IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy. Crystal structure of the hypoxia-inducible form of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3): a possible new target for cancer therapy. J. Biol. Chem. (0021-9258) 2006 Kim SG., Manes NP., El-Maghrabi MR., Lee YH. Cancer - Interactions investigated in the context of cancer His-tagged protein was purified, the tag was removed by thrombin treatment prior to crystallisation. The final purification was performed using Mono Q anion exchange chromatography and the protein maintained under reducing conditions. yhlee@lsu.edu in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kim SG., Manes NP., El-Maghrabi MR., Lee YH. Cancer - Interactions investigated in the context of cancer His-tagged protein was purified, the tag was removed by thrombin treatment prior to crystallisation. The final purification was performed using Mono Q anion exchange chromatography and the protein maintained under reducing conditions. yhlee@lsu.edu Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Biol. Chem. (0021-9258) 2006 f263_human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme iPFK-2 Renal carcinoma antigen NY-REN-56 PFKFB3 protein protein human Homo sapiens Human MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH DIP protein Q16875 original sequence version: 117 A7675A4ADC376879 pfkfb3-pfkfbp3-1 1 2 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction 2.1A Head-to-head functional dimerization. The beta-hairpin N-terminus interacts with the 2-Pase domain to secure binding of fructose-6-phosphate to the active pocket Add PDB xref and r-factors when in PDB and MSD Crystal also contains ATP, EDTA and fructose -6-phosphate Revisit when small molecule editor in place