IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights. Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights. J. Biol. Chem. (0021-9258) 2006 imex curation Gengenbacher M., Fitzpatrick TB., Raschle T., Flicker K., Sinning I., Muller S., Macheroux P., Tews I., Kappes B. BioCreative - Critical Assessment of Information Extraction systems in Biology ivo.tews@bzh.uni-heidelberg.de 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions ecobb Escherichia coli (strain B / BL21) pull down pull down western blot western blot Immuno blot Immuno blot Gengenbacher M., Fitzpatrick TB., Raschle T., Flicker K., Sinning I., Muller S., Macheroux P., Tews I., Kappes B. BioCreative - Critical Assessment of Information Extraction systems in Biology ivo.tews@bzh.uni-heidelberg.de ivo.tews@bzh.uni-heidelberg.de Accepted 2006-AUG-2 by DAVET. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation pdx1_plaf7 Pyridoxal 5'-phosphate synthase subunit Pdx1 pdx1 PFF1025c protein protein plaf7 Plasmodium falciparum (isolate 3D7) MENHKDDAVLLKHGWCEMLKGGVIMDVKSVEQAKIAEEAGAIGVMVLENIPSELRNKEGVARSVDPSKVEEIKKCVSINVLAKVRIGHFVEAQILEELKIDMIDESEVLTIADEMHHIDKHKFKTPFVCGCTNLGEALRRISEGASMIRTKGEAGTGNIIEAIKHIRTVNNEIKYLCSLSDSEVYHFAKKINAPIDLVLLTKKLKRLPVVNFAAGGVATPADAAMCMQLGMDGVFVGSGIFESENPRKMAASIVSAVSNFNNPKILLDVSMNLGKAMCGSTRVSDKWKNKNEEHTKFLTPQ 87A2886A215ED219 pdx2_plaf7 Pyridoxal 5'-phosphate synthase subunit Pdx2 pdx2 Pyridoxal 5'-phosphate synthase glutaminase subunit PF11_0169 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MSEITIGVLSLQGDFEPHINHFIKLQIPSLNIIQVRNVHDLGLCDGLVIPGGESTTVRRCCAYENDTLYNALVHFIHVLKKPIWGTCAGCILLSKNVENIKLYSNFGNKFSFGGLDITICRNFYGSQNDSFICSLNIISDSSAFKKDLTAACIRAPYIREILSDEVKVLATFSHESYGPNIIAAVEQNNCLGTVFHPELLPHTAFQQYFYEKVKNYKYS 21636561DFBCB136 pdx1-pdx2-1 1 3 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role prey prey physical association physical association Paper is not very clear, but I think they have used strain 3D7 to clone protein. The presence of both proteins during the expression and purification procedure was verified by Western blotting using the affinity-purified Pdx1 antibody and a monoclonal His tag antibody for the detection of Pdx2 (Fig. 3B). Fig. 3B