IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. J. Biol. Chem. (0021-9258) 2006 imex curation Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer BioCreative - Critical Assessment of Information Extraction systems in Biology An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 pull down pull down western blot western blot Immuno blot Immuno blot Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl Accepted 2006-JUL-20 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. J. Biol. Chem. (0021-9258) 2006 imex curation Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer BioCreative - Critical Assessment of Information Extraction systems in Biology An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl Accepted 2006-JUL-20 by ORCHARD. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. J. Biol. Chem. (0021-9258) 2006 imex curation Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer BioCreative - Critical Assessment of Information Extraction systems in Biology An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. pull down pull down western blot western blot Immuno blot Immuno blot Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl Accepted 2006-JUL-20 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. J. Biol. Chem. (0021-9258) 2006 imex curation Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer BioCreative - Critical Assessment of Information Extraction systems in Biology An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl Accepted 2006-JUL-20 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. J. Biol. Chem. (0021-9258) 2006 imex curation Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer BioCreative - Critical Assessment of Information Extraction systems in Biology An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells pull down pull down western blot western blot Immuno blot Immuno blot Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl Accepted 2006-JUL-20 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. J. Biol. Chem. (0021-9258) 2006 imex curation Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer BioCreative - Critical Assessment of Information Extraction systems in Biology An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl Accepted 2006-JUL-20 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. J. Biol. Chem. (0021-9258) 2006 imex curation Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer BioCreative - Critical Assessment of Information Extraction systems in Biology An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions mouse-erythroleukemia Mus musculus me17-1 Murine erythroid cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl Accepted 2006-JUL-20 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. The retinoblastoma family proteins bind to and activate diacylglycerol kinase zeta. J. Biol. Chem. (0021-9258) 2006 imex curation Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer BioCreative - Critical Assessment of Information Extraction systems in Biology An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma pull down pull down western blot western blot Immuno blot Immuno blot Los AP., Vinke FP., de Widt J., Topham MK., van Blitterswijk WJ., Divecha N. Cancer - Interactions investigated in the context of cancer An immunoprecipitation was performed and resulting complexes assayed for diacylglycerol kinase activity by adding lipid vesicles composed of DAG, PI, PIP and PA together with 32P-ATP and cold ATP. The radiolabeled lipid products were separated by TLC and autoradiographed. w.v.blitterswijk@nki.nl Accepted 2006-JUL-20 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation rbl1_human Retinoblastoma-like protein 1 RBL1 pRb1 107 kDa retinoblastoma-associated protein protein protein human Homo sapiens Human MFEDKPHAEGAAVVAAAGEALQALCQELNLDEGSAAEALDDFTAIRGNYSLEGEVTHWLACSLYVACRKSIIPTVGKGIMEGNCVSLTRILRSAKLSLIQFFSKMKKWMDMSNLPQEFRERIERLERNFEVSTVIFKKYEPIFLDIFQNPYEEPPKLPRSRKQRRIPCSVKDLFNFCWTLFVYTKGNFRMIGDDLVNSYHLLLCCLDLIFANAIMCPNRQDLLNPSFKGLPSDFHTADFTASEEPPCIIAVLCELHDGLLVEAKGIKEHYFKPYISKLFDRKILKGECLLDLSSFTDNSKAVNKEYEEYVLTVGDFDERIFLGADAEEEIGTPRKFTRDTPLGKLTAQANVEYNLQQHFEKKRSFAPSTPLTGRRYLREKEAVITPVASATQSVSRLQSIVAGLKNAPSDQLINIFESCVRNPVENIMKILKGIGETFCQHYTQSTDEQPGSHIDFAVNRLKLAEILYYKILETVMVQETRRLHGMDMSVLLEQDIFHRSLMACCLEIVLFAYSSPRTFPWIIEVLNLQPFYFYKVIEVVIRSEEGLSRDMVKHLNSIEEQILESLAWSHDSALWEALQVSANKVPTCEEVIFPNNFETGNGGNVQGHLPLMPMSPLMHPRVKEVRTDSGSLRRDMQPLSPISVHERYSSPTAGSAKRRLFGEDPPKEMLMDKIITEGTKLKIAPSSSITAENVSILPGQTLLTMATAPVTGTTGHKVTIPLHGVANDAGEITLIPLSMNTNQESKVKSPVSLTAHSLIGASPKQTNLTKAQEVHSTGINRPKRTGSLALFYRKVYHLASVRLRDLCLKLDVSNELRRKIWTCFEFTLVHCPDLMKDRHLDQLLLCAFYIMAKVTKEERTFQEIMKSYRNQPQANSHVYRSVLLKSIPREVVAYNKNINDDFEMIDCDLEDATKTPDCSSGPVKEERGDLIKFYNTIYVGRVKSFALKYDLANQDHMMDAPPLSPFPHIKQQPGSPRRISQQHSIYISPHKNGSGLTPRSALLYKFNGSPSKSLKDINNMIRQGEQRTKKRVIAIDSDAESPAKRVCQENDDVLLKRLQDVVSERANH DIP protein P28749 original sequence version: 154 2BC4876EA04BDB31 dgkz-marcks-psd MARCKS-PSD domain of DGK zeta protein protein human Homo sapiens Human YARAAARQARAGKASKKKKRASFKRKSSKK An amino acid sequence which is not represented in UniProt BB720ECE423BB5B9 rbl2_human Retinoblastoma-like protein 2 RBL2 RB2 Retinoblastoma-related protein 2 130 kDa retinoblastoma-associated protein pRb2 protein protein human Homo sapiens Human MPSGGDQSPPPPPPPPAAAASDEEEEDDGEAEDAAPPAESPTPQIQQRFDELCSRLNMDEAARAEAWDSYRSMSESYTLEGNDLHWLACALYVACRKSVPTVSKGTVEGNYVSLTRILKCSEQSLIEFFNKMKKWEDMANLPPHFRERTERLERNFTVSAVIFKKYEPIFQDIFKYPQEEQPRQQRGRKQRRQPCTVSEIFHFCWVLFIYAKGNFPMISDDLVNSYHLLLCALDLVYGNALQCSNRKELVNPNFKGLSEDFHAKDSKPSSDPPCIIEKLCSLHDGLVLEAKGIKEHFWKPYIRKLYEKKLLKGKEENLTGFLEPGNFGESFKAINKAYEEYVLSVGNLDERIFLGEDAEEEIGTLSRCLNAGSGTETAERVQMKNILQQHFDKSKALRISTPLTGVRYIKENSPCVTPVSTATHSLSRLHTMLTGLRNAPSEKLEQILRTCSRDPTQAIANRLKEMFEIYSQHFQPDEDFSNCAKEIASKHFRFAEMLYYKVLESVIEQEQKRLGDMDLSGILEQDAFHRSLLACCLEVVTFSYKPPGNFPFITEIFDVPLYHFYKVIEVFIRAEDGLCREVVKHLNQIEEQILDHLAWKPESPLWEKIRDNENRVPTCEEVMPPQNLERADEICIAGSPLTPRRVTEVRADTGGLGRSITSPTTLYDRYSSPPASTTRRRLFVENDSPSDGGTPGRMPPQPLVNAVPVQNVSGETVSVTPVPGQTLVTMATATVTANNGQTVTIPVQGIANENGGITFFPVQVNVGGQAQAVTGSIQPLSAQALAGSLSSQQVTGTTLQVPGQVAIQQISPGGQQQKQGQSVTSSSNRPRKTSSLSLFFRKVYHLAAVRLRDLCAKLDISDELRKKIWTCFEFSIIQCPELMMDRHLDQLLMCAIYVMAKVTKEDKSFQNIMRCYRTQPQARSQVYRSVLIKGKRKRRNSGSSDSRSHQNSPTELNKDRTSRDSSPVMRSSSTLPVPQPSSAPPTPTRLTGANSDMEEEERGDLIQFYNNIYIKQIKTFAMKYSQANMDAPPLSPYPFVRTGSPRRIQLSQNHPVYISPHKNETMLSPREKIFYYFSNSPSKRLREINSMIRTGETPTKKRGILLEDGSESPAKRICPENHSALLRRLQDVANDRGSH DIP protein Q08999 original sequence version: 144 DIP protein Q08999 original sequence version: 107 D129032FB1F383D4 dgkz_mouse Diacylglycerol kinase zeta Dgkz Diglyceride kinase zeta protein protein mouse Mus musculus Mouse MEPRDPSPEGRSSDSESASASSSGSERDAGPEPDKAPRRLTKRRFPGLRLFGHRKAITKSGLQHLAPPPPTPGAPCGESEEQIQSTVDWSESAVYGEHIWFETNVSGDFCYVGEQHCVAKMLPKSAPRKKCAACKIVVHTQCIKQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMMQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRRSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPREALEMYRKVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLRAEPNPEAGPEERDDGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGMDLTPKIQDLKPQCIVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVLLTTAKAIPVQVDGEPCKLSASRIRIALRNQATMVQKAKRRSTAPLHSDQQPVPEQLRIQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQREPDGAGAKSPMCHQLSSKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPASPCSPTPGSMQGDTALPQGEELIEAAKRNDCCKLQELHRAGGDLMHRDQKSRTLLHHAVSTGSKEVVRYLLDHAPPEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDLQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV AC3E48790E3C3463 rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK DIP protein P06400 original sequence version: 102 DIP protein P06400 original sequence version: 195 DIP protein P06400 original sequence version: 204 DIP protein P06400 original sequence version: 126 DIP protein P06400 original sequence version: 151 DIP protein P06400 original sequence version: 153 DIP protein P06400 original sequence version: 181 DIP protein P06400 original sequence version: 184 DIP protein P06400 original sequence version: 213 DIP protein P06400 original sequence version: 208 DIP protein P06400 original sequence version: 174 DIP protein P06400 original sequence version: 212 DIP protein P06400 original sequence version: 179 DIP protein P06400 original sequence version: 146 C8E746111E19CC32 q13574-2 Diacylglycerol kinase zeta Short DGKZ DAGK6 Diglyceride kinase zeta protein protein human Homo sapiens Human MEPRDGSPEARSSDSESASASSSGSERDAGPEPDKAPRRLNKRRFPGLRLFGHRKAITKSGLQHLAPPPPTPGAPCSESERQIRSTVDWSESATYGEHIWFETNVSGDFCYVGEQYCVARMLKSVSRRKCAACKIVVHTPCIEQLEKINFRCKPSFRESGSRNVREPTFVRHHWVHRRRQDGKCRHCGKGFQQKFTFHSKEIVAISCSWCKQAYHSKVSCFMLQQIEEPCSLGVHAAVVIPPTWILRARRPQNTLKASKKKKRASFKRKSSKKGPEEGRWRPFIIRPTPSPLMKPLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPKEALEMYRKVHNLRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWGGGYTDEPVSKILSHVEEGNVVQLDRWDLHAEPNPEAGPEDRDEGATDRLPLDVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGTAFSDFLMGSSKDLAKHIRVVCDGMDLTPKIQDLKPQCVVFLNIPRYCAGTMPWGHPGEHHDFEPQRHDDGYLEVIGFTMTSLAALQVGGHGERLTQCREVVLTTSKAIPVQVDGEPCKLAASRIRIALRNQATMVQKAKRRSAAPLHSDQQPVPEQLRIQVSRVSMHDYEALHYDKEQLKEASVPLGTVVVPGDSDLELCRAHIERLQQEPDGAGAKSPTCQKLSPKWCFLDATTASRFYRIDRAQEHLNYVTEIAQDEIYILDPELLGASARPDLPTPTSPLPTSPCSPTPRSLQGDAAPPQGEELIEAAKRNDFCKLQELHRAGGDLMHRDEQSRTLLHHAVSTGSKDVVRYLLDHAPPEILDAVEENGETCLHQAAALGQRTICHYIVEAGASLMKTDQQGDTPRQRAEKAQDTELAAYLENRQHYQMIQREDQETAV Major isoform. 7936A979CB3D35AA rb_mouse Retinoblastoma-associated protein Rb1 Rb-1 pRb pp105 p110-RB1 protein protein mouse Mus musculus Mouse MPPKAPRRAAAAEPPPPPPPPPREDDPAQDSGPEELPLARLEFEEIEEPEFIALCQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDNAMSRLLKKYNVLCALYSKLERTCELIYLTQPSSALSTEINSMLVLKISWITFLLAKGEVLQMEDDLVISFQLMLCVVDYFIKFSPPALLREPYKTAAIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFINSLGIVSSNGLPEVESLSKRYEEVYLKNKDLDARLFLDHDKTLQTDPIDSFETERTPRKNNPDEEANVVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFNNCTVNPKENILKRVKDVGHIFKEKFANAVGQGCVDIGVQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTLQHLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDNLEPACPLSLPLQGNHTAADMYLSPLRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLALFYKKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGADEADGSKHLPAESKFQQKLAEMTSTRTRMQKQRMNESKDVSNKEEK DIP protein P13405 original sequence version: 115 DIP protein P13405 original sequence version: 114 DIP protein P13405 original sequence version: 110 AE81D35A07ADB493 rb-dgkz-1 2 14 unspecified role unspecified role prey prey vsv tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 2B These data indicate that DGK is the predominant isotype that binds to pRB. rb-dgkz-2 1 14 unspecified role unspecified role prey prey vsv tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 2B rb-dgkz-3 1 14 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 2C Based on Western blot analysis, we conclude that the C terminus of pRB is the major binding site for DGK. p107-dgkz-1 1 14 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 3A DGK binds to pRB, p107, and p130 in vitro and in cells. p130-dgkz-1 1 14 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 3A DGK binds to pRB, p107, and p130 in vitro and in cells. dgkz-rb-1 6 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3B DGK Binds pRB, p107, and p130 in Cells dgkz-p107-1 6 9 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3C DGK Binds pRB, p107, and p130 in Cells dgkz-p130-1 6 11 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3D DGK Binds pRB, p107, and p130 in Cells dgkz-rb-2 7 12 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association 4A pRB co-immunoprecipitates with endogenous DGK, and vice versa. rb-dgkz-4 7 15 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association 4B pRB co-immunoprecipitates with endogenous DGK, and vice versa. rb-dgkz-5 3 14 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5A DGK associates with pRB via its MARCKS-PSD. dgkz-rb-3 5 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 5B To test whether the DGK-MARCKS-PSD was sufficient to mediate interaction with pRB, we used a biotinylated DGK-MARCKS-PSD peptide to affinity-purify pRB from MCF7 cell lysates. rb-dgkz-6 4 10 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 5D dgkz-rb-4 8 14 unspecified role unspecified role bait bait c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 13 unspecified role unspecified role prey prey physical association physical association 6 DGK binds to hypophosphorylated pRB Phosphorylation of Rb