IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Negative modulation of androgen receptor transcriptional activity by Daxx. Negative modulation of androgen receptor transcriptional activity by Daxx. Mol. Cell. Biol. (0270-7306) 2004 imex curation Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of Daxx and AR inferred from sequence length. shihh@nhri.org.tw Only protein-protein interactions imex curation human-lncap Human caucasian prostate carcinoma lncap Human caucasian prostate adenocarcinoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Cancer - Interactions investigated in the context of cancer Apoptosis - Interactions involving proteins with a function related to apoptosis shihh@nhri.org.tw Mol. Cell. Biol. (0270-7306) 2004 IMEx Negative modulation of androgen receptor transcriptional activity by Daxx. Negative modulation of androgen receptor transcriptional activity by Daxx. Mol. Cell. Biol. (0270-7306) 2004 imex curation Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of Daxx and AR inferred from sequence length. shihh@nhri.org.tw Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer shihh@nhri.org.tw Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Mol. Cell. Biol. (0270-7306) 2004 IMEx Negative modulation of androgen receptor transcriptional activity by Daxx. Negative modulation of androgen receptor transcriptional activity by Daxx. Mol. Cell. Biol. (0270-7306) 2004 imex curation Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of Daxx and AR inferred from sequence length. shihh@nhri.org.tw Only protein-protein interactions imex curation chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Cancer - Interactions investigated in the context of cancer Apoptosis - Interactions involving proteins with a function related to apoptosis shihh@nhri.org.tw Mol. Cell. Biol. (0270-7306) 2004 IMEx Negative modulation of androgen receptor transcriptional activity by Daxx. Negative modulation of androgen receptor transcriptional activity by Daxx. Mol. Cell. Biol. (0270-7306) 2004 imex curation Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of Daxx and AR inferred from sequence length. shihh@nhri.org.tw Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer shihh@nhri.org.tw Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Mol. Cell. Biol. (0270-7306) 2004 IMEx Negative modulation of androgen receptor transcriptional activity by Daxx. Negative modulation of androgen receptor transcriptional activity by Daxx. Mol. Cell. Biol. (0270-7306) 2004 imex curation Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Origin of Daxx and AR inferred from sequence length. shihh@nhri.org.tw Only protein-protein interactions imex curation chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lin DY., Fang HI., Ma AH., Huang YS., Pu YS., Jenster G., Kung HJ., Shih HM. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer shihh@nhri.org.tw Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Mol. Cell. Biol. (0270-7306) 2004 IMEx andr_human Androgen receptor Dihydrotestosterone receptor Nuclear receptor subfamily 3 group C member 4 AR DHTR NR3C4 protein protein human Homo sapiens Human MEVQLGLGRVYPRPPSKTYRGAFQNLFQSVREVIQNPGPRHPEAASAAPPGASLLLLQQQQQQQQQQQQQQQQQQQQQQQETSPRQQQQQQGEDGSPQAHRRGPTGYLVLDEEQQPSQPQSALECHPERGCVPEPGAAVAASKGLPQQLPAPPDEDDSAAPSTLSLLGPTFPGLSSCSADLKDILSEASTMQLLQQQQQEAVSEGSSSGRAREASGAPTSSKDNYLGGTSTISDNAKELCKAVSVSMGLGVEALEHLSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDTAEYSPFKGGYTKGLEGESLGCSGSAAAGSSGTLELPSTLSLYKSGALDEAAAYQSRDYYNFPLALAGPPPPPPPPHPHARIKLENPLDYGSAWAAAAAQCRYGDLASLHGAGAAGPGSGSPSAAASSSWHTLFTAEEGQLYGPCGGGGGGGGGGGGGGGGGGGGGGGEAGAVAPYGYTRPPQGLAGQESDFTAPDVWYPGGMVSRVPYPSPTCVKSEMGPWMDSYSGPYGDMRLETARDHVLPIDYYFPPQKTCLICGDEASGCHYGALTCGSCKVFFKRAAEGKQKYLCASRNDCTIDKFRRKNCPSCRLRKCYEAGMTLGARKLKKLGNLKLQEEGEASSTTSPTEETTQKLTVSHIEGYECQPIFLNVLEAIEPGVVCAGHDNNQPDSFAALLSSLNELGERQLVHVVKWAKALPGFRNLHVDDQMAVIQYSWMGLMVFAMGWRSFTNVNSRMLYFAPDLVFNEYRMHKSRMYSQCVRMRHLSQEFGWLQITPQEFLCMKALLLFSIIPVDGLKNQKFFDELRMNYIKELDRIIACKRKNPTSCSRRFYQLTKLLDSVQPIARELHQFTFDLLIKSHMVSVDFPEMMAEIISVQVPKILSGKVKPIYFHTQ DIP protein P10275 original sequence version: 220 DIP protein P10275-1 original sequence version: 213 DIP protein P10275 original sequence version: 216 DIP protein P10275 original sequence version: 143 DIP protein P10275 original sequence version: 174 DIP protein P10275 original sequence version: 169 DIP protein P10275 original sequence version: 188 DIP protein P10275-1 original sequence version: 188 DIP protein P10275 original sequence version: 196 DIP protein P10275 original sequence version: 217 DIP protein P10275-1 original sequence version: 217 DIP protein P10275-1 original sequence version: 207 A73432C55D39AE06 daxx_human Death domain-associated protein 6 DAXX BING2 DAP6 Daxx Fas death domain-associated protein ETS1-associated protein 1 protein protein human Homo sapiens Human MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD 1B309ADDAA878040 daxx-ar-1 4 6 unspecified role unspecified role prey prey n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain dna binding domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P10275:p.[Lys388Arg;Lys521Arg] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K R certain certain sequence position certain certain certain sequence position certain K R Wed May 02 18:18:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys388arg,lys521arg' Tue Jun 30 18:30:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys388Arg;Lys521Arg]' 7 unspecified role unspecified role bait bait physical association physical association prostate cancer FIG. 5., Fig 7 daxx-ar-3 2 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association prostate cancer FIG. 1D daxx-ar-4 5 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain dna binding domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association prostate cancer Fig. 1A, 5B daxx-ar-5 3 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association prostate cancer revisit when sumoylation in CV 2.5. FIG. 1A. ar-daxx-1 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig. 1B