IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mdm2 is critically and continuously required to suppress lethal p53 activity in vivo. Mdm2 is critically and continuously required to suppress lethal p53 activity in vivo. Cancer Cell (1535-6108) 2006 imex curation Ringshausen I., O'Shea CC., Finch AJ., Swigart LB., Evan GI. Cancer - Interactions investigated in the context of cancer gevan@cc.ucsf.edu Only protein-protein interactions 2 mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ringshausen I., O'Shea CC., Finch AJ., Swigart LB., Evan GI. Cancer - Interactions investigated in the context of cancer gevan@cc.ucsf.edu Accepted 2007-JAN-15 by JYOTI. Cancer Cell (1535-6108) 2006 Only protein-protein interactions 2 imex curation p53_er_mouse_protein 3' end of mouse p53 fused to modified ligand binding domain of mouse estrogen receptor (binds 4-hydroxy tamoxifen) protein protein mouse Mus musculus Mouse IRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSDNNNRNEMGASGDMRAANLWPSPLVIKHTKKNSPALSLTADQMVSALLDAEPPMIYSEYDPSRPFSEASMMGLLTNLADRELVHMINWAKRVPGFGDLNLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHRRLAQLLLILSHIRHMSNKGMEHLYNMKCKNVVPLYDLLLEMLDAHRLHAPASRMGVPPEEPSQTQLATTSSTSAHSLQTYYIPPEAEGFPNTI An amino acid sequence which is not represented in UniProt The portion of murine p53 coded for by exons 10 and 11 (C-terminal 62 aa) is fused to aa 281-599 of the mouse estrogen receptor alpha (including the modified ligand-binding domain). Three amino acids separate the 2 proteins that are fused. AFE8243A6617AF04 mdm2_mouse E3 ubiquitin-protein ligase Mdm2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein Mdm2 RING-type E3 ubiquitin transferase Mdm2 protein protein mouse Mus musculus Mouse MCNTNMSVSTEGAASTSQIPASEQETLVRPKPLLLKLLKSVGAQNDTYTMKEIIFYIGQYIMTKRLYDEKQQHIVYCSNDLLGDVFGVPSFSVKEHRKIYAMIYRNLVAVSQQDSGTSLSESRRQPEGGSDLKDPLQAPPEEKPSSSDLISRLSTSSRRRSISETEENTDELPGERHRKRRRSLSFDPSLGLCELREMCSGGSSSSSSSSSESTETPSHQDLDDGVSEHSGDCLDQDSVSDQFSVEFEVESLDSEDYSLSDEGHELSDEDDEVYRVTVYQTGESDTDSFEGDPEISLADYWKCTSCNEMNPPLPSHCKRCWTLRENWLPDDKGKDKVEISEKAKLENSAQAEEGLDVPDGKKLTENDAKEPCAEEDSEEKAEQTPLSQESDDYSQPSTSSSIVYSSQESVKELKEETQDKDESVESSFSLNAIEPCVICQGRPKNGCIVHGKTGHLMSCFTCAKKLKKRNKPCPVCRQPIQMIVLTYFN DIP protein P23804 original sequence version: 108 DIP protein P23804 original sequence version: 117 DIP protein P23804 original sequence version: 90 DIP protein P23804 original sequence version: 109 DIP protein P23804 original sequence version: 145 DIP protein P23804 original sequence version: 129 DIP protein P23804 original sequence version: 120 DIP protein P23804 original sequence version: 166 4ABF489A82038DF4 p53_er_mouse-mdm2 1 3 unspecified role unspecified role prey prey 2 unspecified role unspecified role bait bait physical association physical association 1 C we used an antibody specific to Mdm2 to coimmunoprecipitate p53ERTAM from the p53KI/KI MEF lysates used in Figure 1A. p53ERTAM was clearly coimmunoprecipitated along with Mdm2 (Figure 1C). The reverse experiment, using an antibody against p53 (Pab246), gave the converse result. mdm2-p53_er_mouse 1 3 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey physical association physical association 1 C we used an antibody specific to Mdm2 to coimmunoprecipitate p53ERTAM from the p53KI/KI MEF lysates used in Figure 1A. p53ERTAM was clearly coimmunoprecipitated along with Mdm2 (Figure 1C).