IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). Cancer Immun. 2006 Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org 02-MAR-2006: Contacted by JYOTI. A human testis cDNA library was used. orchard human-sk_mel_37 Homo sapiens melanoma derived cell line SK-MEL-37 sk_mel_37 Human melanoma derived SK-MEL-37 cell line fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org Accepted 2007-FEB-7 by ORCHARD. 02-MAR-2006: Contacted by JYOTI. A human testis cDNA library was used. Cancer Immun. 2006 orchard Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). Cancer Immun. 2006 Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org 02-MAR-2006: Contacted by JYOTI. A human testis cDNA library was used. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org Accepted 2007-FEB-7 by ORCHARD. 02-MAR-2006: Contacted by JYOTI. A human testis cDNA library was used. Cancer Immun. 2006 orchard Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). Cancer Immun. 2006 Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org 02-MAR-2006: Contacted by JYOTI. A human testis cDNA library was used. orchard human-sk_mel_37 Homo sapiens melanoma derived cell line SK-MEL-37 sk_mel_37 Human melanoma derived SK-MEL-37 cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org Accepted 2007-FEB-7 by ORCHARD. 02-MAR-2006: Contacted by JYOTI. A human testis cDNA library was used. Cancer Immun. 2006 orchard Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6). Cancer Immun. 2006 Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org 02-MAR-2006: Contacted by JYOTI. A human testis cDNA library was used. orchard human-u266 Homo sapiens myeloma cell line U-266 u266 Human myeloma cell line U-266 confirmed as human with IEF of AST, MDH, NP established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described to produce IgE lambda; cells express mRNA for BCL2 gene anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cho HJ., Caballero OL., Gnjatic S., Andrade VC., Colleoni GW., Vettore AL., Outtz HH., Fortunato S., Altorki N., Ferrera CA., Chua R., Jungbluth AA., Chen YT., Old LJ., Simpson AJ. Cancer - Interactions investigated in the context of cancer asimpson@licr.org Accepted 2007-FEB-7 by ORCHARD. 02-MAR-2006: Contacted by JYOTI. A human testis cDNA library was used. Cancer Immun. 2006 orchard atp5l_human ATP synthase subunit g, mitochondrial ATP5MG ATP synthase membrane subunit g ATP5L protein protein human Homo sapiens Human MAQFVRNLVEKTPALVNAAVTYSKPRLATFWYYAKVELVPPTPAEIPRAIQSLKKIVNSAQTGSFKQLTVKEAVLNGLVATEVLMWFYVGEIIGKRGIIGYDV 03D484BCF836E6C2 magc1_human Melanoma-associated antigen C1 MAGE-C1 antigen Cancer/testis antigen 7.1 MAGEC1 protein protein human Homo sapiens Human MGDKDMPTAGMPSLLQSSSESPQSCPEGEDSQSPLQIPQSSPESDDTLYPLQSPQSRSEGEDSSDPLQRPPEGKDSQSPLQIPQSSPEGDDTQSPLQNSQSSPEGKDSLSPLEISQSPPEGEDVQSPLQNPASSFFSSALLSIFQSSPESTQSPFEGFPQSVLQIPVSAASSSTLVSIFQSSPESTQSPFEGFPQSPLQIPVSRSFSSTLLSIFQSSPERTQSTFEGFAQSPLQIPVSPSSSSTLLSLFQSFSERTQSTFEGFAQSSLQIPVSPSFSSTLVSLFQSSPERTQSTFEGFPQSPLQIPVSSSSSSTLLSLFQSSPERTHSTFEGFPQSLLQIPMTSSFSSTLLSIFQSSPESAQSTFEGFPQSPLQIPGSPSFSSTLLSLFQSSPERTHSTFEGFPQSPLQIPMTSSFSSTLLSILQSSPESAQSAFEGFPQSPLQIPVSSSFSYTLLSLFQSSPERTHSTFEGFPQSPLQIPVSSSSSSSTLLSLFQSSPECTQSTFEGFPQSPLQIPQSPPEGENTHSPLQIVPSLPEWEDSLSPHYFPQSPPQGEDSLSPHYFPQSPPQGEDSLSPHYFPQSPQGEDSLSPHYFPQSPPQGEDSMSPLYFPQSPLQGEEFQSSLQSPVSICSSSTPSSLPQSFPESSQSPPEGPVQSPLHSPQSPPEGMHSQSPLQSPESAPEGEDSLSPLQIPQSPLEGEDSLSSLHFPQSPPEWEDSLSPLHFPQFPPQGEDFQSSLQSPVSICSSSTSLSLPQSFPESPQSPPEGPAQSPLQRPVSSFFSYTLASLLQSSHESPQSPPEGPAQSPLQSPVSSFPSSTSSSLSQSSPVSSFPSSTSSSLSKSSPESPLQSPVISFSSSTSLSPFSEESSSPVDEYTSSSDTLLESDSLTDSESLIESEPLFTYTLDEKVDELARFLLLKYQVKQPITKAEMLTNVISRYTGYFPVIFRKAREFIEILFGISLREVDPDDSYVFVNTLDLTSEGCLSDEQGMSQNRLLILILSIIFIKGTYASEEVIWDVLSGIGVRAGREHFAFGEPRELLTKVWVQEHYLEYREVPNSSPPRYEFLWGPRAHSEVIKRKVVEFLAMLKNTVPITFPSSYKDALKDVEERAQAIIDTTDDSTATESASSSVMSPSFSSE 6C9AE820156F3C50 t2fa_human General transcription factor IIF subunit 1 Transcription initiation factor IIF subunit alpha GTF2F1 RAP74 Transcription initiation factor RAP74 General transcription factor IIF 74 kDa subunit protein protein human Homo sapiens Human MAALGPSSQNVTEYVVRVPKNTTKKYNIMAFNAADKVNFATWNQARLERDLSNKKIYQEEEMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQDEDEEEKEKRGRRKASELRIHDLEDDLEMSSDASDASGEEGGRVPKAKKKAPLAKGGRKKKKKKGSDDEAFEDSDDGDFEGQEVDYMSDGSSSSQEEPESKAKAPQQEEGPKGVDEQSDSSEESEEEKPPEEDKEEEEEKKAPTPQEKKRRKDSSEESDSSEESDIDSEASSALFMAKKKTPPKRERKPSGGSSRGNSRPGTPSAEGGSTSSTLRAAASKLEQGKRVSEMPAAKRLRLDTGPQSLSGKSTPQPPSGKTTPNSGDVQVTEDAVRRYLTRKPMTTKDLLKKFQTKKTGLSSEQTVNVLAQILKRLNPERKMINDKMHFSLKE DIP protein P35269 original sequence version: 109 DIP protein P35269 original sequence version: 171 DIP protein P35269 original sequence version: 164 1032B04A36BDC24F rusc2_human AP-4 complex accessory subunit RUSC2 Interacting protein of Rab1 RUN and SH3 domain-containing protein 2 RUSC2 KIAA0375 protein protein human Homo sapiens Human MDSPPKLTGETLIVHHIPLVHCQVPDRQCCGGAGGGGGSTRPNPFCPPELGITQPDQDLGQADSLLFSSLHSTPGGTARSIDSTKSRSRDGRGPGAPKRHNPFLLQEGVGEPGLGDLYDDSIGDSATQQSFHLHGTGQPNFHLSSFQLPPSGPRVGRPWGTTRSRAGVVEGQEQEPVMTLDTQQCGTSHCCRPELEAETMELDECGGPGGSGSGGGASDTSGFSFDQEWKLSSDESPRNPGCSGSGDQHCRCSSTSSQSEAADQSMGYVSDSSCNSSDGVLVTFSTLYNKMHGTPRANLNSAPQSCSDSSFCSHSDPGAFYLDLQPSPFESKMSYESHHPESGGREGGYGCPHASSPELDANCNSYRPHCEPCPAVADLTACFQSQARLVVATQNYYKLVTCDLSSQSSPSPAGSSITSCSEEHTKISPPPGPGPDPGPSQPSEYYLFQKPEVQPEEQEAVSSSTQAAAAVGPTVLEGQVYTNTSPPNLSTGRQRSRSYDRSLQRSPPVRLGSLERMLSCPVRLSEGPAAMAGPGSPPRRVTSFAELAKGRKKTGGSGSPPLRVSVGDSSQEFSPIQEAQQDRGAPLDEGTCCSHSLPPMPLGPGMDLLGPDPSPPWSTQVCQGPHSSEMPPAGLRATGQGPLAQLMDPGPALPGSPANSHTQRDARARADGGGTESRPVLRYSKEQRPTTLPIQPFVFQHHFPKQLAKARALHSLSQLYSLSGCSRTQQPAPLAAPAAQVSVPAPSGEPQASTPRATGRGARKAGSEPETSRPSPLGSYSPIRSVGPFGPSTDSSASTSCSPPPEQPTATESLPPWSHSCPSAVRPATSQQPQKEDQKILTLTEYRLHGTGSLPPLGSWRSGLSRAESLARGGGEGSMATRPSNANHLSPQALKWREYRRKNPLGPPGLSGSLDRRSQEARLARRNPIFEFPGSLSAASHLNCRLNGQAVKPLPLTCPDFQDPFSLTEKPPAEFCLSPDGSSEAISIDLLQKKGLVKAVNIAVDLIVAHFGTSRDPGVKAKLGNSSVSPNVGHLVLKYLCPAVRAVLEDGLKAFVLDVIIGQRKNMPWSVVEASTQLGPSTKVLHGLYNKVSQFPELTSHTMRFNAFILGLLNIRSLEFWFNHLYNHEDIIQTHYQPWGFLSAAHTVCPGLFEELLLLLQPLALLPFSLDLLFQHRLLQSGQQQRQHKELLRVSQDLLLSAHSTLQLARARGQEGPGDVDRAAQGERVKGVGASEGGEEEEEEEETEEVAEAAGGSGRARWARGGQAGWWYQLMQSSQVYIDGSIEGSRFPRGSSNSSSEKKKGAGGGGPPQAPPPREGVVEGAEACPASEEALGRERGWPFWMGSPPDSVLAELRRSREREGPAASPAENEEGASEPSPGGIKWGHLFGSRKAQREARPTNRLPSDWLSLDKSMFQLVAQTVGSRREPEPKESLQEPHSPALPSSPPCEVQALCHHLATGPGQLSFHKGDILRVLGRAGGDWLRCSRGPDSGLVPLAYVTLTPTPSPTPGSSQN E96E1180A1E18CF8 ctg1b_human Cancer/testis antigen 1 CTAG1A LAGE2A LAGE2B L antigen family member 2 Autoimmunogenic cancer/testis antigen NY-ESO-1 Cancer/testis antigen 6.1 CTAG1B CTAG CTAG1 ESO1 LAGE2 protein protein human Homo sapiens Human MQAEGRGTGGSTGDADGPGGPGIPDGPGGNAGGPGEAGATGGRGPRGAGAARASGPGGGAPRGPHGGAASGLNGCCRCGARGPESRLLEFYLAMPFATPMEAELARRSLAQDAPPLPVPGVLLKEFTVSGNILTIRLTAADHRQLQLSISSCLQQLSLLMWITQCFLPVFLAQPPSGQRR B122C5C2C8BE1569 h2az_human Histone H2A.Z H2AZ1 H2AFZ H2AZ protein protein human Homo sapiens Human MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQKTV DIP protein P0C0S5 original sequence version: 59 DIP protein P0C0S5 original sequence version: 66 DIP protein P0C0S5 original sequence version: 105 E024E53818230371 rm19_human 39S ribosomal protein L19, mitochondrial MRPL15 39S ribosomal protein L15, mitochondrial MRPL19 KIAA0104 Mitochondrial large ribosomal subunit protein bL19m protein protein human Homo sapiens Human MAACIAAGHWAAMGLGRSFQAARTLLPPPASIACRVHAGPVRQQSTGPSEPGAFQPPPKPVIVDKHRPVEPERRFLSPEFIPRRGRTDPLKFQIERKDMLERRKVLHIPEFYVGSILRVTTADPYASGKISQFLGICIQRSGRGLGATFILRNVIEGQGVEICFELYNPRVQEIQVVKLEKRLDDSLLYLRDALPEYSTFDVNMKPVVQEPNQKVPVNELKVKMKPKPWSKRWERPNFNIKGIRFDLCLTEQQMKEAQKWNQPWLEFDMMREYDTSKIEAAIWKEIEASKRS 4056F39694284A9F echd2_human Enoyl-CoA hydratase domain-containing protein 2, mitochondrial ECHDC2 protein protein human Homo sapiens Human MLRVLCLLRPWRPLRARGCASDGAAGGSEIQVRALAGPDQGITEILMNRPSARNALGNVFVSELLETLAQLREDRQVRVLLFRSGVKGVFCAGADLKEREQMSEAEVGVFVQRLRGLMNDIAAFPAPTIAAMDGFALGGGLELALACDLRVAASSAVMGLIETTRGLLPGAGGTQRLPRCLGVALAKELIFTGRRLSGTEAHVLGLVNHAVAQNEEGDAAYQRARALAQEILPQAPIAVRLGKVAIDRGTEVDIASGMAIEGMCYAQNIPTRDRLEGMAAFREKRTPKFVGK 23D76881378D5CDD magec1-ctag1b 2 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Table 1 The MHD of MAGE-C1 (CT7) was used as bait to screen a human testis cDNA library. This study identified NY-ESO-1 (CT6) as a MAGE-C1 binding partner. magec1-rusc2 2 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 8 unspecified role unspecified role prey prey physical association physical association Table 1 Eighteen clones selected from a pool of positive candidates interacted specifically with the MHD upon reconstitution analysis. magec1-gtf2f1 2 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Table 1 Eighteen clones selected from a pool of positive candidates interacted specifically with the MHD upon reconstitution analysis. magec1-echdc2 2 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 12 unspecified role unspecified role prey prey physical association physical association Table 1 Eighteen clones selected from a pool of positive candidates interacted specifically with the MHD upon reconstitution analysis. magec1-atp5l 2 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Table 1 Eighteen clones selected from a pool of positive candidates interacted specifically with the MHD upon reconstitution analysis. magec1-mrpl19 2 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 11 unspecified role unspecified role prey prey physical association physical association Table 1 Eighteen clones selected from a pool of positive candidates interacted specifically with the MHD upon reconstitution analysis. magec1-ctag1b-1 3 6 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 1 A NY-ESO-1 was detected in Western blots by anti-CT7 mAb CT7-33, although with much lower effciency. The band corresponding to NY-ESO-1 is not visible in the picture of the gel. ctag1b-magec1 3 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 1 A Complexes between endogenous MAGE-C1 and NY-ESO-1 proteins were precipitated when both anti-NY-ESO-1 antibodies (NY-41 and E978) were used magec1-ctag1b-3 4 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 1 B MAGE-C1 and NY-ESO-1 were line (data not shown) and U266, a multiple myeloma cell line also co-precipitated when the anti-NY-ESO-1 antibodies were used in two additional cells lines: SK-LC-17, a NSCLC cell ctag1b-magec1-1 4 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 1 B MAGE-C1 and NY-ESO-1 were also co-precipitated when the anti-NY-ESO-1 antibodies were used in two additional cells lines: SK-LC-17, a NSCLC cell line (data not shown) and U266, a multiple myeloma cell line magec1-ctag1b-4 1 9 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 2 MAGE-C1 and NY-ESO-1 appear to co-localize to the cytoplasm of these cells. magec1-h2afz 2 10 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Table 1 Eighteen clones selected from a pool of positive candidates interacted specifically with the MHD upon reconstitution analysis.