IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Mol. Cell. Biol. (0270-7306) 2002 imex curation Krogan NJ., Kim M., Ahn SH., Zhong G., Kobor MS., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt JF. Cancer - Interactions investigated in the context of cancer TAPs were carried, proteins were purified, then analyzed by SDS-PAGE and silver staining, and identified by trypsin digestion and MALDI-TOF mass spectrometry. Part of cancer dataset as CKII is related to cancer. jack.greenblatt@utoronto.ca 23-MAY-2007: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Krogan NJ., Kim M., Ahn SH., Zhong G., Kobor MS., Cagney G., Emili A., Shilatifard A., Buratowski S., Greenblatt JF. Cancer - Interactions investigated in the context of cancer TAPs were carried, proteins were purified, then analyzed by SDS-PAGE and silver staining, and identified by trypsin digestion and MALDI-TOF mass spectrometry. Part of cancer dataset as CKII is related to cancer. jack.greenblatt@utoronto.ca Accepted 2007-JUN-16 by ORCHARD. 23-MAY-2007: Contacted by JYOTI. Mol. Cell. Biol. (0270-7306) 2002 orchard Only protein-protein interactions imex curation ctr9_yeast RNA polymerase-associated protein CTR9 CTR9 CDP1 Cln three-requiring protein 9 Centromere-binding factor 1-dependent protein 1 YOL145C protein protein yeast Saccharomyces cerevisiae Baker's yeast MTNAMKVEGYPSMEWPTSLDIPLKASEELVGIDLETDLPDDPTDLKTLLVEENSEKEHWLTIALAYCNHGKTNEGIKLIEMALDVFQNSERASLHTFLTWAHLNLAKGQSLSVETKEHELTQAELNLKDAIGFDPTWIGNMLATVELYYQRGHYDKALETSDLFVKSIHAEDHRSGRQSKPNCLFLLLRAKLLYQKKNYMASLKIFQELLVINPVLQPDPRIGIGLCFWQLKDSKMAIKSWQRALQLNPKNTSASILVLLGEFRESFTNSTNDKTFKEAFTKALSDLNNIFSENQHNPVLLTLLQTYYYFKGDYQTVLDIYHHRILKMSPMIAKIVLSESSFWCGRAHYALGDYRKSFIMFQESLKKNEDNLLAKLGLGQTQIKNNLLEESIITFENLYKTNESLQELNYILGMLYAGKAFDAKTAKNTSAKEQSNLNEKALKYLERYLKLTLATKNQLVISRAYLVISQLYELQNQYKTSLDYLSKALEEMEFIKKEIPLEVLNNLACYHFINGDFIKADDLFKQAKAKVSDKDESVNITLEYNIARTNEKNDCEKSESIYSQVTSLHPAYIAARIRNLYLKFAQSKIEDSDMSTEMNKLLDLNKSDLEIRSFYGWYLKNSKERKNNEKSTTHNKETLVKYNSHDAYALISLANLYVTIARDGKKSRNPKEQEKSKHSYLKAIQLYQKVLQVDPFNIFAAQGLAIIFAESKRLGPALEILRKVRDSLDNEDVQLNLAHCYLEMREYGKAIENYELVLKKFDNEKTRPHILNLLGRAWYARAIKERSVNFYQKALENAKTALDLFVKESSKSKFIHSVKFNIALLHFQIAETLRRSNPKFRTVQQIKDSLEGLKEGLELFRELNDLKEFNMIPKEELEQRIQLGETTMKSALERSLNEQEEFEKEQSAKIDEARKILEENELKEQGWMKQEEEARRLKLEKQAEEYRKLQDEAQKLIQEREAMAISEHNVKDDSDLSDKDNEYDEEKPRQKRKRSTKTKNSGESKRRKAAKKTLSDSDEDDDDVVKKPSHNKGKKSQLSNEFIEDSDEEEAQMSGSEQNKNDDNDENNDNDDNDGLF F794ECBAD26D4820 F794ECBAD26D4820 spt6_yeast Transcription elongation factor SPT6 CRE2 SPT6 SSN20 YGR116W G6169 Chromatin elongation factor SPT6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEETGDSKLVPRDEEEIVNDNDETKAPSEEEEGEDVFDSSEEDEDIDEDEDEARKVQEGFIVNDDDENEDPGTSISKKRRKHKRREREEDDRLSEDDLDLLMENAGVERTKASSSSGKFKRLKRVGDEGNAAESESDNVAASRQDSTSKLEDFFSEDEEEEESGLRNGRNNEYGRDEEDHENRNRTADKGGILDELDDFIEDDEFSDEDDETRQRRIQEKKLLREQSIKQPTQITGLSSDKIDEMYDIFGDGHDYDWALEIENEELENGNDNNEAEEEEIDEETGAIKSTKKKISLQDIYDLEDLKKNLMTEGDMKIRKTDIPERYQELRAGITDYGNMSSEDQELERNWIAEKISVDKNFDANYDLTEFKEAIGNAIKFITKENLEVPFIYAYRRNYISSREKDGFLLTEDDLWDIVSLDIEFHSLVNKKDYVQRFYAELHIDDPIVTEYFKNQNTASIAELNSLQDIYDYLEFKYANEINEMFINHTGKTGKKHLKNSSYEKFKASPLYQAVSDIGISAEDVGENISSQHQIHPPVDHPSSKPVEVIESILNANSGDLQVFTSNTKLAIDTVQKYYSLELSKNTKIREKVRSDFSKYYLADVVLTAKGKKEIQKGSLYEDIKYAINRTPMHFRRDPDVFLKMVEAESLNLLSVKLHMSSQAQYIEHLFQIALETTNTSDIAIEWNNFRKLAFNQAMDKIFQDISQEVKDNLTKNCQKLVAKTVRHKFMTKLDQAPFIPNVRDPKIPKILSLTCGQGRFGADAIIAVYVNRKGDFIRDYKIVDNPFDKTNPEKFEDTLDNIIQSCQPNAIGINGPNPKTQKFYKRLQEVLHKKQIVDSRGHTIPIIYVEDEVAIRYQNSERAAQEFPNKPPLVKYCIALARYMHSPLLEYANLTSEEVRSLSIHPHQNLLSSEQLSWALETAFVDIVNLVSVEVNKATDNNYYASALKYISGFGKRKAIDFLQSLQRLNEPLLARQQLITHNILHKTIFMNSAGFLYISWNEKRQKYEDLEHDQLDSTRIHPEDYHLATKVAADALEYDPDTIAEKEEQGTMSEFIELLREDPDRRAKLESLNLESYAEELEKNTGLRKLNNLNTIVLELLDGFEELRNDFHPLQGDEIFQSLTGESEKTFFKGSIIPVRVERFWHNDIICTTNSEVECVVNAQRHAGAQLRRPANEIYEIGKTYPAKVIYIDYANITAEVSLLDHDVKQQYVPISYSKDPSIWDLKQELEDAEEERKLMMAEARAKRTHRVINHPYYFPFNGRQAEDYLRSKERGEFVIRQSSRGDDHLVITWKLDKDLFQHIDIQELEKENPLALGKVLIVDNQKYNDLDQIIVEYLQNKVRLLNEMTSSEKFKSGTKKDVVKFIEDYSRVNPNKSVYYFSLNHDNPGWFYLMFKINANSKLYTWNVKLTNTGYFLVNYNYPSVIQLCNGFKTLLKSNSSKNRMNNYR 0BE9922A59BD0483 0BE9922A59BD0483 spt16_yeast FACT complex subunit SPT16 SPT16 CDC68 SSF1 YGL207W Facilitates chromatin transcription complex subunit SPT16 Suppressor of Ty protein 16 Cell division control protein 68 protein protein yeast Saccharomyces cerevisiae Baker's yeast MEELNIDFDVFKKRIELLYSKYNEFEGSPNSLLFVLGSSNAENPYQKTTILHNWLLSYEFPATLIALVPGKVIIITSSAKAKHLQKAIDLFKDPESKITLELWQRNNKEPELNKKLFDDVIALINSAGKTVGIPEKDSYQGKFMTEWNPVWEAAVKENEFNVIDISLGLSKVWEVKDVNEQAFLSVSSKGSDKFMDLLSNEMVRAVDEELKITNAKLSDKIENKIDDVKFLKQLSPDLSALCPPNYKFNFDLLDWTYSPIIQSGKKFDLRVSARSTNDQLYGNGCILASCGIRYNNYCSNITRTFLIDPSEEMANNYDFLLTLQKEIVTNILKPGRTPKEVYESVIEYIEKTKPELVPNFTKNIGSLIGLEFRDSNFILNVKNDYRKIQRGDCFNISFGFNNLKDSQSANNYALQLADTVQIPLDETEPPRFLTNYTKAKSQISFYFNNEEEDNNKKKSSPATKVPSKPDRNSKILRTKLRGEARGGAEDAQKEQIRKENQKKLHEKLEKNGLLRFSAADANGPDSEPRQYFKKYESYVRDSQLPTNIRDLRIHVDWKSQTIILPIYGRPVPFHINSYKNGSKNEEGEYTYLRLNFNSPGSSGGISKKVEELPYEESADNQFVRSITLRSKDGDRMSETFKQIADLKKEATKREQERKALADVVQQDKLIENKTGRTKRLDQIFVRPNPDTKRVPSTVFIHENGIRFQSPLRTDSRIDILFSNIKNLIFQSCKGELIVVIHIHLKNPILMGKKKIQDVQFYREASDMSVDETGGGRRGQSRFRRYGDEDELEQEQEERRKRAALDKEFKYFADAIAEASNGLLTVENTFRDLGFQGVPNRSAVFCMPTTDCLVQLIEPPFLVINLEEVEICILERVQFGLKNFDMVFVYKDFNKPVTHINTVPIESLDFLKQWLTDMDIPYTVSTINLNWATIMKSLQDDPYQFFLDGGWNFLATGSDDEASDESEEEVSEYEASEDDVSDESAFSEDEEGSEVDDDISGDESEDYTGDESEEGEDWDELEKKAARADRGANFRD 4F01C772E299E2E6 4F01C772E299E2E6 taf14_yeast Transcription initiation factor TFIID subunit 14 ANC1 CST10 YPL129W TAF14 SWP29 TAF30 TFG3 TBP-associated factor 14 TBP-associated factor 30 kDa Transcription initiation factor TFIIF 30 kDa subunit Transcription factor G 30 kDa subunit SWI/SNF chromatin-remodeling complex subunit TAF14 SWI/SNF complex subunit TAF14 SWI/SNF complex 29 kDa subunit Actin non-complementing mutant 1 Chromosome stability protein 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERKIPHDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGSTEETTANTGTIGKRRTTTNTTAEPKAKRAKTGSASTVKGSVDLEKLAFGLTKLNEDDLVGVVQMVTDNKTPEMNVTNNVEEGEFIIDLYSLPEGLLKSLWDYVKKNTE 60EA5EC2474B1B9C 60EA5EC2474B1B9C csk22_yeast Casein kinase II subunit alpha' CKA2 YOR29-12 YOR061W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPLPPSTLNQKSNRVYSVARVYKNACEERPQEYWDYEQGVTIDWGKISNYEIINKIGRGKYSEVFSGRCIVNNQKCVIKVLKPVKMKKIYRELKILTNLTGGPNVVGLYDIVQDADSKIPALIFEEIKNVDFRTLYPTFKLPDIQYYFTQLLIALDYCHSMGIMHRDVKPQNVMIDPTERKLRLIDWGLAEFYHPGVDYNVRVASRYHKGPELLVNLNQYDYSLDLWSVGCMLAAIVFKKEPFFKGSSNPDQLVKIATVLGTKELLGYLGKYGLHLPSEYDNIMRDFTKKSWTHFITSETKLAVPEVVDLIDNLLRYDHQERLTAKEAMDHKFFKTKFE 30E214BD852824D0 30E214BD852824D0 rtf1_yeast RNA polymerase-associated protein RTF1 RTF1 YGL244W HRA458 CSL3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLDEDLLALAGADESEEEDQVLTTTSAKRAKNNDQSLSKKRRIEVGSVEDDDEEDDYNPYSVGNADYGSEEEEEANPFPLEGKYKDESDREHLESLPEMERETLLFERSQIMQKYQERKLFRARGRDMKEQQQRAKNDEDSRKTRASTRSTHATGHSDIKASKLSQLKKQRARKNRHYSDNEDEDDEEDYREEDYKDDEGSEYGDDEEYNPFDRRDTYDKREEVEWAEEEDEQDREPEISDFNKLRIGRSFVAKFCFYPGFEDAVKGCYGRVNVGTDKRTGKTSYRMVRIERVFLQKPYNMGKFYTNQYFGVTQGKDRKVFQMNYFSDGLFAEDEYQRYLRALDNSQMIKPSLHSLSNKTKEVMDFVNTPLTDKTTDEVVRHRMQFNKKLSGTNAVLEKTVLREKLQYAKETNNEKDIAKYSAQLRNFEKRMSVYEKHHENDQSDIKKLGELTSKNRKLNMSNIRNAEHVKKEDSNNFDSKSDPFSRLKTRTKVYYQEIQKEENAKAKEIAQQEKLQEDKDAKDKREKELLVAQFRRLGGLERMVGELDIKFDLKF 13AECCEC6D63CED4 13AECCEC6D63CED4 csk2b_yeast Casein kinase II subunit beta CKB1 YGL019W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQEFVEDYSRTGSSDDEDSGAYDEWIPSFCSRFGHEYFCQVPTEFIEDDFNMTSLSQEVPHYRKALDLILDLEAMSDEEEDEDDVVEEDEVDQEMQSNDGHDEGKRRNKSPVVNKSIIEHAAEQLYGLIHARFILTKPGLQAMAEKFDHKEFGTCPRYYCNGMQLLPCGLSDTVGKHTVRLYCPSCQDLYLPQSSRFLCLEGAFWGTSFPGVFLKHFKELEEYVERKSKESYELKVFGFRINDEAVSGPRMKWLRQYPSTEEDWEEFAKCEFETPAV 9ACA8D285E6990AF 9ACA8D285E6990AF leo1_yeast RNA polymerase-associated protein LEO1 O3278 YOR3278C YOR123C LEO1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSESPQDQPQKEQISNNVGVTTNSTSNEETSRSQDDNVKEVNGNDDTKEEEQEEDAELDDLFGDDNDDDDDDDVKKSETEKSDSDSDEDDEGENINHRSRHRESLGLDDDEAEEQAMYTRKFYGEDANNFSDQDETTHTFKEENVELVRHIIPSKANVNETASHNEIFYARIPNFLTIDPIPFDPPSFEAKVNERASNSASREDQLDDRLIDENTVRWRYSRDKDQHVFKESNTQIVQWSDGTYSLKVGEECTDILVNDTSNTFLTVSHDQQELIQCYEGGEIKKTLMFIPTSTNSKIHQKLSKAVIRRNQRQSKGPGTYIVSMDPEVEKKELERKQSQILRDRRRRQLKEKEKQESPDAAFETGFRKQNSPTTYGASRRNEYEEDDFLVDDDEEEEAAFDDEEDDNEEEEEEEDADEENASRLRNLKREGAAMYREEEEEEKDRSETKRRRVAVIEDDEDED ABDE3D7D5A2C78A5 ABDE3D7D5A2C78A5 spt4_yeast Transcription elongation factor SPT4 YGR063C SPT4 Chromatin elongation factor SPT4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSPSFEGLVGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGSQVE 59C164193ABA883B 59C164193ABA883B iws1_yeast Transcription factor SPN1 SPN1 Interacts with SPT6 protein 1 Suppresses postrecruitment functions protein 1 IWS1 YPR133C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTADQEQPKVVEATPEDGTASSQKSTINAENENTKQNQSMEPQETSKGTSNDTKDPDNGEKNEEAAIDENSNVEAAERKRKHISTDFSDDDLEKEEHNDQSLQPTVENRASKDRDSSATPSSRQELEEKLDRILKKPKVRRTRRDEDDLEQYLDEKILRLKDEMNIAAQLDIDTLNKRIETGDTSLIAMQKVKLLPKVVSVLSKANLADTILDNNLLQSVRIWLEPLPDGSLPSFEIQKSLFAALNDLPVKTEHLKESGLGRVVIFYTKSKRVEAQLARLAEKLIAEWTRPIIGASDNYRDKRIMQLEFDSEKLRKKSVMDSAKNRKKKSKSGEDPTSRGSSVQTLYEQAAARRNRAAAPAQTTTDYKYAPVSNLSAVPTNARAVGVGSTLNNSEMYKRLTSRLNKKHK 7BC925FC8BA80E65 7BC925FC8BA80E65 cdc73_yeast Cell division control protein 73 RNA polymerase-associated protein CDC73 CDC73 YLR418C protein protein yeast Saccharomyces cerevisiae Baker's yeast MANSLDRLREHLKNGDKLVLKNNEGQSTDDITKATMVETLSSDGSTQDSFPLNEETEIEIDGSLVQLRIIVHCWMNKDSSAADYLADCQNKQLTNVSFLQRTDLINWLSGNTESSQYLKAPGQKGETSDKVDIENKTLAGELSTVKSTTSASLENDSEVSDPVVVETMKHERILVDHNSALRGAKPINFGYLIKDAELKLVQSIKGSLRGSKLPPGHKGAHGRISKTNGSSGGPRKDPIILIPSAASSILTVANIKQFLLESKYVNPRNLPSVPNGLVNIEKNFERISRPIRFIIVDNTRMFTKPEYWDRVVAIFTTGHTWQFNNYQWNSPQELFQRCKGYYFHFAGDSVPQHVQQWNVEKVELDKNKRFKDVEVVRYFWHSLEKELISRGYR 9580B53EEFB75345 9580B53EEFB75345 psh1_yeast RING finger protein PSH1 PSH1 POB3/SPT16 histone-associated protein 1 O1263 YOL054W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGDELHNRLLHQNDGTKDAILYKIIESLVCSICHDYMFVPMMTPCGHNYCYGCLNTWFASNTQKELACPQCRSDITTIPALNTTLQQYLSFILEKLRDQNDESFKKLLTTKTKEENDYKNDKEKDTLFDKVFKNSALAVADDSDDGITRCSNCHWELDPDEVEDGNVCPHCNARIRNYAGGRDEFDEEEYSEGELDEIRESMRRRRENRFASTNPFANRDDVSSEDDDSSEEEPMREHIPLGRWARSHNRSIAVDAVDDEDDEEEDEEEEEEMDSDLKDFIEDDEDDEDEDGSRRNLVLSALKNRHVIITDDEEEEQRRHATEEEDRDSDFYEHNDDGFVSGDSLDEDQKEVTRIQSSSDSEDRSLSYSGSSDVKDNNDDNTEELDDPQPKRQKRFRVVLGDSDDE CE0ECBCB6D465554 CE0ECBCB6D465554 rpb1_yeast DNA-directed RNA polymerase II subunit RPB1 RPO21 RPB220 RPB1 SUA8 YDL140C D2150 DNA-directed RNA polymerase III largest subunit RNA polymerase II subunit B220 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVGQQYSSAPLRTVKEVQFGLFSPEEVRAISVAKIRFPETMDETQTRAKIGGLNDPRLGSIDRNLKCQTCQEGMNECPGHFGHIDLAKPVFHVGFIAKIKKVCECVCMHCGKLLLDEHNELMRQALAIKDSKKRFAAIWTLCKTKMVCETDVPSEDDPTQLVSRGGCGNTQPTIRKDGLKLVGSWKKDRATGDADEPELRVLSTEEILNIFKHISVKDFTSLGFNEVFSRPEWMILTCLPVPPPPVRPSISFNESQRGEDDLTFKLADILKANISLETLEHNGAPHHAIEEAESLLQFHVATYMDNDIAGQPQALQKSGRPVKSIRARLKGKEGRIRGNLMGKRVDFSARTVISGDPNLELDQVGVPKSIAKTLTYPEVVTPYNIDRLTQLVRNGPNEHPGAKYVIRDSGDRIDLRYSKRAGDIQLQYGWKVERHIMDNDPVLFNRQPSLHKMSMMAHRVKVIPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPCMGIVQDTLCGIRKLTLRDTFIELDQVLNMLYWVPDWDGVIPTPAIIKPKPLWSGKQILSVAIPNGIHLQRFDEGTTLLSPKDNGMLIIDGQIIFGVVEKKTVGSSNGGLIHVVTREKGPQVCAKLFGNIQKVVNFWLLHNGFSTGIGDTIADGPTMREITETIAEAKKKVLDVTKEAQANLLTAKHGMTLRESFEDNVVRFLNEARDKAGRLAEVNLKDLNNVKQMVMAGSKGSFINIAQMSACVGQQSVEGKRIAFGFVDRTLPHFSKDDYSPESKGFVENSYLRGLTPQEFFFHAMGGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDNTTRNSLGNVIQFIYGEDGMDAAHIEKQSLDTIGGSDAAFEKRYRVDLLNTDHTLDPSLLESGSEILGDLKLQVLLDEEYKQLVKDRKFLREVFVDGEANWPLPVNIRRIIQNAQQTFHIDHTKPSDLTIKDIVLGVKDLQENLLVLRGKNEIIQNAQRDAVTLFCCLLRSRLATRRVLQEYRLTKQAFDWVLSNIEAQFLRSVVHPGEMVGVLAAQSIGEPATQMTLNTFHFAGVASKKVTSGVPRLKEILNVAKNMKTPSLTVYLEPGHAADQEQAKLIRSAIEHTTLKSVTIASEIYYDPDPRSTVIPEDEEIIQLHFSLLDEEAEQSFDQQSPWLLRLELDRAAMNDKDLTMGQVGERIKQTFKNDLFVIWSEDNDEKLIIRCRVVRPKSLDAETEAEEDHMLKKIENTMLENITLRGVENIERVVMMKYDRKVPSPTGEYVKEPEWVLETDGVNLSEVMTVPGIDPTRIYTNSFIDIMEVLGIEAGRAALYKEVYNVIASDGSYVNYRHMALLVDVMTTQGGLTSVTRHGFNRSNTGALMRCSFEETVEILFEAGASAELDDCRGVSENVILGQMAPIGTGAFDVMIDEESLVKYMPEQKITEIEDGQDGGVTPYSNESGLVNADLDVKDELMFSPLVDSGSNDAMAGGFTAYGGADYGEATSPFGAYGEAPTSPGFGVSSPGFSPTSPTYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPAYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPNYSPTSPSYSPTSPGYSPGSPAYSPKQDEQKHNENENSR A45C1360FF99F968 A45C1360FF99F968 paf1_yeast RNA polymerase II-associated protein 1 PAF1 YBR279W YBR2016 Protein PAF1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSKKQEYIAPIKYQNSLPVPQLPPKLLVYPESPETNADSSQLINSLYIKTNVTNLIQQDEDLGMPVDLMKFPGLLNKLDSKLLYGFDNVKLDKDDRILLRDPRIDRLTKTDISKVTFLRRTEYVSNTIAAHDNTSLKRKRRLDDGDSDDENLDVNHIISRVEGTFNKTDKWQHPVKKGVKMVKKWDLLPDTASMDQVYFILKFMGSASLDTKEKKSLNTGIFRPVELEEDEWISMYATDHKDSAILENELEKGMDEMDDDSHEGKIYKFKRIRDYDMKQVAEKPMTELAIRLNDKDGIAYYKPLRSKIELRRRRVNDIIKPLVKEHDIDQLNVTLRNPSTKEANIRDKLRMKFDPINFATVDEEDDEDEEQPEDVKKESEGDSKTEGSEQEGENEKDEEIKQEKENEQDEENKQDENRAADTPETSDAVHTEQKPEEEKETLQEE 46FB5AB37C3C1AAE csk2c_yeast Casein kinase II subunit beta' CKB2 OR26.32 YOR039W protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSRSENVGTVTREGSRVEQDDVLMDDDSDSSEYVDMWIDLFLGRKGHEYFCDVDPEYITDRFNLMNLQKTVSKFSYVVQYIVDDLDDSILENMTHARLEQLESDSRKLYGLIHARYIITIKGLQKMYAKYKEADFGRCPRVYCNLQQLLPVGLHDIPGIDCVKLYCPSCEDLYIPKSSRHSSIDGAYFGTSFPGMFLQAFPDMVPKHPTKRYVPKIFGFELHKQAQLTRWQELQRLKLVEKLESKDVDLTKSGGFKT D6060CB3D70B19A4 D6060CB3D70B19A4 t2fb_yeast Transcription initiation factor IIF subunit beta YGR005C TFG2 TFIIF-beta TFIIF medium subunit Transcription factor G 54 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSGSAGAPALSNNSTNSVAKEKSGNISGDEYLSQEEEVFDGNDIENNETKVYEESLDLDLERSNRQVWLVRLPMFLAEKWRDRNNLHGQELGKIRINKDGSKITLLLNENDNDSIPHEYDLELTKKVVENEYVFTEQNLKKYQQRKKELEADPEKQRQAYLKKQEREEELKKKQQQQKRRNNRKKFNHRVMTDRDGRDRYIPYVKTIPKKTAIVGTVCHECQVMPSMNDPNYHKIVEQRRNIVKLNNKERITTLDETVGVTMSHTGMSMRSDNSNFLKVGREKAKSNIKSIRMPKKEILDYLFKLFDEYDYWSLKGLKERTRQPEAHLKECLDKVATLVKKGPYAFKYTLRPEYKKLKEEERKATLGELADEQTGSAGDNAQGDAEADLEDEIEMEDVV DDDA895446BBF26F DDDA895446BBF26F chd1_yeast Chromo domain-containing protein 1 CHD1 YER164W SYGP-ORF4 ATP-dependent helicase CHD1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAAKDISTEVLQNPELYGLRRSHRAAAHQQNYFNDSDDEDDEDNIKQSRRKRMTTIEDDEDEFEDEEGEEDSGEDEDEEDFEEDDDYYGSPIKQNRSKPKSRTKSKSKSKPKSQSEKQSTVKIPTRFSNRQNKTVNYNIDYSDDDLLESEDDYGSEEALSEENVHEASANPQPEDFHGIDIVINHRLKTSLEEGKVLEKTVPDLNNCKENYEFLIKWTDESHLHNTWETYESIGQVRGLKRLDNYCKQFIIEDQQVRLDPYVTAEDIEIMDMERERRLDEFEEFHVPERIIDSQRASLEDGTSQLQYLVKWRRLNYDEATWENATDIVKLAPEQVKHFQNRENSKILPQYSSNYTSQRPRFEKLSVQPPFIKGGELRDFQLTGINWMAFLWSKGDNGILADEMGLGKTVQTVAFISWLIFARRQNGPHIIVVPLSTMPAWLDTFEKWAPDLNCICYMGNQKSRDTIREYEFYTNPRAKGKKTMKFNVLLTTYEYILKDRAELGSIKWQFMAVDEAHRLKNAESSLYESLNSFKVANRMLITGTPLQNNIKELAALVNFLMPGRFTIDQEIDFENQDEEQEEYIHDLHRRIQPFILRRLKKDVEKSLPSKTERILRVELSDVQTEYYKNILTKNYSALTAGAKGGHFSLLNIMNELKKASNHPYLFDNAEERVLQKFGDGKMTRENVLRGLIMSSGKMVLLDQLLTRLKKDGHRVLIFSQMVRMLDILGDYLSIKGINFQRLDGTVPSAQRRISIDHFNSPDSNDFVFLLSTRAGGLGINLMTADTVVIFDSDWNPQADLQAMARAHRIGQKNHVMVYRLVSKDTVEEEVLERARKKMILEYAIISLGVTDGNKYTKKNEPNAGELSAILKFGAGNMFTATDNQKKLEDLNLDDVLNHAEDHVTTPDLGESHLGGEEFLKQFEVTDYKADIDWDDIIPEEELKKLQDEEQKRKDEEYVKEQLEMMNRRDNALKKIKNSVNGDGTAANSDSDDDSTSRSSRRRARANDMDSIGESEVRALYKAILKFGNLKEILDELIADGTLPVKSFEKYGETYDEMMEAAKDCVHEEEKNRKEILEKLEKHATAYRAKLKSGEIKAENQPKDNPLTRLSLKKREKKAVLFNFKGVKSLNAESLLSRVEDLKYLKNLINSNYKDDPLKFSLGNNTPKPVQNWSSNWTKEEDEKLLIGVFKYGYGSWTQIRDDPFLGITDKIFLNEVHNPVAKKSASSSDTTPTPSKKGKGITGSSKKVPGAIHLGRRVDYLLSFLRGGLNTKSPSADIGSKKLPTGPSKKRQRKPANHSKSMTPEITSSEPANGPPSKRMKALPKGPAALINNTRLSPNSPTPPLKSKVSRDNGTRQSSNPSSGSAHEKEYDSMDEEDCRHTMSAIRTSLKRLRRGGKSLDRKEWAKILKTELTTIGNHIESQKGSSRKASPEKYRKHLWSYSANFWPADVKSTKLMAMYDKITESQKK 78BDB74C7FEC6BE5 78BDB74C7FEC6BE5 t2fa_yeast Transcription initiation factor IIF subunit alpha TFG1 SSU71 YGR186W G7526 TFIIF large subunit Transcription factor G 105 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSRRNPPGSRNGGGPTNASPFIKRDRMRRNFLRMRMGQNGSNSSSPGVPNGDNSRGSLVKKDDPEYAEEREKMLLQIGVEADAGRSNVKVKDEDPNEYNEFPLRAIPKEDLENMRTHLLKFQSKKKINPVTDFHLPVRLHRKDTRNLQFQLTRAEIVQRQKEISEYKKKAEQERSTPNSGGMNKSGTVSLNNTVKDGSQTPTVDSVTKDNTANGVNSSIPTVTGSSVPPASPTTVSAVESNGLSNGSTSAANGLDGNASTANLANGRPLVTKLEDAGPAEDPTKVGMVKYDGKEVTNEPEFEEGTMDPLADVAPDGGGRAKRGNLRRKTRQLKVLDENAKKLRFEEFYPWVMEDFDGYNTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKVYKFTARNKYATLTIDEAEKRMDKKSGEVPRWLMKHLDNIGTTTTRYDRTRRKLKAVADQQAMDEDDRDDNSEVELDYDEEFADDEEAPIIDGNEQENKESEQRIKKEMLQANAMGLRDEEAPSENEEDELFGEKKIDEDGERIKKALQKTELAALYSSDENEINPYLSESDIENKENESPVKKEEDSDTLSKSKRSSPKKQQKKATNAHVHKEPTLRVKSIKNCVIILKGDKKILKSFPEGEWNPQTTKAVDSSNNASNTVPSPIKQEEGLNSTVAEREETPAPTITEKDIIEAIGDGKVNIKEFGKFIRRKYPGAENKKLMFAIVKKLCRKVGNDHMELKKE A6D4685F187BB593 A6D4685F187BB593 pob3_yeast FACT complex subunit POB3 YML069W Facilitates chromatin transcription complex subunit POB3 POB3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTDFDRIYLNQSKFSGRFRIADSGLGWKISTSGGSAANQARKPFLLPATELSTVQWSRGCRGYDLKINTKNQGVIQLDGFSQDDYNLIKNDFHRRFNIQVEQREHSLRGWNWGKTDLARNEMVFALNGKPTFEIPYARINNTNLTSKNEVGIEFNIQDEEYQPAGDELVEMRFYIPGVIQTNVDENMTKKEESSNEVVPKKEDGAEGEDVQMAVEEKSMAEAFYEELKEKADIGEVAGDAIVSFQDVFFTTPRGRYDIDIYKNSIRLRGKTYEYKLQHRQIQRIVSLPKADDIHHLLVLAIEPPLRQGQTTYPFLVLQFQKDEETEVQLNLEDEDYEENYKDKLKKQYDAKTHIVLSHVLKGLTDRRVIVPGEYKSKYDQCAVSCSFKANEGYLYPLDNAFFFLTKPTLYIPFSDVSMVNISRAGQTSTSSRTFDLEVVLRSNRGSTTFANISKEEQQLLEQFLKSKNLRVKNEDREVQERLQTALGSDSDEEDINMGSAGEDDESVDEDFQVSSDNDADEVAEEFDSDAALSDAEGGSDEERPSKKPKVE 02C04F620C7B1450 02C04F620C7B1450 spt5_yeast Transcription elongation factor SPT5 SPT5 YML010W YM9571.08 Chromatin elongation factor SPT5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDNSDTNVSMQDHDQQFADPVVVPQSTDTKDENTSDKDTVDSGNVTTTESTERAESTSNIPPLDGEEKEAKSEPQQPEDNAETAATEQVSSSNGPATDDAQATLNTDSSEANEIVKKEEGSDERKRPREEDTKNSDGDTKDEGDNKDEDDDEDDDDDDDDEDDDDEAPTKRRRQERNRFLDIEAEVSDDEDEDEDEEDSELVREGFITHGDDEDDEASAPGARRDDRLHRQLDQDLNKTSEEDAQRLAKELRERYGRSSSKQYRAAAQDGYVPQRFLLPSVDTATIWGVRCRPGKEKELIRKLLKKKFNLDRAMGKKKLKILSIFQRDNYTGRIYIEAPKQSVIEKFCNGVPDIYISQKLLIPVQELPLLLKPNKSDDVALEEGSYVRIKRGIYKGDLAMVDQISENNLEVMLKIVPRLDYGKFDEIDPTTQQRKSRRPTFAHRAPPQLFNPTMALRLDQANLYKRDDRHFTYKNEDYIDGYLYKSFRIQHVETKNIQPTVEELARFGSKEGAVDLTSVSQSIKKAQAAKVTFQPGDRIEVLNGEQRGSKGIVTRTTKDIATIKLNGFTTPLEFPISTLRKIFEPGDHVTVINGEHQGDAGLVLMVEQGQVTFMSTQTSREVTITANNLSKSIDTTATSSEYALHDIVELSAKNVACIIQAGHDIFKVIDETGKVSTITKGSILSKINTARARVSSVDANGNEIKIGDTIVEKVGSRREGQVLYIQTQQIFVVSKKIVENAGVFVVNPSNVEAVASKDNMLSNKMDLSKMNPEIISKMGPPSSKTFQQPIQSRGGREVALGKTVRIRSAGYKGQLGIVKDVNGDKATVELHSKNKHITIDKHKLTYYNREGGEGITYDELVNRRGRVPQARMGPSYVSAPRNMATGGIAAGAAATSSGLSGGMTPGWSSFDGGKTPAVNAHGGSGGGGVSSWGGASTWGGQGNGGASAWGGAGGGASAWGGQGTGATSTWGGASAWGNKSSWGGASTWASGGESNGAMSTWGGTGDRSAYGGASTWGGNNNNKSTRDGGASAWGNQDDGNRSAWNNQGNKSNYGGNSTWGGH E4324DB6B1E4721A E4324DB6B1E4721A csk21_yeast Casein kinase II subunit alpha YIL035C CKA1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKCRVWSEARVYTNINKQRTEEYWDYENTVIDWSTNTKDYEIENKVGRGKYSEVFQGVKLDSKVKIVIKMLKPVKKKKIKREIKILTDLSNEKVPPTTLPFQKDQYYTNQKEDVLKFIRPYIFDQPHNGHANIIHLFDIIKDPISKTPALVFEYVDNVDFRILYPKLTDLEIRFYMFELLKALDYCHSMGIMHRDVKPHNVMIDHKNKKLRLIDWGLAEFYHVNMEYNVRVASRFFKGPELLVDYRMYDYSLDLWSFGTMLASMIFKREPFFHGTSNTDQLVKIVKVLGTSDFEKYLLKYEITLPREFYDMDQYIRKPWHRFINDGNKHLSGNDEIIDLIDNLLRYDHQERLTAKEAMGHPWFAPIREQIEK 6A1A7F1BF47250C9 6A1A7F1BF47250C9 rpb2_yeast DNA-directed RNA polymerase II subunit RPB2 YOR151C RPB2 RPB150 RPO22 DNA-directed RNA polymerase II 140 kDa polypeptide B150 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDLANSEKYYDEDPYGFEDESAPITAEDSWAVISAFFREKGLVSQQLDSFNQFVDYTLQDIICEDSTLILEQLAQHTTESDNISRKYEISFGKIYVTKPMVNESDGVTHALYPQEARLRNLTYSSGLFVDVKKRTYEAIDVPGRELKYELIAEESEDDSESGKVFIGRLPIMLRSKNCYLSEATESDLYKLKECPFDMGGYFIINGSEKVLIAQERSAGNIVQVFKKAAPSPISHVAEIRSALEKGSRFISTLQVKLYGREGSSARTIKATLPYIKQDIPIVIIFRALGIIPDGEILEHICYDVNDWQMLEMLKPCVEDGFVIQDRETALDFIGRRGTALGIKKEKRIQYAKDILQKEFLPHITQLEGFESRKAFFLGYMINRLLLCALDRKDQDDRDHFGKKRLDLAGPLLAQLFKTLFKKLTKDIFRYMQRTVEEAHDFNMKLAINAKTITSGLKYALATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLMSCISVGTDPMPIITFLSEWGMEPLEDYVPHQSPDATRVFVNGVWHGVHRNPARLMETLRTLRRKGDINPEVSMIRDIREKELKIFTDAGRVYRPLFIVEDDESLGHKELKVRKGHIAKLMATEYQDIEGGFEDVEEYTWSSLLNEGLVEYIDAEEEESILIAMQPEDLEPAEANEENDLDVDPAKRIRVSHHATTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQKPLGTTRAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKKYGMSITETFEKPQRTNTLRMKHGTYDKLDDDGLIAPGVRVSGEDVIIGKTTPISPDEEELGQRTAYHSKRDASTPLRSTENGIVDQVLVTTNQDGLKFVKVRVRTTKIPQIGDKFASRHGQKGTIGITYRREDMPFTAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVAALSGNEGDASPFTDITVEGISKLLREHGYQSRGFEVMYNGHTGKKLMAQIFFGPTYYQRLRHMVDDKIHARARGPMQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAASFLKERLMEASDAFRVHICGICGLMTVIAKLNHNQFECKGCDNKIDIYQIHIPYAAKLLFQELMAMNITPRLYTDRSRDF BABD03212C0A583E BABD03212C0A583E spt5-rbp1 1 3 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 21 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Copurifying with the tagged Spt5 were Spt4, a substoichiometric amount of RNAPII, and, surprisingly, a truncated form of Spt6. The small untagged Spt4 polypeptide ran off the gel shown in Fig. 1A, but the presence of Spt4 in the sample applied to the gel was confirmed by tandem mass spectrometry. 1 A A truncated form of Spt6 copurifies with TAP-Spt5. spt4-rbp1 1 10 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey association association 1 A Purification of tagged Spt4 from yeast extracts in the presence of 100 mM NaCl resulted in copurification of three forms of Spt5 as well as a substoichiometric amount of RNAPII (Fig. 1A). The three electrophoretically distinct forms of Spt5 were consistently observed, but the suthors state that they were probably caused by C-terminal degradation, because the tagging and purification of Spt5 resulted in the isolation of only one form of tagged Spt5. spt6-iws1 1 11 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Tagging and purification of yeast Spt6 resulted in copurification of substoichiometric amounts of a previously uncharacterized protein, encoded by the essential open reading frame (ORF) YPR133c, which we have called Iws1 (interacts with Spt6) (Fig. 1B). 1 B iws1-spt6 1 3 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1 C Conversely, tagging and purification of Iws1 resulted in the isolation of Spt6, further confirming that this interaction occurs in vivo (Fig. 1C). There were three electrophoretically distinct forms of tagged Spt6, but only the form with the lowest electrophoretic mobility copurified with tagged Iws1. pob3-spt16 1 20 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association 3 A When the yeast counterparts of FACT, Spt16 and Pob3, were tagged and subsequently purified from yeast extracts in buffers containing 150 mM NaCl, large and approximately stoichiometric amounts of Spt16 and Pob3 were isolated and no other proteins were detected by silver staining (Fig. 3A). An increased number of proteins copurifying with Pob3-TAP was observed purification was carried out in buffers containing 125 mM NaCl, whereas when 150 mM NaCl was used only Spt16 copurified. spt16-pob3 1 20 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 A When the yeast counterparts of FACT, Spt16 and Pob3, were tagged and subsequently purified from yeast extracts in buffers containing 150 mM NaCl, large and approximately stoichiometric amounts of Spt16 and Pob3 were isolated and no other proteins were detected by silver staining (Fig. 3A). spt16-cdc73 1 4 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey association association Tandem and MALDI-TOF mass spectrometry were used to identify the following proteins that copurified with Spt16 and Pob3: Chd1, Ctr9, Paf1, Cdc73, Rtf1, CkaI, CkaII, CkbI, CkbII, histones, and proteins encoded by two uncharacterized ORFs, YOL054w and YOR123c (also known as LEO1) 3 C ymg9-cdc73-1 1 7 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 20 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey association association Tandem and MALDI-TOF mass spectrometry were used to identify the following proteins that copurified with Spt16 and Pob3: Chd1, Ctr9, Paf1, Cdc73, Rtf1, CkaI, CkaII, CkbI, CkbII, histones, and proteins encoded by two uncharacterized ORFs, YOL054w and YOR123c (also known as LEO1) 3 D The increased number of proteins copurifying with Pob3-TAP was observed purification was carried out in buffers containing 125 mM NaCl, whereas when 150 mM NaCl was used only Spt16 copurified. chd1-cka1-1 1 4 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role prey prey association association 3 E Tagging of Chd1 resulted in copurification of approximately stoichiometric amounts of the four subunits of CKII (CkaI, CkaII, CkbI, and CkbII), as well as substoichiometric amounts of Spt16 and Pob3 (Fig. 3E) rtf1-cdc73-1 1 12 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3 F These results indicated that there are at least three separable complexes that associate with Spt16 and Pob3: histones and nucleosomes, Chd1/CKII, and the complex containing Ctr9, Paf1, Cdc73, Rtf1, and Leo1. Confirmation came from experiments in which we labeled and purified Leo1 and Rtf1 (Fig. 3F), ctr9-cdc73-1 1 2 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey association association 3 F Tagging and purification of Ctr9 resulted in the coisolation of Rtf1, Leo1, Paf1, and Cdc73, as well as Spt16 and Pob3, but neither the histones nor CKII and Chd1 (Fig. 3F). leo1-cdc73 1 4 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey association association 3 F These results indicated that there are at least three separable complexes that associate with Spt16 and Pob3: histones and nucleosomes, Chd1/CKII, and the complex containing Ctr9, Paf1, Cdc73, Rtf1, and Leo1. Confirmation came from experiments in which we labeled and purified Leo1 and Rtf1 (Fig. 3F), paf1-cdc73-1 1 9 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey 2 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey association association 3 G These results indicated that there are at least three separable complexes that associate with Spt16 and Pob3: histones and nucleosomes, Chd1/CKII, and the complex containing Ctr9, Paf1, Cdc73, Rtf1, and Leo1. Confirmation came from experiments in which we labeled and purified Leo1 and Rtf1 (Fig. 3F), Paf1 (Fig. 3G) cdc73-rtf1-1 1 2 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey association association 3 H These results indicated that there are at least three separable complexes that associate with Spt16 and Pob3: histones and nucleosomes, Chd1/CKII, and the complex containing Ctr9, Paf1, Cdc73, Rtf1, and Leo1. Confirmation came from experiments in which we labeled and purified Leo1 and Rtf1 (Fig. 3F), Paf1 (Fig. 3G), and Cdc73(Fig. 3H). taf14-rpb1 1 14 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4 A tfg2-rpb1-2 1 5 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey association association 4 A tfg1-rpb1 1 5 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4 A