IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein kinase C-epsilon protects MCF-7 cells from TNF-mediated cell death by inhibiting Bax translocation. Protein kinase C-epsilon protects MCF-7 cells from TNF-mediated cell death by inhibiting Bax translocation. Apoptosis (1360-8185) 2007 Lu D., Sivaprasad U., Huang J., Shankar E., Morrow S., Basu A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer abasu@hsc.unt.edu 25-Jun-2008: Contacted by JYOTI. davet human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Lu D., Sivaprasad U., Huang J., Shankar E., Morrow S., Basu A. Apoptosis - Interactions involving proteins with a function related to apoptosis abasu@hsc.unt.edu Accepted 2008-JUN-13 by DAVET. Apoptosis (1360-8185) 2007 davet Protein kinase C-epsilon protects MCF-7 cells from TNF-mediated cell death by inhibiting Bax translocation. Protein kinase C-epsilon protects MCF-7 cells from TNF-mediated cell death by inhibiting Bax translocation. Apoptosis (1360-8185) 2007 Lu D., Sivaprasad U., Huang J., Shankar E., Morrow S., Basu A. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer abasu@hsc.unt.edu 25-Jun-2008: Contacted by JYOTI. davet human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lu D., Sivaprasad U., Huang J., Shankar E., Morrow S., Basu A. Apoptosis - Interactions involving proteins with a function related to apoptosis The source of PKC epsilon is taken from PMID 12198125. (Another paper by the authors). abasu@hsc.unt.edu Accepted 2008-JUN-13 by DAVET. 25-Jun-2008: Contacted by JYOTI. Apoptosis (1360-8185) 2007 davet bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG DIP protein Q07812 original sequence version: 73 DIP protein Q07812 original sequence version: 156 DIP protein Q07812 original sequence version: 92 DIP protein Q07812 original sequence version: 94 DIP protein Q07812-1 original sequence version: 93 DIP protein Q07812 original sequence version: 95 DIP protein Q07812 original sequence version: 120 DIP protein Q07812 original sequence version: 126 DIP protein Q07812 original sequence version: 143 DIP protein Q07812 original sequence version: 172 DIP protein Q07812 original sequence version: 175 6C0CDB0A7DEE4994 kpce_human Protein kinase C epsilon type nPKC-epsilon PRKCE PKCE protein protein human Homo sapiens Human MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFVTDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGRVYVIIDLSGSSGEAPKDNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDQVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLIAGAESPQPASGSSPSEEDRSKSAPTSPCDQEIKELENNIRKALSFDNRGEEHRAASSPDGQLMSPGENGEVRQGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVASQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFSYFGEDLMP DIP protein Q02156 original sequence version: 154 DIP protein Q02156 original sequence version: 119 85032D0A091A1F7F bax-bax 1 3 unspecified role unspecified role neutral component neutral component physical association physical association 3, 6 TNF induced formation of Bax dimer and the extent of Bax dimer formation was less in MCF-7/PKCe cells compared to MCF-7/Neo cells. TNF enhances formation of Bax dimers in MCF-7 cells. Overexpression of PKC epsilon reduces formation of Bax dimers. Depletion of PKC espilon with siRNA increased formation of Bax dimers. bax-prkce 2 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 8 an association between PKCe and Bax could be detected in MCF-7/PKCe cells but not in MCF-7/Neo cells and this interaction increased further by TNF treatment. This interaction was enhanced by TNF treatment. Q02156 transfection Q02156 overexpression This interaction only occurred in cells overexpressing PRKCE (PKC epsilon).