IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The plant plasma membrane Ca2+ pump ACA8 contains overlapping as well as physically separated autoinhibitory and calmodulin-binding domains. The plant plasma membrane Ca2+ pump ACA8 contains overlapping as well as physically separated autoinhibitory and calmodulin-binding domains. J. Biol. Chem. (0021-9258) 2006 imex curation Baekgaard L., Luoni L., De Michelis MI., Palmgren MG. BioCreative - Critical Assessment of Information Extraction systems in Biology The authors refer to this assay as a CaM overaly assay the nearest eqivilent I can find in inetraction detection is Far Weastern palmgren@kvl.dk 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Baekgaard L., Luoni L., De Michelis MI., Palmgren MG. BioCreative - Critical Assessment of Information Extraction systems in Biology The authors refer to this assay as a CaM overaly assay the nearest eqivilent I can find in inetraction detection is Far Weastern palmgren@kvl.dk Accepted 2006-AUG-29 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The plant plasma membrane Ca2+ pump ACA8 contains overlapping as well as physically separated autoinhibitory and calmodulin-binding domains. The plant plasma membrane Ca2+ pump ACA8 contains overlapping as well as physically separated autoinhibitory and calmodulin-binding domains. J. Biol. Chem. (0021-9258) 2006 imex curation Baekgaard L., Luoni L., De Michelis MI., Palmgren MG. BioCreative - Critical Assessment of Information Extraction systems in Biology The authors refer to this assay as a CaM overaly assay the nearest eqivilent I can find in inetraction detection is Far Weastern palmgren@kvl.dk 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Baekgaard L., Luoni L., De Michelis MI., Palmgren MG. BioCreative - Critical Assessment of Information Extraction systems in Biology The authors refer to this assay as a CaM overaly assay the nearest eqivilent I can find in inetraction detection is Far Weastern palmgren@kvl.dk Accepted 2006-AUG-29 by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation aca8_arath Calcium-transporting ATPase 8, plasma membrane-type Ca(2+)-ATPase isoform 8 ACA8 At5g57110 MUL3.5 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MTSLLKSSPGRRRGGDVESGKSEHADSDSDTFYIPSKNASIERLQQWRKAALVLNASRRFRYTLDLKKEQETREMRQKIRSHAHALLAANRFMDMGRESGVEKTTGPATPAGDFGITPEQLVIMSKDHNSGALEQYGGTQGLANLLKTNPEKGISGDDDDLLKRKTIYGSNTYPRKKGKGFLRFLWDACHDLTLIILMVAAVASLALGIKTEGIKEGWYDGGSIAFAVILVIVVTAVSDYKQSLQFQNLNDEKRNIHLEVLRGGRRVEISIYDIVVGDVIPLNIGNQVPADGVLISGHSLALDESSMTGESKIVNKDANKDPFLMSGCKVADGNGSMLVTGVGVNTEWGLLMASISEDNGEETPLQVRLNGVATFIGSIGLAVAAAVLVILLTRYFTGHTKDNNGGPQFVKGKTKVGHVIDDVVKVLTVAVTIVVVAVPEGLPLAVTLTLAYSMRKMMADKALVRRLSACETMGSATTICSDKTGTLTLNQMTVVESYAGGKKTDTEQLPATITSLVVEGISQNTTGSIFVPEGGGDLEYSGSPTEKAILGWGVKLGMNFETARSQSSILHAFPFNSEKKRGGVAVKTADGEVHVHWKGASEIVLASCRSYIDEDGNVAPMTDDKASFFKNGINDMAGRTLRCVALAFRTYEAEKVPTGEELSKWVLPEDDLILLAIVGIKDPCRPGVKDSVVLCQNAGVKVRMVTGDNVQTARAIALECGILSSDADLSEPTLIEGKSFREMTDAERDKISDKISVMGRSSPNDKLLLVQSLRRQGHVVAVTGDGTNDAPALHEADIGLAMGIAGTEVAKESSDIIILDDNFASVVKVVRWGRSVYANIQKFIQFQLTVNVAALVINVVAAISSGDVPLTAVQLLWVNLIMDTLGALALATEPPTDHLMGRPPVGRKEPLITNIMWRNLLIQAIYQVSVLLTLNFRGISILGLEHEVHEHATRVKNTIIFNAFVLCQAFNEFNARKPDEKNIFKGVIKNRLFMGIIVITLVLQVIIVEFLGKFASTTKLNWKQWLICVGIGVISWPLALVGKFIPVPAAPISNKLKVLKFWGKKKNSSGEGSL 2E86572F44DEA8A5 calm_bovin Calmodulin CALM CAM protein protein bovin Bos taurus (Bovine) Bovine MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62157 original sequence version: 125 DIP protein P62157 original sequence version: 58 DIP protein P62157 original sequence version: null 6B4BC3FCDE10727B aca8-cam 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9LF79:p.Trp47Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W A Wed May 02 16:28:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp47ala' Tue Jun 30 17:21:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp47Ala' Q9LF79:p.Arg48Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:06:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg48ala' Tue Jun 30 18:05:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg48Ala' Q9LF79:p.Ala56Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A S Wed May 02 17:23:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala56ser' Tue Jun 30 16:37:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala56Ser' Q9LF79:p.Phe60Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain F A Wed May 02 14:24:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe60ala' Tue Jun 30 15:47:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe60Ala' Q9LF79:p.Ala51Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A S Wed May 02 18:33:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala51ser' Tue Jun 30 19:48:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala51Ser' Q9LF79:p.Val53Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A Wed May 02 17:39:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val53ala' Tue Jun 30 19:28:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val53Ala' Q9LF79:p.Leu54Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:46:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu54ala' Tue Jun 30 15:37:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu54Ala' Q9LF79:p.Arg58Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:54:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg58ala' Tue Jun 30 16:26:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg58Ala' Q9LF79:p.Arg61Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:14:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg61ala' Tue Jun 30 18:58:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg61Ala' Q9LF79:p.Leu64Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Wed May 02 14:30:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu64ala' Tue Jun 30 18:08:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu64Ala' Q9LF79:p.Lys67Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 18:40:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys67ala' Tue Jun 30 16:42:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys67Ala' Q9LF79:p.Lys68Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 20:06:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys68ala' Tue Jun 30 16:33:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys68Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction The authors state that mutations try47ala, arg48ala, ala56ser and phe60ala severly reduced binding of bovine calmodulin (P62157) to ACA8 protein (Q9LF79) . Whereas the remaining muations only reduced inetraction between the two proteins. Figure 5 Cam binding to wild type and mutated N terminal of ACA8 measured by CaM overlay assay. Interactions occured in the presence of 1mM calcium chloride aca8-cam-1 1 3 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9LF79:p.Trp47Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W A Wed May 02 18:57:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp47ala' Tue Jun 30 17:06:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp47Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction kinetic constants could not be determined because signal was too low. Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface palsmon resonance. aca8-cam-2 1 3 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9LF79:p.Arg48Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:42:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg48ala' Tue Jun 30 17:26:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg48Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 158.0 SE = 4.1 aca8-cam-3 1 3 unspecified role unspecified role bait bait Q9LF79:p.Leu44Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:06:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu44ala' Tue Jun 30 17:57:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu44Ala' n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 46.4 SE = 0.59 aca8-cam-4 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9LF79:p.Ala51Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A S Wed May 02 16:13:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala51ser' Tue Jun 30 18:58:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala51Ser' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 45.0 SE = 0.8 aca8-cam-5 1 3 unspecified role unspecified role bait bait Q9LF79:p.Val53Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A Wed May 02 15:35:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val53ala' Tue Jun 30 16:37:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val53Ala' n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 65.1 SE = 1.17 aca8-cam-6 1 3 unspecified role unspecified role bait bait Q9LF79:p.Ala56Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain A S Wed May 02 14:47:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala56ser' Tue Jun 30 16:52:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala56Ser' n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 268.1 SE = 7.92 aca8-cam-7 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait Q9LF79:p.Arg58Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 18:43:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg58ala' Tue Jun 30 19:32:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg58Ala' n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 57.8 SE = 0.82 aca8-cam-8 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9LF79:p.Phe60Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:27:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe60ala' Tue Jun 30 16:35:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe60Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kinetic constants could not be determined because signal was too low aca8-cam-9 1 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9LF79:p.Arg61Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:59:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg61ala' Tue Jun 30 20:10:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg61Ala' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 49.9 SE = 1.09 aca8-cam-10 1 3 unspecified role unspecified role bait bait Q9LF79:p.Leu64Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:49:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu64ala' Tue Jun 30 20:06:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu64Ala' n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 79,5 SE = 2.95 aca8-cam-11 1 3 unspecified role unspecified role bait bait Q9LF79:p.Lys67Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 17:24:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys67ala' Tue Jun 30 16:32:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys67Ala' n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 55.7 SE = 0.95 aca8-cam-12 1 3 unspecified role unspecified role bait bait Q9LF79:p.Lys68Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:18:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys68ala' Tue Jun 30 19:26:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys68Ala' n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 58.0 SE = 0.86 aca8-cam-13 1 3 unspecified role unspecified role bait bait Q9LF79:p.Leu54Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:13:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu54ala' Tue Jun 30 16:34:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu54Ala' n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey direct interaction direct interaction Figure 6 Table 2 binding of CaM to recombinant N termini of ACA8 was further analysed by surface plasmon resonance Kd = 51.3 SE = 0.63