified role prey prey in vitro In vitro 12 unspecified role unspecified role bait bait n_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 3C. 4-OHT (4-hydroxy tamoxifan)