IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of a switch in neurotrophin signaling by selective tyrosine phosphorylation. Identification of a switch in neurotrophin signaling by selective tyrosine phosphorylation. J. Biol. Chem. (0021-9258) 2006 imex curation Arevalo JC., Pereira DB., Yano H., Teng KK., Chao MV. BioCreative - Critical Assessment of Information Extraction systems in Biology chao@saturn.med.nyu.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Arevalo JC., Pereira DB., Yano H., Teng KK., Chao MV. BioCreative - Critical Assessment of Information Extraction systems in Biology chao@saturn.med.nyu.edu Accepted 2006-JUL-31 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Identification of a switch in neurotrophin signaling by selective tyrosine phosphorylation. Identification of a switch in neurotrophin signaling by selective tyrosine phosphorylation. J. Biol. Chem. (0021-9258) 2006 imex curation Arevalo JC., Pereira DB., Yano H., Teng KK., Chao MV. BioCreative - Critical Assessment of Information Extraction systems in Biology chao@saturn.med.nyu.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Arevalo JC., Pereira DB., Yano H., Teng KK., Chao MV. chao@saturn.med.nyu.edu Accepted 2006-AUG-24 by DAVET. J. Biol. Chem. (0021-9258) 2006 Identification of a switch in neurotrophin signaling by selective tyrosine phosphorylation. Identification of a switch in neurotrophin signaling by selective tyrosine phosphorylation. J. Biol. Chem. (0021-9258) 2006 imex curation Arevalo JC., Pereira DB., Yano H., Teng KK., Chao MV. BioCreative - Critical Assessment of Information Extraction systems in Biology chao@saturn.med.nyu.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Arevalo JC., Pereira DB., Yano H., Teng KK., Chao MV. BioCreative - Critical Assessment of Information Extraction systems in Biology chao@saturn.med.nyu.edu Accepted 2006-JUL-31 by DAVET. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation crkl_rat Crk-like protein Crkl protein protein rat Rattus norvegicus (Rat) Rat MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPNPPMGSVSAPNLSTAEENLEYVRTLYDFPGNDAEDLPFKKGELLVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPTVASTPGAAINPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDDNE B6E2916741FE56E0 rpgf1_human Rap guanine nucleotide exchange factor 1 Guanine nucleotide-releasing factor 2 Protein C3G CRK SH3-binding GNRP RAPGEF1 GRF2 protein protein human Homo sapiens Human MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT DIP protein Q13905 original sequence version: 63 DIP protein Q13905-1 original sequence version: 106 E700631A0EE76CA1 kdis_rat Kinase D-interacting substrate of 220 kDa Kidins220 Arms Ankyrin repeat-rich membrane-spanning protein protein protein rat Rattus norvegicus (Rat) Rat MSVLISQSVINYVEEENIPALKALLEKCKDVDERNECGQTPLMLAAEQGNVEIVKELLKNGANCNLEDLDNWTALISASKEGHIHIVEELLKSGASLEHRDMGGWTALMWACYKGRTDVVELLLSHGANPSVTGLYSVYPIIWAAGRGHADIVHLLLQNGAKVNCSDKYGTTPLVWAARKGHLECVKHLLAMGADVDQEGANSMTALIVAVKGGYTQSVKEILKRNPNVNLTDKDGNTALMIASKEGHIEIVQDLLDAGTYVNIPDRSGDTVLIGAVRGGHVEIVRALLQKYADIDIRGQDNKTALYWAVEKGNATMVRDILQCNPDTEICTKDGETPLIKATKMRNIEVVELLLDKGAKVSAVDKKGDTPLHVAIRGRSRRLAELLLRNPKDGRLLYRPNKAGETPYNIDCSHQKSILTQIFGARHLSPTETDGDMLGYDLYSSALADILSEPTMQPPICVGLYAQWGSGKSFLLKKLEDEMKTFAGQQTEPLFQFSWLIVFLTLLLCGGLGLVFAFTVDTNLAIAISLSFLALIYIFFIVIYFGGRREGESWNWAWALSTRLARHIGYLELLFKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFRTEESQGKKKWKKTCCLPSFVIFLFIVGCIIAGITLLAIFRVDPKHLTVNAILISIASVVGLAFVLNCRTWWQVLDSLLNSQRKRLHSAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQTRLVVIIDGLDACEQDKVLQMLDTVRVLFSKGPFIAIFASDPHIIIKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLNSRGLSNARKFLVTSATNGDITCSDTTGTQEDTDRRVSQNSLGEMTKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQITFNWDRLASWINLTEQWPYRTSWLILYLEETEGLPDQMTLKTIYERISKNIPTTKDVEPLLEIDGDIRNFEVFLSSRTPVLVARDVKTFLPCTVNLDPKLREIIADVRAAREQINIGGLAYPPLPLHEGPPRPPSGYSQPASVCSSASFNGPFPGGVVSPQPHSSYYSGLSGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRSSVKTSLPRDQNNGLPCDSGFNKQRQAAVPATGSSLLLSSMTVDVVCEKLRQIEGLDQSMMPQYCTTIKKANINGRVLSQCNIDELKKEMAMNFGDWHLFRSMVLEMRSVESQVVPEDPRFLNENSSAPVPHGESARRSSHTELPLTELSSQTPYTLNFSFEELNTLGLDEGAPRHSNLSWQSQTRRTPSLSSLNSQDSSIEISKLTDKVQAEYRDAYREYIAQMSQLEGGTGSSTISGRSSPHSTYYIGQSSSGGSIHSTLEQERGKEGELKQEDGRKSFLMKRGDVIDYSSSGVSTNEASPLDPITEEDEKSDQSGSKLLPGKKSSERPSLFQTDLKLKGGGLRYQKLPSDEDESGTEESDNTPLLKDDKDKKAEGKAERVCKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSNESSPNHSLHNEAADDSQLEKANLIELEDEGHSGKRGMPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEESWPACQKAYNLNRTPSTVTLNNNTAPTNRANQNFDEIEGIRETSQVILRPGPSPNPTAVQNENLKSMAHKRSQRSSYTRLSKDASELHAASSESTGFGEERESIL 0CB2689A571F8AE4 crkl_human Crk-like protein CRKL protein protein human Homo sapiens Human MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE DIP protein P46109 original sequence version: 64 DIP protein P46109 original sequence version: 103 DIP protein P46109 original sequence version: 104 DIP protein P46109 original sequence version: 156 294CF1EE2CD44B81 ntrk1_rat High affinity nerve growth factor receptor Neurotrophic tyrosine kinase receptor type 1 p140-TrkA Slow nerve growth factor receptor Ntrk1 Trk Trka protein protein rat Rattus norvegicus (Rat) Rat MLRGQRHGQLGWHRPAAGLGGLVTSLMLACACAASCRETCCPVGPSGLRCTRAGTLNTLRGLRGAGNLTELYVENQRDLQRLEFEDLQGLGELRSLTIVKSGLRFVAPDAFHFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPLHCSCALLWLQRWEQEDLCGVYTQKLQGSGSGDQFLPLGHNNSCGVPSVKIQMPNDSVEVGDDVFLQCQVEGQALQQADWILTELEGTATMKKSGDLPSLGLTLVNVTSDLNKKNVTCWAENDVGRAEVSVQVSVSFPASVHLGKAVEQHHWCIPFSVDGQPAPSLRWFFNGSVLNETSFIFTQFLESALTNETMRHGCLRLNQPTHVNNGNYTLLAANPYGQAAASIMAAFMDNPFEFNPEDPIPVSFSPVDTNSTSRDPVEKKDETPFGVSVAVGLAVSAALFLSALLLVLNKCGQRSKFGINRPAVLAPEDGLAMSLHFMTLGGSSLSPTEGKGSGLQGHIMENPQYFSDTCVHHIKRQDIILKWELGEGAFGKVFLAECYNLLNDQDKMLVAVKALKETSENARQDFHREAELLTMLQHQHIVRFFGVCTEGGPLLMVFEYMRHGDLNRFLRSHGPDAKLLAGGEDVAPGPLGLGQLLAVASQVAAGMVYLASLHFVHRDLATRNCLVGQGLVVKIGDFGMSRDIYSTDYYRVGGRTMLPIRWMPPESILYRKFSTESDVWSFGVVLWEIFTYGKQPWYQLSNTEAIECITQGRELERPRACPPDVYAIMRGCWQREPQQRLSMKDVHARLQALAQAPPSYLDVLG DIP protein P35739 original sequence version: 73 DIP protein P35739 original sequence version: 108 D564E8801E8978F8 eea1 Rat Early endosome antigen 1 EEA1 protein protein rat Rattus norvegicus (Rat) Rat An amino acid sequence which is not represented in UniProt kidins220-trk-1 3 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Source of TrkA assumed from previous work by the author 1B To confirm the specificity of the phosphotyrosine antibody, we transfected HEK293 cells with wild type ARMS and ARMSY1096F together with TrkA. kidins220-crkl-1 1 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9EQG6:p.Tyr1095Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 16:00:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1095phe' Tue Jun 30 15:43:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1095Phe' 7 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 2B The tyrosine-phosphorylated GST-ARMS7.2 protein bound more CrkL protein compared with the non-phosphorylated protein (Fig. 2B, compare lanes 3 and 4). kidins220-crkl-2 1 4 unspecified role unspecified role prey prey Q5U2U2:p.Arg39Asn mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R N Wed May 02 19:36:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg39asn' Tue Jun 30 20:17:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg39Asn' in vitro In vitro 6 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9EQG6:p.Tyr1095Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 19:04:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1095phe' Tue Jun 30 20:17:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1095Phe' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2C To confirm the participation of SH2 domain of CrkL in ARMS-CrkL interaction [35S]methionine CrkL proteins were synthesized and tested for association with ARMS. I have written to the authors to confirm whether the CrkL is human or rat as i'm not 100% sure kidins220-crkl-3 1 5 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q9EQG6:p.Tyr1095Phe mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 19:58:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1095phe' Tue Jun 30 20:00:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1095Phe' 7 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association 7A In contrast to GST-wild type ARMS protein (7.2), a decrease in binding of C3G to CrkL was observed with GST-ARMSY1096F fusion protein (Fig. 7A). kidins220-eea1-1 2 9 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 4A Co-localization of ARMS and EEA1 using indirect immunofluorescence (Fig. 4A) also confirms these results. Nerve growth factor (NGF)