IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Mol. Cell. Biol. (0270-7306) 2008 imex curation Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp Accepted 2008-AUG-4 by JYOTI. A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 jyoti Only protein-protein interactions imex curation Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Mol. Cell. Biol. (0270-7306) 2008 imex curation Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp Accepted 2008-AUG-4 by JYOTI. 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 jyoti Only protein-protein interactions imex curation Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Mol. Cell. Biol. (0270-7306) 2008 imex curation Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. jyoti Only protein-protein interactions mouse-mtd_1a Mus musculus simple epithelial cell line MTD-1A mtd_1a Mouse simple epithelial cell line MTD-1A confocal microscopy confocal microscopy immunostaining immunostaining Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp Accepted 2008-AUG-4 by JYOTI. 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 jyoti Only protein-protein interactions imex curation Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Mol. Cell. Biol. (0270-7306) 2008 imex curation Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. jyoti Only protein-protein interactions mesau-bhk Mesocricetus auratus baby kidney cell line BHK bhk Golden (Syrian) Hamster baby kidney cell line BHK Established from the kidneys of five unsexed, one-day-old hamsters in 1961. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp Accepted 2008-AUG-4 by JYOTI. 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 jyoti Only protein-protein interactions imex curation Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions. Mol. Cell. Biol. (0270-7306) 2008 imex curation Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. jyoti Only protein-protein interactions mouse-mtd_1a Mus musculus simple epithelial cell line MTD-1A mtd_1a Mouse simple epithelial cell line MTD-1A anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nakatsuji H., Nishimura N., Yamamura R., Kanayama HO., Sasaki T. Cancer - Interactions investigated in the context of cancer sasaki@basic.med.tokushima-u.ac.jp Accepted 2008-AUG-4 by JYOTI. 27-Aug-2008: Contacted by JYOTI. A mouse 11-day-old embryo cDNA library was used. Mol. Cell. Biol. (0270-7306) 2008 jyoti Only protein-protein interactions imex curation actn4_mouse Alpha-actinin-4 Non-muscle alpha-actinin 4 Actn4 protein protein mouse Mus musculus Mouse MVDYHAANQAYQYGPNSGGGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKQRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPSLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL 07AA9C92AC228B5A rab13_human Ras-related protein Rab-13 RAB13 GIG4 Cell growth-inhibiting gene 4 protein protein protein human Homo sapiens Human MAKAYDHLFKLLLIGDSGVGKTCLIIRFAEDNFNNTYISTIGIDFKIRTVDIEGKKIKLQVWDTAGQERFKTITTAYYRGAMGIILVYDITDEKSFENIQNWMKSIKENASAGVERLLLGNKCDMEAKRKVQKEQADKLAREHGIRFFETSAKSSMNVDEAFSSLARDILLKSGGRRSGNGNKPPSTDLKTCDKKNTNKCSLG DIP protein P51153 original sequence version: 133 141621CB998178DA milk2_mouse MICAL-like protein 2 Micall2 Jrab Junctional Rab13-binding protein Molecule interacting with CasL-like 2 protein protein mouse Mus musculus Mouse MAAIKALQEWCRQQCEGYRDVSITNMTTSFRDGLAFCAILHRHRPDLINFSALRKENIYENNKLAFQVAEEQLGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMAGIKRPSSDSTEELSGKKGLSQPAKLPSPAQTQRSPLSPARTNPVVQRNEGGSQRPSPKAAPGTAGSSVSSICGVCGKHVHLVQRHLADGRLYHRSCFRCKQCSSTLHSGAYRATGEPGVFVCTHHSSEVTSVSPKSSNLASRKPGGVTADTRPFGVSWTVQEANGEGTPLRVRTAAWEHAGGNTTAKGFVQTELKPPSTSQVHVGSSAGPKLPTITVTTTSVTSKALTHVTNSSPIGWSSPAQSSPANFNSRPVVSPSARNTHLPGSQGQTASKGVKTQLNLNSESSNTAVTPAWTSSASKTQQAREKFFQTPPSAPAPASAPAPAPTSKVPTVVTVPTSKVPNVVTAPTSKVPTVVTVPTSKVPTVVSAPTSKVPTVVSAPTSKVPTVVNSTNSRVTTVVNAPTSKVPTVVSATNGRVPTVVTAHTGRVPAVMNTSASKVSPVVDAPAQESSREQALSVLRKALPALTGSGTQAPNRSFPATSSVLVTLPKNEVPQKVPSDKLSALTTQTPNFTIKLEPSAPVNVGNTAVFLQAGKKSPSISPRVGKTSVGSRPQAEVAGVKGPGPISQEGQEEGPEGWRARLKPVDKKTPAGRSLEQKEPVLAEPRIGDTSRKASSSSDSSVHITLTSIQHKRKPCPAGSGPSPAALSPSPSHRKKLAVPPSLDVSADWLQPEPKKQEDGTRSCKEEKSPTRWSRERSAVLDSGLAPPGEAVTSPVRLHPDYIPQEELQRQLQDIESQLDALELRGVELEKRLRAAEGDASEDSLMVDWFRLIHEKQLLLRLESELMYKSKDQRLEEQQLDLQGELRRLMDKPEGLKSPQDRQREQELLSQYVNTVNDRSDIVDFLDEDRLREQEEDQMLENMIQNLGLQRKKSKSFLSKIWSSKSKSGQA 3D059CD22B8BAF53 actb_mouse Actin, cytoplasmic 1 Actb Beta-actin protein protein mouse Mus musculus Mouse MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 6AFD05CA94E360E2 micall2-actn4 1 6 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region MN sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association BAIT gene AB182579; PREY gene NM_021895 Three independent clones coding for proteins that specifically interacted with JRAB/MICAL-L2-MN were isolated. These positive clones contained sequences identical to actinin-4 cDNA (accession no. NM_021895). micall2-actn4-1 2 8 unspecified role unspecified role bait bait region MN sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association BAIT gene AB182579; PREY gene NM_021895 2 A One of these prey clones encoded amino acids 552 to 912 of actinin-4 and specifically bound to JRAB/MICAL-L2-MN in the same two-hybrid assay micall2-actn4-2 4 8 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association BAIT gene AB182579; PREY gene NM_021895 2 B, 3 B Myc-actinin-4-F was specifically precipitated with HA-JRAB/MICAL-L2-F P57780; Q3TN34 transfection actn4-micall2 4 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association BAIT gene NM_021895; PREY gene AB182579 2 C P57780; Q3TN34 transfection The specific interaction between JRAB/MICAL-L2 and actinin-4 was also detected by reverse coimmunoprecipitation experiments micall2-actn4-3 5 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association BAIT gene AB182579; PREY gene NM_021895 2 D When MTD-1A cell lysates were subjected to immunoprecipitation with anti-JRAB/MICAL-L2 antibody, endogenous actinin-4 was specifically precipitated with JRAB/MICAL-L2 actn4-micall2-1 3 8 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component physical association physical association Q3TN34 gene AB182579; P57780 gene NM_021895 2 E JRAB/MICAL-L2 and actinin-4 primarily colocalized at cell-cell junctions This colocalization took place primarily at cell-cell junctions. micall2-actn4-4 4 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association BAIT gene AB182579; PREY gene NM_021895 3 C P57780; Q3TN34 transfection Although -actin did not directly associate with JRAB/MICAL-L2-F in BHK cells expressing HA-JRAB/MICAL-L2-F alone, the interaction between actin and JRAB/MICAL-L2 was clearly detected when Myc-actinin-4-F was coexpressed in these cells rab13-actn4 4 8 stimulator stimulator prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association PREY gene NM_021895 4 A Q3TN34; P57780; P51153 transfection Myc-actinin-4 was specifically coimmunoprecipitated with HA-Rab13 in the presence of Myc-JRAB/MICAL-L2 This interaction occurred in the presence of Myc-JRAB/MICAL-L2. micall2-actn4-5 4 8 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey P51153:p.Thr22Asn mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T N Wed May 02 20:17:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr22asn' Tue Jun 30 16:29:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr22Asn' P51153:p.Gln67Leu mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 15:52:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln67leu' Tue Jun 30 20:22:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Leu' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association BAIT gene AB182579; PREY gene NM_021895 4 B, D P57780; Q3TN34; P51153 transfection When Myc-actinin-4-F and HA-JRAB/MICAL-L2-F were coexpressed with Myc-Rab13 T22N orMyc-Rab13 Q67L in BHK cells, the amount of Myc-actinin-4-F coimmunoprecipitated with HA-JRAB/MICAL-L2 was increased in the presence of Myc-Rab13 Q67L compared to that of Myc-Rab13 T22N The amount of Myc-actinin-4-F coimmunoprecipitated with HA-JRAB/MICAL-L2 was increased in the presence of Myc-Rab13 Q67L compared to that of Myc-Rab13 T22N. From Fig. 4 B it is not clear if any Rab13 was coimmunoprecipitated when the mutant Rab13 T22N was used. Rab13 T22N is a GTP binding mutant and Rab13 Q67L is a GTP hydrolysis mutant. Th presence of wild-type Rab13 and GTPgammaS increased these interactions in a dose-dependent manner. actn4-micall2-2 4 7 unspecified role unspecified role prey prey P51153:p.Thr22Asn mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T N Wed May 02 18:44:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr22asn' Tue Jun 30 20:22:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr22Asn' P51153:p.Gln67Leu mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 17:58:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln67leu' Tue Jun 30 16:09:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Leu' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association PREY gene AB182579; BAIT gene NM_021895 4 C P57780; Q3TN34; P51153 transfection When Myc-actinin-4-F and HA-JRAB/MICAL-L2-F were coexpressed with Myc-Rab13 T22N orMyc-Rab13 Q67L in BHK cells, the amount of Myc-actinin-4-F coimmunoprecipitated with HA-JRAB/MICAL-L2 was increased in the presence of Myc-Rab13 Q67L compared to that of Myc-Rab13 T22N (Fig. 4B). The effect of Rab13 activation on the actinin-4�JRAB/MICAL-L2 interaction was also detected by reverse coimmunoprecipitation experiments (Fig. 4C). The amount of Myc-actinin-4-F coimmunoprecipitated with HA-JRAB/MICAL-L2 was increased in the presence of Myc-Rab13 Q67L compared to that of Myc-Rab13 T22N. Rab13 T22N is a GTP binding mutant and Rab13 Q67L is a GTP hydrolysis mutant.