IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p. The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p. J. Biol. Chem. (0021-9258) 2006 imex curation Polevoda B., Span L., Sherman F. BioCreative - Critical Assessment of Information Extraction systems in Biology TAP-purified Mrf1p and bacterially overexpressed His6-Mtq1p were used in the methyltransferase assays. Bogdan_Polevoda@urmc.Rochester.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Polevoda B., Span L., Sherman F. BioCreative - Critical Assessment of Information Extraction systems in Biology TAP-purified Mrf1p and bacterially overexpressed His6-Mtq1p were used in the methyltransferase assays. Bogdan_Polevoda@urmc.Rochester.edu Bogdan_Polevoda@urmc.Rochester.edu Accepted 2006-SEP-13 by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p. The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p. J. Biol. Chem. (0021-9258) 2006 imex curation Polevoda B., Span L., Sherman F. BioCreative - Critical Assessment of Information Extraction systems in Biology TAP-purified Mrf1p and bacterially overexpressed His6-Mtq1p were used in the methyltransferase assays. Bogdan_Polevoda@urmc.Rochester.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Polevoda B., Span L., Sherman F. BioCreative - Critical Assessment of Information Extraction systems in Biology TAP-purified Mrf1p and bacterially overexpressed His6-Mtq1p were used in the methyltransferase assays. Bogdan_Polevoda@urmc.Rochester.edu Bogdan_Polevoda@urmc.Rochester.edu Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation mtq1_yeast Mitochondrial MRF1 N(5)-glutamine methyltransferase MTQ1 N2420 YNL2420W YNL063W MTQ1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPRISTSLIRKASRIRPGLHLLLPECRTLEQAKLEYKWLTEELPPDKSIRWACLQRYKHVPLQYILRSQPFGALDIVCKPGVLIPRWETEEWVMAIIRALNNSMLSRHTIPLHICDTFTGTGCIALALSHGIANCTFTAIDVSTRAIKLVKENMLKNKVSGGKLVQHNILSSKASDEYPSHIDILTGNPPYIRKRDFNRDVKTSVKLFEPRLALVGELECYINLVNYWLPKTDSFFYEIGDVEQFNYVERRIKEDSYLSRIWSIGLKYDSNGKARVVYGFKATPKGRILHQIFASFGTIRHLATALSGHKANCN 2281F44B511EAA65 2281F44B511EAA65 rf1m_yeast Peptide chain release factor 1, mitochondrial MRF1 YGL143C protein protein yeast Saccharomyces cerevisiae Baker's yeast MWLSKFQFPSRSIFKGVFLGHKLPLLVRLTSTTTNSKSNGSIPTQYTELSPLLVKQAEKYEAELKDLDKDLSCGIHFDVNKQKHYAKLSALTDTFIEYKEKLNELKSLQEMIVSDPSLRAEAEQEYAELVPQYETTSSRLVNKLLPPHPFADKPSLLELRPGVGGIEAMIFTQNLLDMYIGYANYRKWKYRIISKNENESGSGIIDAILSIEEAGSYDRLRFEAGVHRVQRIPSTETKGRTHTSTAAVVVLPQIGDESAKSIDAYERTFKPGEIRVDIMRASGKGGQHVNTTDSAVRLTHIPSGIVVSMQDERSQHKNKAKAFTILRARLAEKERLEKEEKERKARKSQVSSTNRSDKIRTYNFPQNRITDHRCGFTLLDLPGVLSGERLDEVIEAMSKYDSTERAKELLESN AAAD4829748C7604 mtq1-mrf1 1 3 enzyme enzyme unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate unspecified role unspecified role region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast methylation methylation reaction methylation gln287 methylglutamine Labelled SAM used in the interaction. Figure 3 The in vitro assay was performed with partially purified Mtq1p. Coordinate of methylated Glutamine residue was determined from amino acid sequence and not given specifically in the text. Mtq1p Is Sufficient to Methylate Mrf1p in Vitro. mtq1-mrf1-1 2 3 enzyme enzyme unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 4 enzyme target enzyme target substrate substrate unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast methylation methylation reaction methylation gln287 methylglutamine SAM required for reaction. Figure 2 Coordinate of methylated Glutamine residue was determined from amino acid sequence and not given specifically in the text. Mtq1p Is Sufficient to Methylate Mrf1p in Vivo.