IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Int. J. Cancer (0020-7136) 2001 imex curation Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer shichijo@med.kurume-u.ac.jp 07-Sept-2007: Contacted by JYOTI. A human fetal liver cDNA library in a pACT vector (Clontech, Palo Alto, CA) was used. davet Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer HA-SART3 was coexpressed with FLAG-RNPS1 in 293T cells. The protein complexes were chemically crosslinked with 2-ME-cleavable DSP and were precipitated with an anti-FLAG MAb. Interacting proteins were detected with an anti-HA MAb in both reduced and nonreduced conditions. shichijo@med.kurume-u.ac.jp Accepted 2007-SEP-17 by DAVET. 07-Sept-2007: Contacted by JYOTI. Int. J. Cancer (0020-7136) 2001 davet Only protein-protein interactions imex curation Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Int. J. Cancer (0020-7136) 2001 imex curation Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer shichijo@med.kurume-u.ac.jp 07-Sept-2007: Contacted by JYOTI. A human fetal liver cDNA library in a pACT vector (Clontech, Palo Alto, CA) was used. davet Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer shichijo@med.kurume-u.ac.jp Accepted 2007-SEP-17 by DAVET. 07-Sept-2007: Contacted by JYOTI. Int. J. Cancer (0020-7136) 2001 davet Only protein-protein interactions imex curation Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Int. J. Cancer (0020-7136) 2001 imex curation Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer shichijo@med.kurume-u.ac.jp 07-Sept-2007: Contacted by JYOTI. A human fetal liver cDNA library in a pACT vector (Clontech, Palo Alto, CA) was used. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer shichijo@med.kurume-u.ac.jp Accepted 2007-SEP-17 by DAVET. 07-Sept-2007: Contacted by JYOTI. Int. J. Cancer (0020-7136) 2001 davet Only protein-protein interactions imex curation Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Int. J. Cancer (0020-7136) 2001 imex curation Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer shichijo@med.kurume-u.ac.jp 07-Sept-2007: Contacted by JYOTI. A human fetal liver cDNA library in a pACT vector (Clontech, Palo Alto, CA) was used. davet Only protein-protein interactions human-wi_38 Homo sapiens Caucasian fetal lung fibroblast cell line wi_38 WI39 human Caucasian fetal lung fibroblast cell line From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37C with periodic pH adjustments. Used in preparation of human virus vaccines. 2n = 46, diploid except at high passage number confocal microscopy confocal microscopy predetermined predetermined participant predetermined Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer VA-13 cells are also called WI-38 cells. (See Mao et al. Journal of Cell Science 118, 4427-4436 (2005)). shichijo@med.kurume-u.ac.jp Accepted 2007-SEP-17 by DAVET. 07-Sept-2007: Contacted by JYOTI. Int. J. Cancer (0020-7136) 2001 davet Only protein-protein interactions imex curation Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Binding of a SART3 tumor-rejection antigen to a pre-mRNA splicing factor RNPS1: a possible regulation of splicing by a complex formation. Int. J. Cancer (0020-7136) 2001 imex curation Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer shichijo@med.kurume-u.ac.jp 07-Sept-2007: Contacted by JYOTI. A human fetal liver cDNA library in a pACT vector (Clontech, Palo Alto, CA) was used. davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Harada K., Yamada A., Yang D., Itoh K., Shichijo S. Cancer - Interactions investigated in the context of cancer shichijo@med.kurume-u.ac.jp Accepted 2007-SEP-17 by DAVET. 07-Sept-2007: Contacted by JYOTI. A human fetal liver cDNA library in a pACT vector (Clontech, Palo Alto, CA) was used. Int. J. Cancer (0020-7136) 2001 davet Only protein-protein interactions imex curation rnps1_human RNA-binding protein with serine-rich domain 1 SR-related protein LDC2 RNPS1 LDC2 protein protein human Homo sapiens Human MDLSGVKKKSLLGVKENNKKSSTRAPSPTKRKDRSDEKSKDRSKDKGATKESSEKDRGRDKTRKRRSASSGSSSTRSRSSSTSSSGSSTSTGSSSGSSSSSASSRSGSSSTSRSSSSSSSSGSPSPSRRRHDNRRRSRSKSKPPKRDEKERKRRSPSPKPTKVHIGRLTRNVTKDHIMEIFSTYGKIKMIDMPVERMHPHLSKGYAYVEFENPDEAEKALKHMDGGQIDGQEITATAVLAPWPRPPPRRFSPPRRMLPPPPMWRRSPPRMRRRSRSPRRRSPVRRRSRSPGRRRHRSRSSSNSSR 930C9D36C2486144 sart3_human Squamous cell carcinoma antigen recognized by T-cells 3 KIAA0156 SART3 TIP110 Tat-interacting protein of 110 kDa p110 nuclear RNA-binding protein protein protein human Homo sapiens Human MATAAETSASEPEAESKAGPKADGEEDEVKAARTRRKVLSRAVAAATYKTMGPAWDQQEEGVSESDGDEYAMASSAESSPGEYEWEYDEEEEKNQLEIERLEEQLSINVYDYNCHVDLIRLLRLEGELTKVRMARQKMSEIFPLTEELWLEWLHDEISMAQDGLDREHVYDLFEKAVKDYICPNIWLEYGQYSVGGIGQKGGLEKVRSVFERALSSVGLHMTKGLALWEAYREFESAIVEAARLEKVHSLFRRQLAIPLYDMEATFAEYEEWSEDPIPESVIQNYNKALQQLEKYKPYEEALLQAEAPRLAEYQAYIDFEMKIGDPARIQLIFERALVENCLVPDLWIRYSQYLDRQLKVKDLVLSVHNRAIRNCPWTVALWSRYLLAMERHGVDHQVISVTFEKALNAGFIQATDYVEIWQAYLDYLRRRVDFKQDSSKELEELRAAFTRALEYLKQEVEERFNESGDPSCVIMQNWARIEARLCNNMQKARELWDSIMTRGNAKYANMWLEYYNLERAHGDTQHCRKALHRAVQCTSDYPEHVCEVLLTMERTEGSLEDWDIAVQKTETRLARVNEQRMKAAEKEAALVQQEEEKAEQRKRARAEKKALKKKKKIRGPEKRGADEDDEKEWGDDEEEQPSKRRRVENSIPAAGETQNVEVAAGPAGKCAAVDVEPPSKQKEKAASLKRDMPKVLHDSSKDSITVFVSNLPYSMQEPDTKLRPLFEACGEVVQIRPIFSNRGDFRGYCYVEFKEEKSALQALEMDRKSVEGRPMFVSPCVDKSKNPDFKVFRYSTSLEKHKLFISGLPFSCTKEELEEICKAHGTVKDLRLVTNRAGKPKGLAYVEYENESQASQAVMKMDGMTIKENIIKVAISNPPQRKVPEKPETRKAPGGPMLLPQTYGARGKGRTQLSLLPRALQRPSAAAPQAENGPAAAPAVAAPAATEAPKMSNADFAKLFLRK 06B26CEB8B19102A sart3-rnps1 5 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1380128]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 329-329, sequence length 305 [EBI-1380128]49-329 physical association physical association we employed a 2-hybrid screening in yeast for proteins interacting with SART3, and this method yielded a pre-mRNA splicing factor (RNA-binding protein prevalent during the S phase or RNA-binding protein with a serine-rich domain [RNPS1]) PREY gene AF274003 The predicted aa sequence of AF274003 corresponds to the range aa 25- 305 of Q15287. sart3-rnps1-1 3 7 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1380144]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 329-329, sequence length 305 [EBI-1380144]49-329 physical association physical association we employed a 2-hybrid screening in yeast for proteins interacting with SART3, and this method yielded a pre-mRNA splicing factor (RNA-binding protein prevalent during the S phase or RNA-binding protein with a serine-rich domain [RNPS1]) PREY gene AF274003 The predicted aa sequence of AF274003 corresponds to the range aa 25-305 of Q15287. sart3-rnps1-2 1 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region Lacking RS domain and part of the RRM domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association PREY gene AF274003 The predicted aa sequence of AF274003 corresponds to the range aa 25-305 of Q15287. We confirmed the binding of SART3 to RNPS1 in vitro using a recombinant SART3-glutathione-S-transferase fusion protein (GST-SART3) with the cell extract of FLAG-RNPS1-transfected 293T cells. This interaction occurs in the presence or absence of RNase A and DNase I. The data indicate that the RS domain and at least the C-terminal region of the RRM domains are not needed for the binding of RNPS1 to SART3. sart3-rnps1-3 2 6 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association PREY gene AF274003 The predicted aa sequence of AF274003 corresponds to the range aa 25-305 of Q15287. The binding of SART3 to RNPS1 was observed in the cell extract from the coexpressed cells. 3 Q15020; Q15287 transfection sart3-rnps1-4 4 6 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Q15287 gene AF274003 The predicted aa sequence of AF274003 corresponds to the range aa 25-305 of Q15287. 4 Cotransfection of the 2 genes changed the distribution pattern of SART3 from diffuse nucleoplasmic spreading to nuclear speckled regions in which DsRed-RNPS1 was colocalized as seen via double-label fluorescence, suggesting a complex formation of the 2 proteins in the nuclear speckled regions. Q15287; Q15020 transfection