IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. Nat. Cell Biol. (1465-7392) 2006 imex curation Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu Accepted 2006-AUG-24 by DAVET. Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. Nat. Cell Biol. (1465-7392) 2006 imex curation Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu Accepted 2006-AUG-24 by DAVET. 24-JAN-2007: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. The NuMA-related Mud protein binds Pins and regulates spindle orientation in Drosophila neuroblasts. Nat. Cell Biol. (1465-7392) 2006 imex curation Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions drome-embryonic_neuroblast Drosophila melanogaster embryonic_neuroblast CNS progenitor cells fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Siller KH., Cabernard C., Doe CQ. BioCreative - Critical Assessment of Information Extraction systems in Biology cdoe@uoregon.edu Accepted 2006-AUG-24 by DAVET. 24-JAN-2007: Contacted by JYOTI. Nat. Cell Biol. (1465-7392) 2006 Only protein-protein interactions imex curation q9vb22_drome pins raps Dmel\CG5692 PINS Pins Rad Raps rad rapsyn Dmel_CG5692 CG5692 RAPS dLGN dPins protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSLSASAENVSSLGLGSGGGGTTSHDGNSQQGSGSDGGSSMCLELALEGERLCKAGDCRAGVAFFQAAIQAGTEDLRTLSAIYSQLGNAYFYLGDYNKAMQYHKHDLTLAKSMNDRLGEAKSSGNLGNTLKVMGRFDEAAICCERHLTLARQLGDRLSEGRALYNLGNVYHAKGKHLGQRNPGKFGDDVKEALTRAVEFYQENLKLMRDLGDRGAQGRACGNLGNTYYLLGDFQAAIEHHQERLRIAREFGDRAAERRANSNLGNSHIFLGQFEDAAEHYKRTLALAVELGEREVEAQSCYSLGNTYTLLHEFNTAIEYHNRHLAIAQELGDRIGEARACWSLGNAHSAIGGHERALKYAEQHLQLAKELHDPVGESTARVNISDLRKLLGMPDSEPSPTEEEARSTASDHSASGNQSDGSENSQGRMVRVRRQSMEQLDLIKITPDGKRMQEEKLRAQATRKAKDDDFFEMLSRSQSKRMDDQRCSIKVNPAGAPAVATGATRKPLVQQNSLFVDPTNLPGLKSPSSANPSAIGHGPLARSATTTQQPDDDFLDMLMRCQGSRLEEQRSELPRPNVTMDAEAEAPPRSVPEAAVPGAPRGQTGRGATVPDEDFFSLIMKVQSGRMEDQRASIPFRNANNNNNSRSNNNGSAGGAGK DIP protein Q9VB22 original sequence version: 131 DIP protein Q9VB22 original sequence version: 109 DIP protein Q9VB22 original sequence version: 126 5D24B15B4FAE584F q8ir55_drome Dmel\CG12047 KS63 Mud NuMA mud NuMa CG12047 Dmel_CG12047 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDTRSWRKVLLQWIGECHFIESNYITLEQSDLDSFFSVFIQKIQETENVKGKNELQDQPTEQSPLVQEFLAHNYPEFIAQQDDKEVDLPLDCLYVYTLLLHYSCVKKPSLFFHNICNKLPELTQTCIASFFRETVDRLLTREYLSQAIANVAVVYRQGVSTSVSPCLPSSSLSPDPRSDDAPCPSTPSSSSSQPSSSTPQLRNHREQLRLNGCEMPPPSTPKTELLEQRTKELRGIRTQLEVVRYEKALLEEQQMEKDELIKVLNKEKMMAKMELEKLRNVKLTEEHHDNESHHIMPYEFEHMKGCLLKEIGLKESLIAEITDKLHDLRVENSELSEKLNLAGKRLLEYTDRIRFLESRVDDLTRIVSSRDVMISSLESDKQELDKCLKEARDDLHNRIEVLNASSDLLDCSLSPNTTPENLASSVIDKQLREKEHENAELKEKLLNLNNSQRELCQALSSFLQKHNIDHEFPVEWTSSSLLSTISAIESKFVNTLEKSTQMKKECDVQSVCVEKLLEKCKLLSVSLGCQPKELDGFEATIPEAMESGFESSRECETILSCCHMKVVDIASKNNDLELDNERLNDKCAELKSIIDRGDQHLADINLQLIEKEKQIKDVGAEIQELRKRNINLENMLSQIADKEASAASHAQHLKQCGELLRAKYEVCRNELIAKNAAQDELVRMMMVPDGETLNGRVRQLIDLEMMHDEHNKMYAQMLKQLNELSAKHDNMTHSHLDFVKRTEIELETKNAQIMAFDEHNNHFDRFLTRIFTLLRSRNCPKSTTMGSATNFLESMHIEKRFENIEMLIEGQLLSADDLKRELDDLRSKNEELAKQNINGIIKRNKFITSLEVNTEKVKQYITDLEEEAFKRKQKVVQLENTLSKEQSNAKEMAQRLDIAQQEIKDYHVEAIRFINTIRDRLQQDFNGVNTPQQLGTCMTEFLKMYDQMEVRYEESSSLVEKLTESQAKLEMQVAELQVELENKDTNQHSGALIKQLNDTIQNLEKVNAKLSEDNTVSHTVHSKLNESLLKAQKELDLRAKIIENLEASERNLSMKLCELKDLKNKLKSSDEKIAQIKETYEEQIKALQAKCDMEAKKNEHLERNQNQSLTQLKEDALENCVLMSTKLEELQAKLQEGQQLVDSQKLELDMNRKELALVKSAYEAQTKLSDDLQRQKESGQQLVDNLKVELEKERKELAHVNSAIGAQTKLSDDLECQKESGQQLVDNLKVELEKERKELAQVKSVIEAQTKLSDDLQREKESAQQLVDNLKVELDKERKELAQVNSAFEAQTKLSDDLQRQKESAQQLVDNLKVELDKERKELAQVNSAFEAQTKLSDDLQREKESAQQLVDNLKVELDKERKELAQVKSVIEAQTKLSDDLQRQKESAQQLVDNLKVELDKERKELAKVKSVIEAQTKLSDDLQRQKESAQQLEAQTKLSDDLQRQKESAQQLVDNLKVELDKERKELAQVKSVIEAQTKLSDDLQRQKESAQQLVDNLKMELDKERKELAQVKSAIGAQTKLSDDLECQKESVQQLVDNLKVELEKERKELAKVNSAFEAQTKLSDDLKLQKEDAQREVFLVKERLVKEKREFEVKLATLEDIIETLEMRCTQMEEERATAYEQINKLENRCQEKDNVKSSQLQVETFKVECLHHQLKSEMATHNSLVEDLNRKLAEKVSKLDFVQSRLMTEIAEHNQVKDQLAQITDIPKVVELQHRLEAETAEREEAQNKLAVVTGRLDEITRELDNARLEHGAQILRMEETAREVGNKNAELCELIEFYRNRVEALERLLLASNQELEELNSIQSNQAEGVRDLGDTYSAAEGRQTESDQDKERYQKLALDCKILQAKYRDAKDEIKRCEKKIKDQRLEMEGKLEKMKNKMRSLYTAEVTRMKEKQERDAAKSASELEALTAQNAKYEEHTRKLSNQIVRLNEKILEQQKQHAIISTNLRHLQMQPISETKPSSTTLTVSSSSSAPNDDWQPFKRPNVPSSNLAMEDEEGEVFNNTYLTDLKLGRVPADMTAEELIYRNSLQPPHLKSTYAAQYDLGSQDEDLKDGPHSLDDSMSALLSSSSTGTRKKSMGTHYKRPGPPTPSKNGGRLSFGSSEPPREILREFGDHNNTSKTPARFKFLTQRFSVGSSGLPRDEPIPQLPRRKRPNLLTGIHRRRLRHAVDIFCTSTPRKSRSYYDQQRMIGASDADKSEAVETEDEVIEVEPDAVPELEPLEDADQQGTPHLSTAALLALTKGNTRRLTGTTKSKKGRVSLSLHGNIFAKSRPASFVAGKRVQLRRKLRRDRIMGRFDEARHLDQMRLSHSAQAAKSPENNNYSLHNRNEEEHLPSENNVMGKTVVLVGKRRLSPVVSTTFNVEERSETSLLQQQFEHENCVTWAMHGGESVMMEETQNDWYFEQLCKETESTAPFQLQPLDYKPLDEEQVEPKFTQLVAASCSNITTTSCATNMTGASSCTVYSIGSVHMQPLPQINITYVQQPSDLQQRRPTRHRSLMAQCRRFLRHLSLGQRLVMGFALLVMVGLSLHLADKLVLVITGILSGMGLVFLTYSCPGRN D02AA4A8E38F3EA5 mud-raps-1 1 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 11 8 direct interaction direct interaction 1C The Mud 1825-1997 fragment from the Mud(2501) protein contains the NLM domain and is pelleted with GST-bound full-length Pins (amino acids 1-658; lanes 3,4) and GST-bound Pins amino-terminal TPR domain (amino acids 42-398; lanes 5,6) mud-raps-2 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 1D Mud and Pins immunoprecipitated (IP) from wild-type embryonic lysates with anti-Pins antibody but not with non-specific control antibodies. mud-raps-3 3 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component physical association physical association 2 & 3 In embryonic neuroblasts, we found that Mud and Pins were both enriched at the cortex over the apical centrosome/spindle pole from late interphase and up to the end of metaphase (Fig. 2a, b, d, e; Fig. 3a).