IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Survival of cancer cells is maintained by EGFR independent of its kinase activity. Survival of cancer cells is maintained by EGFR independent of its kinase activity. Cancer Cell (1535-6108) 2008 imex curation Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. mhung@mdanderson.org,ifidler@mdanderson.org 25-Jun-2008: Contacted by JYOTI. davet Only protein-protein interactions 3 human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. mhung@mdanderson.org,ifidler@mdanderson.org Accepted 2008-JUN-22 by DAVET. Cancer Cell (1535-6108) 2008 davet Only protein-protein interactions 3 imex curation Survival of cancer cells is maintained by EGFR independent of its kinase activity. Survival of cancer cells is maintained by EGFR independent of its kinase activity. Cancer Cell (1535-6108) 2008 imex curation Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. mhung@mdanderson.org,ifidler@mdanderson.org 25-Jun-2008: Contacted by JYOTI. davet Only protein-protein interactions 3 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. The source of EGFR was taken from PMID 2790960, (a paper referred to by the reference Chan and Gill (1996) given by the authors). The ranges of the EGFR ECD and TM domains construct and the ICD construct were taken from the paper referred to by the authors (PMID 17283074; Hsu and Hung, 2007). mhung@mdanderson.org,ifidler@mdanderson.org Accepted 2008-JUN-22 by DAVET. 25-Jun-2008: Contacted by JYOTI. Cancer Cell (1535-6108) 2008 davet Only protein-protein interactions 3 imex curation Survival of cancer cells is maintained by EGFR independent of its kinase activity. Survival of cancer cells is maintained by EGFR independent of its kinase activity. Cancer Cell (1535-6108) 2008 imex curation Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. mhung@mdanderson.org,ifidler@mdanderson.org 25-Jun-2008: Contacted by JYOTI. davet Only protein-protein interactions 3 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Weihua Z., Tsan R., Huang WC., Wu Q., Chiu CH., Fidler IJ., Hung MC. Cancer - Interactions investigated in the context of cancer The PC-3MM2 cell line, a metastatic prostate cancer cell line derived from the parental PC3 line, was used. The source of EGFR was taken from PMID 2790960, (a paper referred to by the reference CHan and Gill (1996) given by the authors). mhung@mdanderson.org,ifidler@mdanderson.org Accepted 2008-JUN-22 by DAVET. 25-Jun-2008: Contacted by JYOTI. Cancer Cell (1535-6108) 2008 davet Only protein-protein interactions 3 imex curation sc5a1_human Sodium/glucose cotransporter 1 SLC5A1 NAGT SGLT1 High affinity sodium-glucose cotransporter Solute carrier family 5 member 1 protein protein human Homo sapiens Human MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPIGASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQRIQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVIMLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFIFGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSECEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEKELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLILGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLCWSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKMKMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA 2B403376595EAB74 egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA DIP protein P00533 original sequence version: 213 DIP protein P00533 original sequence version: 214 DIP protein P00533 original sequence version: 119 DIP protein P00533 original sequence version: 207 DIP protein P00533 original sequence version: 132 DIP protein P00533-1 original sequence version: 197 DIP protein P00533 original sequence version: 163 DIP protein P00533-1 original sequence version: 159 DIP protein P00533 original sequence version: 184 DIP protein P00533-1 original sequence version: 174 DIP protein P00533-1 original sequence version: 191 DIP protein P00533 original sequence version: 218 DIP protein P00533 original sequence version: 221 DIP protein P00533-1 original sequence version: 221 DIP protein P00533 original sequence version: 226 DIP protein P00533 original sequence version: 166 DIP protein P00533-1 original sequence version: 166 DIP protein P00533 original sequence version: 194 DIP protein P00533-1 original sequence version: 194 DIP protein P00533-1 original sequence version: 224 DIP protein P00533 original sequence version: 189 DIP protein P00533-1 original sequence version: 207 DIP protein P00533 original sequence version: 177 DIP protein P00533 original sequence version: 168 D8A2A50B4EFB6ED2 egfr-slc5a1 1 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 5 D we were able to coprecipitate SGLT1 with EGFR This interaction was independent of phosphorylation of EGFR. egfr-slc5a1-1 3 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 5 E immunoprecipitation of EGFR with the C225 antibody coprecipitated SGLT1 with either WT-EGFR or kmtEGFR P13866; P00533 transfection egfr-slc5a1-2 2 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal ECD plus TM required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5 F P13866; P00533 transfection SLC5A1 (SLGT1) coimmunoprecipitates with the EGFR extracellular domain (ECD) plus transmembrane doamin aa 1-648 but not the intracellular domain (ICD) aa 640-1186.