IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The loss of PIN1 deregulates cyclin E and sensitizes mouse embryo fibroblasts to genomic instability. The loss of PIN1 deregulates cyclin E and sensitizes mouse embryo fibroblasts to genomic instability. J. Biol. Chem. (0021-9258) 2006 imex curation Yeh ES., Lew BO., Means AR. BioCreative - Critical Assessment of Information Extraction systems in Biology No Cyclin E identified as human cyclin E1 based on previous work of contributer. Residue numbering in the paper is based on version 1 of the sequence P24864.1 and has been updated to P24864.2. Data on Xenopus or endogenous human Cyclin E could not be entered as this could not be attributed to E1 or E2. means001@mc.duke.edu 14-DEC-2006: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Yeh ES., Lew BO., Means AR. BioCreative - Critical Assessment of Information Extraction systems in Biology No Cyclin E identified as human cyclin E1 based on previous work of contributer. Residue numbering in the paper is based on version 1 of the sequence P24864.1 and has been updated to P24864.2. Data on Xenopus or endogenous human Cyclin E could not be entered as this could not be attributed to E1 or E2. means001@mc.duke.edu Accepted 2006-JUL-18 by DAVET. 14-DEC-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation The loss of PIN1 deregulates cyclin E and sensitizes mouse embryo fibroblasts to genomic instability. The loss of PIN1 deregulates cyclin E and sensitizes mouse embryo fibroblasts to genomic instability. J. Biol. Chem. (0021-9258) 2006 imex curation Yeh ES., Lew BO., Means AR. BioCreative - Critical Assessment of Information Extraction systems in Biology No Cyclin E identified as human cyclin E1 based on previous work of contributer. Residue numbering in the paper is based on version 1 of the sequence P24864.1 and has been updated to P24864.2. Data on Xenopus or endogenous human Cyclin E could not be entered as this could not be attributed to E1 or E2. means001@mc.duke.edu 14-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yeh ES., Lew BO., Means AR. BioCreative - Critical Assessment of Information Extraction systems in Biology No Cyclin E identified as human cyclin E1 based on previous work of contributer. Residue numbering in the paper is based on version 1 of the sequence P24864.1 and has been updated to P24864.2. Data on Xenopus or endogenous human Cyclin E could not be entered as this could not be attributed to E1 or E2. means001@mc.duke.edu Accepted 2006-JUL-18 by DAVET. 14-DEC-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation pin1_human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 Rotamase Pin1 Peptidyl-prolyl cis-trans isomerase Pin1 PIN1 protein protein human Homo sapiens Human MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE DIP protein Q13526 original sequence version: 65 DIP protein Q13526 original sequence version: 64 DIP protein Q13526 original sequence version: 163 DIP protein Q13526 original sequence version: 156 DIP protein Q13526 original sequence version: 117 35391AF40B7D1E13 q9i9k6_xenla Peptidyl-prolyl cis-trans isomerase pin1.L Pin1 pin1 XELAEV_18018988mg protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MADEEKLPPGWEKRMSRSSGRVYYFNHMTNASQWERPIAGGKNGQGEPGKVRCSHLLVKHNQSRRPSSWRQDKITRSKDEALELINGYIQKMKSGDEDFESLASQFSDCSSAKAGGDLGSFGKGAMQKPFEDASFALRPGEMSGPVFTDSGIHIILRTE DIP protein Q9I9K6 original sequence version: 71 4E54F66F16C9DF1C ccne1_human G1/S-specific cyclin-E1 CCNE1 CCNE protein protein human Homo sapiens Human MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA DIP protein P24864 original sequence version: 114 DIP protein P24864 original sequence version: 150 424DF0B253B7047E cdk2_human Cyclin-dependent kinase 2 CDK2 p33 protein kinase Cell division protein kinase 2 CDKN2 protein protein human Homo sapiens Human MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSFPKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL DIP protein P24941 original sequence version: 142 DIP protein P24941 original sequence version: 118 DIP protein P24941 original sequence version: 143 DIP protein P24941 original sequence version: 144 DIP protein P24941 original sequence version: 152 DIP protein P24941 original sequence version: 182 DIP protein P24941-1 original sequence version: 204 DIP protein P24941 original sequence version: 90 DIP protein P24941 original sequence version: 190 DIP protein P24941 original sequence version: 168 F90A0F4E70910B51 ccne1-pin1-1 1 5 unspecified role unspecified role prey prey P24864:p.Ser399Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:32:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser399ala' Tue Jun 30 17:28:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser399Ala' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 1C GST-Pin1 also binds Myc-tagged cyclin E. cdk2-pin1-2 1 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 1D, 1E Pin1 might bind to the hetrodimer in a manner that requires an active CDK2 protein to catalyze the phosphorylation of Ser384 on cyclin E. Roscovitine, inhibitor of CDK2 activity. cdk2-pin1-3 2 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P24864:p.Ser399Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 20:18:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser399ala' Tue Jun 30 17:55:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser399Ala' 6 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. association association 1F P24864 transfection; cdk2-ccne1-3 2 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association 1F P24864 transfection;