IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). Oncogene (0950-9232) 2002 Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov 23-JAN-2007: Contacted by JYOTI. A human fetal brain Matchmaker cDNA library (Clontech) was used. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov Accepted 2007-JAN-8 by JYOTI. 23-JAN-2007: Contacted by JYOTI. A human fetal brain Matchmaker cDNA library (Clontech) was used. Oncogene (0950-9232) 2002 Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). Oncogene (0950-9232) 2002 Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov 23-JAN-2007: Contacted by JYOTI. A human fetal brain Matchmaker cDNA library (Clontech) was used. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov Accepted 2007-JAN-8 by JYOTI. 23-JAN-2007: Contacted by JYOTI. A human fetal brain Matchmaker cDNA library (Clontech) was used. Oncogene (0950-9232) 2002 Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). Interaction and colocalization of PGP9.5 with JAB1 and p27(Kip1). Oncogene (0950-9232) 2002 Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov 23-JAN-2007: Contacted by JYOTI. A human fetal brain Matchmaker cDNA library (Clontech) was used. human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Caballero OL., Resto V., Patturajan M., Meerzaman D., Guo MZ., Engles J., Yochem R., Ratovitski E., Sidransky D., Jen J. Cancer - Interactions investigated in the context of cancer jenj@mail.nih.gov Accepted 2007-JAN-8 by JYOTI. 23-JAN-2007: Contacted by JYOTI. A human fetal brain Matchmaker cDNA library (Clontech) was used. Oncogene (0950-9232) 2002 ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS DIP protein P63279 original sequence version: 33 DIP protein P63279 original sequence version: 57 DIP protein P63279 original sequence version: 80 DIP protein P63279 original sequence version: 137 DIP protein P63279 original sequence version: 99 DIP protein P63279 original sequence version: 140 DIP protein P63279 original sequence version: 120 DIP protein P63279 original sequence version: 117 DIP protein P63279 original sequence version: 93 E2C826E9C8D0683D ranb9_human Ran-binding protein 9 RanBP7 Ran-binding protein M BPM90 BPM-L RANBP9 RANBPM protein protein human Homo sapiens Human MSGQPPPPPPQQQQQQQQLSPPPPAALAPVSGVVLPAPPAVSAGSSPAGSPGGGAGGEGLGAAAAALLLHPPPPPPPATAAPPPPPPPPPPPASAAAPASGPPAPPGLAAGPGPAGGAPTPALVAGSSAAAPFPHGDSALNEQEKELQRRLKRLYPAVDEQETPLPRSWSPKDKFSYIGLSQNNLRVHYKGHGKTPKDAASVRATHPIPAACGIYYFEVKIVSKGRDGYMGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDVIGCCVNLINNTCFYTKNGHSLGIAFTDLPPNLYPTVGLQTPGEVVDANFGQHPFVFDIEDYMREWRTKIQAQIDRFPIGDREGEWQTMIQKMVSSYLVHHGYCATAEAFARSTDQTVLEELASIKNRQRIQKLVLAGRMGEAIETTQQLYPSLLERNPNLLFTLKVRQFIEMVNGTDSEVRCLGGRSPKSQDSYPVSPRPFSSPSMSPSHGMNIHNLASGKGSTAHFSGFESCSNGVISNKAHQSYCHSNKHQSSNLNVPELNSINMSRSQQVNNFTSNDVDMETDHYSNGVGETSSNGFLNGSSKHDHEMEDCDTEMEVDSSQLRRQLCGGSQAAIERMIHFGRELQAMSEQLRRDCGKNTANKKMLKDAFSLLAYSDPWNSPVGNQLDPIQREPVCSALNSAILETHNLPKQPPLALAMGQATQCLGLMARSGIGSCAFATVEDYLH 50DF1127B7FDA6C8 cdn1b_human Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 p27Kip1 CDKN1B KIP1 protein protein human Homo sapiens Human MSNVRVSNGSPSLERMDARQAEHPKPSACRNLFGPVDHEELTRDLEKHCRDMEEASQRKWNFDFQNHKPLEGKYEWQEVEKGSLPEFYYRPPRPPKGACKVPAQESQDVSGSRPAAPLIGAPANSEDTHLVDPKTDPSDSQTGLAEQCAGIRKRPATDDSSTQNKRANRTEENVSDGSPNAGSVEQTPKKPGLRRRQT DIP protein P46527 original sequence version: 120 DIP protein P46527 original sequence version: 115 DIP protein P46527 original sequence version: 154 DIP protein P46527 original sequence version: 168 DIP protein P46527 original sequence version: 164 DIP protein P46527 original sequence version: 118 1118D58901CDF3FC csn5_human COP9 signalosome complex subunit 5 Jun activation domain-binding protein 1 COPS5 CSN5 JAB1 protein protein human Homo sapiens Human MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS DIP protein Q92905 original sequence version: 161 DIP protein Q92905 original sequence version: 87 DIP protein Q92905 original sequence version: 132 DIP protein Q92905 original sequence version: 149 DIP protein Q92905 original sequence version: 136 DIP protein Q92905 original sequence version: 101 B5742F4AAD03A1CF uchl1_human Ubiquitin carboxyl-terminal hydrolase isozyme L1 Ubiquitin thioesterase L1 Neuron cytoplasmic protein 9.5 PGP 9.5 UCHL1 protein protein human Homo sapiens Human MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA DIP protein P09936 original sequence version: 135 C9E972AC4DA5DA8A fbrl_human rRNA 2'-O-methyltransferase fibrillarin FBL FIB1 FLRN Histone-glutamine methyltransferase 34 kDa nucleolar scleroderma antigen U6 snRNA 2'-O-methyltransferase fibrillarin protein protein human Homo sapiens Human MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGRGGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKEDALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN DIP protein P22087 original sequence version: 84 DIP protein P22087 original sequence version: 154 2123F41E01EC557A uchl1-cops5 1 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association The fourth clone had a 1.05 kb insert identical to a human Jun activation domain- binding protein that was initially called p38JAB1 uchl1-ube2i 1 4 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association two of the clones had an identical 1.4-kb insert with 100% homology to the human ubiquitin-conjugating enzyme UBC9 uchl1-ranbp9 1 8 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Another clone with a 2.5 kb insert was identical to RanBPM cops5-uchl1 3 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 1 A PGP9.5 and JAB1 proteins coprecipitated when the JAB1 antibody was used cops5-uchl1-1 2 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 1 B PGP9.5 colocalizes with JAB1 both to the nucleus and cytoplasm of H1299 cell cops5-fbl 2 7 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization 2 H1299 cells were assayed for endogenous JAB1 and fibrillarin localization by immunofluorescence Fibrillarin is only present in the nucleolus in interphase cells. cdn1b-cops5 2 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 2 PGP9.5 and JAB1 were detected both in the cytoplasm and in the nucleoli, whereas p27Kip1 was detected primarily in the nucleus with strong staining to the nucleoli cdn1b-uchl1 2 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 2 PGP9.5 and JAB1 were detected both in the cytoplasm and in the nucleoli, whereas p27Kip1 was detected primarily in the nucleus with strong staining to the nucleoli cops5-cdkn1b 3 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3 A Endogenous JAB1-p27Kip1 complexes were immunoprecipitated from H1299 cell lysate with anti-JAB1 antibody and detected anti- p27Kip1 antibod cdkn1b-uchl1 3 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 3 C PGP9.5 is in the same complex with p27Kip1 in H1299 cell lysate as detected by immunoprecipitation using K25020 anti-p27Kip1 (K25020), but not M197 antibody The antibody used, K25020, mainly recognizes the nuclear form of p27. The M197 antibody which recognizes mainly the cytoplasmic form of p27 did not coimmunoprecipitate PGP9.5.