IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr. Biol. (0960-9822) 2002 imex curation Yoon HJ., Feoktistova A., Wolfe BA., Jennings JL., Link AJ., Gould KL. Cancer - Interactions investigated in the context of cancer Tandem affinity purification was performed and proteins were identified by DALPC mass spectrometry. kathy.gould@mcmail.vanderbilt.edu 29-OCT-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Yoon HJ., Feoktistova A., Wolfe BA., Jennings JL., Link AJ., Gould KL. Cancer - Interactions investigated in the context of cancer Tandem affinity purification was performed and proteins were identified by DALPC mass spectrometry. kathy.gould@mcmail.vanderbilt.edu Accepted 2007-JUL-12 by JYOTI. 29-OCT-2007: Contacted by JYOTI. Curr. Biol. (0960-9822) 2002 Only protein-protein interactions imex curation Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr. Biol. (0960-9822) 2002 imex curation Yoon HJ., Feoktistova A., Wolfe BA., Jennings JL., Link AJ., Gould KL. Cancer - Interactions investigated in the context of cancer Tandem affinity purification was performed and proteins were identified by DALPC mass spectrometry. kathy.gould@mcmail.vanderbilt.edu 29-OCT-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yoon HJ., Feoktistova A., Wolfe BA., Jennings JL., Link AJ., Gould KL. Cancer - Interactions investigated in the context of cancer Tandem affinity purification was performed and proteins were identified by DALPC mass spectrometry. kathy.gould@mcmail.vanderbilt.edu Accepted 2007-JUL-12 by JYOTI. 29-OCT-2007: Contacted by JYOTI. Curr. Biol. (0960-9822) 2002 Only protein-protein interactions imex curation Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr. Biol. (0960-9822) 2002 imex curation Yoon HJ., Feoktistova A., Wolfe BA., Jennings JL., Link AJ., Gould KL. Cancer - Interactions investigated in the context of cancer Tandem affinity purification was performed and proteins were identified by DALPC mass spectrometry. kathy.gould@mcmail.vanderbilt.edu 29-OCT-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Yoon HJ., Feoktistova A., Wolfe BA., Jennings JL., Link AJ., Gould KL. Cancer - Interactions investigated in the context of cancer Tandem affinity purification was performed and proteins were identified by DALPC mass spectrometry. kathy.gould@mcmail.vanderbilt.edu Accepted 2007-JUL-12 by JYOTI. Curr. Biol. (0960-9822) 2002 Only protein-protein interactions imex curation Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr. Biol. (0960-9822) 2002 imex curation Yoon HJ., Feoktistova A., Wolfe BA., Jennings JL., Link AJ., Gould KL. Cancer - Interactions investigated in the context of cancer Tandem affinity purification was performed and proteins were identified by DALPC mass spectrometry. kathy.gould@mcmail.vanderbilt.edu 29-OCT-2007: Contacted by JYOTI. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yoon HJ., Feoktistova A., Wolfe BA., Jennings JL., Link AJ., Gould KL. Cancer - Interactions investigated in the context of cancer Tandem affinity purification was performed and proteins were identified by DALPC mass spectrometry. kathy.gould@mcmail.vanderbilt.edu Accepted 2007-JUL-12 by JYOTI. 29-OCT-2007: Contacted by JYOTI. Curr. Biol. (0960-9822) 2002 Only protein-protein interactions imex curation apc1_yeast Anaphase-promoting complex subunit 1 APC1 N1677 YNL172W protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSKPSTRNDYLPRETHNGEYTGDSPEWQLQINITNKIGGINGDIWLSRDGRSVKWCIEDQCLRQFTYNQKIIKAGYIDFEKTPDCFVVVLSDIAHVYMLKNGGSTTVCFPFQIGNAFWYANGVILERETSASFMDGGYDLKPIEFDLKHKYITLTDPMAPFGLISITNTFKGGINSASGNKTDILQDFQLVLFPSDKDKCIAVFLDRNSKVLRFYYSRILSSDQSRKGELTISSTKKTGLDAAGNSQKSGGISKDLRKFSHLTRRSTSINSNSHDFNAAERVLSGNVGNASGRTDIFALPSSCSRRSLSATLDRMGNNIAPTNRAAPSGFFDSSSANTATHSNITPVSQPMQQQQQEYLNQTATSSKDIVLTEISSLKLPDDIIFTSRRLSSILSKLKFLSLRFERREGLLIFHEPTHFCKIWLIDLLPDVLDSIPFKIYGNSPQNMIRLENLKLKEPSRIQAMYIHELLESCLILVSEGQNKEEYKACLYDPFVKITSPSKNISEELTKQNSLPSLQKLFPYPETSFTKLCFEAVKYITSPAFNISFIFLWQSAYSILLSRANDDVVGGLKMEHDAFSLVLSLLILPIPSSSAQEYQEYKEIYERDLFQHLKQDSEITSSVLPRIVIGLHLIREEYSLNVLCRNEHALLGQFLRFATAAMGWPDLWQSYYVPKMDSESKTFLHPREQNSTFFHPLDEPPSITKSLYSITENSSIPLCPFISFSRLVATDTQVELRITPRSFKILGLYELVHSPNFLPDYVLGILSSFKVDKDELQTYPLGILVPLQNILKILEDKLSEVRDNLELLDRADLQRCSAIINSIRSDSKEVLKRGQRDSYMLCKVPLAKNRSSLSKKPSDIYSILSEIVKSASQVPLDGSAMRMSNIQDDEDIDEGRSLKLNAGLIFSEDKRFTHVVSLLAYYRPTKTQFFTTKTEYAQILAQKKYFAKIMALRTCTNGVGWGAVAYATEKPISTQKWVIQPLNLISVFPDDTKITVKAPEDIAHDIVEWGQFHAGVSSGLRISKKATGITGSWIAFNKPKELDAYHGGFLLGLGLNGHLKNLEEWHIYNYLSPRNTHISIGLLLGMSSSMKGSMDSKLIKVISVHLVAFLPSGSSDLNIDLKLQTAGIIGMGMLYLNSRHKRMSDSIFAQLVSLLNVNDEMVADEEYRLAAGISLGLINLGAGQTKLRKWDSSLLGLGDDLPEDVYDSSDVEQNVMYEDLTTKLLEIVTSTYDVENDWIPENSQIGAVIAIMFLFLKSNNFGISNMLKVDLKEILKANINTRPELLMYREWASNMILWEFIGDDLSFIMKDVDIGVKFSELNTDLLPIYYTMAGRILAMGIRFASTGNLKIRNILLSLVDKFLPLYQYPGKQNLDFRLTISVINVLTNVIVVSLSMVMCASGDLEVLRRVKYLHEVASGPYSDLFQEIPSSKSDVSGVTQVTSNTNTPGNSDRERVDETAASLDDERSSNGSDISDPTAYLEDKKDIDDHYGKFISTNLALGFLFLGSGQYALNTSTLESIAFLSMSVLPTYTTPHPLQELKHFWSMAVEPRCLVIKDISTGDAVNNVPIELVVEEDVEKEEVIREISTPCLLPDFSKIKSIRVKMHGYFPLEVNFTKDYSASDFFSGGTIIYIQRKSESVFENKASFRNVEDIHVALKRKAAESKNYSRLNLKNEQGNTTSSQLVESLGIQDLTMVELDTLLSAGNNTALTDSESYNLGLLCSDKNSGDILDCQLELWYKSFGPHDE 33D32EC47CA40E61 33D32EC47CA40E61 apc13_schpo Anaphase-promoting complex subunit 13 apc13 SPBC28E12.01c SPBC9B6.12c 20S cyclosome/APC complex protein apc13 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDSNYNYVHMNKPGVVLFASDWLKDRLPVDDVEVRVEHLPPVTEDEMTIQHSSANLILMKNKQLRHEPAWKDLELEDLVNAFAFIQGSSNAEGKNTIEDNFETDPFKSVKEAPMAPFLEANRRHQGEHASMRYFR B3896BB1802BF3D8 cdc27_yeast Anaphase-promoting complex subunit CDC27 APC3 CDC27 SNB1 YBL084C YBL0718 Cell division control protein 27 Anaphase-promoting complex subunit 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAVNPELAPFTLSRGIPSFDDQALSTIIQLQDCIQQAIQQLNYSTAEFLAELLYAECSILDKSSVYWSDAVYLYALSLFLNKSYHTAFQISKEFKEYHLGIAYIFGRCALQLSQGVNEAILTLLSIINVFSSNSSNTRINMVLNSNLVHIPDLATLNCLLGNLYMKLDHSKEGAFYHSEALAINPYLWESYEAICKMRATVDLKRVFFDIAGKKSNSHNNNAASSFPSTSLSHFEPRSQPSLYSKTNKNGNNNINNNVNTLFQSSNSPPSTSASSFSSIQHFSRSQQQQANTSIRTCQNKNTQTPKNPAINSKTSSALPNNISMNLVSPSSKQPTISSLAKVYNRNKLLTTPPSKLLNNDRNHQNNNNNNNNNNNNNNNNNNNNNNNNIINKTTFKTPRNLYSSTGRLTTSKKNPRSLIISNSILTSDYSITLPEIMYNFALILRSSSQYNSFKAIRLFESQIPSHIKDTMPWCLVQLGKLHFEIINYDMSLKYFNRLKDLQPARVKDMEIFSTLLWHLHDKVKSSNLANGLMDTMPNKPETWCCIGNLLSLQKDHDAAIKAFEKATQLDPNFAYAYTLQGHEHSSNDSSDSAKTCYRKALACDPQHYNAYYGLGTSAMKLGQYEEALLYFEKARSINPVNVVLICCCGGSLEKLGYKEKALQYYELACHLQPTSSLSKYKMGQLLYSMTRYNVALQTFEELVKLVPDDATAHYLLGQTYRIVGRKKDAIKELTVAMNLDPKGNQVIIDELQKCHMQE A1ECA067AF8784E3 A1ECA067AF8784E3 cdc23_yeast Anaphase-promoting complex subunit CDC23 YHR166C CDC23 Cell division control protein 23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNDDSQDKIIHDIRIQLRKAATELSRWKLYGSSKWAAEALAGLAEAIDVDQTHSLADESPLRNKQGVPKQMFEIPQNGFGLSETEYDLYLLGSTLFDAKEFDRCVFFLKDVTNPYLKFLKLYSKFLSWDKKSQESMENILTTGKFTDEMYRANKDGDGSGNEDINQSGHQRANLKMVSNEHESQSNISSILKEINTFLESYEIKIDDDEADLGLALLYYLRGVILKQEKNISKAMSSFLKSLSCYSFNWSCWLELMDCLQKVDDALLLNNYLYQNFQFKFSENLGSQRTIEFNIMIKFFKLKVFEELNGQLEDYFEDLEFLLQVFPNFTFLKAYNATISYNNLDYVTAESRFDDIVKQDPYRLNDLETYSNILYVMQKNSKLAYLAQFVSQIDRFRPETCCIIANYYSARQEHEKSIMYFRRALTLDKKTTNAWTLMGHEFVELSNSHAAIECYRRAVDICPRDFKAWFGLGQAYALLDMHLYSLYYFQKACTLKPWDRRIWQVLGECYSKTGNKVEAIKCYKRSIKASQTVDQNTSIYYRLAQLYEELEDLQECKKFMMKCVDVEELLEGIVTDETVKARLWLAIFEIKAGNYQLAYDYAMGVSSGTSQEIEEARMLARECRRHM 3C96FE2BC8097118 3C96FE2BC8097118 apc3_schpo Anaphase-promoting complex subunit 3 nuc2 apc3 SPAC17C9.01c SPAC1851.01 20S cyclosome/APC complex protein apc3 Nuclear alteration protein 2 Nuclear scaffold-like protein p76 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTDRLKCLIWYCIDNQNYDNSIFYSERLHAIEDSNESLYLLAYSHFLNLDYNIVYDLLDRVISHVPCTYLFARTSLILGRYKQGISAVEACRSNWRSIQPNINDSISSRGHPDASCMLDVLGTMYKKAGFLKKATDCFVEAVSINPYNFSAFQNLTAIGVPLDANNVFVIPPYLTAMKGFEKSQTNATASVPEPSFLKKSKESSSSSNKFSVSESIANSYSNSSISAFTKWFDRVDASELPGSEKERHQSLKLQSQSQTSKNLLAFNDAQKADSNNRDTSLKSHFVEPRTQALRPGARLTYKLREARSSKRGESTPQSFREEDNNLMELLKLFGKGVYLLAQYKLREALNCFQSLPIEQQNTPFVLAKLGITYFELVDYEKSEEVFQKLRDLSPSRVKDMEVFSTALWHLQKSVPLSYLAHETLETNPYSPESWCILANCFSLQREHSQALKCINRAIQLDPTFEYAYTLQGHEHSANEEYEKSKTSFRKAIRVNVRHYNAWYGLGMVYLKTGRNDQADFHFQRAAEINPNNSVLITCIGMIYERCKDYKKALDFYDRACKLDEKSSLARFKKAKVLILLHDHDKALVELEQLKAIAPDEANVHFLLGKIFKQMRKKNLALKHFTIAWNLDGKATHIIKESIENLDIPEENLLTETGEIYRNLET 97775DC061ECAFBA apc2_yeast Anaphase-promoting complex subunit 2 APC2 RSI1 YLR127C L3105 L3108 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFQITPTRDLKVITDELQTLSSYIFHTNIVDDLNSLLTWMSPNDAKSNHQLRPPSLRIKNIIKVLFPNNATTSPYSMINTSQANNSIVNEGNTNKELQLQLFSTLKEFYIFQVRYHFFLHFNNINYLKDIQRWENYYEFPLRYVPIFDVNVNDWALELNSLRHYLLNRNIKFKNNLRTRLDKLIMDDDFDLADNLIQWLKSANGSLSSTELIVNALYSKINKFCEDNMSRVWNKRFMIMETFNKFINQYWSQFSKLVGCPEDDHELTTTVFNCFESNFLRIRTNEIFDICVLAYPDSKVTLLELRKIMKDFKDYTNIVTTFLSDFKKYILNPSVTTVDALLRYVKTIKAFLVLDPTGRCLHSITTFVKPYFQERKHLVNVLLYAMLDLPEEELKEKINFNVDMKALLSLVDTLHDSDINQDTNITKRDKNKKSPFLWNLKVKGKRELNKDLPIRHAMLYEHILNYYIAWVPEPNDMIPGNIKSSYIKTNLFEVLLDLFESREFFISEFRNLLTDRLFTLKFYTLDEKWTRCLKLIREKIVKFTETSHSNYITNGILGLLETTAPAADADQSNLNSIDVMLWDIKCSEELCRKMHEVAGLDPIIFPKFISLLYWKYNCDTQGSNDLAFHLPIDLERELQKYSDIYSQLKPGRKLQLCKDKGKVEIQLAFKDGRKLVLDVSLEQCSVINQFDSPNDEPICLSLEQLSESLNIAPPRLTHLLDFWIQKGVLLKENGTYSVIEHSEMDFDQAQKTAPMEIENSNYELHNDSEIERKYELTLQRSLPFIEGMLTNLGAMKLHKIHSFLKITVPKDWGYNRITLQQLEGYLNTLADEGRLKYIANGSYEIVKNGHKNS E559A0E28C784DE2 E559A0E28C784DE2 cdc16_yeast Anaphase-promoting complex subunit CDC16 CDC16 YKL022C Cell division control protein 16 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKFCLYCCHCYIVICGKATHYYKSSKATSNLKSSNRVLMRNPMSPSEQHSQHNSTLAASPFVSNVSAARTQQSLPTDAQNDRLQQPWNRTNTATSPYQSLANSPLIQKLQANIMTPHQPSANSNSNSNSITGNVVNDNNLLASMSKNSMFGSTIPSTLRKVSLQREYKDSVDGVVRDEDNDEDVHNNGDAAANANNDRESKLGHNGPLTTTTLTTTTTATQLDVSELSAIERLRLWRFDALMQHMYRTAEYIADKVYNISNDPDDAFWLGQVYYNNNQYVRAVELITRNNLDGVNILCRYLLGLSFVKLQRFDDALDVIGEYNPFSEDPSTTAANTMSNNGNNSNTSQPVTDGGIKMESSLCFLRGKIYFAQNNFNKARDAFREAILVDIKNFEAFEMLLSKNLLTPQEEWDLFDSLDFKEFGEDKEIMKNLYKINLSKYINTEDITKSNEILAKDYKLADNVDVVRSKVDICYTQCKFNECLELCETVLENDEFNTNILPAYIGCLYELSNKNKLFLLSHRLAETFPKSAITWFSVATYYMSLDRISEAQKYYSKSSILDPSFAAAWLGFAHTYALEGEQDQALTAYSTASRFFPGMHLPKLFLGMQFMAMNSLNLAESYFVLAYDICPNDPLVLNEMGVMYFKKNEFVKAKKYLKKALEVVKDLDPSSRTTISIQLNLGHTYRKLNENEIAIKCFRCVLEKNDKNSEIHCSLGYLYLKTKKLQKAIDHLHKSLYLKPNNSSATALLKNALELNVTLSLDASHPLIDKSNLMSQASKDKASLNKKRSSLTYDPVNMAKRLRTQKEIFDQNNKALRKGGHDSKTGSNNADDDFDADMELE A096B34441083488 A096B34441083488 apc11_schpo Anaphase-promoting complex subunit 11 apc11 SPAC343.03 20S cyclosome/APC complex protein apc11 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKVKILRYHAIANWTWDTPKDDVCGICRVPFDGCCPQCTSPGDNCPIVWGKCKHIFHAHCIQNWLATSGSQGQCPMDRQTFVVADSTNEKSETQ C7770021339C3626 mnd2_yeast Anaphase-promoting complex subunit MND2 YIR025W MND2 Meiotic nuclear division protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MARALRDISLFNDIRKDQNSAGAKHERYNMRDLRSKKNQHVNGIDDYEDDSLDRFIRRKKSRVVKYIPSLSAYNVFNEFPYYPTSASQLLDGKLDEFLMLSEQYKSRLPKIRKLGWNRFKPIGINKTMYELEMLRSRARAQNAEGNNEEDFRQHDSREEDPRNNGSIGRVILPHILQENEEYDTGEGVTGLHSMPNDSMAILANNSANNSQNEEVSEEDEISYDYDAEFDHVVDEDDNEEGEVPGEGVEGIEVQRERIVPDDLLMRPTSLSRSLQQFVEEAHHLDRNPYDIDSDNDGEDSKVELDMNPDFEDDVGREHDYNSEYSQEPTSYGGITPDLASNWRNWTRERITSLDELMERRARQQRGQD D41276561E6ED509 D41276561E6ED509 apc15_schpo Anaphase-promoting complex subunit 15 apc15 SPBC83.04 20S cyclosome/APC complex protein apc15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSFMSLMANTAHQLWYPTSFPSMKELEKEEVRLETQELAIKQFGFRTIRPIGLNRSMQEQLDLEEQEREEANQDTELDEEELGSGSFPEEGEMDDMDGDNEEVEDHDIEEVDLDADITNADASEFYEDISEYGFQN 086B97F02A6F0C8B hcn1_schpo Anaphase-promoting complex subunit hcn1 hcn1 SPAC23C11.12 20S cyclosome/APC complex protein hcn1 Chaperone-like protein hcn1 High copy suppressor of cut9 protein 1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLRRNPTAIQITAEDVLAYDEEKLRQTLDSESTTEEALQKNEESTRLSPEKKKIIRERRIGITQIFDSSMHPSQGGAAQS 9402F00563878B29 apc14_schpo Anaphase-promoting complex subunit 14 apc14 omt1 SPAC27D7.05c 20S cyclosome/APC complex protein apc14 Overlapping meiotic transcript protein 1 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDPFAGTGQSKSYRTFGNVFQRLSMSGNPKPLAKKPLLLPEASKAIEFWNYRDVIGGEEAEQERNERASRIAISRIASSRSSIPEYRQAIMQHLTKHVQQLDQLAEW F2DDD34689012489 apc2_schpo Anaphase-promoting complex subunit 2 apc2 SPBP23A10.04 20S cyclosome/APC complex protein apc2 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNDTDLSTFTGRSLLIDQLSSVTQGTPVLDFIDKLRIHFYTTIRQNLLKIDLKNICSLHDLTDQLSDFWLVYEQSVLESPILSPELDRILTCFRCLCRRYLPISVIESVLTEYLDQVLKVWLESKTNPCLDMEKFFQLCEKFKQLGLSSVLKERFVYVLQLHVGSLLTTRYAMSWEQSVYHEALEWIRTEFGVLVEHVFSLSNPAVLVQLDHLVSQILAHLRSDNILDIVLHYPNSLGAIEDLRLVARQKQQRQYLTETFVKDCTSSILTASSDSSYILLFYVSTIRCFVALDPPGVLLDKAAKPIRSFLNEREDAYKCLVSLLFVDGEKGLRSELSQIPTENIDSTTDRFDNYHWMPDPIDAAPDFKKPTDRDVVGSLISIFKSKEPLVKELQLLLADRLLQLTDYHYEVEAKNIEFLKYRFGETVLQMCSVMLNDIENSRFIDQSIHMENYVSKGLHVTILSRLFWPTLSVRYFHLPGPLKKELDAYAEEYRERKRKRELVFLPNLGSVELEIELEDRTLTLTVTPEQAAFISLFEETSTLHIEKAAELLDQPKEIVERHLKFWLHHRVLTDIGDDRYRVRETEAETATETVLDEIQGVSAVQSEAESSAAEMRVYWSFVVGMLTNLGALELERIHNMLTMFIPPPNGYTRTQSELREFLALMIKEEKLEFTGGAYKLK 891764869C542EEB apc9_yeast Anaphase-promoting complex subunit 9 APC9 YLR102C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNQNGDKNEGKLFQLPSLPPWKTPRFNKANFNNFTTPLRKRSTRVINDDSMPITGEVLEERTADDLYGINMDVDEVDYLNTLSHIEEEKQYDYSPFCERNTLRESRIDSFLKAERAAHCLVFHKVGHLDGIDSYRPDIDIMCGEEANKYDSANPEGNGSMLLESVPGCNKEDLERLSRREFVTNSKPNMRRLDDIINHETNALKSFWNDSGLVNSLQSHHLHEEYLLLQEELKNVYKIKCHDRVPIESLRDKCRRHYSNEDSSFL 63423CC7CDFF47B2 63423CC7CDFF47B2 apc8_schpo Anaphase-promoting complex subunit 8 cut23 apc8 SPAC6F12.14 20S cyclosome/APC complex protein apc8 Cell untimely torn protein 23 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTVSLNTMMGVDGLEDQEQLREIRNCLLKCISECSERGLVYAVRWAAEMLNGMNPIEMEHIPFSSTPTGEFDLDPDMANEKLLEVEEKNIYLLAKSYFDCKEFERAAYTLQNCKSSKSIFLRLYSKYLAGEKKSEEENETLLNTNLTLSSTNREFYYISEVLESLHYQGNKDPYLLYLSGVVYRKRKQDSKAIDFLKSCVLKAPFFWSAWLELSLSIDSLETLTTVVSQLPSTHIMTKIFYVYASHELHQVNSSAYEKLAEAEIIFPNSRYLKTQRALLTYDSRDFDEAESLFENILTNDPYRLDDMDTYSNVLFVLENKSKLGFLAQVASSIDKFRPETCSIIGNYYSLLSEHEKAVTYFKRALQLNRNYLSAWTLMGHEYVELKNTHAAIESYRLAVDVNRKDYRAWYGLGQTYEVLDMHFYALYYFQRATALRPYDQRMWQALGNCYEKIDRPQEAIKSYKRALLGSQTNSSILVRLGNLYEELQDLNSAASMYKQCIKTEETEISPETIKARIWLARWELGKKNYREAELYLSEVLNGDLELEEAKALLRELRSRMEHSYD 02B4C9CC07D21BB0 cut9_schpo Anaphase-promoting complex subunit cut9 Cell untimely torn protein 9 cut9 dre1 SPAC6F12.15c 20S cyclosome/APC complex protein cut9 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVVKRTQTDSRMQSTPGNHNHPDAHANAAYMTPPSMGALNANNSNSQLSTLTISPMTYLANNTSTDGSFLKERNAQNTDSLSREDYLRLWRHDALMQQQYKCAAFVGEKVLDITGNPNDAFWLAQVYCCTGDYARAKCLLTKEDLYNRSSACRYLAAFCLVKLYDWQGALNLLGETNPFRKDEKNANKLLMQDGGIKLEASMCYLRGQVYTNLSNFDRAKECYKEALMVDAKCYEAFDQLVSNHLLTADEEWDLVLKLNYSTYSKEDAAFLRSLYMLKLNKTSHEDELRRAEDYLSSINGLEKSSDLLLCKADTLFVRSRFIDVLAITTKILEIDPYNLDVYPLHLASLHESGEKNKLYLISNDLVDRHPEKAVTWLAVGIYYLCVNKISEARRYFSKSSTMDPQFGPAWIGFAHSFAIEGEHDQAISAYTTAARLFQGTHLPYLFLGMQHMQLGNILLANEYLQSSYALFQYDPLLLNELGVVAFNKSDMQTAINHFQNALLLVKKTQSNEKPWAATWANLGHAYRKLKMYDAAIDALNQGLLLSTNDANVHTAIALVYLHKKIPGLAITHLHESLAISPNEIMASDLLKRALEENSLTSGFLNSKYVFEDEVSEYMQQSNLNTSDKSMSMEDQSGKVTESVNDRTQVLYADSRSEMMMDDIEGNVSEQR 5300382396270BE2 apc11_yeast Anaphase-promoting complex subunit 11 D2900 YDL008W APC11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVKINEVHSVFAWSWHIPSTSDEDAANNDPIGNDEDEDVCGICRASYNGTCPSCKFPGDQCPLVIGLCHHNFHDHCIYRWLDTPTSKGLCPMCRQTFQLQKGLAINDAHVQKFVEIVSRRREEMIEEGVAEEFVDFDEPIRQNTDNPIGRQQVDTILDEDFLLR 99F8E8C6BC841934 99F8E8C6BC841934 apc10_yeast Anaphase-promoting complex subunit DOC1 Destruction of cyclin B protein 1 DOC1 APC10 YGL240W HRC283 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDPIGINKVLDHLAPSELIKPVKSCHNKPSVLVLDDRIVDAATKDLYVNGFQEEIQYQNPTPENLQHMFHQGIEILDSARMINVTHLALWKPSSFKLGNPVDFALDDNYDTFWQSDGGQPHQLDIMFSKRMDICVMAIFFSMIADESYAPSLVKVYAGHSPSDARFYKMLEVRNVNGWVALRFLDNREDDQLLKCQFIRLLFPVNHENGKDTHLRGIRLYVPSNEPHQDTHEWAQTLPETNNVFQDAILR F3C58B1BB986442D F3C58B1BB986442D apc5_yeast Anaphase-promoting complex subunit 5 APC5 RMC1 YOR249C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKYGPLGITNFITPYDLCILILIHAHCSQDNGISVPTAVFLRLISPTRPSLEWNPLLKDNSNLRSSSIVPPPVLPILDNIIRILLDDKDGNKIALTLMGYLEAINGLDSINRLMMDLEKNCLVNNYRSMKMRTTSTRRQMTRASFLGTFLSTCIRKYQIGDFEMRETIWINLQNFKTVFKHTPLWLRFKDNVHIQKVKNCLLANDEISVEDQQMVEFFQHFNNGNDADSKTMNEENYGTLISIQHLQSIVNRQIVNWLDNTEFNLMGQEETSSTYEEQSGLVFDLLDTLSLNDATKFPLIFILKYLEAIKENSYQTALDSLHNYFDYKSTGNSQNYFHISLLSLATFHSSFNECDAAINSFEEATRIARENKDMETLNLIMIWIINFIEVHPEYANRFYITVEQIIKYLKNSSDVEDANIFSNAYKFETLLSMVKESKTAEVSSSLLKFMAITLQNVPSQNFDLFQSLVSYEVKFWKELGYESISDVYEKFLSKTSSSSLRNYDSSIINQDIKVAFKALEEDDFLKVKQYLLKSESLELDYDQKINLKYLRVKYLVKIGDYDLSMRLINQYVKECCEEVADSNWRFKFEIESINVLLLSDVGIRSLPKIIKLIDEYKEIGNPLRCVILLLKLCEVLIQVGKSMEAECLISCNLSTILEFPFVRKKTDELLESLSVEEDRDVQMT 59310B47D5A1865F 59310B47D5A1865F cdc26_yeast Anaphase-promoting complex subunit CDC26 YFR036W CDC26 HIT3 SCD26 Cell division control protein 26 protein protein yeast Saccharomyces cerevisiae Baker's yeast MIRRAPTTLQLSHDDVTSLIDDLNEQKLKQQLNIEKTKYFQGKNGGSLHSNTDFQDTSQNIEDNNNDNDNDIDEDDDMSSYNDKAASVAHTRVLNSLHLSTDSNTAHETSNANDNHNPFYIREE F41EECD2B56C4FB5 F41EECD2B56C4FB5 apc1_schpo Anaphase-promoting complex subunit 1 cut4 apc1 SPBC106.09 20S cyclosome/APC complex protein apc1 Cell untimely torn protein 4 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLELKTNVIQDPELKDSLDLQENDRVEIIGDAVHYIVNDHLNRVFNYTVDQQKIHAALITTFASGKKAIVVILDDIGYVYYVGDNNNDSYIINVPFSTESAWSSPSGLYLQRHRSSDENLNTDLPHIFCLNDPLDELTLIKFDGKKILSLFDSIVYVVGEIVVTHNKKEKKLSFWRSRFIDPESDQSIKSSRNRRRESSFSREKNPDLTRSDSIHYTANTRLSEKFEEQGLAYSTIFSHIESFPITGSTSFDSILGNGVLVITTLVKELEKGYMMLFRLVRDRSPYFLDSLQLHAINLSAIKSKHLQKIVVLSSKGKVSLESPMSPSLPIEGTFRSFRVHGATLYLEDTDGVQRYISLDNRASNSLVKWCLSVIRYVLPLREYEIFYTGHLYALFAFKLSHDEAFISSILACFTFFSRDKVHVEPIEDCNEAYSLSSKFHFKKEILIASQLSSHLDYSTFKNYLMPLAITLHFISEELRLDSVVKPRKDQLVALLLQITTWLKWPRYCEYYNFDIAETFLSIPLSIQVDVEEPVGPTSILQWIIECLRSQSTVPFYGLESYGLPHSCSTMFPQTLSLMQLLDCLLNPNMTLQNLVEEMVRLGISRKRCERYPFGILCIIFTVLEIAAEEYSPNWESEELRLVNRLDVDSFLHPKTPKWVFNKQDQEVKEIKALTSTVTDSTLVDTQSFHPYKVVTDMIFREDRRLAEVNKLLNYSSQITIMTEHFDVDLSSVPMQQKVAQCICVRTLSVPIGAGMLTYGSKNPLPTEKVTPRLFNFTLHLHPGTLIIQPNKEFVTQELTEWPEFNVGVALGLSISKFSKEINTSWIMFNRPETLTAYHAGFLFGLGLNGHLKALATWHSFIYLTSKHDTTSIGLLLGLASSYLGSMDAKVTKLLSVHISALLPVGSNELNISPLTQTAGILGIGLLFHDSCHRRMSEVTMEEILASNESELKNEGYKLAAGFSLGLINLGRGSNLPGMSDLKLVSRLQVGISSQATFQSLEAGSPGAIMALTMIYMKTNDLEVAKKIDIPKSRYLLDFYRPDLILLRVAGKNLIMWDEVKADYEWVKYQIPDIMLSQFDLQEKKVLSSDDLLLYNVLAGICFSLGLRFAGTGNPKAKEILINFLDSFIRLCHLPAKTHDERVTAVTVIRCTQIVALSSSCVMAGYCDLDVLRRLRVLHGRMEPVNYGAQMATHMALGILSLGGGRYSLSRSNLAIAALLISFYPQFPRTTQDNRAHLQAARNLWALAVEERCIIPRNQDTKQPCIVPLNVVQKSGAVQKLEAPILLPPYDSISSVSTLGDKYWNLKIDLDNNSDYRELLRESQTLTLMPYDRTSSKEEPLNLFPKLKDTSSPLWNLVKTSRLFQSSNSPLNVASLQESNNKTSLGVKLLLSMDFDNLTRDRLLSLQILLQFFESCWTGVLLNKFHSRQYLFLSRDLVEDLSLRVWEYVYSHNHNEESV 182E65BDA3A82183 apc4_schpo Anaphase-promoting complex subunit 4 cut20 apc4 lid1 SPAC19G12.01c SPAPJ698.04c 20S cyclosome/APC complex protein apc4 Cell untimely torn protein 20 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVSKSFKYPKKHKFEWINETNRVERVGSGLKRVILCPSMELIAILTCSNHLICCRSNSQRIWDVDFHDLEATELCWNHDGNLIVVGFKNGELKIIDSSTGHLVEQRPASRDLAVLMITWAMQETIVNEKRNDFLFDATAYMPLLGTLPSSAKEERIFSSKAIAQFFEPRKREGENNKKVELLSILDERGIRYINMFSSYKIGESDSLKSALNLGVPISHSITNDLAYHVLICKGGTNISLKTLYMPLLKNDLGSIVDIATMSTRMQHLVRYLEEVLNAMYEEFDNVFKSEASFIKTFDALVSKYSDTTFFSLQLELYQFIMNGIPSDLLKEWINERVGDRVLKNWERAMVNSYTSLIIFCQEFVIPACERLTVLLSSARGKSIWGHMKGNTLLDAKLVEDCLATLGYLQNNVFSFLNCLFEEKKYMKHFISWLNYAIVEFNTSEPSSIPPQEIIEHINETVIYIRHSLFRSKLTSYFMGTKPLQLRDPDYYSLKDFANQDDSNSVDDFVSFKTLKESLRDSFNVIFSYPSLTCQKQWLKTGDLVLFEGTDWNVSSLIPKSCNEKNQLFSLFFRKDTPNIFLIISQLMENTMLPVSGCHFGLDYAELLGSSLLDFQPATVLDMKLLNGSSILILGKLKEKCFLCEICLADVPLTFFEHQQKNSYLDAISHLPFIPLNSCLWLHEFEKDFLPSTLEYALSENSDYGVLISRESSRYRLFSF 714A9964499991B8 swm1_yeast Anaphase-promoting complex subunit SWM1 Spore wall maturation protein 1 Anaphase-promoting complex subunit 13 APC13 SWM1 YDR260C YD9320A.11 YD9320A.11c protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSSYRDSYFQYRHLPAPHHILYAEWNQDILALPDEVANITMAMKDNTRTDAEEGRAPQDGERNSNVRESAQGKALMTSEQNSNRYWNSFHDEDDWNLFNGMELESNGVVTFAGQAFDHSLNGGTNSRNDGANEPRKETITGSIFDRRITQLAYARNNGWHELALPQSR 7DD5FC4CBDE8BF19 7DD5FC4CBDE8BF19 apc4_yeast Anaphase-promoting complex subunit 4 APC4 YDR118W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSPINDYFIDYNPLFPIFATRIAKGLAIYRVSDHARLAVIPIRNINLVANYDWDTTTGKFLSIFFKDGTIRIHDIFKDGRLVSFLRIPSTKISKGIWDRIPLRYEPNNRDFACNIIDDLPKLIRFVKDSKRINIVPYTQPNSLWRGPDEDDLDSNEKLDVHVVFNEGNDKITVFFNGDYAVFLSVDNIENENSLKSIIKVQDGFYQCFYEDGTVQTLNLGPLLQSKSSVNLLNYIMVIKELIGYMLTHLEFINRELATPYLDFVKRLCDEAYGYGKLKSELEALFLLGEISCDLEDWLCNSVGEKNFKRWKYLGCEAYQKTVQILTLIFVPACERIIIYVEKLRAILQAFSIQNKLSYTSDLTAVEVLLKSSQKLLTMTLNSIIGLGRDETLFEKFFIWFNDRLHEALDEDYKLKFQFEDDLYFGYDLLSYFDRILSKKGTEPSSIIDVKLYRDLINSMSDMEKDIAQSNVNSHIQQHILVDLKTDVFAQKYPSSQINLLDAIKLPKHNYIVYLIQVTKHNSAQEPFSEENKKKLYIGTLKDENLGIISKESSVKIPALFKSYRLSSTRFVPNRVHSLLRDIGLSDSNYHSSHVTDYRGENYENEEDDGTIAIPAYIRENRENDDFIACTAKVSVDGRSASLVFPKEKQNV CC7E3818FFD6AF25 CC7E3818FFD6AF25 apc10_schpo Anaphase-promoting complex subunit 10 apc10 SPBC1A4.01 SPBC1E8.06 20S cyclosome/APC complex protein apc10 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MAQIRQEALKKQKSETQKSTEGFVDIGNLAQWTCSSEKSGFPIRLVRDDNIDTYWQSDGSQPHTIHIKFVKRVSIKYVSMYLQYTLDESYTPSTLRISAGTGFQDLEIVTTVQVEEPTGWVHVPVGDFGRNGLLDVHLIQIKILANHQSGKDSHVRLIKIYAPEIEQPAIAVDEIPYTSLQFISRNQLR 91AB73EA9D2FDE05 apc5_schpo Anaphase-promoting complex subunit 5 apc5 SPAC959.09c SPAP32A8.01c 20S cyclosome/APC complex protein apc5 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MQSPAMSMVTPIFPIPLKTNSSKKNFYTTFLTPHKLTLCLLIELYAHRIIQPQHCVPFLDLVLGYFNPNDSQPQTLTRIHDITSHLPSSIEEYSVYSLLHERLWSLHSFEDIHEIFISLGNYIEGVYDSEEEAPHMLFSSSSLLSIFLRKCVVEYEQFSFEQGVQFFKSFLQYRAPSMDFQHEGIEFCKINVRHELMNLASNKTLLQLAFGSIPVSRSSRDIEQLTQVQIEHMQKFGCALPLEMKEKLHDLLEVEENNLNTSYYCKFLNSWFSGDYQQSVENLCRYFDHIMHSDEKVSYQYALLNLAMLQADFGCNEEALHAIEDTINTARESGDTACLNFALAWMFEFKRSQYSNPSPEKNENDDQILEHLARNSQKAQLPILQTTVHLLQVQKFLDDGSSLIGVFSNLTKAFALFCANESFDGFPRYMDLLQSIWYRLGCSILSMTYISIYQKNHVHDSPALDLLQSLITKAWRYAELGRWLDVATILSTAEVQSQRSFQCAHLYGREKTFIYLSKAIDEKNHMDATLYLKNLNGFRHDKMSTFRTQLYALRLDISLGNLSQAIEGIDKLLEEPEKLAIEWVLQLMFLKVDIYMRTEMSNRAVFELLEIIQMASSNYLRLYLLRGLCTLCLIGELDCFTAIDLLDSLIPDIIAADNKPLLALAFHAFASLQIKHLDLLQPSRMFVIQLLDSAIDGYRACYMVEMQKTVVRLKIQYLRQINDPALQPNIEQYCSRFYSDNLLN AF3A7475798EE831 apc4-apc1 3 23 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 28 unspecified role unspecified role bait bait region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey association association Table 2 lid1-apc2 1 20 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 25 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 30 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 26 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Table 1 swm1-apc1 3 5 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 27 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey association association Table 2 mnd2-apc1-1 3 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 27 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 24 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 28 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey association association Table 2 apc13-apc2 1 25 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 30 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 29 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 26 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey association association Table 1 apc13-lid1 4 26 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 B In an anti-myc immunoprecipitate from the lid1-myc apc13-HA strain (KGY3582), but not single-tagged strains (KGY1336 and KGY3398), both Lid1p-myc and Apc13p-HA were detected (Figure 1B), and, conversely, both Apc13p-HA and Lid1p-myc could be detected in an anti-HA immunoprecipitate lid1-apc13 4 26 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 B In an anti-myc immunoprecipitate from the lid1-myc apc13-HA strain (KGY3582), but not single-tagged strains (KGY1336 and KGY3398), both Lid1p-myc and Apc13p-HA were detected (Figure 1B), and, conversely, both Apc13p-HA and Lid1p-myc could be detected in an anti-HA immunoprecipitate cut9-apc14 4 20 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 C In Cut9p-HA immunoprecipitates from cut9-HA apc14-myc (KGY3587) and cut9-HA apc15-myc(KGY3665) strains, Apc14p-myc and Apc15p-myc were each detected and were absent from the single cut9-HA strain. In reciprocal experiments, anti-myc immunoprecipitates from the double-tagged strains contained Cut9p-HA apc14-cut9 4 20 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 C In Cut9p-HA immunoprecipitates from cut9-HA apc14-myc (KGY3587) and cut9-HA apc15-myc(KGY3665) strains, Apc14p-myc and Apc15p-myc were each detected and were absent from the single cut9-HA strain cut9-apc15 4 20 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 D In Cut9p-HA immunoprecipitates from cut9-HA apc14-myc (KGY3587) and cut9-HA apc15-myc(KGY3665) strains, Apc14p-myc and Apc15p-myc were each detected and were absent from the single cut9-HA strain. In reciprocal experiments, anti-myc immunoprecipitates from the double-tagged strains contained Cut9p-HA apc15-cut9 4 20 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 D In Cut9p-HA immunoprecipitates from cut9-HA apc14-myc (KGY3587) and cut9-HA apc15-myc(KGY3665) strains, Apc14p-myc and Apc15p-myc were each detected and were absent from the single cut9-HA strain. In reciprocal experiments, anti-myc immunoprecipitates from the double-tagged strains contained Cut9p-HA apc15-apc13 4 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 E We also tested whether these proteins associate with one another. To this end, apc13-HA apc15-myc (KGY3735) and apc15-HA apc14-myc (KGY1611) strains were constructed. Anti-HA immunoprecipitates from these strains contained Apc13p-HA and Apc15p-HA as expected and also contained Apc15p-myc and Apc14p-myc, respectively apc13-apc15-1 4 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 E We also tested whether these proteins associate with one another. To this end, apc13-HA apc15-myc (KGY3735) and apc15-HA apc14-myc (KGY1611) strains were constructed. Anti-HA immunoprecipitates from these strains contained Apc13p-HA and Apc15p-HA as expected and also contained Apc15p-myc and Apc14p-myc, respectively apc15-apc14 4 14 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 F We also tested whether these proteins associate with one another. To this end, apc13-HA apc15-myc (KGY3735) and apc15-HA apc14-myc (KGY1611) strains were constructed. Anti-HA immunoprecipitates from these strains contained Apc13p-HA and Apc15p-HA as expected and also contained Apc15p-myc and Apc14p-myc, respectively apc14-apc15 4 16 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 F We also tested whether these proteins associate with one another. To this end, apc13-HA apc15-myc (KGY3735) and apc15-HA apc14-myc (KGY1611) strains were constructed. Anti-HA immunoprecipitates from these strains contained Apc13p-HA and Apc15p-HA as expected and also contained Apc15p-myc and Apc14p-myc, respectively swm1-apc4 2 27 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 28 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 B, 3 D Anti-HA immunoprecipitates from the APC4-HA MND2-myc and SWM1-HA APC4-myc strains contained both the HA-tagged proteins as well as Mnd2p-myc and Apc4p-myc (Figures 3B and 3C). Reciprocally, anti-myc immunoprecipitates contained the myc-tagged proteins and also Apc4p-HA and Swm1p-HA, respectively This interaction occurred in cells blocked in G1, S and G2/M phases as well as control cells, suggesting it is not a cell-cycle specific interaction. apc4-swm1 2 27 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 28 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 B, 3 D Anti-HA immunoprecipitates from the APC4-HA MND2-myc and SWM1-HA APC4-myc strains contained both the HA-tagged proteins as well as Mnd2p-myc and Apc4p-myc (Figures 3B and 3C). Reciprocally, anti-myc immunoprecipitates contained the myc-tagged proteins and also Apc4p-HA and Swm1p-HA, respectively This interaction occurred in cells blocked in G1, S and G2/M phases as well as control cells, suggesting it is not a cell-cycle specific interaction. apc4-mnd2 2 13 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 28 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 C, 3 E Anti-HA immunoprecipitates from the APC4-HA MND2-myc and SWM1-HA APC4-myc strains contained both the HA-tagged proteins as well as Mnd2p-myc and Apc4p-myc (Figures 3B and 3C). Reciprocally, anti-myc immunoprecipitates contained the myc-tagged proteins and also Apc4p-HA and Swm1p-HA, respectively This interaction occurred in cells blocked in G1, S and G2/M phases as well as control cells, suggesting it is not a cell-cycle specific interaction. mnd2-apc4 2 13 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 28 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 C, 3 E Anti-HA immunoprecipitates from the APC4-HA MND2-myc and SWM1-HA APC4-myc strains contained both the HA-tagged proteins as well as Mnd2p-myc and Apc4p-myc (Figures 3B and 3C). Reciprocally, anti-myc immunoprecipitates contained the myc-tagged proteins and also Apc4p-HA and Swm1p-HA, respectively This interaction occurred in cells blocked in G1, S and G2/M phases as well as control cells, suggesting it is not a cell-cycle specific interaction.