IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. J. Biol. Chem. (0021-9258) 2006 imex curation Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw Accepted 2006-JUL-24 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. J. Biol. Chem. (0021-9258) 2006 imex curation Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw Accepted 2006-JUL-24 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. J. Biol. Chem. (0021-9258) 2006 imex curation Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw Accepted 2006-JUL-24 by DAVET. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. J. Biol. Chem. (0021-9258) 2006 imex curation Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells protein kinase assay protein kinase assay predetermined predetermined participant predetermined Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw Accepted 2006-JUL-24 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. J. Biol. Chem. (0021-9258) 2006 imex curation Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells in gel kinase assay in-gel kinase assay in gel kinase assay predetermined predetermined participant predetermined Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw Accepted 2006-JUL-24 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. A pathway for tumor necrosis factor-alpha-induced Bcl10 nuclear translocation. Bcl10 is up-regulated by NF-kappaB and phosphorylated by Akt1 and then complexes with Bcl3 to enter the nucleus. J. Biol. Chem. (0021-9258) 2006 imex curation Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yeh PY., Kuo SH., Yeh KH., Chuang SE., Hsu CH., Chang WC., Lin HI., Gao M., Cheng AL. Apoptosis - Interactions involving proteins with a function related to apoptosis Protein-DNA cross-linked with 1% formaldehyde. Interactors isolated by precipitation with anti-RelA antibodies. andrew@ha.mc.ntu.edu.tw Accepted 2006-JUL-24 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation bcl10_human B-cell lymphoma/leukemia 10 BCL10 CIPER CLAP Cellular homolog of vCARMEN Mammalian CARD-containing adapter molecule E10 Cellular-E10 CARD-like apoptotic protein B-cell CLL/lymphoma 10 CED-3/ICH-1 prodomain homologous E10-like regulator CARD-containing molecule enhancing NF-kappa-B protein protein human Homo sapiens Human MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAKKILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDEVLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGESSTTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANSSEMFLPLRSRTVSRQ DIP protein O95999 original sequence version: 69 DIP protein O95999 original sequence version: 122 DIP protein O95999 original sequence version: 60 DIP protein O95999 original sequence version: 86 F87C97F2B784BA4B bcl3_human B-cell lymphoma 3 protein BCL3 BCL4 D19S37 Proto-oncogene BCL3 protein protein human Homo sapiens Human MPRCPAGAMDEGPVDLRTRPKAAGLPGAALPLRKRPLRAPSPEPAAPRGAAGLVVPLDPLRGGCDLPAVPGPPHGLARPEALYYPGALLPLYPTRAMGSPFPLVNLPTPLYPMMCPMEHPLSADIAMATRADEDGDTPLHIAVVQGNLPAVHRLVNLFQQGGRELDIYNNLRQTPLHLAVITTLPSVVRLLVTAGASPMALDRHGQTAAHLACEHRSPTCLRALLDSAAPGTLDLEARNYDGLTALHVAVNTECQETVQLLLERGADIDAVDIKSGRSPLIHAVENNSLSMVQLLLQHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSSLKNCHNDTPLMVARSRRVIDILRGKATRPASTSQPDPSPDRSANTSPESSSRLSSNGLLSASPSSSPSQSPPRDPPGFPMAPPNFFLPSPSPPAFLPFAGVLRGPGRPVPPSPAPGGS 385F5320DB72C0E0 nfkbia_human Human NFKBIA (I-kappa-B alpha) gene dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human ikba_human_gene NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 gene gene human Homo sapiens Human bcl10_human_gene Human Bcl10 gene gene gene human Homo sapiens Human akt1_human RAC-alpha serine/threonine-protein kinase AKT1 PKB RAC RAC-PK-alpha Protein kinase B Proto-oncogene c-Akt Protein kinase B alpha protein protein human Homo sapiens Human MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLMLDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA DIP protein P31749 original sequence version: 184 DIP protein P31749 original sequence version: 168 DIP protein P31749 original sequence version: 102 DIP protein P31749 original sequence version: 110 DIP protein P31749 original sequence version: 160 DIP protein P31749 original sequence version: 136 DIP protein P31749 original sequence version: 175 DIP protein P31749 original sequence version: 201 DIP protein P31749 original sequence version: 166 DIP protein P31749 original sequence version: 178 DIP protein P31749-1 original sequence version: 194 DIP protein P31749 original sequence version: 106 DIP protein P31749 original sequence version: 141 6EAFF4F8AD436714 tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS DIP protein Q04206 original sequence version: 170 DIP protein Q04206 original sequence version: 173 DIP protein Q04206 original sequence version: 99 DIP protein Q04206-1 original sequence version: 99 DIP protein Q04206 original sequence version: 168 DIP protein Q04206 original sequence version: 128 DIP protein Q04206 original sequence version: 131 DIP protein Q04206 original sequence version: 153 DIP protein Q04206 original sequence version: 185 DIP protein Q04206 original sequence version: 181 DIP protein Q04206 original sequence version: 183 8147A6AF9F2445C6 bcl10dna-rela-1 3 11 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait association association The results of the p65 ChIP assay indicated that TNFalpha induced a strong but shorter binding of p65 to the Bcl10 genomic fragment; in contrast, p65 bound to the IkappaBalpha genomic fragment for a longer period (Fig. 3A). 3A TNFalpha rela-nfkbia-9 3 10 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait association association The results of the p65 ChIP assay indicated that TNFalpha induced a strong but shorter binding of p65 to the Bcl10 genomic fragment; in contrast, p65 bound to the IkappaBalpha genomic fragment for a longer period (Fig. 3A). 3A TNFalpha bcl10dna-rela-2 1 11 unspecified role unspecified role neutral component neutral component region -270 to -250 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association EMSA was used to verify that the core sequence responsible for NF-kappaB binding resides in the BCL10 or I-kappa-Balpha gene. 3B, 3C TNFalpha Interaction competed out using unlabelled probe nfkbiadna-rela-3 1 9 unspecified role unspecified role neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined kappab region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association EMSA was used to verify that the core sequence responsible for NF-kappaB binding resides in the BCL10 or I-kappa-Balpha gene. 3B TNFalpha bcl10-akt1-1 2 12 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association The possible interaction between Bcl10 and Akt1 was identified by reciprocal co-immunoprecipitation, followed by Western blotting. 5B TNFalpha bcl10-akt1-2 2 12 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 5B The possible interaction between Bcl10 and Akt1 was identified by reciprocal co-immunoprecipitation, followed by Western blotting. TNFalpha bcl10-akt1-3 6 7 unspecified role unspecified role bait bait c-terminal region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association Nickel-agarose-Bcl10 pull-down experiments were performed to identify the interaction of Bcl10 with Akt1. 6A bcl10-akt1-4 4 7 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 12 enzyme enzyme unspecified role unspecified role human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association Akt1 Phosphorylates the cl10 C-terminal Ser218 and Ser231 Residues 6B Phosphorylation of BCL10 at Ser-218 and Ser-231 akt1-akt1-3 5 12 putative self putative self putative self putative self human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells phosphorylation phosphorylation reaction phosphorylation true 6D and an autophosphorylated protein with a molecular mass corresponding to that of Akt1 was observed in all pull-down complexes. bcl10-bcl3-1 2 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Next, reciprocal co-immunoprecipitation, followed by Western blotting, demonstrated that the Bcl10-Bcl3 complex existed in the nucleus after TNFalpha treatment. 7D TNFalpha bcl10-bcl3-2 2 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Next, reciprocal co-immunoprecipitation, followed by Western blotting, demonstrated that the Bcl10-Bcl3 complex existed in the nucleus after TNFalpha treatment. 7D TNFalpha bcl10-bcl3-3 6 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O95999:p.Ser218Asp mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 17:03:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser218asp' Tue Jun 30 17:31:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser218Asp' O95999:p.Ser231Asp mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:14:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser231asp' Tue Jun 30 15:57:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser231Asp' 8 unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association 7E TNFalpha